In this study, a potential hard tissue substitute was mimicked using collagen/mangosteen porous scaffolds. Collagen was extracted from Tilapia fish skin and mangosteen from the waste peel of the respective fruit. Sodium trimetaphosphate was used for the phosphorylation of these scaffolds to improve the nucleation sites for the mineralization process. Phosphate groups were incorporated in the collagen structure as confirmed by their attenuated total reflection Fourier transform infrared (ATR-FTIR) bands. The phosphorylation and mangosteen addition increased the thermal stability of the collagen triple helix structure, as demonstrated by differential scanning calorimetry (DSC) and thermogravimetry (TGA) characterizations. Mineralization was successfully achieved, and the presence of calcium phosphate was visualized by scanning electron microscopy (SEM). Nevertheless, the porous structure was maintained, which is an essential characteristic for the desired application. The deposited mineral was amorphous calcium phosphate, as confirmed by energy dispersive X-ray spectroscopy (EDX) results.

Radiation therapy (RT) is crucial for cancer treatment but often causes severe side effects that impact quality of life, emphasizing the need for better tissue radioprotection strategies. This study investigated the radioprotective potential of red LED irradiation using zebrafish embryos. After determining the median lethal dose of gamma radiation $(\mathbf{LD}_{\mathbf{50}})$ to be 16.7 Gy, embryos were exposed to red LED before gamma radiation. While red LED alone did not affect survival rates, pre-treatment with red light increased survival to over 50% after LD 50 gamma radiation. These results suggest that red LED could help reduce the acute effects of RT.