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Listeria spp. is a diverse genus of Gram-positive bacteria commonly present in the environment while L. monocytogenes and L. ivanovii are well known human and ruminant pathogens. The aim of the present study was to reveal the prevalence and genetic diversity of L. monocytogenes and other Listeria spp. and to identify the factors related to the abundance of pathogen at cattle farms. A total of 521 animal and environmental samples from 27 meat and dairy cattle farms were investigated and the genetic diversity of L. monocytogenes isolates was studied with WGS. The prevalence of Listeria was 58.9%, while of L. monocytogenes it was −11%. The highest prevalence of L. monocytogenes was found in the environment—soil samples near to manure storage (93%), mixed feed from the feeding trough and hay (29%), water samples from farms drinking trough (28%) and cattle feces (28%). Clonal complexes (CC) of CC37 (30%), CC11 (20%) and CC18 (17%) (all IIa serogroup) were predominant L. monocytogenes clones. CC18, CC37 and CC8 were isolated from case farms and CC37, CC11 and CC18 from farms without listeriosis history. Only one hypervirulent CC4 (1%) was isolated from the case farm. Sequence types (STs) were not associated with the isolation source, except for ST7, which was significantly associated with soil (p< 0.05). The contamination of soil, feeding tables and troughs with L. monocytogenes was associated with an increased prevalence of L. monocytogenes at farms. Our study indicates the importance of hygienic practice in the prevention of the dissemination of L. monocytogenes in the cattle farm environment.
Margarita Terentjeva; Žanete Šteingolde; Irēna Meistere; Didzis Elferts; Jeļena Avsejenko; Madara Streikiša; Silva Gradovska; Laura Alksne; Juris Ķibilds; Aivars Bērziņš. Prevalence, Genetic Diversity and Factors Associated with Distribution of Listeria monocytogenes and Other Listeria spp. in Cattle Farms in Latvia. Pathogens 2021, 10, 851 .
AMA StyleMargarita Terentjeva, Žanete Šteingolde, Irēna Meistere, Didzis Elferts, Jeļena Avsejenko, Madara Streikiša, Silva Gradovska, Laura Alksne, Juris Ķibilds, Aivars Bērziņš. Prevalence, Genetic Diversity and Factors Associated with Distribution of Listeria monocytogenes and Other Listeria spp. in Cattle Farms in Latvia. Pathogens. 2021; 10 (7):851.
Chicago/Turabian StyleMargarita Terentjeva; Žanete Šteingolde; Irēna Meistere; Didzis Elferts; Jeļena Avsejenko; Madara Streikiša; Silva Gradovska; Laura Alksne; Juris Ķibilds; Aivars Bērziņš. 2021. "Prevalence, Genetic Diversity and Factors Associated with Distribution of Listeria monocytogenes and Other Listeria spp. in Cattle Farms in Latvia." Pathogens 10, no. 7: 851.
Brucellosis due to Brucella suis biovar 2 is one of the most important endemic diseases in wild boar (Sus scrofa) populations in Europe. The aim of the present study was to determine the seroprevalence of brucellosis in wild boars in the eastern part of Latvia. Wild boars killed by hunters in the period from January to April 2015 (n = 877) and from March to April in 2016 (n = 167) were examined for antibodies against B. suis by the Rose Bengal test (RBT), a complement fixation test (CFT), and by enzyme-linked immunosorbent assays. In 2015, 199 samples (22.7%) were positive by RBT and/or CFT while 36 samples (21.6%) were seropositive in 2016. Of the Brucella seropositive samples from 2015 and 2016 (n = 235), 162 (68.9%) were also seropositive to Yersinia enterocolitica. Considering cross-reactivity of serological tests, the seroprevalence of B. suis biovar 2 exposure in wild boars in the eastern part of Latvia was calculated to 14.0% in 2015 and 9.6% in 2016. From selected seropositive samples (42 in 2015 and 36 in 2016) total DNA was extracted and analyzed with an IS711-based nested polymerase chain reaction (PCR) assay. Species and biovar identification was conducted for bacteria isolated in monoculture from PCR positive samples by species specific primers and Bruce-ladder multiplex PCR. Brucella suis biovar 2 was isolated from 12/20 samples in 2015 and 9/9 samples in 2016. The average seroprevalence was relatively low compared to that found in certain other European countries. Males and females had an equal level of seropositivity, but a positive age-trend was observed for both males and females.
Lelde Grantina-Ievina; Jelena Avsejenko; Svetlana Cvetkova; Dita Krastina; Madara Streikisa; Zanete Steingolde; Indra Vevere; Ieva Rodze. Seroprevalence of Brucella suis in eastern Latvian wild boars (Sus scrofa). Acta Veterinaria Scandinavica 2018, 60, 1 -6.
AMA StyleLelde Grantina-Ievina, Jelena Avsejenko, Svetlana Cvetkova, Dita Krastina, Madara Streikisa, Zanete Steingolde, Indra Vevere, Ieva Rodze. Seroprevalence of Brucella suis in eastern Latvian wild boars (Sus scrofa). Acta Veterinaria Scandinavica. 2018; 60 (1):1-6.
Chicago/Turabian StyleLelde Grantina-Ievina; Jelena Avsejenko; Svetlana Cvetkova; Dita Krastina; Madara Streikisa; Zanete Steingolde; Indra Vevere; Ieva Rodze. 2018. "Seroprevalence of Brucella suis in eastern Latvian wild boars (Sus scrofa)." Acta Veterinaria Scandinavica 60, no. 1: 1-6.
Continuous environmental exposure of humans to Legionella may induce immune responses and generation of antibodies. The aim of this study was to investigate the seroprevalence of Legionella pneumophila serogroups (SG) 1–6 in the general healthy population and identify the associated host-related and environmental risk factors. L. pneumophila SG 1–6 seroprevalence among a total of 2007 blood samples collected from healthy donors was 4.8%. Seroprevalence was higher in women (5.9%) than men (3.3%) and in areas with a larger number of inhabitants, ranging from 3.5% in rural regions to 6.8% in the capital, Riga. Blood samples from inhabitants of apartment buildings tested positive for L. pneumophila in more cases (5.8%) compared to those from inhabitants of single-family homes (2.7%). Residents of buildings with a municipal hot water supply system were more likely to be seropositive for L. pneumophila (OR = 3.16, 95% CI 1.26–7.91). Previous episodes of fever were additionally identified as a risk factor (OR = 2.42, 95% CI 1.43–4.1). In conclusion, centralized hot water supply, female gender and previous episodes of fever were determined as the main factors associated with L. pneumophila seropositivity in our study population.
Olga Valciņa; Daina Pūle; Irina Lucenko; Dita Krastiņa; Žanete Šteingolde; Angelika Krumina; Aivars Berzins. Legionella pneumophila Seropositivity-Associated Factors in Latvian Blood Donors. International Journal of Environmental Research and Public Health 2015, 13, 58 .
AMA StyleOlga Valciņa, Daina Pūle, Irina Lucenko, Dita Krastiņa, Žanete Šteingolde, Angelika Krumina, Aivars Berzins. Legionella pneumophila Seropositivity-Associated Factors in Latvian Blood Donors. International Journal of Environmental Research and Public Health. 2015; 13 (1):58.
Chicago/Turabian StyleOlga Valciņa; Daina Pūle; Irina Lucenko; Dita Krastiņa; Žanete Šteingolde; Angelika Krumina; Aivars Berzins. 2015. "Legionella pneumophila Seropositivity-Associated Factors in Latvian Blood Donors." International Journal of Environmental Research and Public Health 13, no. 1: 58.