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Listeria spp. is a diverse genus of Gram-positive bacteria commonly present in the environment while L. monocytogenes and L. ivanovii are well known human and ruminant pathogens. The aim of the present study was to reveal the prevalence and genetic diversity of L. monocytogenes and other Listeria spp. and to identify the factors related to the abundance of pathogen at cattle farms. A total of 521 animal and environmental samples from 27 meat and dairy cattle farms were investigated and the genetic diversity of L. monocytogenes isolates was studied with WGS. The prevalence of Listeria was 58.9%, while of L. monocytogenes it was −11%. The highest prevalence of L. monocytogenes was found in the environment—soil samples near to manure storage (93%), mixed feed from the feeding trough and hay (29%), water samples from farms drinking trough (28%) and cattle feces (28%). Clonal complexes (CC) of CC37 (30%), CC11 (20%) and CC18 (17%) (all IIa serogroup) were predominant L. monocytogenes clones. CC18, CC37 and CC8 were isolated from case farms and CC37, CC11 and CC18 from farms without listeriosis history. Only one hypervirulent CC4 (1%) was isolated from the case farm. Sequence types (STs) were not associated with the isolation source, except for ST7, which was significantly associated with soil (p< 0.05). The contamination of soil, feeding tables and troughs with L. monocytogenes was associated with an increased prevalence of L. monocytogenes at farms. Our study indicates the importance of hygienic practice in the prevention of the dissemination of L. monocytogenes in the cattle farm environment.
Margarita Terentjeva; Žanete Šteingolde; Irēna Meistere; Didzis Elferts; Jeļena Avsejenko; Madara Streikiša; Silva Gradovska; Laura Alksne; Juris Ķibilds; Aivars Bērziņš. Prevalence, Genetic Diversity and Factors Associated with Distribution of Listeria monocytogenes and Other Listeria spp. in Cattle Farms in Latvia. Pathogens 2021, 10, 851 .
AMA StyleMargarita Terentjeva, Žanete Šteingolde, Irēna Meistere, Didzis Elferts, Jeļena Avsejenko, Madara Streikiša, Silva Gradovska, Laura Alksne, Juris Ķibilds, Aivars Bērziņš. Prevalence, Genetic Diversity and Factors Associated with Distribution of Listeria monocytogenes and Other Listeria spp. in Cattle Farms in Latvia. Pathogens. 2021; 10 (7):851.
Chicago/Turabian StyleMargarita Terentjeva; Žanete Šteingolde; Irēna Meistere; Didzis Elferts; Jeļena Avsejenko; Madara Streikiša; Silva Gradovska; Laura Alksne; Juris Ķibilds; Aivars Bērziņš. 2021. "Prevalence, Genetic Diversity and Factors Associated with Distribution of Listeria monocytogenes and Other Listeria spp. in Cattle Farms in Latvia." Pathogens 10, no. 7: 851.
The rapid development of megacities, and their growing connectedness across the world is becoming a distinct driver for emerging disease outbreaks. Early detection of unusual disease emergence and spread should therefore include such cities as part of risk-based surveillance. A catch-all metagenomic sequencing approach of urban sewage could potentially provide an unbiased insight into the dynamics of viral pathogens circulating in a community irrespective of access to care, a potential which already has been proven for the surveillance of poliovirus. Here, we present a detailed characterization of sewage viromes from a snapshot of 81 high density urban areas across the globe, including in-depth assessment of potential biases, as a proof of concept for catch-all viral pathogen surveillance. We show the ability to detect a wide range of viruses and geographical and seasonal differences for specific viral groups. Our findings offer a cross-sectional baseline for further research in viral surveillance from urban sewage samples and place previous studies in a global perspective.
David F. Nieuwenhuijse; Bas B. Oude Munnink; My V. T. Phan; Rene S. Hendriksen; Artan Bego; Catherine Rees; Elizabeth Heather Neilson; Kris Coventry; Peter Collignon; Franz Allerberger; Teddie O. Rahube; Guilherme Oliveira; Ivan Ivanov; Thet Sopheak; Yith Vuthy; Christopher K. Yost; Djim-Adjim Tabo; Sara Cuadros-Orellana; Changwen Ke; Huanying Zheng; Li Baisheng; Xiaoyang Jiao; Pilar Donado-Godoy; Kalpy Julien Coulibaly; Jasna Hrenovic; Matijana Jergović; Renáta Karpíšková; Bodil Elsborg; Mengistu Legesse; Tadesse Eguale; Annamari Heikinheimo; Jose Eduardo Villacis; Bakary Sanneh; Lile Malania; Andreas Nitsche; Annika Brinkmann; Courage Kosi Setsoafia Saba; Bela Kocsis; Norbert Solymosi; Thorunn R. Thorsteinsdottir; Abdulla Mohamed Hatha; Masoud Alebouyeh; Dearbhaile Morris; Louise O’Connor; Martin Cormican; Jacob Moran-Gilad; Antonio Battisti; Patricia Alba; Zeinegul Shakenova; Ciira Kiiyukia; Eric Ng’Eno; Lul Raka; Aivars Bērziņš; Jeļena Avsejenko; Vadims Bartkevics; Christian Penny; Heraa Rajandas; Sivachandran Parimannan; Malcolm Vella Haber; Pushkar Pal; Heike Schmitt; Mark van Passel; Milou G.M. van de Schans; Tina Zuidema; Gert-Jan Jeunen; Neil Gemmell; Kayode Fashae; Astrid Louise Wester; Rune Holmstad; Rumina Hasan; Sadia Shakoor; Maria Luz Zamudio Rojas; Dariusz Wasyl; Golubinka Bosevska; Mihail Kochubovski; Cojocaru Radu; Amy Gassama†; Vladimir Radosavljevic; Moon Y.F. Tay; Rogelio Zuniga-Montanez; Stefan Wuertz; Dagmar Gavačová; Marija Trkov; Karen Keddy; Kerneels Esterhuyse; Marta Cerdà-Cuéllar; Sujatha Pathirage; D.G.Joakim Larsson; Leif Norrgren; Stefan Örn; Tanja Van der Heijden; Happiness Houka Kumburu; Ana Maria de RodaHusman; Berthe-Marie Njanpop-Lafourcade; Pawou Bidjada; Somtinda Christelle Nikiema-Pessinaba; Belkis Levent; John Scott Meschke; Nicola Koren Beck; Chinh Van Dang; Doan Minh Nguyen Tran; Nguyen Do Phuc; Geoffrey Kwenda; Patrick Munk; Shweta Venkatakrishnan; Frank M. Aarestrup; Matthew Cotten; Marion P. G. Koopmans; the Global Sewage Surveillance project consortium. Setting a baseline for global urban virome surveillance in sewage. Scientific Reports 2020, 10, 1 -13.
AMA StyleDavid F. Nieuwenhuijse, Bas B. Oude Munnink, My V. T. Phan, Rene S. Hendriksen, Artan Bego, Catherine Rees, Elizabeth Heather Neilson, Kris Coventry, Peter Collignon, Franz Allerberger, Teddie O. Rahube, Guilherme Oliveira, Ivan Ivanov, Thet Sopheak, Yith Vuthy, Christopher K. Yost, Djim-Adjim Tabo, Sara Cuadros-Orellana, Changwen Ke, Huanying Zheng, Li Baisheng, Xiaoyang Jiao, Pilar Donado-Godoy, Kalpy Julien Coulibaly, Jasna Hrenovic, Matijana Jergović, Renáta Karpíšková, Bodil Elsborg, Mengistu Legesse, Tadesse Eguale, Annamari Heikinheimo, Jose Eduardo Villacis, Bakary Sanneh, Lile Malania, Andreas Nitsche, Annika Brinkmann, Courage Kosi Setsoafia Saba, Bela Kocsis, Norbert Solymosi, Thorunn R. Thorsteinsdottir, Abdulla Mohamed Hatha, Masoud Alebouyeh, Dearbhaile Morris, Louise O’Connor, Martin Cormican, Jacob Moran-Gilad, Antonio Battisti, Patricia Alba, Zeinegul Shakenova, Ciira Kiiyukia, Eric Ng’Eno, Lul Raka, Aivars Bērziņš, Jeļena Avsejenko, Vadims Bartkevics, Christian Penny, Heraa Rajandas, Sivachandran Parimannan, Malcolm Vella Haber, Pushkar Pal, Heike Schmitt, Mark van Passel, Milou G.M. van de Schans, Tina Zuidema, Gert-Jan Jeunen, Neil Gemmell, Kayode Fashae, Astrid Louise Wester, Rune Holmstad, Rumina Hasan, Sadia Shakoor, Maria Luz Zamudio Rojas, Dariusz Wasyl, Golubinka Bosevska, Mihail Kochubovski, Cojocaru Radu, Amy Gassama†, Vladimir Radosavljevic, Moon Y.F. Tay, Rogelio Zuniga-Montanez, Stefan Wuertz, Dagmar Gavačová, Marija Trkov, Karen Keddy, Kerneels Esterhuyse, Marta Cerdà-Cuéllar, Sujatha Pathirage, D.G.Joakim Larsson, Leif Norrgren, Stefan Örn, Tanja Van der Heijden, Happiness Houka Kumburu, Ana Maria de RodaHusman, Berthe-Marie Njanpop-Lafourcade, Pawou Bidjada, Somtinda Christelle Nikiema-Pessinaba, Belkis Levent, John Scott Meschke, Nicola Koren Beck, Chinh Van Dang, Doan Minh Nguyen Tran, Nguyen Do Phuc, Geoffrey Kwenda, Patrick Munk, Shweta Venkatakrishnan, Frank M. Aarestrup, Matthew Cotten, Marion P. G. Koopmans, the Global Sewage Surveillance project consortium. Setting a baseline for global urban virome surveillance in sewage. Scientific Reports. 2020; 10 (1):1-13.
Chicago/Turabian StyleDavid F. Nieuwenhuijse; Bas B. Oude Munnink; My V. T. Phan; Rene S. Hendriksen; Artan Bego; Catherine Rees; Elizabeth Heather Neilson; Kris Coventry; Peter Collignon; Franz Allerberger; Teddie O. Rahube; Guilherme Oliveira; Ivan Ivanov; Thet Sopheak; Yith Vuthy; Christopher K. Yost; Djim-Adjim Tabo; Sara Cuadros-Orellana; Changwen Ke; Huanying Zheng; Li Baisheng; Xiaoyang Jiao; Pilar Donado-Godoy; Kalpy Julien Coulibaly; Jasna Hrenovic; Matijana Jergović; Renáta Karpíšková; Bodil Elsborg; Mengistu Legesse; Tadesse Eguale; Annamari Heikinheimo; Jose Eduardo Villacis; Bakary Sanneh; Lile Malania; Andreas Nitsche; Annika Brinkmann; Courage Kosi Setsoafia Saba; Bela Kocsis; Norbert Solymosi; Thorunn R. Thorsteinsdottir; Abdulla Mohamed Hatha; Masoud Alebouyeh; Dearbhaile Morris; Louise O’Connor; Martin Cormican; Jacob Moran-Gilad; Antonio Battisti; Patricia Alba; Zeinegul Shakenova; Ciira Kiiyukia; Eric Ng’Eno; Lul Raka; Aivars Bērziņš; Jeļena Avsejenko; Vadims Bartkevics; Christian Penny; Heraa Rajandas; Sivachandran Parimannan; Malcolm Vella Haber; Pushkar Pal; Heike Schmitt; Mark van Passel; Milou G.M. van de Schans; Tina Zuidema; Gert-Jan Jeunen; Neil Gemmell; Kayode Fashae; Astrid Louise Wester; Rune Holmstad; Rumina Hasan; Sadia Shakoor; Maria Luz Zamudio Rojas; Dariusz Wasyl; Golubinka Bosevska; Mihail Kochubovski; Cojocaru Radu; Amy Gassama†; Vladimir Radosavljevic; Moon Y.F. Tay; Rogelio Zuniga-Montanez; Stefan Wuertz; Dagmar Gavačová; Marija Trkov; Karen Keddy; Kerneels Esterhuyse; Marta Cerdà-Cuéllar; Sujatha Pathirage; D.G.Joakim Larsson; Leif Norrgren; Stefan Örn; Tanja Van der Heijden; Happiness Houka Kumburu; Ana Maria de RodaHusman; Berthe-Marie Njanpop-Lafourcade; Pawou Bidjada; Somtinda Christelle Nikiema-Pessinaba; Belkis Levent; John Scott Meschke; Nicola Koren Beck; Chinh Van Dang; Doan Minh Nguyen Tran; Nguyen Do Phuc; Geoffrey Kwenda; Patrick Munk; Shweta Venkatakrishnan; Frank M. Aarestrup; Matthew Cotten; Marion P. G. Koopmans; the Global Sewage Surveillance project consortium. 2020. "Setting a baseline for global urban virome surveillance in sewage." Scientific Reports 10, no. 1: 1-13.
African swine fever (ASF) was first detected in Latvia in wild boar at the Eastern border in June 2014. Since then ASF has continued to spread in wild boar populations covering almost whole territory of the country. Sporadic outbreaks occurred at the same time in domestic pig holdings located in wild boar infected areas. Here we present the results of the epidemiological investigation in two large commercial farms. Several parameters were analyzed to determine the high risk period (HRP) and to investigate the ASF virus spread within the farm. Clinical data, mortality rates and laboratory results proved to be good indicators for estimating the HRP. The measures for early disease detection, particularly the enhanced passive surveillance that is targeting dead and sick pigs, were analyzed and discussed. Enhanced passive surveillance proved to be a key element to detect ASF at an early stage. The study also showed that ASF virus might spread slowly within a large farm depending mainly on direct contacts between pigs and the level of internal biosecurity. Findings suggest improvements in outbreak prevention, control measures and may contribute to a better understanding of ASF spreading patterns within large pig herds. Culling of all pigs in large commercial farms could be reconsidered under certain conditions.
Kristīne Lamberga; Edvīns Oļševskis; Mārtiņš Seržants; Aivars Bērziņš; Arvo Viltrop; Klaus Depner. African Swine Fever in Two Large Commercial Pig Farms in LATVIA—Estimation of the High Risk Period and Virus Spread within the Farm. Veterinary Sciences 2020, 7, 105 .
AMA StyleKristīne Lamberga, Edvīns Oļševskis, Mārtiņš Seržants, Aivars Bērziņš, Arvo Viltrop, Klaus Depner. African Swine Fever in Two Large Commercial Pig Farms in LATVIA—Estimation of the High Risk Period and Virus Spread within the Farm. Veterinary Sciences. 2020; 7 (3):105.
Chicago/Turabian StyleKristīne Lamberga; Edvīns Oļševskis; Mārtiņš Seržants; Aivars Bērziņš; Arvo Viltrop; Klaus Depner. 2020. "African Swine Fever in Two Large Commercial Pig Farms in LATVIA—Estimation of the High Risk Period and Virus Spread within the Farm." Veterinary Sciences 7, no. 3: 105.
I. Piginka-Vjaceslavova; L. Ansonska; Z. Steingolde; Aivars Bērziņš; J. Avsejenko. Listeria Species Causing Abortions in Cattle and Lesions in Aborted Fetuses. Journal of Comparative Pathology 2020, 174, 192 .
AMA StyleI. Piginka-Vjaceslavova, L. Ansonska, Z. Steingolde, Aivars Bērziņš, J. Avsejenko. Listeria Species Causing Abortions in Cattle and Lesions in Aborted Fetuses. Journal of Comparative Pathology. 2020; 174 ():192.
Chicago/Turabian StyleI. Piginka-Vjaceslavova; L. Ansonska; Z. Steingolde; Aivars Bērziņš; J. Avsejenko. 2020. "Listeria Species Causing Abortions in Cattle and Lesions in Aborted Fetuses." Journal of Comparative Pathology 174, no. : 192.
Background and Objectives: Legionella is one of the most important water-related pathogens. Inside the water supply systems and the biofilms, Legionella interact with other bacteria and free-living amoeba (FLA). Several amoebas may serve as hosts for bacteria in aquatic systems. This study aimed to investigate the co-occurrence of Legionella spp. and FLA in drinking water supply systems. Materials and Methods: A total of 268 water samples were collected from apartment buildings, hotels, and public buildings. Detection of Legionella spp. was performed in accordance with ISO 11731:2017 standard. Three different polymerase chain reaction (PCR) protocols were used to identify FLA. Results: Occurrence of Legionella varied from an average of 12.5% in cold water samples with the most frequent occurrence observed in hot water, in areas receiving untreated groundwater, where 54.0% of the samples were Legionella positive. The occurrence of FLA was significantly higher. On average, 77.2% of samples contained at least one genus of FLA and, depending on the type of sample, the occurrence of FLA could reach 95%. In the samples collected during the study, Legionella was always isolated along with FLA, no samples containing Legionella in the absence of FLA were observed. Conclusions: The data obtained in our study can help to focus on the extensive distribution, close interaction, and long-term persistence of Legionella and FLA. Lack of Legionella risk management plans and control procedures may promote further spread of Legionella in water supply systems. In addition, the high incidence of Legionella-related FLA suggests that traditional monitoring methods may not be sufficient for Legionella control.
Olga Valciņa; Daina Pūle; Artjoms Mališevs; Jūlija Trofimova; Svetlana Makarova; Genadijs Konvisers; Aivars Bērziņš; Angelika Krūmiņa. Co-Occurrence of Free-Living Amoeba and Legionella in Drinking Water Supply Systems. Medicina 2019, 55, 492 .
AMA StyleOlga Valciņa, Daina Pūle, Artjoms Mališevs, Jūlija Trofimova, Svetlana Makarova, Genadijs Konvisers, Aivars Bērziņš, Angelika Krūmiņa. Co-Occurrence of Free-Living Amoeba and Legionella in Drinking Water Supply Systems. Medicina. 2019; 55 (8):492.
Chicago/Turabian StyleOlga Valciņa; Daina Pūle; Artjoms Mališevs; Jūlija Trofimova; Svetlana Makarova; Genadijs Konvisers; Aivars Bērziņš; Angelika Krūmiņa. 2019. "Co-Occurrence of Free-Living Amoeba and Legionella in Drinking Water Supply Systems." Medicina 55, no. 8: 492.
Background Campylobacter is the main cause of bacterial gastroenteritis worldwide. The main transmission route is through consumption of food contaminated with Campylobacter species or contact with infected animals. In Latvia, the prevalence of campylobacteriosis is reported to be low (4.6 cases per 100,000 population in 2016). Aim To determine prevalence, species spectrum and antimicrobial resistance (AMR) of Campylobacter spp. in Latvia, using data from various livestock and human clinical samples. Methods We analysed data of Campylobacter microbiological monitoring and AMR (2008 and 2014–16) in Latvia. Data from broilers, poultry, pigs, calves and humans were used to determine prevalence of Campylobacter. Additionally, 45 different origin isolates (22 human) were sequenced on the Illumina MiSeq platform; for each isolate core genome multilocus sequence typing was used and relevant antimicrobial resistance mechanisms were identified. Results Overall, Campylobacter prevalence in was 83.3% in pigs, 50.2% in broilers, 16.1% in calves and 5.3% in humans; C. jejuni was the predominant species in all sources except pigs where C. coli was main species. High level of resistance in Campylobacter were observed against fluoroquinolones, tetracycline and streptomycin, in most of sequenced isolates genetic determinants of relevant AMR profiles were identified. Conclusions In Latvia, prevalence of Campylobacter in livestock is high, especially in pigs and broilers; prevalence in poultry and humans were lower than in other European countries. AMR analysis reveals increase of streptomycin and tetracycline resistant broiler origin C. jejuni strains. WGS demonstrates a high compliance between resistance phenotype and genotype for quinolones and tetracyclines.
Irēna Meistere; Juris Ķibilds; Lāsma Eglīte; Laura Alksne; Jeļena Avsejenko; Alla Cibrovska; Svetlana Makarova; Madara Streikiša; Lelde Grantiņa-Ieviņa; Aivars Bērziņš. Campylobacter species prevalence, characterisation of antimicrobial resistance and analysis of whole-genome sequence of isolates from livestock and humans, Latvia, 2008 to 2016. Eurosurveillance 2019, 24, 1 .
AMA StyleIrēna Meistere, Juris Ķibilds, Lāsma Eglīte, Laura Alksne, Jeļena Avsejenko, Alla Cibrovska, Svetlana Makarova, Madara Streikiša, Lelde Grantiņa-Ieviņa, Aivars Bērziņš. Campylobacter species prevalence, characterisation of antimicrobial resistance and analysis of whole-genome sequence of isolates from livestock and humans, Latvia, 2008 to 2016. Eurosurveillance. 2019; 24 (31):1.
Chicago/Turabian StyleIrēna Meistere; Juris Ķibilds; Lāsma Eglīte; Laura Alksne; Jeļena Avsejenko; Alla Cibrovska; Svetlana Makarova; Madara Streikiša; Lelde Grantiņa-Ieviņa; Aivars Bērziņš. 2019. "Campylobacter species prevalence, characterisation of antimicrobial resistance and analysis of whole-genome sequence of isolates from livestock and humans, Latvia, 2008 to 2016." Eurosurveillance 24, no. 31: 1.
The aim of the present study was to detect the prevalence and antimicrobial resistance of fecal indicators and major foodborne pathogens in feces of calves and to identify the factors associated with increased prevalence of resistant bacteria on farms. Altogether, 180 rectal swabs were collected from 18 farms in Latvia. Samples were investigated to detect the prevalence and antimicrobial resistance of Escherichia coli, Enterococcus spp., Listeria monocytogenes, Yersinia enterocolitica, Salmonella spp., Staphylococcus aureus, and Campylobacter spp. Among all, 64% (74/110) of commensal E. coli, 100% (78/78) Enterococcus faecalis and 96% (22/23) Enterococcus faecium isolates were resistant at least to one antibiotic. The prevalence of extended-spectrum β-lactamase (ESBL)/AmpC-producing E. coli were 11.1% (20/180) with blaCTX-M, blaTEM, and blaCMY genes identified. Campylobacter jejuni (12.8%, 23/180) and Campylobacter coli (2.8%, 5/180) were the most resistant to tetracycline (61%, 14/23; 100%, 5/5) and fluoroquinolones (61%, 14/23; 100%, 5/5). Prevalence of L. monocytogenes was 0.6% (1/180) and S. aureus 1.7% (3/180). All samples were Salmonella and Y. enterocolitica negative. Farm size, bought calves, contact with other calves, and antimicrobial treatment of cows were associated with increased prevalence of resistant E. coli and Enterococcus spp. Despite the low overall usage of antimicrobials in Latvia, the high rates of antimicrobial resistance in fecal indicators and Campylobacter, in addition to the high prevalence of ESBL-producing E. coli, highlights the necessity for the prudent use of antimicrobials in dairy farms in Latvia.
Margarita Terentjeva; Madara Streikiša; Jeļena Avsejenko; Jūlija Trofimova; Kaspars Kovalenko; Didzis Elferts; Aivars Bērziņš. Prevalence and Antimicrobial Resistance of Escherichia coli, Enterococcus spp. and the Major Foodborne Pathogens in Calves in Latvia. Foodborne Pathogens and Disease 2019, 16, 35 -41.
AMA StyleMargarita Terentjeva, Madara Streikiša, Jeļena Avsejenko, Jūlija Trofimova, Kaspars Kovalenko, Didzis Elferts, Aivars Bērziņš. Prevalence and Antimicrobial Resistance of Escherichia coli, Enterococcus spp. and the Major Foodborne Pathogens in Calves in Latvia. Foodborne Pathogens and Disease. 2019; 16 (1):35-41.
Chicago/Turabian StyleMargarita Terentjeva; Madara Streikiša; Jeļena Avsejenko; Jūlija Trofimova; Kaspars Kovalenko; Didzis Elferts; Aivars Bērziņš. 2019. "Prevalence and Antimicrobial Resistance of Escherichia coli, Enterococcus spp. and the Major Foodborne Pathogens in Calves in Latvia." Foodborne Pathogens and Disease 16, no. 1: 35-41.
Acanthamoeba are free-living amoebae found in various ecological environments, and they are major microbial predators. Some Acanthamoeba species can cause keratitis, granulomatous amoebic encephalitis, and infections of the organs. This is the first study in Latvia describing Acanthamoeba detection by molecular techniques and genotyping using tap water from different sources. An 18S rRNA gene investigation of the 20 Acanthamoeba isolates suggested that 19 belonged to the hazardous T4 genotype and one sample was T12. The results indicate that tap water in Latvia may be a source of Acanthamoeba that is potentially pathogenic to humans. All obtained sequences were submitted to the GenBank database under the following accession numbers: MG386295–MG386314.
Inese Gavarāne; Jūlija Trofimova; Artjoms Mališevs; Olga Valciņa; Muza Kirjušina; Ilze Rubeniņa; Aivars Bērziņš. DNA extraction from amoebal isolates and genotype determination of Acanthamoeba from tap water in Latvia. Parasitology Research 2018, 117, 3299 -3303.
AMA StyleInese Gavarāne, Jūlija Trofimova, Artjoms Mališevs, Olga Valciņa, Muza Kirjušina, Ilze Rubeniņa, Aivars Bērziņš. DNA extraction from amoebal isolates and genotype determination of Acanthamoeba from tap water in Latvia. Parasitology Research. 2018; 117 (10):3299-3303.
Chicago/Turabian StyleInese Gavarāne; Jūlija Trofimova; Artjoms Mališevs; Olga Valciņa; Muza Kirjušina; Ilze Rubeniņa; Aivars Bērziņš. 2018. "DNA extraction from amoebal isolates and genotype determination of Acanthamoeba from tap water in Latvia." Parasitology Research 117, no. 10: 3299-3303.
An ultra-sensitive mass spectrometric confirmation and quantification method for the determination of selected fluoroquinolones-enrofloxacin and its main metabolite ciprofloxacin-was developed and validated in poultry meat samples. The achieved limits of quantification were 1 ng kg-1 for enrofloxacin and 10 ng kg-1 for ciprofloxacin. The analysis of 40 retail poultry samples originating from Estonia, Latvia, Lithuania, Poland and France revealed that 93% of samples contained residues of enrofloxacin in the range from 3.3 to 1126 ng kg-1. Previous studies have shown high levels of antimicrobial resistance to fluoroquinolones, particularly in Campylobacter spp. and various faecal indicators isolated from broiler meat. Consequently, the revealed widespread usage of fluoroquinolones in the poultry industry may result in the further emergence of antimicrobial resistance of Campylobacter in the food chain.
Iveta Pugajeva; Jeļena Avsejenko; Elza Judjallo; Aivars Bērziņš; Elena Bartkiene; Vadims Bartkevics. High occurrence rates of enrofloxacin and ciprofloxacin residues in retail poultry meat revealed by an ultra-sensitive mass-spectrometric method, and antimicrobial resistance to fluoroquinolones in Campylobacter spp. Food Additives & Contaminants: Part A 2018, 35, 1107 -1115.
AMA StyleIveta Pugajeva, Jeļena Avsejenko, Elza Judjallo, Aivars Bērziņš, Elena Bartkiene, Vadims Bartkevics. High occurrence rates of enrofloxacin and ciprofloxacin residues in retail poultry meat revealed by an ultra-sensitive mass-spectrometric method, and antimicrobial resistance to fluoroquinolones in Campylobacter spp. Food Additives & Contaminants: Part A. 2018; 35 (6):1107-1115.
Chicago/Turabian StyleIveta Pugajeva; Jeļena Avsejenko; Elza Judjallo; Aivars Bērziņš; Elena Bartkiene; Vadims Bartkevics. 2018. "High occurrence rates of enrofloxacin and ciprofloxacin residues in retail poultry meat revealed by an ultra-sensitive mass-spectrometric method, and antimicrobial resistance to fluoroquinolones in Campylobacter spp." Food Additives & Contaminants: Part A 35, no. 6: 1107-1115.
Baiba Fogele; Rita Granta; Olga Valciņa; Aivars Bērziņš. Occurrence and diversity of Bacillus cereus and moulds in spices and herbs. Food Control 2018, 83, 69 -74.
AMA StyleBaiba Fogele, Rita Granta, Olga Valciņa, Aivars Bērziņš. Occurrence and diversity of Bacillus cereus and moulds in spices and herbs. Food Control. 2018; 83 ():69-74.
Chicago/Turabian StyleBaiba Fogele; Rita Granta; Olga Valciņa; Aivars Bērziņš. 2018. "Occurrence and diversity of Bacillus cereus and moulds in spices and herbs." Food Control 83, no. : 69-74.
An increasing number of bacteria have been described as benefiting from interaction with free-living amoeba. The most common association between free-living amoeba and microorganisms is interaction of various non-pathogenic and pathogenic bacterial species with amoeba. Various pathogenic bacterial species have capacity to resist digestion by free-living amoeba, which has been observed by many researchers. Also, several of these pathogens are able to resist digestion by macrophages. In addition, free-living amoeba have been associated with several diseases in humans. Acanthamniioeba castella is an important predator of bacteria. It is a ubiquitous organism in water, soil, and air. Attention from a public health perspective is needed by investigation of interaction of foodborne pathogens and free-living amoeba. Bacteria can use free-living amoeba as reservoirs, mediators or vehicles, an infection route, “biological gym” and evolutionary crib or interaction may result in a close endosymbiotic relationship. The purpose of this review is to describe the interaction mechanisms between free-living amoeba and common bacteria species that survive in host cells.
Ilze Rubeniņa; Muza Kirjušina; Aivars Bērziņš; Olga Valciņa; Inese Jahundoviča. Relationships between Free-Living Amoeba and their Intracellular Bacteria. Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. 2017, 71, 259 -265.
AMA StyleIlze Rubeniņa, Muza Kirjušina, Aivars Bērziņš, Olga Valciņa, Inese Jahundoviča. Relationships between Free-Living Amoeba and their Intracellular Bacteria. Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.. 2017; 71 (4):259-265.
Chicago/Turabian StyleIlze Rubeniņa; Muza Kirjušina; Aivars Bērziņš; Olga Valciņa; Inese Jahundoviča. 2017. "Relationships between Free-Living Amoeba and their Intracellular Bacteria." Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. 71, no. 4: 259-265.
Introduction and objective Salmonella is a foodborne pathogen which causes gastrointestinal illness in consumers, and exhibits resistance to antimicrobials of eterinary and clinical significance. The aim of this study is to detect the prevalence and antimicrobial resistance of Salmonella isolates from meat in Latvia. Material and Methods A total of 3,152 samples of raw and ready-to-eat (RTE) meats were collected during the official control and in-house control procedures in 2015. Samples were tested in accordance with ISO 6579:2002. All S. Typhimurium, S. Enteritidis and other isolates recovered from the official control samples (S. Derby, S. Give) were tested for antimicrobial resistance. The minimum inhibitory concentration (MIC) values were investigated in line with the requirements of the European Committee on Antimicrobial Susceptibility Testing (EUCAST). Results The prevalence of Salmonella was 0.8% (25/3152). The highest prevalence (1.5%) of Salmonella was found in minced meat and meat preparations (7/481), while the lowest (0%) in frozen meat and meat preparations (0/349) and RTE meats (0/364). The most common serovars were S. Typhimurium (36%, 9/25) and S. Derby (32%, 8/25). In total, 62% (13/21) of Salmonella isolates were resistant to at least one antimicrobial agent. Altogether, 40% (8/20) of isolates were resistant to sulfamethoxazole, 25% (5/20) to nalidixic acid, ciprofloxacin, ampicillin and 20% (4/20) to tetracycline. All isolates were susceptible to ceftazidime, cefotaxime, meropenem, azithromycin and tigecycline. S. Typhimurium exhibited antimicrobial resistance more often (87.5%) than other serovars. Conclusions The study shows that the presence of Salmonella in meat, together with the high prevalence of resistant strains, is a significant public health related issue in Latvia
Margarita Terentjeva; Jeļena Avsejenko; Madara Streikiša; Andra Utināne; Kaspars Kovalenko; Aivars Bērziņš. Prevalence and antimicrobial resistance of Salmonella in meat and meat products in Latvia. Annals of Agricultural and Environmental Medicine 2017, 24, 317 -321.
AMA StyleMargarita Terentjeva, Jeļena Avsejenko, Madara Streikiša, Andra Utināne, Kaspars Kovalenko, Aivars Bērziņš. Prevalence and antimicrobial resistance of Salmonella in meat and meat products in Latvia. Annals of Agricultural and Environmental Medicine. 2017; 24 (2):317-321.
Chicago/Turabian StyleMargarita Terentjeva; Jeļena Avsejenko; Madara Streikiša; Andra Utināne; Kaspars Kovalenko; Aivars Bērziņš. 2017. "Prevalence and antimicrobial resistance of Salmonella in meat and meat products in Latvia." Annals of Agricultural and Environmental Medicine 24, no. 2: 317-321.
Legionella pneumophila is an environmental pathogen of engineered water systems that can cause different forms of legionellosis - from mild fever to potentially lethal pneumonia. Low concentrations of legionellae in natural habitats can increase markedly in engineered hot water systems where water temperatures are below 55 °C. In the current study, we aimed to investigate the influence of sampling season, hot water temperature and sampling protocol on occurrence of L. pneumophila. A total of 120 hot water samples from 20 apartment buildings were collected in two sampling periods - winter 2014 (n = 60) and summer 2015 (n = 60). Significantly higher occurrence of L. pneumophila was observed in summer 2015. Significant differences in temperature for negative and positive samples were not observed, which can be explained by low water temperatures at the point of water consumption. Temperature above 55 °C was observed only once, for all other sampling events it ranged from 14 °C to 53 °C.
Daina Pūle; Olga Valciņa; Aivars Bērziņš; Ludmila Vīksna; Angelika Krūmiņa. Influence of Sampling Season and Sampling Protocol on Detection of Legionella Pneumophila Contamination in Hot Water / Paraugu Ņemšanas Sezonalitātes Un Paraugu Ņemšanas Metodes Ietekme Uz Legionella Pneumophila Kontaminācijas Noteikšanu Karstajš Ūdenī. Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. 2016, 70, 227 -231.
AMA StyleDaina Pūle, Olga Valciņa, Aivars Bērziņš, Ludmila Vīksna, Angelika Krūmiņa. Influence of Sampling Season and Sampling Protocol on Detection of Legionella Pneumophila Contamination in Hot Water / Paraugu Ņemšanas Sezonalitātes Un Paraugu Ņemšanas Metodes Ietekme Uz Legionella Pneumophila Kontaminācijas Noteikšanu Karstajš Ūdenī. Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.. 2016; 70 (4):227-231.
Chicago/Turabian StyleDaina Pūle; Olga Valciņa; Aivars Bērziņš; Ludmila Vīksna; Angelika Krūmiņa. 2016. "Influence of Sampling Season and Sampling Protocol on Detection of Legionella Pneumophila Contamination in Hot Water / Paraugu Ņemšanas Sezonalitātes Un Paraugu Ņemšanas Metodes Ietekme Uz Legionella Pneumophila Kontaminācijas Noteikšanu Karstajš Ūdenī." Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. 70, no. 4: 227-231.
Continuous environmental exposure of humans to Legionella may induce immune responses and generation of antibodies. The aim of this study was to investigate the seroprevalence of Legionella pneumophila serogroups (SG) 1–6 in the general healthy population and identify the associated host-related and environmental risk factors. L. pneumophila SG 1–6 seroprevalence among a total of 2007 blood samples collected from healthy donors was 4.8%. Seroprevalence was higher in women (5.9%) than men (3.3%) and in areas with a larger number of inhabitants, ranging from 3.5% in rural regions to 6.8% in the capital, Riga. Blood samples from inhabitants of apartment buildings tested positive for L. pneumophila in more cases (5.8%) compared to those from inhabitants of single-family homes (2.7%). Residents of buildings with a municipal hot water supply system were more likely to be seropositive for L. pneumophila (OR = 3.16, 95% CI 1.26–7.91). Previous episodes of fever were additionally identified as a risk factor (OR = 2.42, 95% CI 1.43–4.1). In conclusion, centralized hot water supply, female gender and previous episodes of fever were determined as the main factors associated with L. pneumophila seropositivity in our study population.
Olga Valciņa; Daina Pūle; Irina Lucenko; Dita Krastiņa; Žanete Šteingolde; Angelika Krumina; Aivars Berzins. Legionella pneumophila Seropositivity-Associated Factors in Latvian Blood Donors. International Journal of Environmental Research and Public Health 2015, 13, 58 .
AMA StyleOlga Valciņa, Daina Pūle, Irina Lucenko, Dita Krastiņa, Žanete Šteingolde, Angelika Krumina, Aivars Berzins. Legionella pneumophila Seropositivity-Associated Factors in Latvian Blood Donors. International Journal of Environmental Research and Public Health. 2015; 13 (1):58.
Chicago/Turabian StyleOlga Valciņa; Daina Pūle; Irina Lucenko; Dita Krastiņa; Žanete Šteingolde; Angelika Krumina; Aivars Berzins. 2015. "Legionella pneumophila Seropositivity-Associated Factors in Latvian Blood Donors." International Journal of Environmental Research and Public Health 13, no. 1: 58.
The aim of this study was to detect the prevalence of Salmonella spp., Listeria monocytogenes, and Yersinia enterocolitica in freshwater fish in Latvia. In total, 235 samples, including freshly caught fish from fives lakes (n = 129) and fish from retail markets (n = 106), were collected from April 2014 to December 2014 in Latvia. Samples were tested according to International Organization for Standardization methods. No Salmonella spp. were found in fresh fish from lakes or in commercially available fish. In contrast, the overall prevalence of L. monocytogenes and Y. enterocolitica in freshwater fish was 13% (30 of 235) and 14% (34 of 235), respectively, and no significant difference between the prevalence of L. monocytogenes and Y. enterocolitica was observed (P > 0.05). All Y. enterocolitica isolates belonged to the nonpathogenic 1A biotype. Molecular serotyping of L. monocytogenes revealed that the most distributed serogroup was 1/2a-3a (65%), followed by 1/2c-3c (25%), 1/2b-3b (5%), and 4b, 4d, 4e (5%). The prevalence of L. monocytogenes and Y. enterocolitica in freshwater lake fish was 2% (2 of 129) and 3% (4 of 129), respectively. In contrast, the prevalence of L. monocytogenes and Y. enterocolitica in fish at retail markets was 26% (28 of 106) and 28% (30 of 106), respectively. In retail samples, 9 of 58 positive fish contained both L. monocytogenes and Y. enterocolitica. In general, differences in the prevalences of L. monocytogenes and Y. enterocolitica in retail samples were significantly higher than those in freshly caught fish (P < 0.05). The results of this study indicate that freshwater fish could be an important source of Y. enterocolitica and L. monocytogenes for consumers in Latvia.
Margarita Terentjeva; Inga Eizenberga; Olga Valciņa; Aleksandr Novoslavskij; Vita Strazdiņa; Aivars Bērziņš. Prevalence of Foodborne Pathogens in Freshwater Fish in Latvia. Journal of Food Protection 2015, 78, 2093 -2098.
AMA StyleMargarita Terentjeva, Inga Eizenberga, Olga Valciņa, Aleksandr Novoslavskij, Vita Strazdiņa, Aivars Bērziņš. Prevalence of Foodborne Pathogens in Freshwater Fish in Latvia. Journal of Food Protection. 2015; 78 (11):2093-2098.
Chicago/Turabian StyleMargarita Terentjeva; Inga Eizenberga; Olga Valciņa; Aleksandr Novoslavskij; Vita Strazdiņa; Aivars Bērziņš. 2015. "Prevalence of Foodborne Pathogens in Freshwater Fish in Latvia." Journal of Food Protection 78, no. 11: 2093-2098.
Fish plays an important role in the human diet, and there is an observed increase in the consumption of fish per capita in Europe. However, intensive growth of industry and agriculture may cause contamination of natural and human-made aquatic environments, and may affect not only the health of fish, but also raise safety concerns with regard to fish used for human consumption. It is well known that fish and fish products are often associated with human diseases. Thus, it is necessary to study the prevalence of pathogens in fish to ensure the safety of fish products and environments. Microbial assessment of fish also gives additional information about the hygienic status of environments, including lakes, rivers, ponds, and fish farms. Detection of pathogenic microorganisms or changes in natural microflora in the water environment could be an important indicator of possible contamination. The aim of this review was to describe and discuss the five most relevant bacterial genera and species linked to aquatic environments—Vibrio spp., Listeria monocytogenes, Yersinia spp., pathogenic Salmonella serovars, and Clostridium botulinum—causing human foodborne diseases.
Aleksandr Novoslavskij; Margarita Terentjeva; Inga Eizenberga; Olga Valciņa; Vadims Bartkevics; Aivars Bērziņš. Major foodborne pathogens in fish and fish products: a review. Annals of Microbiology 2015, 66, 1 -15.
AMA StyleAleksandr Novoslavskij, Margarita Terentjeva, Inga Eizenberga, Olga Valciņa, Vadims Bartkevics, Aivars Bērziņš. Major foodborne pathogens in fish and fish products: a review. Annals of Microbiology. 2015; 66 (1):1-15.
Chicago/Turabian StyleAleksandr Novoslavskij; Margarita Terentjeva; Inga Eizenberga; Olga Valciņa; Vadims Bartkevics; Aivars Bērziņš. 2015. "Major foodborne pathogens in fish and fish products: a review." Annals of Microbiology 66, no. 1: 1-15.
Kaspars Kovalenko; Mati Roasto; Stella Šantare; Aivars Bērziņš; Ari Hörman. Campylobacter species and their antimicrobial resistance in Latvian broiler chicken production. Food Control 2014, 46, 86 -90.
AMA StyleKaspars Kovalenko, Mati Roasto, Stella Šantare, Aivars Bērziņš, Ari Hörman. Campylobacter species and their antimicrobial resistance in Latvian broiler chicken production. Food Control. 2014; 46 ():86-90.
Chicago/Turabian StyleKaspars Kovalenko; Mati Roasto; Stella Šantare; Aivars Bērziņš; Ari Hörman. 2014. "Campylobacter species and their antimicrobial resistance in Latvian broiler chicken production." Food Control 46, no. : 86-90.
Aivars Bērziņš; Janne Lundén; Hannu Korkeala. Official Control: B. Organization of Official Control. Meat Inspection and Control in the Slaughterhouse 2014, 556 -561.
AMA StyleAivars Bērziņš, Janne Lundén, Hannu Korkeala. Official Control: B. Organization of Official Control. Meat Inspection and Control in the Slaughterhouse. 2014; ():556-561.
Chicago/Turabian StyleAivars Bērziņš; Janne Lundén; Hannu Korkeala. 2014. "Official Control: B. Organization of Official Control." Meat Inspection and Control in the Slaughterhouse , no. : 556-561.
Contamination patterns of Listeria monocytogenes were studied in a cold-smoked pork processing plant to identify the sources and possible reasons for the contamination. Environmental sampling combined with pulsed-field gel electrophoresis (PFGE) subtyping and serotyping were applied to investigate the genetic diversity of L. monocytogenes in the plant environment and ready-to-eat (RTE) cold-smoked pork products. A total of 183 samples were collected for contamination analyses, including samples of the product at different stages during manufacture (n = 136) and environmental samples (n = 47) in 2009. L. monocytogenes isolates, previously recovered from 73 RTE cold-smoked pork samples and collected from the same meat processing plant in 2004, were included in this study. The brining machine and personnel working with brining procedures were the most contaminated places with L. monocytogenes. The overall prevalence of L. monocytogenes in raw pork (18%) increased to 60% after the brining injections. The brining machine harbored six different PFGE types belonging to serotypes 1/2a, 1/2c, 4b, and 4d, which were found on the feeding teeth, smooth surfaces, and spaces of the machine, thus potentially facilitating dissemination of L. monocytogenes contamination. Two PFGE types (2 and 8) belonging to serotypes 1/2a and 1/2c were recovered from RTE cold-smoked pork collected in 2004, and from surfaces of the brining machine sampled in 2009, and may indicate the presence of persistent L. monocytogenes strains in the plant. Due to poor hygiene design, removal of the brining machine from the production of cold-smoked meat products should be considered to reduce L. monocytogenes contamination in the finished products.
Aivars Bērziņš; Sanna Hellström; Indulis Siliņš; Hannu Korkeala. Contamination Patterns of Listeria monocytogenes in Cold-Smoked Pork Processing. Journal of Food Protection 2010, 73, 2103 -2109.
AMA StyleAivars Bērziņš, Sanna Hellström, Indulis Siliņš, Hannu Korkeala. Contamination Patterns of Listeria monocytogenes in Cold-Smoked Pork Processing. Journal of Food Protection. 2010; 73 (11):2103-2109.
Chicago/Turabian StyleAivars Bērziņš; Sanna Hellström; Indulis Siliņš; Hannu Korkeala. 2010. "Contamination Patterns of Listeria monocytogenes in Cold-Smoked Pork Processing." Journal of Food Protection 73, no. 11: 2103-2109.
The prevalence of Yersinia enterocolitica and Yersinia pseudotuberculosis was determined in slaughter pigs from 47 farms in Latvia. Tonsils from 404 pigs representing farms from four geographical areas (Kurzeme, Latgale, Vidzeme, and Zemgale) were collected at slaughter in 2007 and 2008. The prevalences of Y. enterocolitica and Y. pseudotuberculosis were 35 and 3%, respectively. All Y. enterocolitica isolates belonged to bioserotype 4/O:3. Y. enterocolitica was recovered from 35 of 47 pig farms, and Y. pseudotuberculosis was found on 6 farms. The prevalence of Yersinia was highest in Latgale (90%) followed by Kurzeme (39%), Zemgale (33%), and Vidzeme (21%). Y. enterocolitica 4/O:3 was sensitive to amoxicillin–clavulanic acid, aztreonam, cefotaxime, ceftriaxone, chloramphenicol, ciprofloxacin, nalidixic acid, trimethoprim, and trimethoprimsulfamethoxazole and resistant to ampicillin, cephalothin, erythromycin, streptomycin, sulfamethoxazole, and tetracycline. Y. pseudotuberculosis exhibited resistance to erythromycin and sulfamethoxazole but not to the other antimicrobial agents tested. The results of this study are a valuable starting point for monitoring the prevalence and antimicrobial resistance of Yersinia in pigs in Latvia.
Margarita Terentjeva; Aivars Bērziņš. Prevalence and Antimicrobial Resistance of Yersinia enterocolitica and Yersinia pseudotuberculosis in Slaughter Pigs in Latvia. Journal of Food Protection 2010, 73, 1335 -1338.
AMA StyleMargarita Terentjeva, Aivars Bērziņš. Prevalence and Antimicrobial Resistance of Yersinia enterocolitica and Yersinia pseudotuberculosis in Slaughter Pigs in Latvia. Journal of Food Protection. 2010; 73 (7):1335-1338.
Chicago/Turabian StyleMargarita Terentjeva; Aivars Bērziņš. 2010. "Prevalence and Antimicrobial Resistance of Yersinia enterocolitica and Yersinia pseudotuberculosis in Slaughter Pigs in Latvia." Journal of Food Protection 73, no. 7: 1335-1338.