Owing to the reduction of population density and/or the environmental changes it induces, selective logging could affect the demography, reproductive biology and evolutionary potential of forest trees. This is particularly relevant in tropical forests where natural population densities can be low and isolated trees may be subject to outcross pollen limitation and/or produce low-quality selfed seeds that exhibit inbreeding depression. Comparing reproductive biology processes and genetic diversity of populations at different densities can provide indirect evidence of the potential impacts of logging. Here, we analysed patterns of genetic diversity, mating system and gene flow in three Central African populations of the self-compatible legume timber species Erythrophleum suaveolens with contrasting densities (0.11, 0.68 and 1.72 adults per ha). The comparison of inbreeding levels among cohorts suggests that selfing is detrimental as inbred individuals are eliminated between seedling and adult stages. Levels of genetic diversity, selfing rates (∼16%) and patterns of spatial genetic structure (Sp ∼0.006) were similar in all three populations. However, the extent of gene dispersal differed markedly among populations: the average distance of pollen dispersal increased with decreasing density (from 200 m in the high-density population to 1000 m in the low-density one). Overall, our results suggest that the reproductive biology and genetic diversity of the species are not affected by current logging practices. However, further investigations need to be conducted in low-density populations to evaluate (1) whether pollen limitation may reduce seed production and (2) the regeneration potential of the species.

Combining phenotypic and genetic characteristics in a genetic variation study is of paramount importance to effectively orient the selection of producers’ elite trees in a seed orchard. In total, 28 phenotypic characteristics and 16 microsatellite loci were used to analyze the clonal genetic variation, to characterize the genetic diversity, and to refine the genetic classifications of 110 Pinus koraiensis clones grown in the Naozhi orchard in northeastern China. All clones were significantly different in most traits. Most of the phenotypic characteristics showed great genetic variation among clones, while the genotypic differentiation was weak between the selection sites of clones. The SSR markers showed a relatively high level of genetic diversity (Na = 4.67 ± 0.43, Ne = 2.916 ± 0.18, I = 1.15 ± 0.07, Ho = 0.69 ± 0.04, He = 0.62 ± 0.02, and mean polymorphic information content (PIC) of 0.574), with higher heterozygosity as an indication of a lower probability of inbreeding events in the orchard. Despite weak correlation coefficients between dissimilarity matrices (r(A/B), range equal to 0.022, p-value < 0.001), the genetic and phenotypic classifications congruently subdivided all the clones into three major groups. The patterns of phenotypic trait variations and genetic diversity are valuable to effectively select materials in breeding programs of P. koraiensis.

In order to determine suitable traits for selecting high-wood-yield Korean pine materials, eleven morphological characteristics (tree height, basal diameter, diameter at breast height, diameter at 3 meter height, stem straightness degree, crown breadth, crown height, branch angle, branch number per node, bark thickness, and stem volume) were investigated in a 38-year-old Korean pine clonal trial at Naozhi orchard. A statistical approach combining variance and regression analysis was used to extract appropriate traits for selecting elite clones. Results of variance analysis showed significant difference in variance sources in most of the traits, except for the stem straightness degree, which had a p-value of 0.94. Moderate to high coefficients of variation and clonal repeatability ranged from 10.73% to 35.45% and from 0.06% to 0.78%, respectively. Strong significant correlations on the phenotypic and genotypic levels were observed between the straightness traits and tree volume, but crown breadth was weakly correlated to the volume. Four principal components retaining up to 80% of the total variation were extracted, and stem volume, basal diameter, diameter at breast height, diameter at 3 meter height, tree height, and crown height displayed high correlation to these components (r ranged from 0.76 to 0.98). Based on the Type III sum of squares, tree height, diameter at breast height, and branch number showed significant information to explain the clonal variability based on stem volume. Using the extracted characteristics as the selection index, six clones (PK105, PK59, PK104, PK36, PK28, and K101) displayed the highest Qi values, with a selection rate of 5% corresponding to the genetic gain of 42.96% in stem volume. This study provides beneficial information for the selection of multiple traits for genetically improved genotypes of Korean pine.

Picea koraiensis (Korean spruce) is a boreal tree species of considerable ecological and economic importance in Northeast Asia, particularly within its natural range in China, Russia and the Korean Peninsula. This study investigated spatial variation in key bioclimatic factors and their relationships with terrain gradients to clarify how these variables influence the species’ distribution. Using high-resolution climate data and species-occurrence records, we examined the effects of elevation, slope and aspect on temperature and precipitation patterns across the species’ range. Precipitation-related variables, including bio13, bio14 and bio15, were closely associated with habitat suitability, whereas elevation and slope affected local temperature stability and moisture availability. Higher elevations and steeper slopes generally provided cooler, wetter conditions favorable for P. koraiensis. Climate modelling predicts that suitable habitats will shift northwards and to higher elevations in response to climate change and are likely to contract under high-emission scenarios. Identifying climate refugia and areas with stable bioclimatic conditions provides important insights for conservation prioritization. This study highlights the need for targeted conservation strategies, including protecting identified climate refugia, maintaining or restoring connectivity corridors between suitable habitats, and carefully assessing assisted migration to climatically favorable northern and higher-elevation areas, to support the long-term viability of the species under rapid climate change.