We report a facile strategy to prepare chitosan (CS) hydrogels that eliminates the need for chemical crosslinking for advanced biomedical therapies. This approach gives controlled properties to the hydrogels by incorporating a natural bioactive phenolic compound, phloroglucinol (PG), into their microstructure. The adsorption of PG onto CS chains enhanced the hydrogels' antioxidant activity by up to 25 % and resulted in a denser, more entangled structure, reducing the pore size by 59 μm while maintaining porosity above 94 %. This allowed us to finely adjust pore size and swelling capacity. These structural properties make these hydrogels well-suited for wound healing dressings, promoting fibroblast proliferation and exhibiting excellent hemocompatibility. Furthermore, to ensure the versatility of these hydrogels, herein, we demonstrate their potential as drug delivery systems, particularly for dermal infections. The drug release can be controlled by a combination of drug diffusion through the swollen hydrogel and relaxation of the CS chains. In summary, our hydrogels leverage the synergistic effects of CS's antibacterial and antifungal properties with PG's antimicrobial and anti-inflammatory attributes, positioning them as promising candidates for biomedical and pharmaceutical applications, more specifically in advanced wound healing therapies with local drug delivery.

In this study, a potential hard tissue substitute was mimicked using collagen/mangosteen porous scaffolds. Collagen was extracted from Tilapia fish skin and mangosteen from the waste peel of the respective fruit. Sodium trimetaphosphate was used for the phosphorylation of these scaffolds to improve the nucleation sites for the mineralization process. Phosphate groups were incorporated in the collagen structure as confirmed by their attenuated total reflection Fourier transform infrared (ATR-FTIR) bands. The phosphorylation and mangosteen addition increased the thermal stability of the collagen triple helix structure, as demonstrated by differential scanning calorimetry (DSC) and thermogravimetry (TGA) characterizations. Mineralization was successfully achieved, and the presence of calcium phosphate was visualized by scanning electron microscopy (SEM). Nevertheless, the porous structure was maintained, which is an essential characteristic for the desired application. The deposited mineral was amorphous calcium phosphate, as confirmed by energy dispersive X-ray spectroscopy (EDX) results.