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Mr. Tomas Vyhnanek
Mendel University in Brno, Faculty of AgriSciences

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0 Plant Biology
0 Plant Breeding
0 Plant Genetics
0 Plant biotechnology

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Journal article
Published: 01 August 2020 in Land
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Bentonite-based organic amendments may have the potential to enhance soil microbial properties. The experiment was carried out from 2014 to 2017 comprising four treatments: NPK fertilizer (nitrogen, phosphorus and potassium mineral fertilizer as a control), NPK + cattle manure, NPK + bentonite, and NPK + combination of manure with bentonite (MB) to verify this hypothesis. The effect of treatments on seven different soil microbial properties was measured: dehydrogenase activity (DHA), bacterial phospholipid fatty acid content, fungal phospholipid fatty acid content, microbial biomass carbon (Cmic), 16S rDNA, 18S rDNA, and ammonia-oxidizing bacteria in soil. The results showed that solely bentonite treatment increases the bacterial and fungal biomass, which was further confirmed by the increased 16S rDNA and 18s rDNA gene copy numbers. The only significantly decreased values upon treatment with solely bentonite were recorded for DHA and Cmic. The ammonia-oxidizing bacteria population increased with the sole application of bentonite and reached its maximum value when bentonite was applied with manure. The MB treatment showed the highest value for all seven measured properties. In summary, the application of bentonite solely might increase or decrease the soil activity, but its addition, along with manure, always promotes an abundance of soil microorganisms and their activity. The co-application of bentonite with manure altered the soil microbial properties in a 3-year field experiment in favor of increased microbial biomass, which is beneficial for agriculture and environment and reveals the potential for the restoration of polluted lands.

ACS Style

Rahul Datta; Jiri Holatko; Oldrich Latal; Tereza Hammerschmiedt; Jakub Elbl; Vaclav Pecina; Antonin Kintl; Ludmila Balakova; Maja Radziemska; Tivadar Baltazar; Petr Skarpa; Subhan Danish; Muhammad Zafar-Ul-Hye; Tomas Vyhnanek; Martin Brtnicky. Bentonite-Based Organic Amendment Enriches Microbial Activity in Agricultural Soils. Land 2020, 9, 258 .

AMA Style

Rahul Datta, Jiri Holatko, Oldrich Latal, Tereza Hammerschmiedt, Jakub Elbl, Vaclav Pecina, Antonin Kintl, Ludmila Balakova, Maja Radziemska, Tivadar Baltazar, Petr Skarpa, Subhan Danish, Muhammad Zafar-Ul-Hye, Tomas Vyhnanek, Martin Brtnicky. Bentonite-Based Organic Amendment Enriches Microbial Activity in Agricultural Soils. Land. 2020; 9 (8):258.

Chicago/Turabian Style

Rahul Datta; Jiri Holatko; Oldrich Latal; Tereza Hammerschmiedt; Jakub Elbl; Vaclav Pecina; Antonin Kintl; Ludmila Balakova; Maja Radziemska; Tivadar Baltazar; Petr Skarpa; Subhan Danish; Muhammad Zafar-Ul-Hye; Tomas Vyhnanek; Martin Brtnicky. 2020. "Bentonite-Based Organic Amendment Enriches Microbial Activity in Agricultural Soils." Land 9, no. 8: 258.

Journal article
Published: 28 June 2020 in Applied Sciences
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Rice (Oryza sativa L.) is productively affected by different environmental factors, including biotic and abiotic stress. The objectives of this research were to evaluate the genetic distinction among Kurdish rice genotypes using the simple sequence repeats (SSRs) molecular markers and to perform in vitro tests to characterize the drought tolerance of six local rice genotypes. The polymorphic information content (PIC) varied from 0.38 to 0.84 with an average of 0.56. The genetic distance ranged from 0.33 to 0.88. Drought stress had a significant impact (p ≤ 0.05) on callus growth parameters. Enzymatic antioxidant systems were predicted and exhibited a significant variation. The findings revealed that proline levels increase in proportion to polyethylene glycol (PEG) concentrations. Kalar and Gwll Swr genotypes showed the worst performances in phenotypic and biochemical traits, while Choman and Shawre exhibited the best phenotypic and biochemical performances. A positive and substantial relationship between callus fresh weight (CFW) and callus dry weight (CDW) was found under stressful and optimized conditions. Callus induction (CI) was positively and significantly associated with the catalase activity (CAT) in all stressed treatments. Based on the results for callus growth and the biochemical parameters under stress conditions, a remarkable genotype distinction, based on the tolerance reaction, was noted as follows: PEG resistant > susceptible, Choman > Shawre > White Bazyan > Red Bazyan > Gwll Swr > Kalar. The CI and CAT characteristics were considered as reliable predictors of drought tolerance in rice genotypes.

ACS Style

Didar Rahim; Petr Kalousek; Nawroz Tahir; Tomáš Vyhnánek; Petr Tarkowski; Vaclav Trojan; Dana Abdulkhaleq; Ahmad Hama Ameen; Ladislav Havel. In Vitro Assessment of Kurdish Rice Genotypes in Response to PEG-Induced Drought Stress. Applied Sciences 2020, 10, 4471 .

AMA Style

Didar Rahim, Petr Kalousek, Nawroz Tahir, Tomáš Vyhnánek, Petr Tarkowski, Vaclav Trojan, Dana Abdulkhaleq, Ahmad Hama Ameen, Ladislav Havel. In Vitro Assessment of Kurdish Rice Genotypes in Response to PEG-Induced Drought Stress. Applied Sciences. 2020; 10 (13):4471.

Chicago/Turabian Style

Didar Rahim; Petr Kalousek; Nawroz Tahir; Tomáš Vyhnánek; Petr Tarkowski; Vaclav Trojan; Dana Abdulkhaleq; Ahmad Hama Ameen; Ladislav Havel. 2020. "In Vitro Assessment of Kurdish Rice Genotypes in Response to PEG-Induced Drought Stress." Applied Sciences 10, no. 13: 4471.

Journal article
Published: 20 June 2020 in Plant Science
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Our study aimed to optimize a selection of a suitable combination of SSRs (Simple Sequence Repeats) for determination of technical Cannabis cultivars and genetic variability level. We used sequences of 23 published SSR families (107 alleles) and amplified them in 28 cultivars. One of the alleles possesses no selective information (SSR family CAN1660) due to its presence in every single tested cultivar. We excluded it, together with another 11 least informative alleles. After data filtration, we used 96 alleles to do recursive sub-sampling of random alleles' sets. We found a minimal set of 8 alleles (in three different combinations) to distinguish 28 analyzed cultivars from each other. Our results contribute to saving resources and to reduce the performance time of the molecular analysis.

ACS Style

Tomáš Vyhnánek; Eva Nevrtalová; Marie Bjelková; Barbora Balgová. SSR loci survey of technical hemp cultivars: The optimization of a cost-effective analyses to study genetic variability. Plant Science 2020, 298, 110551 .

AMA Style

Tomáš Vyhnánek, Eva Nevrtalová, Marie Bjelková, Barbora Balgová. SSR loci survey of technical hemp cultivars: The optimization of a cost-effective analyses to study genetic variability. Plant Science. 2020; 298 ():110551.

Chicago/Turabian Style

Tomáš Vyhnánek; Eva Nevrtalová; Marie Bjelková; Barbora Balgová. 2020. "SSR loci survey of technical hemp cultivars: The optimization of a cost-effective analyses to study genetic variability." Plant Science 298, no. : 110551.

Journal article
Published: 12 November 2019 in Agronomy
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Biochar application to the soil has been recommended as a carbon (C) management approach to sequester C and improve soil quality. Three-year experiments were conducted to investigate the interactive effects of three types of amendments on microbial biomass carbon, soil dehydrogenase activity and soil microbial community abundance in luvisols of arable land in the Czech Republic. Four different treatments were studied, which were, only NPK as a control, NPK + cattle manure, NPK + biochar and NPK + combination of manure with biochar. The results demonstrate that all amendments were effective in increasing the fungal and bacterial biomass, as is evident from the increased values of bacterial and fungal phospholipid fatty acid analysis. The ammonia-oxidizing bacteria population increases with the application of biochar, and it reaches its maximum value when biochar is applied in combination with manure. The overall results suggest that co-application of biochar with manure changes soil properties in favor of increased microbial biomass. It was confirmed that the application of biochar might increase or decrease soil activity, but its addition, along with manure, always promotes microbial abundance and their activity. The obtained results can be used in the planning and execution of the biochar-based soil amendments.

ACS Style

Martin Brtnicky; Tereza Dokulilova; Jiri Holatko; Vaclav Pecina; Antonin Kintl; Oldrich Latal; Tomas Vyhnanek; Jitka Prichystalova; Rahul Datta. Long-Term Effects of Biochar-Based Organic Amendments on Soil Microbial Parameters. Agronomy 2019, 9, 747 .

AMA Style

Martin Brtnicky, Tereza Dokulilova, Jiri Holatko, Vaclav Pecina, Antonin Kintl, Oldrich Latal, Tomas Vyhnanek, Jitka Prichystalova, Rahul Datta. Long-Term Effects of Biochar-Based Organic Amendments on Soil Microbial Parameters. Agronomy. 2019; 9 (11):747.

Chicago/Turabian Style

Martin Brtnicky; Tereza Dokulilova; Jiri Holatko; Vaclav Pecina; Antonin Kintl; Oldrich Latal; Tomas Vyhnanek; Jitka Prichystalova; Rahul Datta. 2019. "Long-Term Effects of Biochar-Based Organic Amendments on Soil Microbial Parameters." Agronomy 9, no. 11: 747.

Journal article
Published: 01 September 2018 in Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
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ACS Style

Jan Zloch; Magdalena Daria Vaverková; Dana Adamcová; Maja Radziemska; Tomáš Vyhnánek; Václav Trojan; Biljana Đorđević; Martin Brtnický. Seasonal Changes and Toxic Potency of Landfill Leachate for White Mustard (Sinapis alba L.). Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 2018, 66, 235 -242.

AMA Style

Jan Zloch, Magdalena Daria Vaverková, Dana Adamcová, Maja Radziemska, Tomáš Vyhnánek, Václav Trojan, Biljana Đorđević, Martin Brtnický. Seasonal Changes and Toxic Potency of Landfill Leachate for White Mustard (Sinapis alba L.). Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis. 2018; 66 (1):235-242.

Chicago/Turabian Style

Jan Zloch; Magdalena Daria Vaverková; Dana Adamcová; Maja Radziemska; Tomáš Vyhnánek; Václav Trojan; Biljana Đorđević; Martin Brtnický. 2018. "Seasonal Changes and Toxic Potency of Landfill Leachate for White Mustard (Sinapis alba L.)." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 66, no. 1: 235-242.

Journal article
Published: 31 August 2018 in Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
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ACS Style

Tomáš Vyhnánek; Pavel Hanáček; Ivana Šafránková; Biljana Đorđević; Helena Beranová; Václav Trojan; Ladislav Havel. Molecular Detection of Fungi in Paprika, Chili Powder and Black Pepper. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 2018, 66, 927 -937.

AMA Style

Tomáš Vyhnánek, Pavel Hanáček, Ivana Šafránková, Biljana Đorđević, Helena Beranová, Václav Trojan, Ladislav Havel. Molecular Detection of Fungi in Paprika, Chili Powder and Black Pepper. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis. 2018; 66 (4):927-937.

Chicago/Turabian Style

Tomáš Vyhnánek; Pavel Hanáček; Ivana Šafránková; Biljana Đorđević; Helena Beranová; Václav Trojan; Ladislav Havel. 2018. "Molecular Detection of Fungi in Paprika, Chili Powder and Black Pepper." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 66, no. 4: 927-937.

Journal article
Published: 31 August 2018 in Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
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ACS Style

Marie Grulichová; Peter Mendel; Václav Trojan; Tomáš Vyhnánek. Determination of Chlorophyll and Carotenoids Present in the Seeds of the Plant Family Apiaceae and the Impact on Seed Quality. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 2018, 66, 859 -869.

AMA Style

Marie Grulichová, Peter Mendel, Václav Trojan, Tomáš Vyhnánek. Determination of Chlorophyll and Carotenoids Present in the Seeds of the Plant Family Apiaceae and the Impact on Seed Quality. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis. 2018; 66 (4):859-869.

Chicago/Turabian Style

Marie Grulichová; Peter Mendel; Václav Trojan; Tomáš Vyhnánek. 2018. "Determination of Chlorophyll and Carotenoids Present in the Seeds of the Plant Family Apiaceae and the Impact on Seed Quality." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 66, no. 4: 859-869.

Journal article
Published: 28 June 2018 in Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
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ACS Style

Martin Král; Jana Pokorná; Bohuslava Tremlová; Martina Ošťádalová; Václav Trojan; Tomáš Vyhnánek; Maria Walczycka. Colored Wheat: Anthocyanin Content, Grain Firmness, Dough Properties, Bun Texture Profile. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 2018, 66, 685 -690.

AMA Style

Martin Král, Jana Pokorná, Bohuslava Tremlová, Martina Ošťádalová, Václav Trojan, Tomáš Vyhnánek, Maria Walczycka. Colored Wheat: Anthocyanin Content, Grain Firmness, Dough Properties, Bun Texture Profile. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis. 2018; 66 (3):685-690.

Chicago/Turabian Style

Martin Král; Jana Pokorná; Bohuslava Tremlová; Martina Ošťádalová; Václav Trojan; Tomáš Vyhnánek; Maria Walczycka. 2018. "Colored Wheat: Anthocyanin Content, Grain Firmness, Dough Properties, Bun Texture Profile." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 66, no. 3: 685-690.

Journal article
Published: 02 February 2018 in Potravinarstvo Slovak Journal of Food Sciences
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ACS Style

Ondřej Šťastník; Eva Mrkvicová; Leoš Pavlata; Andrea Roztočilová; Miroslav Jůzl; Roman Pytel; Tomáš Vyhnánek; Petr Martinek. Influence of feeding colored wheat varieties on selected quality parameters of broiler chicken’s meat. Potravinarstvo Slovak Journal of Food Sciences 2018, 12, 1 .

AMA Style

Ondřej Šťastník, Eva Mrkvicová, Leoš Pavlata, Andrea Roztočilová, Miroslav Jůzl, Roman Pytel, Tomáš Vyhnánek, Petr Martinek. Influence of feeding colored wheat varieties on selected quality parameters of broiler chicken’s meat. Potravinarstvo Slovak Journal of Food Sciences. 2018; 12 (1):1.

Chicago/Turabian Style

Ondřej Šťastník; Eva Mrkvicová; Leoš Pavlata; Andrea Roztočilová; Miroslav Jůzl; Roman Pytel; Tomáš Vyhnánek; Petr Martinek. 2018. "Influence of feeding colored wheat varieties on selected quality parameters of broiler chicken’s meat." Potravinarstvo Slovak Journal of Food Sciences 12, no. 1: 1.

Original paper
Published: 28 August 2017 in Waste and Biomass Valorization
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Landfill leachate is one of the major sources of pollutions discharged into the environment. It is composed from a complex mixture of chemicals and handling typically involves treatment either on-site or at a wastewater treatment plants but phytoremediation is a promising method. The aim of this work was to evaluate the potential of agronomic plant species with high annual biomass yield (Cannabis sativa L.) for toxicity removal from landfill leachate. Raw leachate collected from the pond of untreated leachate at sanitary landfill in Czech Republic was used in the study. The hemp cultivation experiments were performed in the beginning of 2017 under laboratory conditions using three hemp cultivars registered in the European Union: Tiborszállási (Hungary), Bialobrzeska (Poland) and Monoica (Hungary). The seeds were used for modified standard mustard germination test. The germination of hemp cultivars was tested using the hydroponics medium supplemented with leachate 25, 50, 75, 90 and 100%. The control seeds were growing on untreated nutrient medium under the same condition. The nature of germination of seeds was studied. Based on the obtained results, it can be concluded that the tested samples of leachate were toxic for hemp cultivars (C. sativa L.). Growth inhibition (%) at the studied samples ranged from −6.48 to 75.78%.

ACS Style

Magdalena Daria Vaverková; Jan Zloch; Dana Adamcová; Maja Radziemska; Tomáš Vyhnánek; Václav Trojan; Jan Winkler; Biljana Đorđević; Jakub Elbl; Martin Brtnický. Landfill Leachate Effects on Germination and Seedling Growth of Hemp Cultivars (Cannabis Sativa L.). Waste and Biomass Valorization 2017, 10, 369 -376.

AMA Style

Magdalena Daria Vaverková, Jan Zloch, Dana Adamcová, Maja Radziemska, Tomáš Vyhnánek, Václav Trojan, Jan Winkler, Biljana Đorđević, Jakub Elbl, Martin Brtnický. Landfill Leachate Effects on Germination and Seedling Growth of Hemp Cultivars (Cannabis Sativa L.). Waste and Biomass Valorization. 2017; 10 (2):369-376.

Chicago/Turabian Style

Magdalena Daria Vaverková; Jan Zloch; Dana Adamcová; Maja Radziemska; Tomáš Vyhnánek; Václav Trojan; Jan Winkler; Biljana Đorđević; Jakub Elbl; Martin Brtnický. 2017. "Landfill Leachate Effects on Germination and Seedling Growth of Hemp Cultivars (Cannabis Sativa L.)." Waste and Biomass Valorization 10, no. 2: 369-376.

Journal article
Published: 01 March 2017 in Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
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ACS Style

Lenka Machálková; Marie Janečková; Luděk Hřivna; Yvona Dostálová; Joany Hernandez; Eva Mrkvicova; Tomas Vyhnanek; Václav Trojan. Impact of Added Colored Wheat Bran on Bread Quality. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 2017, 65, 99 -104.

AMA Style

Lenka Machálková, Marie Janečková, Luděk Hřivna, Yvona Dostálová, Joany Hernandez, Eva Mrkvicova, Tomas Vyhnanek, Václav Trojan. Impact of Added Colored Wheat Bran on Bread Quality. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis. 2017; 65 (1):99-104.

Chicago/Turabian Style

Lenka Machálková; Marie Janečková; Luděk Hřivna; Yvona Dostálová; Joany Hernandez; Eva Mrkvicova; Tomas Vyhnanek; Václav Trojan. 2017. "Impact of Added Colored Wheat Bran on Bread Quality." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 65, no. 1: 99-104.

Journal article
Published: 20 January 2017 in Potravinarstvo Slovak Journal of Food Sciences
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ACS Style

Ondřej Šťastník; Filip Karásek; Miroslav Jůzl; Eva Mrkvicová; Leoš Pavlata; Šárka Nedomová; Václav Trojan; Tomáš Vyhnánek; Petr Doležal; Petr Martinek. The effect of feeding wheat varieties with different grain pigmentation on growth performance, texture, colour and meat sensory traits of broiler chickens. Potravinarstvo Slovak Journal of Food Sciences 2017, 11, 1 .

AMA Style

Ondřej Šťastník, Filip Karásek, Miroslav Jůzl, Eva Mrkvicová, Leoš Pavlata, Šárka Nedomová, Václav Trojan, Tomáš Vyhnánek, Petr Doležal, Petr Martinek. The effect of feeding wheat varieties with different grain pigmentation on growth performance, texture, colour and meat sensory traits of broiler chickens. Potravinarstvo Slovak Journal of Food Sciences. 2017; 11 (1):1.

Chicago/Turabian Style

Ondřej Šťastník; Filip Karásek; Miroslav Jůzl; Eva Mrkvicová; Leoš Pavlata; Šárka Nedomová; Václav Trojan; Tomáš Vyhnánek; Petr Doležal; Petr Martinek. 2017. "The effect of feeding wheat varieties with different grain pigmentation on growth performance, texture, colour and meat sensory traits of broiler chickens." Potravinarstvo Slovak Journal of Food Sciences 11, no. 1: 1.

Original article
Published: 22 December 2016 in Trees
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Species ofAesculusgenus are divided into five main sections according to their susceptibility and resistance to horse chestnut leaf miner using molecular markers. Some species of the genus Aesculus are yearly severely infested by horse chestnut leaf miner (Cameraria ohridella). The larvae mine the leaves and under appropriate conditions can damage up to 100% of the leaf area. In this study, we assessed the genetic diversity of eight species of horse chestnut and their genotypes which have varying susceptibility to the leaf miner. Analysis of eight microsatellite loci showed high polymorphic information content 0.45–0.77 (0.60 in average) and high genetic diversity. For each locus, we found on average 5.50 alleles. During three vegetation periods, the leaf area damage of these genotypes was evaluated using the statistical software Assess 2.0. Different pressure of leaf miner and different development at each locality was recorded, and in some cases less damage was found, in one case the lesser damage was permanent. From the data, a pathological scale was established and a dendogram of similarity was created. Based on SSR analysis, four groups of Aesculus trees were distinguished according to their resistance or susceptibility and five main section+Hybrid were found. By cloning and analysing the inner transcribed spacers, the ITS1 and ITS2, inner and inter species variability was examined. For the sequence of hybrid species A. × carnea, no compliance was revealed in NBCI database. For this reason, the first sequence of this genotype was obtained.

ACS Style

Václav Bačovský; Tomas Vyhnanek; Pavel Hanáček; Josef Mertelík; Ivana Šafránková. Genetic diversity of chestnut tree in relation to susceptibility to leaf miner (Cameraria ohridella Deschka & Dimič). Trees 2016, 31, 753 -763.

AMA Style

Václav Bačovský, Tomas Vyhnanek, Pavel Hanáček, Josef Mertelík, Ivana Šafránková. Genetic diversity of chestnut tree in relation to susceptibility to leaf miner (Cameraria ohridella Deschka & Dimič). Trees. 2016; 31 (2):753-763.

Chicago/Turabian Style

Václav Bačovský; Tomas Vyhnanek; Pavel Hanáček; Josef Mertelík; Ivana Šafránková. 2016. "Genetic diversity of chestnut tree in relation to susceptibility to leaf miner (Cameraria ohridella Deschka & Dimič)." Trees 31, no. 2: 753-763.

Journal article
Published: 12 August 2016 in Horticultural Science
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Open Access Agricultural Journals published by Institute of Agricultural Economics and Information

ACS Style

H. Fišerová; T. Vyhnánek; Z. Staňková; V. Kozák; M. Klemš; L. Havel. Effects of garlic genotype on cloves formation under in vitro conditions. Horticultural Science 2016, 43, 142 -148.

AMA Style

H. Fišerová, T. Vyhnánek, Z. Staňková, V. Kozák, M. Klemš, L. Havel. Effects of garlic genotype on cloves formation under in vitro conditions. Horticultural Science. 2016; 43 (No. 3):142-148.

Chicago/Turabian Style

H. Fišerová; T. Vyhnánek; Z. Staňková; V. Kozák; M. Klemš; L. Havel. 2016. "Effects of garlic genotype on cloves formation under in vitro conditions." Horticultural Science 43, no. No. 3: 142-148.

Journal article
Published: 28 June 2016 in Journal of Consumer Protection and Food Safety
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Delivery of foreign DNA is still a very controversial issue, especially in relation to genetically modified organisms. It is not necessary to use genetically modified feed to verify the possible transfer of DNA from food into the body. In this study, we evaluated the possible transfer of DNA from wheat forage (of single component diet) into the blood of three different organisms. DNA isolation kits were used to isolate total DNA from blood and feed. Blood was taken for analysis from rats (after 23 days feeding wheat), broilers (15 days), and carp (34 days). Housekeeping genes were used to verify blood samples (rats: glyceraldehyde-3-phosphate dehydrogenase/GAPDH/, broilers: growth hormone gene/GCH/and carp: small ribosomal subunit/40S/). We used GAPDH and actin to determine whether DNA from wheat was transferred into animal blood. No purple and common wheat DNA fragments were detected in the blood of any animal tested, suggesting no transfer of the tested wheat DNA into the body. Various contents of anthocyanins in the feeding variants had no effect on the transfer of wheat DNA into the blood of animals. Our results correspond to some studies that have not proved transfer DNA from genetically modified feed into the target organism.

ACS Style

Václav Trojan; Tomas Vyhnanek; Ondřej Šťastník; Eva Mrkvicová; Jan Mareš; Ladislav Havel. Detection of DNA fragments from wheat in blood of animals. Journal of Consumer Protection and Food Safety 2016, 11, 259 -264.

AMA Style

Václav Trojan, Tomas Vyhnanek, Ondřej Šťastník, Eva Mrkvicová, Jan Mareš, Ladislav Havel. Detection of DNA fragments from wheat in blood of animals. Journal of Consumer Protection and Food Safety. 2016; 11 (3):259-264.

Chicago/Turabian Style

Václav Trojan; Tomas Vyhnanek; Ondřej Šťastník; Eva Mrkvicová; Jan Mareš; Ladislav Havel. 2016. "Detection of DNA fragments from wheat in blood of animals." Journal of Consumer Protection and Food Safety 11, no. 3: 259-264.

Journal article
Published: 07 June 2016 in Journal of Food Safety
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Dried red pepper is one of the most commonly used spices in many parts of the world. In this study molecular biology methods were applied for detection of contamination in nine samples of paprika powder. Internal Transcribed Spacer (ITS) regions were selected for sequencing as they have a high variability between species and organisms and therefore are an appropriate tool for taxonomic identification. The sequence analysis of the ITS regions were identified by high sequence similarity with the ITS regions of many microscopic fungi, especially representatives of the class Ascomycota and several other yeast species. This work was a qualitative rather than a quantitative detection, so the extent to which fungi and yeasts were present in the samples is unknown. However, supplied quality certificates (microbiological data) indicated the overall quality of samples. Harmful microorganisms were identified in the paprika powder samples and the likely mode of contamination was identified. The presence of undesirable pathogens can be a serious problem for consumers, especially contamination by microscopic fungi that have the potential to produce mycotoxins. Today's conventional methods for determining contamination by microorganisms are time consuming and can be difficult to identify some less common types of pathogens. Fungal DNA (ITS regions) sequences of the class Ascomycetes were identified from samples of sweet and hot paprika powder.

ACS Style

Tomáš Vyhnánek; Zuzana Machálková; Václav Trojan; Pavel Hanáček; Ivana Šafránková; Ladislav Havel. Qualitative Detection of Fungal Contamination in Paprika Powder. Journal of Food Safety 2016, 37, e12296 .

AMA Style

Tomáš Vyhnánek, Zuzana Machálková, Václav Trojan, Pavel Hanáček, Ivana Šafránková, Ladislav Havel. Qualitative Detection of Fungal Contamination in Paprika Powder. Journal of Food Safety. 2016; 37 (1):e12296.

Chicago/Turabian Style

Tomáš Vyhnánek; Zuzana Machálková; Václav Trojan; Pavel Hanáček; Ivana Šafránková; Ladislav Havel. 2016. "Qualitative Detection of Fungal Contamination in Paprika Powder." Journal of Food Safety 37, no. 1: e12296.

Journal article
Published: 01 January 2016 in Acta Veterinaria Brno
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The influence of anthocyanins on antioxidant activity and liver function was measured in three different animal species (rats, chickens and fish). Purple Konini wheat with the total anthocyanin content (TAC) of 41.70 mg/kg was fed to the experimental groups of animals (rats n = 32; chickens n = 32; fish n = 20). The same numbers of animals (control groups) were fed common wheat with TAC of 24.95 mg/kg. At the end of the experiment, the animals were killed and blood and liver tissues were taken for biochemical analyses. The liver enzyme activities were measured in blood. Antioxidant activity in the liver tissue was measured using the DPPH (2,2-diphenyl-1-picrylhydrazyl test), FR (Free Radicals method), FRAP (Ferric Reducing Antioxidant Power method), and ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) test. Significantly higher values (P< 0.05) measured by the DPPH and FR methods were determined in the liver from rats fed Konini wheat. The chickens fed Konini wheat showed significantly different values using DPPH, FR and ABTS methods. No significant differences were found in hepatopancreases of fish. Animals fed Konini wheat showed a tendency to lower liver enzyme activities in general, but a significant difference was found only in gamma-glutamyl transferase activity in the chicken’s blood. Our results suggest that feeding a higher content of anthocyanins can improve the antioxidant activity and function of the liver tissue.

ACS Style

Eva Mrkvicova; Leos Pavlata; Filip Karásek; Ondřej Šťastník; Eva Doležalová; Václav Trojan; Tomas Vyhnanek; Luděk Hřivna; Veronika Holeksová; Jan Mareš; Tomáš Brabec; Pavel Horký; Branislav Ruttkay-Nedecký; Vojtech Adam; René Kizek. The influence of feeding purple wheat with higher content of anthocyanins on antioxidant status and selected enzyme activity of animals. Acta Veterinaria Brno 2016, 85, 371 -376.

AMA Style

Eva Mrkvicova, Leos Pavlata, Filip Karásek, Ondřej Šťastník, Eva Doležalová, Václav Trojan, Tomas Vyhnanek, Luděk Hřivna, Veronika Holeksová, Jan Mareš, Tomáš Brabec, Pavel Horký, Branislav Ruttkay-Nedecký, Vojtech Adam, René Kizek. The influence of feeding purple wheat with higher content of anthocyanins on antioxidant status and selected enzyme activity of animals. Acta Veterinaria Brno. 2016; 85 (4):371-376.

Chicago/Turabian Style

Eva Mrkvicova; Leos Pavlata; Filip Karásek; Ondřej Šťastník; Eva Doležalová; Václav Trojan; Tomas Vyhnanek; Luděk Hřivna; Veronika Holeksová; Jan Mareš; Tomáš Brabec; Pavel Horký; Branislav Ruttkay-Nedecký; Vojtech Adam; René Kizek. 2016. "The influence of feeding purple wheat with higher content of anthocyanins on antioxidant status and selected enzyme activity of animals." Acta Veterinaria Brno 85, no. 4: 371-376.

Journal article
Published: 01 January 2016 in Food Technology and Biotechnology
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Grain hardness is one of the most important quality characteristics of wheat (Triticum aestivum L.). It is a significant property of wheat grains and relates to milling quality and end product quality. Grain hardness is caused by the presence of puroindoline genes (Pina and Pinb). A collection of 25 genotypes of wheat with unusual grain colour (blue aleurone, purple and white pericarp, yellow endosperm) was studied by polymerase chain reaction (PCR) for the diversity within Pina and Pinb (alleles: Pina-D1a, Pina-D1b, Pinb-D1a, Pinb- -D1b, Pinb-D1c and Pinb-D1d). The endosperm structure was determined by a non-destructive method using light transflectance meter and grain hardness by a texture analyser. Genotype Novosibirskaya 67 and isogenic ANK lines revealed hitherto unknown alleles at the locus for the annealing of primers of Pinb-D1. Allele Pinb-D1c was found to be absent from each genotype. The mealy endosperm ranged from 0 to 100% and grain hardness from 15.10 to 26.87 N per sample.

ACS Style

Mária Presinszká; Klára Štiasna; Tomáš Vyhnánek; Vaclav Trojan; Eva Mrkvicová; Luděk Hřivna; Ladislav Havel. Identification of Alleles of Puroindoline Genes and Their Effect on Wheat (Triticum aestivum L.) Grain Texture. Food Technology and Biotechnology 2016, 54, 103 -107.

AMA Style

Mária Presinszká, Klára Štiasna, Tomáš Vyhnánek, Vaclav Trojan, Eva Mrkvicová, Luděk Hřivna, Ladislav Havel. Identification of Alleles of Puroindoline Genes and Their Effect on Wheat (Triticum aestivum L.) Grain Texture. Food Technology and Biotechnology. 2016; 54 (1):103-107.

Chicago/Turabian Style

Mária Presinszká; Klára Štiasna; Tomáš Vyhnánek; Vaclav Trojan; Eva Mrkvicová; Luděk Hřivna; Ladislav Havel. 2016. "Identification of Alleles of Puroindoline Genes and Their Effect on Wheat (Triticum aestivum L.) Grain Texture." Food Technology and Biotechnology 54, no. 1: 103-107.

Journal article
Published: 17 December 2015 in Potravinarstvo Slovak Journal of Food Sciences
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Tomáš Vyhnánek; Vaclav Trojan; Klára Štiasna; Mária Presinszká; Eva Mrkvicová; Luděk Hřivna; Ladislav Havel. Testing of DNA isolation for the identification of hemp. Potravinarstvo Slovak Journal of Food Sciences 2015, 9, 1 .

AMA Style

Tomáš Vyhnánek, Vaclav Trojan, Klára Štiasna, Mária Presinszká, Eva Mrkvicová, Luděk Hřivna, Ladislav Havel. Testing of DNA isolation for the identification of hemp. Potravinarstvo Slovak Journal of Food Sciences. 2015; 9 (1):1.

Chicago/Turabian Style

Tomáš Vyhnánek; Vaclav Trojan; Klára Štiasna; Mária Presinszká; Eva Mrkvicová; Luděk Hřivna; Ladislav Havel. 2015. "Testing of DNA isolation for the identification of hemp." Potravinarstvo Slovak Journal of Food Sciences 9, no. 1: 1.

Journal article
Published: 01 August 2015 in Potravinarstvo Slovak Journal of Food Sciences
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This study assessed and compared the influence of feeding wheat with purple pericarp (variety Konini) and standard coloured wheat (red variety Bohemia) on the growth characteristics of fingerling carp (Cyprinus carpio L.) of the "Amurský lysec" line. The total content of anthocyanins converted to cyanidin 3-glucoside in the control Bohemia wheat was 24.95 mg.kg-1 and in the Konini purple wheat 41.70 mg.kg-1. Two experimental variants for feed were evaluated: dipped wheat grain and crushed wheat grain. The feed dose for wheat was 1.5% of the fish stock weight and for natural food (frozen Chironomid larvae) was 0.2% of fish stock weight to all variants. Growth parameters (body length, body weight, Fulton's condition factor and feed conversion ratio) of the fish were evaluated after one month of administration. The feed consumption and physico-chemical parameters (temperature, oxygen saturation, pH, N-NH4 +, N-NO2-, N-NO3- and Cl-) of the environment were observed. During the feeding test, no major differences in food consumption among variations feeding on either wheat and on Chironomid larvae were noted. Satisfying results for mas and length gain were achieved in V2 wheat with purple pericarp (Konini variety - dipped grain), where the average total body length was 156.56 mm and the average unit mass was 60.81 g. In this variant, higher values of the parameters were achieved compared to the control group (100.6%, resp. 104.2%). A positive impact of wheat with purple pericarp on the evaluated parameter of fish condition factor was demonstrated. This trend was confirmed in all variants. No effect was demonstrated for mechanical disruption of kernels on the level of utilization of nutrients. In further experiments on growth characteristics we would like to determine antioxidant parameters in the blood and liver of fry.