Biosensors are transforming point-of-care diagnostics by simplifying the detection process and enabling rapid, accurate testing. This study introduces a novel, reusable biosensor designed for direct viral RNA detection from unfiltered saliva, targeting SARS-CoV-2. Unlike conventional methods requiring filtration, our biosensor leverages a unique electrode design that prevents interference from saliva debris, allowing precise measurements. The biosensor is based on electrochemical principles, employing oligonucleotide probes immobilized on a hydrophobic-coated electrode, which prevents air bubbles and salt crystal formation. During validation, the biosensor demonstrated a sensitivity and specificity of 100%, accurately identifying SARS-CoV-2 in saliva samples without false positives or negatives. Cross-validation with RT-qPCR, the gold standard for COVID-19 diagnostics, confirmed the reliability of our device. The biosensor’s performance was tested on 60 participants, yielding 12 true positive results and 48 true negatives, aligning perfectly with RT-qPCR outcomes. This reusable, easy-to-use biosensor offers significant potential for point-of-care applications in various healthcare settings, providing a fast, efficient, and cost-effective method for detecting viral infections such as COVID-19. Its robust design, minimal sample preparation requirements, and multiple-use capability mark a significant advancement in biosensing technology.
In the paper the method and results are presented of the testing of tribological performance of a number of hard materials available commercially. The tests consisted in unidirectional sliding with liquid lubrication. The load and velocity regime chosen were similar to a standard four-ball lubricity test with constant velocity and load increasing over time. The regime was modified in such a way that over the initial part of the test both the velocity and load were linearly increased until a certain stable level of each input parameter was reached and the test was then continued till the termination due to chosen criteria. The materials used were high alloy tool steels and sintered carbides, normally used in working of metals by cutting. The lubricants environmentally inert or friendly fluids: water, mineral oil-in-water emulsion and vegetable oil. In the tests, typical sliding friction parameters were recorded, later evaluated on the basis of multiple parameters observable within the test such as e.g. maximum load and velocity reached, calculated maximum surface pressure or presence and amplitude of friction induced vibrations. In the group of material/material/lubricant combinations a ranking was created for each parameter in the assessment set by awarding points for performance. Best performance was granted the least amount of points. Overall performance was then judged by the accumulated number of points. The materials, which were evaluated as performing best have been chosen as input materials for the design and manufacture of a prototype motor for later laboratory and field testing.
Fiber optic sensors have gained popularity over the last few decades. This is due to their numerous advantages, such as good metrological parameters, biocompatibility and resistance to magnetic and electric fields and environmental pollution. However, those built from glass fiber have one main disadvantage—they are fragile, meaning they can be easily damaged, even by the presence of vibration. Due to the great progress made by material research recently, it is possible to build such a sensor with polymer fibers instead. Although those fibers have worse transmission parameters compared to telecommunication fibers, they provide the possibility to realize flexible fiber optic sensors. Taking into consideration other advantages of such fibers, including biocompatibility, electromagnetic resistance and even, biodegradation characteristics, as well as there being a variety of materials we can use, it can be seen that those materials are beneficial to produce fiber optic sensors. This paper aims to provide researchers with guidelines on the factors to consider when choosing a material for bent fiber optic sensors, depending on the application.
The titanium and its alloys can be subjected to surface treatment, including laser treatment. In this work a new laser treatment at cryogenic conditions of Ti6Al4V alloy has been described. The work has been aimed at establishing whether such surface treatment could be suitable for implants working under wear in biological corrosive environment. The remelting has been made with the use of CO2 continuous work laser at laser power between 3 and 6 kW, at scan rate 0.5 and 1 m/s. The microstructure, surface topography, hardness, microhardness and wear linear rate and mass loss under tribological tests made in Ringer`s solution have been made. The results have shown that despite the surface cracking the tribological properties in simulated body fluid have been substantially improved.
In this paper, results of the experimental study on improving wear resistance in sliding friction of Al-based alloy are presented. The technique used involves the formation of a metal matrix composite (MMC) in the alloy surface layer by laser dispersion of carbide powders such as WC, TiC and SiC. For WC and TiC MMC surface coatings fabricated under conditions typical for most of the technologically relevant solid-state lasers (wavelength range of 0.8–1.1 μm), the nearly inversely proportional dependence of the required laser energy density on the powder mass density is observed. Highly homogenous distribution of powder particle content (up to 40%) in the MMC surface coatings of a thickness between 0.8 and 1.6 mm obtained by multiple scanning is observed in the cross-section of specimens processed within a rather narrow parameter window. Tribological tests and comparison to untreated material reveal wear resistance increases by five- and ten-fold, observed in samples with laser-dispersed TiC and WC powders, respectively. Results indicate that substantial modification and reinforcement of the surface layer can be achieved in Al alloy in a one-step process without substrate preheating.
The paper presents the result of tribological test of ABS and steel samples sliding under dry friction. Polymeric samples were manufactured of ABS material using FDM technology. Testing was carried out in unidirectional sliding in a ring-on-flat contact in a PT-3 tribometer. The scope of tested parameters included volumetric and mass wear, the friction coefficient, and polymeric specimen temperature. Polymeric specimens used in the study were manufactured at various settings of the 3D printing process such as the orientation of the specimen in print with respect to the printer building tray and the thickness of a single layer of the deposited material. Comparisons of the impact of these parameters on tribological performance of the sliding contact were analysed.