The worldwide problem of infectious diseases has appeared in recent years, and antimicrobial agents are crucial in reducing disease emergence. Nevertheless, the development and distribution of multidrug-resistant (MDR) strains in pathogenic bacteria, such as Escherichia coli, Staphylococcus aureus, Salmonella Typhi and Citrobacter koseri, has become a major society health hazard. Essential oils could serve as a promising tool as a natural drug in fighting the problem with these bacteria. The current study aimed to investigate the antimicrobial effectiveness of tea tree (Melaleuca alternifolia (Maiden and Betche) Cheel), rosemary (Rosmarinus officinalis L.), eucalyptus (Eucalyptus obliqua L’Hér.), and lavender (Lavandula angustifolia Mill) essential oils. The antimicrobial properties of essential oils were screened against four pathogenic bacteria, E. coli, S. aureus, S. Tyhpi, and C. koseri, and two reference bacterial strains, while for the testing, the agar well diffusion method was used. Gas chromatography (GC) and gas chromatography–mass spectrometric (GC–MSD) analyses were performed on essential oils. The obtained results showed that M. alternifolia essential oil is the richest in terpinen-4-ol, R. officinalis and E. oblique essential oils in 1,8-cineole, and L. angustifolia essential oil in α-terpinyl acetate. In addition, the main bioactive compounds present in the essential oil of tea tree are rich in α-pinene (18.38%), limonene (7.55%) and γ-terpinene (14.01%). The essential oil of rosemary is rich in α-pinene (8.38%) and limonene (11.86%); eucalyptus essential oil has significant concentrations of α-pinene (12.60%), p-cymene (3.24%), limonene (3.87%), and γ-terpinene (7.37%), while the essential oil of lavender is rich in linalool (10.71%), linalool acetate (9.60%), α-terpinyl acetate (10.93%), and carbitol (13.05%) bioactive compounds, respectively. The obtained results from the in vitro study revealed that most of the essential oils exhibited antimicrobial properties. Among the tested essential oils, tea tree was discovered to demonstrate the strongest antimicrobial activity. The recorded MIC of S. Typhi was 6.2 mg/mL, 3.4 mg/mL of C. koseri, 3.1 mg/mL of E. coli, and 2.7 mg/mL of E. coli ATCC 25922, compared to M. alternifolia. Similarly, only S. aureus ATCC 25923 showed antimicrobial activity towards R. officinalis (1.4 mg/mL), E. oblique (2.9 mg/mL), and L. angustifolia (2.1 mg/mL). Based on the obtained results, it is possible to conclude that tea tree essential oil might be used as an ecological antimicrobial in treating infectious diseases caused by the tested pathogens.

Antibiotics are widely recognized as significant chemical pollutants that enter the environment and ultimately the food chain. They are extensively used in both aquaculture and terrestrial animal breeding. Antibiotic residues in cultured fish pose significant public health risks, including the potential for antimicrobial resistance and adverse health outcomes. This review examines the widespread use of antibiotics in aquaculture, highlighting key challenges such as the lack of reliable data on antibiotic consumption in many regions as well as variability in regulatory enforcement. While strict regulations in European countries help to mitigate risks, the growing, often unregulated use of antibiotics in low- and middle-income countries exacerbates concerns over food safety. This paper provides an in-depth analysis of global regulatory frameworks and the impact of antibiotic residues on public health, and it offers recommendations for improving the monitoring, regulation, and responsible use of antibiotics in aquaculture in order to ensure safer food products from farmed fish. It contributes to a deeper understanding of the global scope of antibiotic misuse in aquaculture and points to an urgent need for more effective management practices.

In this paper, the electrical conductivity and concentration of free acids (acidity) of various types of honey collected from the area of Vojvodina was investigated. Physicochemical analyzes of parameters such as electrical conductivity and acidity of honey have an important role in defining the overall properties of honey and assessing the quality of honey. A total of 55 samples of honey were examined. Out of the total number of samples, 5 samples did not meet the requirements of the Republic of Serbia Regulations on quality of honey due to inadequate electrical conductivity. Free acid values measured in tested samples ranged from 1.5 to 30 mEq / kg, and the values of electrical conductivity were in the range between 0.08 and 1.99 mS/cm. Our assumption that there is a correlation between the tested parameters has been confirmed only in meadow and polyfloral honey. Further research on the physicochemical properties of Serbian honey is recommended and important in order to establish certification marks and criteria for assessing the quality of Serbian honey.

Honey’s unique qualities should last for several years when properly stored. Therefore, it is up to manufacturers to choose the right shelf life for their product while also considering the product’s nature. Physicochemical parameters (water content, electrical conductivity, free acidity, pH, ash, water-insoluble matter, hydroxymethylfurfural (HMF), sugar content and composition, and diastase activity) were analyzed in 24 samples of sunflower honey collected from several localities in Vojvodina, Serbia. Crystallization indices were also calculated. Furthermore, the impact of eighteen months of room temperature storage (22 ± 2 °C) in a dark place on selected physicochemical parameters (water, HMF, diastase activity, pH value, and free acidity) was investigated. The results of the initial test indicated that the tested samples of sunflower honey from Vojvodina is of good quality because the parameters under examination revealed results that were within the legal bounds of both national and European legislations. Eighteen months of storage at room temperature reduced diastase activity by 2 times, increased HMF content by about 17 times, and decreased the pH value of honey from a mean value of 3.66 to 3.56. The water content was relatively stable at 17.01% before storage and 16.29% after storage. The storage of sunflower honey did not have an impact on the free acidity.

Food borne transmission of antibiotic resistant strains of Escherichia coli from contaminated food has been recognised as an important hazard for human health in the past few decades and pathogenic strains of E. coli have long been considered as serious zoonotic hazards. Poultry meat is attractive for consumers worldwide, so the measures to preserve the safety of poultry meat are important issues. Tetracyclines are one of the most frequently used antibiotics in poultry farming and are still used in human medicine. The widespread use of tetracyclines in poultry farming may have resulted in the development and transmission of resistance strains from poultry to humans via the food chain. The relationship between the use of tetracyclines and the presence of resistant strains of E. coli in poultry meat, mainly due to the irregular use of antibiotics, is reviewed in this paper. The need for prudent use of antibiotics, particularly those which are used in human medicine is highlighted. Continuous monitoring and reporting on incidents in the future should improve the current regulations.

The symbiotic relationship between bees and the environment underscores the potential of apiculture as a sustainable practice. Bees, as pollinators, play a crucial role in ecosystem health and biodiversity conservation. Their pollination services are essential for the reproduction of numerous plant species, including many crops that constitute the backbone of agricultural economies. Beekeeping can diversify income sources, reducing dependence on single crops and enhancing household resilience to economic shocks. The aim of this paper is to explore the multifaceted role of apiculture as a tool for sustainable rural development. In conclusion, apiculture holds immense promise as a tool for sustainable rural development, offering a pathway towards economic prosperity, social inclusion, and environmental stewardship. By harnessing the synergies between bees, biodiversity, and community development, beekeeping has the potential to transform rural landscapes and livelihoods. However, realizing this potential requires concerted efforts to address the challenges and barriers that hinder the widespread adoption of beekeeping practices. Through collaborative action and integrated approaches, it can unlock the transformative power of apiculture and build resilient, vibrant, and sustainable rural communities for generations to come.