The presence of heavy metals in waste water brings serious environmental pollution that threatens human health and the ecosystem. Bioremediation of heavy metals has received considerable and growing interest over the years. Thus, this paper presents the use of the Spent Mushroom Substrate (SMS) of Agaricus bisporus cultivation as a bioremediating agent to remove heavy metals that are present in industrial waters. These metals include chromium, lead, iron, cobalt, nickel, manganese, zinc, copper and aluminium. In particular, this study analyses the performance of SMS bioreactors with different groups of heavy metals at various concentrations. Between 80% and 98% of all contaminants that were analysed can be removed with 5 kg of SMS at hydraulic retention times of 10 and 100 days. The best removal efficiencies and longevities were achieved when removing iron (III), nickel and cobalt from contaminated water at a pH of 2.5. These results suggest that SMS can successfully treat waste water that has been contaminated with heavy metals.

The purpose of this work was to analyse the compaction of a cohesive material using different Discrete Element Method (DEM) simulators to determine the equivalent contact models and to identify how some simulation parameters affect the compaction results (maximum force and compact appearance) and computational costs. For this purpose, three cohesion contact models were tested: linear cohesion in EDEM, and simplified Johnson-Kendall-Roberts (SJKR) and modified SJKR (SJKR2) in LIGGGHTS. The influence of the particle size distribution (PSD) on the results was also investigated. Further assessments were performed on the effect of (1) selecting different timesteps, (2) using distinct conversion tolerances to export the three-dimensional models to standard triangle language (STL) files, and (3) moving the punch with different speeds. Consequently, we determined that a timestep equal to a 10% Rayleigh timestep, a conversion tolerance of 0.01 mm, and a punch speed of 0.1 m/s is adequate for simulating the compaction process using the materials and the contact models in this work. The results showed that the maximum force was influenced by the PSD due to the rearrangement of the particles. The PSD was also related to the computational cost because of the number of simulated particles and their sizes. Finally, an equivalence was found between the linear cohesion and SJKR2 contact models.