Toxicity of the C₆₀ fullerene aqueous colloid solution in vitro and in vivo experiments was studied. C₆₀ fullerene in the concentration range of 3.6-144 µg/mL demonstrated low toxicity against human embryonic kidney (HEK293) cells with 383.4 μg/mL IC₅₀ value. The acute toxicity of C₆₀ fullerene in a dose progression (75, 150, 300, 600, 1200 and 1800 mg/kg) was investigated at 14^th day after single intraperitoneal (i.p.) administration into mice. No toxic effect of С₆₀ fullerene in the dose range of 75-150 mg/kg was observed; the calculated LD₅₀ value was 721 mg/kg. The toxic effect of C₆₀ fullerene was observed at a concentration of 300 mg/kg and higher, it was accompanied with mice behavior disturbance, hematotoxicity and pathomorphological changes in spleen, hepatic and kidney tissues. In mice treated with 600 mg/kg of C₆₀ fullerene indices of inflammatory process were detected, whereas at 1200 mg/kg dose leukopoies and thrombocytopoies were found to be suppressed. Thus, C₆₀ fullerene aqueous colloid solution in a dose range of 75-150 mg/kg is nontoxic and could be used for biomedical purposes.

The effects of C₆₀FAS (50 and 500 μg/kg) supplementation, in a normal physiological state and after restraint stress exposure, on prooxidant/antioxidant balance in rat tissues were explored and compared with the effects of the known exogenous antioxidant N‐acetylcysteine. Oxidative stress biomarkers (ROS, O₂⋅⁻, H₂O₂, and lipid peroxidation) and indices of antioxidant status (MnSOD, catalase, GPx, GST, γ‐GCL, GR activities, and GSH level) were measured in the brain and the heart. In addition, protein expression of Nrf2 in the nuclear and cytosol fractions as well as the protein level of antiradical enzyme MnSOD and GSH‐related enzymes γ‐GCLC, GPx, and GSTP as downstream targets of Nrf2 was evaluated by western blot analysis. Under a stress condition, C₆₀FAS attenuates ROS generation and O₂⋅⁻ and H₂O₂ releases and thus decreases lipid peroxidation as well as increases rat tissue antioxidant capacity. We have shown that C₆₀FAS supplementation has dose‐dependent and tissue‐specific effects. C₆₀FAS strengthened the antiradical defense through the upregulation of MnSOD in brain cells and maintained MnSOD protein content at the control level in the myocardium. Moreover, C₆₀FAS enhanced the GSH level and the activity/protein expression of GSH‐related enzymes. Correlation of these changes with Nrf2 protein content suggests that under stress exposure, along with other mechanisms, the Nrf2/ARE‐antioxidant pathway may be involved in regulation of glutathione homeostasis. In our study, in an in vivo model, when C₆₀FAS (50 and 500 μg/kg) was applied alone, no significant changes in Nrf2 protein expression as well as in activity/protein levels of MnSOD and GSH‐related enzymes in both tissues types were observed. All these facts allow us to assume that in the in vivo model, C₆₀FAS affects on the brain and heart endogenous antioxidative statuses only during the oxidative stress condition.

Fullerene C60 as a representative of carbon nanocompounds is suggested to be promising agent for application in photodynamic therapy due to its unique phys

The main aim of this work was to evaluate the effect of doxorubicin in complex with C60 fullerene (C60 + Dox) on the growth and metastasis of L

Growth experiments of transplanted malignant tumors in the presence of water-soluble C60 fullerenes were performed on groups of mice. It was found that C60 fullerenes efficiently inhibit the growth of transplanted malignant tumors. This behavior can be explained through their high antioxidant activity and the blocking of the specific cell receptors (for example, endothelial growth factor). The findings demonstrate the possibility of using C60 fullerenes in anticancer therapy.