Leaf area index (LAI) is one of the most important plant parameters when observing agricultural crops and a decisive factor for yield estimates. Remote-sensing data provide spectral information on large areas and allow for a detailed quantitative assessment of LAI and other plant parameters. The present study compared support vector regression (SVR), random forest regression (RFR), and partial least-squares regression (PLSR) and their achieved model qualities for the assessment of LAI from wheat reflectance spectra. In this context, the validation technique used for verifying the accuracy of an empirical–statistical regression model was very important in order to allow the spatial transferability of models to unknown data. Thus, two different validation methods, leave-one-out cross-validation (cv) and independent validation (iv), were performed to determine model accuracy. The LAI and field reflectance spectra of 124 plots were collected from four fields during two stages of plant development in 2011 and 2012. In the case of cross-validation for the separate years, as well as the entire data set, SVR provided the best results (2011: R²_cv = 0.739, 2012: R²_cv = 0.85, 2011 and 2012: R²_cv = 0.944). Independent validation of the data set from both years led to completely different results. The accuracy of PLSR (R²_iv = 0.912) and RFR (R²_iv = 0.770) remained almost at the same level as that of cross-validation, while SVR showed a clear decline in model performance (R²_iv = 0.769). The results indicate that regression model robustness largely depends on the applied validation approach and the data range of the LAI used for model building.

The HyPlant imaging spectrometer is a high-performance airborne instrument consisting of two sensor modules. The DUAL module records hyperspectral data in the spectral range from 400–2500 nm, which is useful to derive biochemical and structural plant properties. In parallel, the FLUO module acquires data in the red and near infrared range (670–780 nm), with a distinctly higher spectral sampling interval and finer spectral resolution. The technical specifications of HyPlant FLUO allow for the retrieval of sun-induced chlorophyll fluorescence (SIF), a small signal emitted by plants, which is directly linked to their photosynthetic efficiency. The combined use of both HyPlant modules opens up new opportunities in plant science. The processing of HyPlant image data, however, is a rather complex procedure, and, especially for the FLUO module, a precise characterization and calibration of the sensor is of utmost importance. The presented study gives an overview of this unique high-performance imaging spectrometer, introduces an automatized processing chain, and gives an overview of the different processing steps that must be executed to generate the final products, namely top of canopy (TOC) radiance, TOC reflectance, reflectance indices and SIF maps.

Unmanned aerial vehicles (UAVs) open new opportunities in precision agriculture and phenotyping because of their flexibility and low cost. In this study, the potential of UAV imagery was evaluated to quantify lodging percentage and lodging severity of barley using structure from motion (SfM) techniques. Traditionally, lodging quantification is based on time-consuming manual field observations. Our UAV-based approach makes use of a quantitative threshold to determine lodging percentage in a first step. The derived lodging estimates showed a very high correlation to reference data (R² = 0.96, root mean square error (RMSE) = 7.66%) when applied to breeding trials, which could also be confirmed under realistic farming conditions. As a second step, an approach was developed that allows the assessment of lodging severity, information that is important to estimate yield impairment, which also takes the intensity of lodging events into account. Both parameters were tested on three ground sample distances. The lowest spatial resolution acquired from the highest flight altitude (100 m) still led to high accuracy, which increases the practicability of the method for large areas. Our new lodging assessment procedure can be used for insurance applications, precision farming, and selecting for genetic lines with greater lodging resistance in breeding research.

Land surface temperature (LST) is a fundamental parameter within the system of the Earth’s surface and atmosphere, which can be used to describe the inherent physical processes of energy and water exchange. The need for LST has been increasingly recognised in agriculture, as it affects the growth phases of crops and crop yields. However, challenges in overcoming the large discrepancies between the retrieved LST and ground truth data still exist. Precise LST measurement depends mainly on accurately deriving the surface emissivity, which is very dynamic due to changing states of land cover and plant development. In this study, we present an LST retrieval algorithm for the combined use of multispectral optical and thermal UAV images, which has been optimised for operational applications in agriculture to map the heterogeneous and diverse agricultural crop systems of a research campus in Germany (April 2018). We constrain the emissivity using certain NDVI thresholds to distinguish different land surface types. The algorithm includes atmospheric corrections and environmental thermal emissions to minimise the uncertainties. In the analysis, we emphasise that the omission of crucial meteorological parameters and inaccurately determined emissivities can lead to a considerably underestimated LST; however, if the emissivity is underestimated, the LST can be overestimated. The retrieved LST is validated by reference temperatures from nearby ponds and weather stations. The validation of the thermal measurements indicates a mean absolute error of about 0.5 K. The novelty of the dual sensor system is that it simultaneously captures highly spatially resolved optical and thermal images, in order to construct the precise LST ortho-mosaics required to monitor plant diseases and drought stress and validate airborne and satellite data.

Mapping crop variables at different growth stages is crucial to inform farmers and plant breeders about the crop status. For mapping purposes, inversion of canopy radiative transfer models (RTMs) is a viable alternative to parametric and non-parametric regression models, which often lack transferability in time and space. Due to the physical nature of RTMs, inversion outputs can be delivered in sound physical units that reflect the underlying processes in the canopy. In this study, we explored the capabilities of the coupled leaf–canopy RTM PROSAIL applied to high-spatial-resolution (0.015 m) multispectral unmanned aerial vehicle (UAV) data to retrieve the leaf chlorophyll content (LCC), leaf area index (LAI) and canopy chlorophyll content (CCC) of sweet and silage maize throughout one growing season. Two different retrieval methods were tested: (i) applying the RTM inversion scheme to mean reflectance data derived from single breeding plots (mean reflectance approach) and (ii) applying the same inversion scheme to an orthomosaic to separately retrieve the target variables for each pixel of the breeding plots (pixel-based approach). For LCC retrieval, soil and shaded pixels were removed by applying simple vegetation index thresholding. Retrieval of LCC from UAV data yielded promising results compared to ground measurements (sweet maize RMSE = 4.92 µg/m², silage maize RMSE = 3.74 µg/m²) when using the mean reflectance approach. LAI retrieval was more challenging due to the blending of sunlit and shaded pixels present in the UAV data, but worked well at the early developmental stages (sweet maize RMSE = 0.70 $\mathrm{m}$${}^2$/$\mathrm{m}$${}^2$, silage RMSE = 0.61 $\mathrm{m}$${}^2$/$\mathrm{m}$${}^2$ across all dates). CCC retrieval significantly benefited from the pixel-based approach compared to the mean reflectance approach (RMSEs decreased from 45.6 to 33.1 µg/m²). We argue that high-resolution UAV imagery is well suited for LCC retrieval, as shadows and background soil can be precisely removed, leaving only green plant pixels for the analysis. As for retrieving LAI, it proved to be challenging for two distinct varieties of maize that were characterized by contrasting canopy geometry.

Introduction: Detecting and monitoring crop stress is crucial for ensuring sufficient and sustainable crop production. Recent advancements in unoccupied aerial vehicle (UAV) technology provide a promising approach to map key crop traits indicative of stress. While using single optical sensors mounted on UAVs could be sufficient to monitor crop status in a general sense, implementing multiple sensors that cover various spectral optical domains allow for a more precise characterization of the interactions between crops and biotic or abiotic stressors. Given the novelty of synergistic sensor technology for crop stress detection, standardized procedures outlining their optimal use are currently lacking. Materials and methods: This study explores the key aspects of acquiring high-quality multi-sensor data, including the importance of mission planning, sensor characteristics, and ancillary data. It also details essential data pre-processing steps like atmospheric correction and highlights best practices for data fusion and quality control. Results: Successful multi-sensor data acquisition depends on optimal timing, appropriate sensor calibration, and the use of ancillary data such as ground control points and weather station information. When fusing different sensor data it should be conducted at the level of physical units, with quality flags used to exclude unstable or biased measurements. The paper highlights the importance of using checklists, considering illumination conditions and conducting test flights for the detection of potential pitfalls. Conclusion: Multi-sensor campaigns require careful planning not to jeopardise the success of the campaigns. This paper provides practical information on how to combine different UAV-mounted optical sensors and discuss the proven scientific practices for image data acquisition and post-processing in the context of crop stress monitoring.