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The aim of this work was to assess the regeneration capacity of Amelanchier alnifolia var. cusickii and Lonicera kamtschatica cv. ‘Jugana’ from different types of explants under various hormonal treatments. The whole leaves, petioles, and internodal segments of in vitro plants were examined as explants. Several plant growth regulators (cytokinins and auxins) were evaluated for their ability to induce adventitious regeneration. Direct and indirect organogenesis was achieved under certain culture conditions in both species. The frequency of shoot regeneration was strongly dependent on concentrations of plant growth regulators in the induction media (L.kamtschatica ‘Jugana’) or concentrations of plant growth regulators in the induction media and type of explant (A. alnifolia var. cusickii). Results showed that leaves were not suitable explants for A. alnifolia var. cusickii. Both species were able to regenerate shoots from internodal segments and petioles. The highest induction of shoots was obtained on Murashige and Skoog (MS) medium enriched with 2 mg/L thidiazuron (TDZ) and 0.5 mg/L indole-3-butyric acid (IBA) for Amelanchier alnifolia and with 1 mg/L TDZ and 0.2 mg/L indole-3-acetic acid (IAA) for L.kamtschatica ‘Jugana’. Obtained adventitious shoots were further proliferated in order to investigate their multiplication capacity. The multiplication of shoots was successful in all cultivars, with the best results reported in A. alnifolia var. cusickii (7.07 shoots/explant on average).
Júlia Hunková; Monika Szabóová; Alena Gajdošová. Protocols for Adventitious Regeneration of Amelanchier alnifolia var. cusickii and Lonicera kamtschatica ‘Jugana’. Plants 2021, 10, 1155 .
AMA StyleJúlia Hunková, Monika Szabóová, Alena Gajdošová. Protocols for Adventitious Regeneration of Amelanchier alnifolia var. cusickii and Lonicera kamtschatica ‘Jugana’. Plants. 2021; 10 (6):1155.
Chicago/Turabian StyleJúlia Hunková; Monika Szabóová; Alena Gajdošová. 2021. "Protocols for Adventitious Regeneration of Amelanchier alnifolia var. cusickii and Lonicera kamtschatica ‘Jugana’." Plants 10, no. 6: 1155.
Berry fruit species are, in many countries, considered biologically and economically valuable and important species of small fruits. The aim of this work was to examine the influence of either decreased or increased mesos concentrations (MgSO4, CaCl2, and KH2PO4) on shoot multiplication of five cultivars of three small fruit species (Amelanchier alnifolia var. cusickii, Rubus fruticosus ‘Black Satin’ and ‘Loch Ness’, and Vaccinium corymbosum ‘Brigitta Blue’ and ‘Toro’). Mesos nutrients were manipulated from half to four times their base concentration. The results indicate that mesos manipulation significantly influences the number and length of shoots in most of the studied cultivars. The greatest multiplication rate for A. alnifolia was achieved with tripled mesos, whereas ‘Black Satin’ and ‘Loch Ness’ reacted positively to a lower (1–2x) concentration of mesos. Decreasing the concentration of mesos to half led to worse quality in both blackberry and Saskatoon shoots. ‘Brigitta Blue’ was more sensitive to greater mesos concentrations compared to ‘Toro’. Optimizing the mineral nutrition of plants cultivated in vitro enhances their multiplication rate and contributes to a higher production of good quality plantlets.
Júlia Hunková; Alena Gajdošová; Monika Szabóová. Effect of Mesos Components (MgSO4, CaCl2, KH2PO4) on In Vitro Shoot Growth of Blackberry, Blueberry, and Saskatoon. Plants 2020, 9, 935 .
AMA StyleJúlia Hunková, Alena Gajdošová, Monika Szabóová. Effect of Mesos Components (MgSO4, CaCl2, KH2PO4) on In Vitro Shoot Growth of Blackberry, Blueberry, and Saskatoon. Plants. 2020; 9 (8):935.
Chicago/Turabian StyleJúlia Hunková; Alena Gajdošová; Monika Szabóová. 2020. "Effect of Mesos Components (MgSO4, CaCl2, KH2PO4) on In Vitro Shoot Growth of Blackberry, Blueberry, and Saskatoon." Plants 9, no. 8: 935.
Júlia Hunková; Alena Gajdošová. In vitro rooting and acclimatization of Amelanchier alnifolia (Nutt.) Nutt. ex M. Roem: Testing of auxin, spermidine, and gibberellin for overcoming dormancy. Journal of Berry Research 2019, 9, 549 -561.
AMA StyleJúlia Hunková, Alena Gajdošová. In vitro rooting and acclimatization of Amelanchier alnifolia (Nutt.) Nutt. ex M. Roem: Testing of auxin, spermidine, and gibberellin for overcoming dormancy. Journal of Berry Research. 2019; 9 (3):549-561.
Chicago/Turabian StyleJúlia Hunková; Alena Gajdošová. 2019. "In vitro rooting and acclimatization of Amelanchier alnifolia (Nutt.) Nutt. ex M. Roem: Testing of auxin, spermidine, and gibberellin for overcoming dormancy." Journal of Berry Research 9, no. 3: 549-561.
The aim of this work was to evaluate shoot multiplication of two blackberry (Rubus fruticosus L.) cultivars ?Black Satin? and ?Loch Ness? on two culture media: Murashige & Skoog (MS) and its modification Murashige & Skoog Van der Salm medium (MS VDS) which differ only in iron source (FeNaEDTA, FeEDDHA, respectively). Statistical analyses showed no significant difference in shoot multiplication between different iron sources for both tested cultivars. For ?Black Satin? it was shown that double concentration of chelates FeNaEDTA and FeEDDHA in culture media negatively affected on shoot growth and multiplication. These results can be very useful for further optimization of micropropagation process for different Rubus cultivars.
Júlia Hunková; Gabriela Libiaková; Jozef Fejér; Tatjana Vujovic; Alena Gajdošová. Testing of different iron sources and concentrations on shoot multiplication of blackberry (Rubus fruticosus L.). Genetika 2018, 50, 351 -356.
AMA StyleJúlia Hunková, Gabriela Libiaková, Jozef Fejér, Tatjana Vujovic, Alena Gajdošová. Testing of different iron sources and concentrations on shoot multiplication of blackberry (Rubus fruticosus L.). Genetika. 2018; 50 (1):351-356.
Chicago/Turabian StyleJúlia Hunková; Gabriela Libiaková; Jozef Fejér; Tatjana Vujovic; Alena Gajdošová. 2018. "Testing of different iron sources and concentrations on shoot multiplication of blackberry (Rubus fruticosus L.)." Genetika 50, no. 1: 351-356.
The aim of this work was to develop protocols for micropropagation of selected Rubus cultivars – ‘Tulameen’ and ‘Black Jewel’ (Rubus idaeus L.), ‘Bl...
Júlia Hunková; Gabriela Libiaková; Alena Gajdošová. Shoot proliferation ability of selected cultivars of Rubus spp. as influenced by genotype and cytokinin concentration. Journal of Central European Agriculture 2016, 17, 379 -390.
AMA StyleJúlia Hunková, Gabriela Libiaková, Alena Gajdošová. Shoot proliferation ability of selected cultivars of Rubus spp. as influenced by genotype and cytokinin concentration. Journal of Central European Agriculture. 2016; 17 (2):379-390.
Chicago/Turabian StyleJúlia Hunková; Gabriela Libiaková; Alena Gajdošová. 2016. "Shoot proliferation ability of selected cultivars of Rubus spp. as influenced by genotype and cytokinin concentration." Journal of Central European Agriculture 17, no. 2: 379-390.
PB1-F2 protein of influenza A virus (IAV) was cloned in a plum pox virus (PPV) genome-based vector and attempts to express it in biolistically transfected Nicotiana benthamiana plants were performed. The vector-insert construct replicated in infected plants properly and was stable during repeated passage by mechanical inoculation, as demonstrated by disease symptoms and immunoblot detection of PPV capsid protein, while PB1-F2-specific band was more faint. We showed that it was due its low solubility. Modification of sample preparation (denaturation/solubilization preceding the centrifugation of cell debris) led to substantial signal enhancement. Maximal level of PB1-F2 expression in plants was observed 12 days post inoculation (dpi). Only 1% SDS properly solubilized the protein, other detergents were much less efficient. Solubilization with 8M urea released approximately 50% of PB1-F2 from the plant tissues, thus the treatment with this removable chaotropic agent may be a good starting point for the purification of the protein for eventual functional studies in the future.
M. Kamencayová; I. Košík; Júlia Hunková; Zdeno Subr. Transient expression of the influenza A virus PB1-F2 protein using a plum pox virus-based vector in Nicotiana benthamiana. Acta virologica 2014, 58, 274 -277.
AMA StyleM. Kamencayová, I. Košík, Júlia Hunková, Zdeno Subr. Transient expression of the influenza A virus PB1-F2 protein using a plum pox virus-based vector in Nicotiana benthamiana. Acta virologica. 2014; 58 (3):274-277.
Chicago/Turabian StyleM. Kamencayová; I. Košík; Júlia Hunková; Zdeno Subr. 2014. "Transient expression of the influenza A virus PB1-F2 protein using a plum pox virus-based vector in Nicotiana benthamiana." Acta virologica 58, no. 3: 274-277.