This page has only limited features, please log in for full access.
Research-Professor. Director of Research Institute in Biomedical Sciences, University of Guadalajara, Mexico. Lines of research: Immunogenetics of autoimmune diseases and Nutritional genomics of human nutrition. National Researcher level 3 of the National Council of Science and Technology (CONACYT).
Developing countries have reported lower molecular diagnostic testing levels due to a lack of resources. Therefore, antibody tests represent an alternative to detect exposure to SARS-CoV-2 and analyze possible risk factors. We aimed to describe and compare the clinical-epidemiological characteristics and the quality of food intake in Mexican individuals with a positive or negative test to antibodies against SARS-CoV-2. We carried out antibody tests and applied a survey to 1799 individuals; 42% were positive, and diabetes was more prevalent in these cases (p< 0.01). No differences were identified in the blood type nor influenza vaccination between groups. Coughing, respiratory distress, muscle pain, joint pain, and anosmia were the most prevalent symptoms among seropositive cases (p< 0.0001). Food intake quality was similar in both groups, except for the most consumed type of fat (p = 0.006). In conclusion, this study supports the association of diabetes as a principal risk factor for SARS-CoV-2 infection in the Mexican population. The results do not support previous associations between blood group or influenza vaccination as protective factors against SARS-CoV-2 infection. However, frequent consumption of polyunsaturated fats is highlighted as a new possible associated factor with COVID-19, which more studies should corroborate as with all novel findings.
Gabriela Macedo-Ojeda; José Francisco Muñoz-Valle; Patricia Yokogawa-Teraoka; Andrea Carolina Machado-Sulbarán; María Guadalupe Loza-Rojas; Atziri Citlally García-Arredondo; Rafael Tejeda-Constantini; Alejandra Natali Vega-Magaña; Guillermo González-Estevez; Mariel García-Chagollán; José Sergio Zepeda-Nuño; Jorge Hernández-Bello. COVID-19 Screening by Anti-SARS-CoV-2 Antibody Seropositivity: Clinical and Epidemiological Characteristics, Comorbidities, and Food Intake Quality. International Journal of Environmental Research and Public Health 2021, 18, 8995 .
AMA StyleGabriela Macedo-Ojeda, José Francisco Muñoz-Valle, Patricia Yokogawa-Teraoka, Andrea Carolina Machado-Sulbarán, María Guadalupe Loza-Rojas, Atziri Citlally García-Arredondo, Rafael Tejeda-Constantini, Alejandra Natali Vega-Magaña, Guillermo González-Estevez, Mariel García-Chagollán, José Sergio Zepeda-Nuño, Jorge Hernández-Bello. COVID-19 Screening by Anti-SARS-CoV-2 Antibody Seropositivity: Clinical and Epidemiological Characteristics, Comorbidities, and Food Intake Quality. International Journal of Environmental Research and Public Health. 2021; 18 (17):8995.
Chicago/Turabian StyleGabriela Macedo-Ojeda; José Francisco Muñoz-Valle; Patricia Yokogawa-Teraoka; Andrea Carolina Machado-Sulbarán; María Guadalupe Loza-Rojas; Atziri Citlally García-Arredondo; Rafael Tejeda-Constantini; Alejandra Natali Vega-Magaña; Guillermo González-Estevez; Mariel García-Chagollán; José Sergio Zepeda-Nuño; Jorge Hernández-Bello. 2021. "COVID-19 Screening by Anti-SARS-CoV-2 Antibody Seropositivity: Clinical and Epidemiological Characteristics, Comorbidities, and Food Intake Quality." International Journal of Environmental Research and Public Health 18, no. 17: 8995.
Rheumatoid arthritis (RA) is an autoimmune inflammatory joint disease with complex pathogenesis associated with cytokine dysregulation. Macrophage migration inhibitory factor (MIF) plays a role in systemic inflammation and joint destruction in RA and could be associated with the secretion of other immune-modulatory cytokines such as IL-25, IL-31, and IL-33. For the above, our main aim was to evaluate the IL-25, IL-31, and IL-33 secretion from recombinant human MIF (rhMIF)-stimulated peripheral blood mononuclear cells (PBMC) of RA patients. The rhMIF and lipopolysaccharide (LPS) plus rhMIF stimuli promote the secretion of IL-25, IL-31, and IL-33 (p< 0.05) from PBMC of RA patients. The study groups, the different stimuli, and the interaction between both showed a statistically significant effect on the secretion of IL-25 (p< 0.05) and IL-31 (p< 0.01). The study of the effect of the RA patient treatments and their interaction with the effect of stimuli did not show an interaction between them. In conclusion, our study generates new evidence for the role of MIF in the secretion of IL-25, IL-31, and IL-33 and its immunomodulatory effect on RA.
Samuel García-Arellano; Luis Alexis Hernández-Palma; Sergio Cerpa-Cruz; Gabriela Athziri Sánchez-Zuno; Melva Guadalupe Herrera-Godina; José Francisco Muñoz-Valle. The Novel Role of MIF in the Secretion of IL-25, IL-31, and IL-33 from PBMC of Patients with Rheumatoid Arthritis. Molecules 2021, 26, 4968 .
AMA StyleSamuel García-Arellano, Luis Alexis Hernández-Palma, Sergio Cerpa-Cruz, Gabriela Athziri Sánchez-Zuno, Melva Guadalupe Herrera-Godina, José Francisco Muñoz-Valle. The Novel Role of MIF in the Secretion of IL-25, IL-31, and IL-33 from PBMC of Patients with Rheumatoid Arthritis. Molecules. 2021; 26 (16):4968.
Chicago/Turabian StyleSamuel García-Arellano; Luis Alexis Hernández-Palma; Sergio Cerpa-Cruz; Gabriela Athziri Sánchez-Zuno; Melva Guadalupe Herrera-Godina; José Francisco Muñoz-Valle. 2021. "The Novel Role of MIF in the Secretion of IL-25, IL-31, and IL-33 from PBMC of Patients with Rheumatoid Arthritis." Molecules 26, no. 16: 4968.
One of the micronutrients that has attracted the most attention in relation to COVID-19 is vitamin D. Although several factors affect its sufficiency; it has been argued that an optimal diet can ensure the intake of micronutrients with effects on immune response. Therefore, in this work we aimed to evaluate the food intake quality of SARS-CoV-2 positive Mexican patients and some of the common factors related to vitamin D deficiency. We conducted a cross-sectional study in 40 SARS-CoV-2 positive patients. Serum samples and clinical parameters were collected. Micronutrient intake and food intake quality were assessed with a 24-h dietary recall and the Mini-ECCA v.2, respectively. Thirty-eight percent of the sample had a healthy food intake. The median 25(OH)D concentration was 22.7 ng/mL. A considerable insufficient intake of micronutrients with immunomodulatory effects such as vitamin D (p< 0.0001), vitamin E (p< 0.0001), and zinc (p< 0.0001) was shown. Patients with 25(OH)D sufficiency, defined as a concentration >30 ng/mL, had better food intake quality (p = 0.02) and an intense physical activity (p = 0.03). In conclusion, a better level of food intake quality and intense physical activity are associated with 25(OH)D sufficiency in SARS-CoV-2 positive Mexican patients.
Guillermo González-Estevez; Francisco Turrubiates-Hernández; Laura Herrera-Jiménez; Gabriela Sánchez-Zuno; Melva Herrera-Godina; José Muñoz-Valle. Association of Food Intake Quality with Vitamin D in SARS-CoV-2 Positive Patients from Mexico: A Cross-Sectional Study. International Journal of Environmental Research and Public Health 2021, 18, 7266 .
AMA StyleGuillermo González-Estevez, Francisco Turrubiates-Hernández, Laura Herrera-Jiménez, Gabriela Sánchez-Zuno, Melva Herrera-Godina, José Muñoz-Valle. Association of Food Intake Quality with Vitamin D in SARS-CoV-2 Positive Patients from Mexico: A Cross-Sectional Study. International Journal of Environmental Research and Public Health. 2021; 18 (14):7266.
Chicago/Turabian StyleGuillermo González-Estevez; Francisco Turrubiates-Hernández; Laura Herrera-Jiménez; Gabriela Sánchez-Zuno; Melva Herrera-Godina; José Muñoz-Valle. 2021. "Association of Food Intake Quality with Vitamin D in SARS-CoV-2 Positive Patients from Mexico: A Cross-Sectional Study." International Journal of Environmental Research and Public Health 18, no. 14: 7266.
The main expected result of a vaccine against viruses is the ability to produce neutralizing antibodies. Currently, several vaccines against SARS-CoV-2 are being applied to prevent mortal complications, being Pfizer-BioNTech (BNT162b2) one of the first to be authorized in the USA and Mexico (11 December 2020). This study evaluated the efficacy of this vaccine on antibody production with neutralizing capacity and its side effects in healthcare workers with and without prior SARS-CoV-2 infection and in a group of unvaccinated individuals with prior COVID-19. The main findings are the production of 100% neutralizing antibodies in both groups after the second dose, well-tolerated adverse effects, the possible presence of immunosenescence, and finally, we support that a single dose of this vaccine in individuals with prior COVID-19 would be sufficient to achieve an immunization comparable to people without prior COVID-19 with a complete vaccination program (2 doses).
José Morales-Núñez; José Muñoz-Valle; Carlos Meza-López; Lin-Fa Wang; Andrea Machado Sulbarán; Paola Torres-Hernández; Martín Bedolla-Barajas; Brenda De la O-Gómez; Paulina Balcázar-Félix; Jorge Hernández-Bello. Neutralizing Antibodies Titers and Side Effects in Response to BNT162b2 Vaccine in Healthcare Workers with and without Prior SARS-CoV-2 Infection. Vaccines 2021, 9, 742 .
AMA StyleJosé Morales-Núñez, José Muñoz-Valle, Carlos Meza-López, Lin-Fa Wang, Andrea Machado Sulbarán, Paola Torres-Hernández, Martín Bedolla-Barajas, Brenda De la O-Gómez, Paulina Balcázar-Félix, Jorge Hernández-Bello. Neutralizing Antibodies Titers and Side Effects in Response to BNT162b2 Vaccine in Healthcare Workers with and without Prior SARS-CoV-2 Infection. Vaccines. 2021; 9 (7):742.
Chicago/Turabian StyleJosé Morales-Núñez; José Muñoz-Valle; Carlos Meza-López; Lin-Fa Wang; Andrea Machado Sulbarán; Paola Torres-Hernández; Martín Bedolla-Barajas; Brenda De la O-Gómez; Paulina Balcázar-Félix; Jorge Hernández-Bello. 2021. "Neutralizing Antibodies Titers and Side Effects in Response to BNT162b2 Vaccine in Healthcare Workers with and without Prior SARS-CoV-2 Infection." Vaccines 9, no. 7: 742.
Systemic lupus erythematosus (SLE) is a chronic inflammatory disease with various clinical features. Autoreactive B cells play a role in disease pathogenesis, through the production of multiple autoantibodies, which form immune complexes and induce the inflammatory response and tissue damage associated with SLE. Recently, tetraspanins, and in particular, TSPAN32, have been recognized to play a central role in immunity, as they are involved in various biological processes, such as the antigen presentation and the activation of lymphocytes. Evidence suggests that tetraspanins could represent in the future a target for therapeutic purposes in patients suffering from autoimmune/immunoinflammatory disorders. In the present study, by performing in silico analyses of high-throughput data, we evaluated the expression levels of TSPAN32 in B cell activation and investigated its modulation in circulating B cells from SLE patients. Our data show that B cell activation is associated with a significant downregulation of TSPAN32. Additionally, significantly lower levels of TSPAN32 were observed in circulating plasmablasts from SLE patients as compared to healthy donor plasmablasts. In addition, type I interferons (IFNs)-related genes were enriched among the genes negatively correlated to TSPAN32, in SLE plasmablasts. Accordingly, IFN-α is able to induce a dose-dependent downregulation of TSPAN32 in B cells. Overall, the data here presented suggest the potential use of TSPAN32 as a diagnostic marker and therapeutic target for the evaluation and management of humoral immune responses in chronic diseases, such as SLE.
Paolo Fagone; Katia Mangano; Roberto Di Marco; Zyanya Reyes-Castillo; José Muñoz-Valle; Ferdinando Nicoletti. Altered Expression of TSPAN32 during B Cell Activation and Systemic Lupus Erythematosus. Genes 2021, 12, 931 .
AMA StylePaolo Fagone, Katia Mangano, Roberto Di Marco, Zyanya Reyes-Castillo, José Muñoz-Valle, Ferdinando Nicoletti. Altered Expression of TSPAN32 during B Cell Activation and Systemic Lupus Erythematosus. Genes. 2021; 12 (6):931.
Chicago/Turabian StylePaolo Fagone; Katia Mangano; Roberto Di Marco; Zyanya Reyes-Castillo; José Muñoz-Valle; Ferdinando Nicoletti. 2021. "Altered Expression of TSPAN32 during B Cell Activation and Systemic Lupus Erythematosus." Genes 12, no. 6: 931.
Background: The immunomodulatory effects of vitamin D are known to be beneficial in viral infections; it is also known that its deficiency is associated with a prognosis more critical of Coronavirus Disease 2019. This study aimed to determine baseline vitamin D serum concentrations and the effects of its supplementation in asymptomatic or mildly symptomatic Coronavirus Disease 2019 outpatients. Methods: 42 outpatients were included, 22 of which received a supplement of 10,000 IU of vitamin D3 for 14 days; the remaining 20 outpatients were designated as a control group. Serum levels of transferrin, ferritin, vitamin D, and D-dimer were measured at baseline in both groups. After 14 days, serum levels of total vitamin D were determined in the supplemented group. Results: At baseline, only 19% of infected outpatients had vitamin D levels corresponding to sufficiency. All outpatients with vitamin D insufficiency had at least one symptom associated with the disease, while only 75% of patients with symptoms presented sufficiency. On the seventh and fourteenth day of follow-up, the supplemented group presented fewer symptoms with respect to those non-supplemented. A vitamin D3 dose of 10,000 IU/daily for 14 days was sufficient to raise vitamin D serum concentrations. Conclusions: Immunomodulatory effects of vitamin D appear to be linked to the development of symptoms in positive outpatients. Vitamin D supplementation could have significant benefits in the Western Mexican population.
Gabriela Sánchez-Zuno; Guillermo González-Estevez; Mónica Matuz-Flores; Gabriela Macedo-Ojeda; Jorge Hernández-Bello; Jesús Mora-Mora; Edsaúl Pérez-Guerrero; Mariel García-Chagollán; Natali Vega-Magaña; Francisco Turrubiates-Hernández; Andrea Machado-Sulbaran; José Muñoz-Valle. Vitamin D Levels in COVID-19 Outpatients from Western Mexico: Clinical Correlation and Effect of Its Supplementation. Journal of Clinical Medicine 2021, 10, 2378 .
AMA StyleGabriela Sánchez-Zuno, Guillermo González-Estevez, Mónica Matuz-Flores, Gabriela Macedo-Ojeda, Jorge Hernández-Bello, Jesús Mora-Mora, Edsaúl Pérez-Guerrero, Mariel García-Chagollán, Natali Vega-Magaña, Francisco Turrubiates-Hernández, Andrea Machado-Sulbaran, José Muñoz-Valle. Vitamin D Levels in COVID-19 Outpatients from Western Mexico: Clinical Correlation and Effect of Its Supplementation. Journal of Clinical Medicine. 2021; 10 (11):2378.
Chicago/Turabian StyleGabriela Sánchez-Zuno; Guillermo González-Estevez; Mónica Matuz-Flores; Gabriela Macedo-Ojeda; Jorge Hernández-Bello; Jesús Mora-Mora; Edsaúl Pérez-Guerrero; Mariel García-Chagollán; Natali Vega-Magaña; Francisco Turrubiates-Hernández; Andrea Machado-Sulbaran; José Muñoz-Valle. 2021. "Vitamin D Levels in COVID-19 Outpatients from Western Mexico: Clinical Correlation and Effect of Its Supplementation." Journal of Clinical Medicine 10, no. 11: 2378.
Cardiovascular diseases (CVD) remain a serious public health problem and are the primary cause of death worldwide. High-density lipoprotein cholesterol (HDL-C) has been identified as one of the most important molecules in the prevention of CVD due to its multiple anti-inflammatories, anti-atherogenic, and antioxidant properties. Currently, it has been observed that maintaining healthy levels of HDL-C does not seem to be sufficient if the functionality of this particle is not adequate. Modifications in the structure and composition of HDL-C lead to a pro-inflammatory, pro-oxidant, and dysfunctional version of the molecule. Various assays have evaluated some HDL-C functions on risk populations, but they were not the main objective in some of these. Functional foods and dietary compounds such as extra virgin olive oil, nuts, whole grains, legumes, fresh fish, quercetin, curcumin, ginger, resveratrol, and other polyphenols could increase HDL functionality by improving the cholesterol efflux capacity (CEC), paraoxonase 1 (PON1), and cholesteryl ester transfer protein (CETP) activity. Nevertheless, additional rigorous research basic and applied is required in order to better understand the association between diet and HDL functionality. This will enable the development of nutritional precision management guidelines for healthy HDL to reduce cardiovascular risk in adults. The aim of the study was to increase the understanding of dietary compounds (functional foods and bioactive components) on the functionality of HDL.
Karla Luna-Castillo; Sophia Lin; José Muñoz-Valle; Barbara Vizmanos; Andres López-Quintero; Fabiola Márquez-Sandoval. Functional Food and Bioactive Compounds on the Modulation of the Functionality of HDL-C: A Narrative Review. Nutrients 2021, 13, 1165 .
AMA StyleKarla Luna-Castillo, Sophia Lin, José Muñoz-Valle, Barbara Vizmanos, Andres López-Quintero, Fabiola Márquez-Sandoval. Functional Food and Bioactive Compounds on the Modulation of the Functionality of HDL-C: A Narrative Review. Nutrients. 2021; 13 (4):1165.
Chicago/Turabian StyleKarla Luna-Castillo; Sophia Lin; José Muñoz-Valle; Barbara Vizmanos; Andres López-Quintero; Fabiola Márquez-Sandoval. 2021. "Functional Food and Bioactive Compounds on the Modulation of the Functionality of HDL-C: A Narrative Review." Nutrients 13, no. 4: 1165.
Background SARS‐CoV‐2 has become a global pandemic due to its capacity for rapid transmission. In this context, an early and rapid diagnosis of infected patients that do not require expensive equipment or highly trained personnel is crucial in order to reduce the contagious rate. The aim of this study was to evaluate a chromatographic immunoassay's performance for the rapid diagnosis of SARS‐CoV‐antigen. Methods A cross‐sectional study included 369 adults from Western México with diagnosis or suspicion of SARS‐CoV‐2 infection. Two samples were collected; a naso‐oropharyngeal was used for a molecular determination of SARS‐CoV‐2 RNA. The molecular analysis was carried out using DeCoV19 Kit Triplex (Genes2life S.A.P.I.) based on the CDC diagnostic panel for N1, N2, and N3 regions. The second sample was retrieved from a nasopharyngeal rub and used for the rapid diagnosis of SARS‐CoV‐2 antigen employing the commercial STANDARD™ Q COVID‐19 Ag Test (SD BIOSENSOR). Results Overall, in 28.2% of the patients was detected the SARS‐CoV‐2 RNA, and 21.4% were positive for antigen detection. The rapid antigen test showed a sensitivity and specificity of 75.9% and 100%, respectively, with a positive predictive and negative values of 100% and 91%. Symptoms as anosmia presented a high OR for the positive diagnosis for both test, reverse transcription‐polymerase chain reaction (RT‐PCR), and the rapid antigen test of 8.86 (CI = 4.91–16) and 6.09 (CI = 3.42–10.85), respectively. Conclusion SD BIOSENSOR is a useful assay, but some caveats must be considered before the general implementation.
Marcela Peña‐Rodríguez; Oliver Viera‐Segura; Mariel García‐Chagollán; José Sergio Zepeda‐Nuño; José Francisco Muñoz‐Valle; Jesús Mora‐Mora; Gabriela Espinoza‐De León; Gustavo Bustillo‐Armendáriz; Fernanda García‐Cedillo; Natali Vega‐Magaña. Performance evaluation of a lateral flow assay for nasopharyngeal antigen detection for SARS‐CoV‐2 diagnosis. Journal of Clinical Laboratory Analysis 2021, 35, e23745 .
AMA StyleMarcela Peña‐Rodríguez, Oliver Viera‐Segura, Mariel García‐Chagollán, José Sergio Zepeda‐Nuño, José Francisco Muñoz‐Valle, Jesús Mora‐Mora, Gabriela Espinoza‐De León, Gustavo Bustillo‐Armendáriz, Fernanda García‐Cedillo, Natali Vega‐Magaña. Performance evaluation of a lateral flow assay for nasopharyngeal antigen detection for SARS‐CoV‐2 diagnosis. Journal of Clinical Laboratory Analysis. 2021; 35 (5):e23745.
Chicago/Turabian StyleMarcela Peña‐Rodríguez; Oliver Viera‐Segura; Mariel García‐Chagollán; José Sergio Zepeda‐Nuño; José Francisco Muñoz‐Valle; Jesús Mora‐Mora; Gabriela Espinoza‐De León; Gustavo Bustillo‐Armendáriz; Fernanda García‐Cedillo; Natali Vega‐Magaña. 2021. "Performance evaluation of a lateral flow assay for nasopharyngeal antigen detection for SARS‐CoV‐2 diagnosis." Journal of Clinical Laboratory Analysis 35, no. 5: e23745.
Background Recurrent respiratory papillomatosis (RRP) is a respiratory tract disease that affects children and adults and is characterized by the recurrent proliferation of multiple papillomas. The etiologic agent is the human papillomavirus, mainly genotypes 6 and 11. Furthermore, polymorphisms in TAP1 appear to influence the selection of antigenic peptides and the transport process to the rough endoplasmic reticulum, for their subsequent presentation to T lymphocytes, an essential process against viral diseases and tumor processes. Previous studies have shown that individuals with those polymorphisms are susceptible to immune, infectious, and tumor‐related diseases. The present study aimed to determine the association between the TAP1 rs1057141 (c.1177A>G) and rs1135216 (c.2090A>G) single nucleotide polymorphisms (SNPs) and RRP. Methods A case–control study was carried out on a group of 70 individuals (35 controls and 35 patients). RRP diagnosis, HPV genotyping, and viral load were determined through histology and PCR. SNPs rs1057141 and rs1135216 were identified through allelic discrimination, using real‐time PCR. The haplotypic analyses were performed using the Arlequin 3.5 program. Results HPV‐6 and HPV‐11 were the genotypes found in the samples. In the polymorphism analysis, rs1057141 showed no significant differences (p = 0.049, CI = 0.994–7.331). In contrast, a significant difference was found in rs1135216 (p = 0.039, OR = 2.4) in the allelic analysis, as well as in the dominant (p = 0.027, OR = 3.06), codominant (p = 0.033, OR = 3.06), and additive model (p = 0.043, OR = 2.505) in subjects with the G allele. Conclusion The G allele in rs1135216 was associated with a genetic risk of susceptibility for RRP in a population in Western Mexico.
Jaime Palomares‐Marin; Luis Humberto Govea‐Camacho; Vania Araujo‐Caballero; Gerardo Cazarez‐Navarro; Sergio Yair Rodriguez‐Preciado; Enrique Ortiz‐Hernandez; Erika Martinez‐Lopez; Jose Francisco Muñoz‐Valle; Ivan Isidro Hernandez‐Cañaveral. Association between the TAP1 gene polymorphisms and recurrent respiratory papillomatosis in patients from Western Mexico: A pilot study. Journal of Clinical Laboratory Analysis 2021, 35, e23712 .
AMA StyleJaime Palomares‐Marin, Luis Humberto Govea‐Camacho, Vania Araujo‐Caballero, Gerardo Cazarez‐Navarro, Sergio Yair Rodriguez‐Preciado, Enrique Ortiz‐Hernandez, Erika Martinez‐Lopez, Jose Francisco Muñoz‐Valle, Ivan Isidro Hernandez‐Cañaveral. Association between the TAP1 gene polymorphisms and recurrent respiratory papillomatosis in patients from Western Mexico: A pilot study. Journal of Clinical Laboratory Analysis. 2021; 35 (4):e23712.
Chicago/Turabian StyleJaime Palomares‐Marin; Luis Humberto Govea‐Camacho; Vania Araujo‐Caballero; Gerardo Cazarez‐Navarro; Sergio Yair Rodriguez‐Preciado; Enrique Ortiz‐Hernandez; Erika Martinez‐Lopez; Jose Francisco Muñoz‐Valle; Ivan Isidro Hernandez‐Cañaveral. 2021. "Association between the TAP1 gene polymorphisms and recurrent respiratory papillomatosis in patients from Western Mexico: A pilot study." Journal of Clinical Laboratory Analysis 35, no. 4: e23712.
Psoriasis is a chronic, autoimmune skin disease. In psoriasis, PON1 activity is diminished and peroxidation biomarkers are elevated. The most studied PON1 polymorphisms are rs662 (A > G) and rs854560 (A > T), which have been associated with the antioxidant activity of PON1, risk of cardiovascular diseases and psoriasis development. The aim of this study, was to determine the association of rs662 (A > G) and rs854560 (A > T) PON1 polymorphisms with psoriasis susceptibility in Western Mexico population. In this case-control study, we included 104 psoriasis patients and 124 control subjects. The genotyping of polymorphisms rs662 (A > G) and rs854560 (A > T) of PON1 was carried out by PCR-RFLPs. The lipid profiles were quantified by enzymatic colorimetric method, and PON1 activity was determined by spectrophotometry. The lipid profile levels, except HDL-C and atherogenic index, were higher in patients vs. controls. Patients presented lower paraoxonase and arylesterase activity. The G allele of rs662 (A > G) is associated with risk for psoriasis, while the T allele of rs854560 (A > T) is associated with low susceptibility to psoriasis. The AG haplotype was more frequent within the patient group (p < 0.05). The AA and AG genotypes of rs662 (A > G) and TT and AA genotypes of rs854560 (A > T) are associated with lower PONase and ARE activity in patients vs. controls. Patients with the G allele of rs662 (G > A) and T alleles of rs854560 (A > T) show significant differences in the lipid levels in comparison to controls. These results suggest that carriers of G allele of rs662 (A > G) present a greater susceptibility to psoriasis.
A. A. Hernández-Collazo; Oscar Pérez-Méndez; Victoria López-Olmos; V. Delgado-Rizo; J. F. Muñoz-Valle; Erika Martínez-López; D. G. Villanueva-Quintero; Carolina Domínguez-Díaz; Mary Fafutis-Morris; Anabell Alvarado-Navarro. Association between rs662 (A > G) and rs854560 (A > T) polymorphisms in PON1 gene and the susceptibility for psoriasis in mestizo population of Western Mexico. Molecular Biology Reports 2020, 48, 183 -194.
AMA StyleA. A. Hernández-Collazo, Oscar Pérez-Méndez, Victoria López-Olmos, V. Delgado-Rizo, J. F. Muñoz-Valle, Erika Martínez-López, D. G. Villanueva-Quintero, Carolina Domínguez-Díaz, Mary Fafutis-Morris, Anabell Alvarado-Navarro. Association between rs662 (A > G) and rs854560 (A > T) polymorphisms in PON1 gene and the susceptibility for psoriasis in mestizo population of Western Mexico. Molecular Biology Reports. 2020; 48 (1):183-194.
Chicago/Turabian StyleA. A. Hernández-Collazo; Oscar Pérez-Méndez; Victoria López-Olmos; V. Delgado-Rizo; J. F. Muñoz-Valle; Erika Martínez-López; D. G. Villanueva-Quintero; Carolina Domínguez-Díaz; Mary Fafutis-Morris; Anabell Alvarado-Navarro. 2020. "Association between rs662 (A > G) and rs854560 (A > T) polymorphisms in PON1 gene and the susceptibility for psoriasis in mestizo population of Western Mexico." Molecular Biology Reports 48, no. 1: 183-194.
Background Metabolic syndrome (MetS) prevalence in rheumatoid arthritis (RA) patients is known to vary considerably across the world. This study aimed to determine the prevalence of MetS in RA patients from western Mexico and to analyze the interrelation of the MetS components with the clinical variables of RA. Methods This case‐control study included 216 RA patients and 260 control subjects (CS). MetS prevalence was determined according to the NCEP/ATP III and the Latin American Consensus of the Latin American Diabetes Association (ALAD) criteria. Results MetS was observed in 30.6% RA patients and 33.3% of controls (p > 0.05) according to NCEP/ATP III and 28.7% in RA patients and 31.1% for controls using ALAD criteria. Total cholesterol, LDL‐C, and Castelli's I‐II indexes were lower in RA (p < 0.001) than in CS. The RA patients with MetS had more swollen joints than those without MetS (p = 0.018). In RA patients with MetS, DAS‐28 score correlated with smoking index (rho = 0.4601, p = 0.0004) and VLDL‐C (rho = 0.3108, p = 0.0056); similarly, rheumatoid factor (RF) correlated with age (rho = 0.2031, p = 0.0027), smoking index (rho = 0.3404, p < 0.0001), triglycerides (rho = 0.1958, p = 0.0039), and VLDL‐C (rho = 0.1761, p = 0.0162). Conclusions The MetS prevalence in RA patients from western Mexico is not higher than controls; however, in RA patients with MetS, some inflammatory markers are associated with MetS components; thus, the control of MetS in RA could be beneficial to regulate disease activity.
Mariel García‐Chagollán; Susana Elizabeth Hernández‐Martínez; Alma Elizabeth Rojas‐Romero; José Francisco Muñoz‐Valle; Ramón Sigala‐Arellano; Sergio Cerpa‐Cruz; José Javier Morales‐Núñez; José Alvaro Lomelí‐Nieto; Gabriela Macedo Ojeda; Jorge Hernández‐Bello. Metabolic syndrome in rheumatoid arthritis patients: Relationship among its clinical components. Journal of Clinical Laboratory Analysis 2020, 35, e23666 .
AMA StyleMariel García‐Chagollán, Susana Elizabeth Hernández‐Martínez, Alma Elizabeth Rojas‐Romero, José Francisco Muñoz‐Valle, Ramón Sigala‐Arellano, Sergio Cerpa‐Cruz, José Javier Morales‐Núñez, José Alvaro Lomelí‐Nieto, Gabriela Macedo Ojeda, Jorge Hernández‐Bello. Metabolic syndrome in rheumatoid arthritis patients: Relationship among its clinical components. Journal of Clinical Laboratory Analysis. 2020; 35 (3):e23666.
Chicago/Turabian StyleMariel García‐Chagollán; Susana Elizabeth Hernández‐Martínez; Alma Elizabeth Rojas‐Romero; José Francisco Muñoz‐Valle; Ramón Sigala‐Arellano; Sergio Cerpa‐Cruz; José Javier Morales‐Núñez; José Alvaro Lomelí‐Nieto; Gabriela Macedo Ojeda; Jorge Hernández‐Bello. 2020. "Metabolic syndrome in rheumatoid arthritis patients: Relationship among its clinical components." Journal of Clinical Laboratory Analysis 35, no. 3: e23666.
Background: Several studies have shown that patients with cancer have antibodies in serum that react with cellular autoantigens, known as Tumor-Associated Antigens (TAA). The present work aimed to determine whether a mini-array comprising four recombinant TAA increases the detection of specific serum antibodies for the diagnosis of early-stage breast cancer. Methods: The mini-array included Alpha 1-AntiTrypsin (A1AT), TriosePhosphate Isomerase 1 (TPI1), Peptidyl-Prolyl cis-trans Isomerase A (PPIA), and PeroxiReDoXin 2 (PRDX2) full-length recombinant proteins. The proteins were produced after gene cloning, expression, and purification, and were verified by Western blot assays. Then, Dot-Blot was performed to find antibodies against the four TAA in 12 sera from women with early-stage breast cancer (stage II) and 12 sera from healthy women. Results: Antibody detection against individual TAA in early-stage breast cancer sera ranged from 58.3% to 83.3%. However, evaluation of the four TAA showed that there was a positive antibody reaction reaching a sensitivity of 100% and a specificity of 85% in early-stage breast cancer, suggesting that this mini-array must be evaluated as a clinical diagnostic tool for early-stage breast cancer in a larger sample size. Conclusion: Our results suggest that TAA mini-arrays may provide a promising and powerful method for improving the detection of breast cancer in Mexican women.
Alma Oaxaca-Camacho; Oscar Ochoa-Mojica; Adriana Aguilar-Lemarroy; Luis Jave-Suárez; José Muñoz-Valle; Eduardo Padilla-Camberos; Juan Núñez-Hernández; Sara Herrera-Rodríguez; Moisés Martínez-Velázquez; Ahtziri Carranza-Aranda; José Cruz-Ramos; Abel Gutiérrez-Ortega; Rodolfo Hernández-Gutiérrez. Serum Analysis of Women with Early-Stage Breast Cancer Using a Mini-Array of Tumor-Associated Antigens. Biosensors 2020, 10, 149 .
AMA StyleAlma Oaxaca-Camacho, Oscar Ochoa-Mojica, Adriana Aguilar-Lemarroy, Luis Jave-Suárez, José Muñoz-Valle, Eduardo Padilla-Camberos, Juan Núñez-Hernández, Sara Herrera-Rodríguez, Moisés Martínez-Velázquez, Ahtziri Carranza-Aranda, José Cruz-Ramos, Abel Gutiérrez-Ortega, Rodolfo Hernández-Gutiérrez. Serum Analysis of Women with Early-Stage Breast Cancer Using a Mini-Array of Tumor-Associated Antigens. Biosensors. 2020; 10 (10):149.
Chicago/Turabian StyleAlma Oaxaca-Camacho; Oscar Ochoa-Mojica; Adriana Aguilar-Lemarroy; Luis Jave-Suárez; José Muñoz-Valle; Eduardo Padilla-Camberos; Juan Núñez-Hernández; Sara Herrera-Rodríguez; Moisés Martínez-Velázquez; Ahtziri Carranza-Aranda; José Cruz-Ramos; Abel Gutiérrez-Ortega; Rodolfo Hernández-Gutiérrez. 2020. "Serum Analysis of Women with Early-Stage Breast Cancer Using a Mini-Array of Tumor-Associated Antigens." Biosensors 10, no. 10: 149.
Background Primary Sjögren's syndrome (pSS) is an autoimmune disease characterized by a lymphocytic infiltrate in salivary glands driving to epithelial damage. The pSS patients present heterogenic clinical and serological characteristics. This heterogenicity could be due to the cytokine microenvironment. Cytokine levels have been analyzed and reported individually, showing controversial results; for that reason, we considered essential to evaluate a cluster of cytokines and relate them with antibody levels and clinical characteristics to find pSS subgroups. Methods Ninety‐nine pSS patients, diagnosed by the 2016 ACR/EULAR classification criteria, and 76 control subjects (CS) were included. Cytokine quantification was performed by Multiplex assay. Principal component analysis (PCA) was realized, and the K‐mean test was used to identify clusters/groups. Groups were analyzed by the Kruskal‐Wallis test and the Bonferroni test. Results Higher IFN‐γ, IL‐17F, IL‐21, IL‐23, IL‐4, and IL‐31 levels were observed in pSS patients in comparison with control subjects. PCA analysis showed three groups. The severe group was characterized by higher cytokine concentrations as well as an increase in clinical parameters such as antibody levels, damage index score, and others. The moderate group presented intermediate severity; meanwhile, the mild group presented the lowest severity. Conclusion Cluster analysis revealed three groups that were different in cytokine levels and clinical parameters in which the mild group was defined by lower severity, the moderate group with intermediate severity, and the severe group with higher severity. This analysis could help subclassify the primary Sjögren syndrome patients for a better understanding of the clinical phenotype that impacts the treatment approach.
Erika Fabiola López‐Villalobos; José Francisco Muñoz‐Valle; Claudia Azucena Palafox‐Sánchez; Samuel García‐Arellano; Diana Emilia Martínez‐Fernández; Gerardo Orozco‐Barocio; José Antonio García‐Espinoza; Edith Oregon‐Romero. Cytokine profiles and clinical characteristics in primary Sjögren´s syndrome patient groups. Journal of Clinical Laboratory Analysis 2020, 35, e23629 .
AMA StyleErika Fabiola López‐Villalobos, José Francisco Muñoz‐Valle, Claudia Azucena Palafox‐Sánchez, Samuel García‐Arellano, Diana Emilia Martínez‐Fernández, Gerardo Orozco‐Barocio, José Antonio García‐Espinoza, Edith Oregon‐Romero. Cytokine profiles and clinical characteristics in primary Sjögren´s syndrome patient groups. Journal of Clinical Laboratory Analysis. 2020; 35 (2):e23629.
Chicago/Turabian StyleErika Fabiola López‐Villalobos; José Francisco Muñoz‐Valle; Claudia Azucena Palafox‐Sánchez; Samuel García‐Arellano; Diana Emilia Martínez‐Fernández; Gerardo Orozco‐Barocio; José Antonio García‐Espinoza; Edith Oregon‐Romero. 2020. "Cytokine profiles and clinical characteristics in primary Sjögren´s syndrome patient groups." Journal of Clinical Laboratory Analysis 35, no. 2: e23629.
Rheumatoid arthritis (RA) is an autoimmune and inflammatory disease that can cause joint damage. Among the environmental risk factors, diet plays an important role because it can aggravate or attenuate inflammation. Selenium (Se) is considered an essential trace element since it is a structural component of antioxidant enzymes; however, its concentration can be affected by diet, drugs and genetic polymorphisms. Studies have reported that RA patients have a deficient diet in some food groups that is associated with parameters of disease activity. Furthermore, it has been shown that there is an alteration in serum Se levels in this population. Although some clinical trials have been conducted in the past to analyze the effect of Se supplementation in RA, no significant results were obtained. Contrastingly, experimental studies that have evaluated the effect of novel Se nanoparticles in RA-induced models have shown promising results on the restoration of antioxidant enzyme levels. In particular, glutathione peroxidase (GPx) is an important selenoprotein that could have a modulating effect on inflammation in RA. Considering that RA patients present an inflammatory and oxidative state, the aim of this review is to give an overview of the current knowledge about the relevance of Se status in RA.
Francisco Javier Turrubiates-Hernández; Yolanda Fabiola Márquez-Sandoval; Guillermo González-Estevez; Zyanya Reyes-Castillo; José Francisco Muñoz-Valle. The Relevance of Selenium Status in Rheumatoid Arthritis. Nutrients 2020, 12, 3007 .
AMA StyleFrancisco Javier Turrubiates-Hernández, Yolanda Fabiola Márquez-Sandoval, Guillermo González-Estevez, Zyanya Reyes-Castillo, José Francisco Muñoz-Valle. The Relevance of Selenium Status in Rheumatoid Arthritis. Nutrients. 2020; 12 (10):3007.
Chicago/Turabian StyleFrancisco Javier Turrubiates-Hernández; Yolanda Fabiola Márquez-Sandoval; Guillermo González-Estevez; Zyanya Reyes-Castillo; José Francisco Muñoz-Valle. 2020. "The Relevance of Selenium Status in Rheumatoid Arthritis." Nutrients 12, no. 10: 3007.
The inflammatory process implicates homeostasis disruption and increased production of inflammatory mediators. Myeloid differentiation primary response 88 (MyD88) is an essential protein recruited after lipopolysaccharide (LPS) and interleukin (IL)-1β stimulation, a process that converges in nuclear factor kappa B (NF-κB) activation, as well as a transcription of several genes of both pro- and anti-inflammatory cytokines. The inhibition of MyD88 has shown efficacy by decrease inflammatory response, and has demonstrated potential application as a therapeutic target in chronic diseases. In this study, we investigate the effect of MyD88 dimerisation inhibitor ST2825 on cytokine production from rhIL-1β and LPS-stimulated peripheral blood mononuclear cells (PBMC) from healthy blood donors (HBD). ST2825 significantly downregulates the production of IFN-γ, IL-6, IL-12, IL-2, IL-15, IL-7, VEGF, IL-1Ra, IL-4, IL-5, IL-13 and IL-9 (p < 0.05) in LPS-stimulated PBMC. Moreover, ST2825 had a relatively low impact on IL-1β signalling pathway inhibition, showing that only a few specific cytokines, such as IFN-γ and IL-1Ra, are inhibited in rhIL-1β-stimulated PBMC (p < 0.01). In conclusion, MyD88 dimerisation inhibitor ST2825 showed high efficacy by inhibiting pro- and anti-inflammatory cytokine production in LPS-stimulated PBMC. Moreover, although rhIL-1β induced a sustained cytokine production (p < 0.05), ST2825 did not show a significant effect in the secretion of neither pro- nor anti-inflammatory cytokines in rhIL-1β-stimulated PBMC.
Sergio Ramírez-Pérez; Luis Alexis Hernández-Palma; Edith Oregon-Romero; Brian Uriel Anaya-Macías; Samuel García-Arellano; Guillermo González-Estevez; José Francisco Muñoz-Valle. Downregulation of Inflammatory Cytokine Release from IL-1β and LPS-Stimulated PBMC Orchestrated by ST2825, a MyD88 Dimerisation Inhibitor. Molecules 2020, 25, 4322 .
AMA StyleSergio Ramírez-Pérez, Luis Alexis Hernández-Palma, Edith Oregon-Romero, Brian Uriel Anaya-Macías, Samuel García-Arellano, Guillermo González-Estevez, José Francisco Muñoz-Valle. Downregulation of Inflammatory Cytokine Release from IL-1β and LPS-Stimulated PBMC Orchestrated by ST2825, a MyD88 Dimerisation Inhibitor. Molecules. 2020; 25 (18):4322.
Chicago/Turabian StyleSergio Ramírez-Pérez; Luis Alexis Hernández-Palma; Edith Oregon-Romero; Brian Uriel Anaya-Macías; Samuel García-Arellano; Guillermo González-Estevez; José Francisco Muñoz-Valle. 2020. "Downregulation of Inflammatory Cytokine Release from IL-1β and LPS-Stimulated PBMC Orchestrated by ST2825, a MyD88 Dimerisation Inhibitor." Molecules 25, no. 18: 4322.
Background The macrophage migration inhibiting factor (MIF) is a protein that promotes the activation of immune cells and the production of other proinflammatory cytokines such as TNF‐α, IL‐1β, and IFN‐γ, which have proposed to play an essential role in the pathogenesis of vitiligo. The study aimed to assess the association between MIF polymorphisms (−794 CATT5‐8 and −173 G>C), MIF in situ expression, and MIF serum concentrations with susceptibility and disease activity in patients with non‐segmental vitiligo (NSV) from western Mexico. Methods The study included 111 patients with NSV and 201 control subjects. Genotyping was performed by conventional PCR (−794 CATT5‐8) and PCR‐RFLP (−173 G>C) methods. MIF mRNA expression was quantified by real‐time PCR and MIF serum concentrations were determined by ELISA kit. Histopathological samples were analyzed by automated immunohistochemistry. Results The MIF polymorphisms were associated with NSV susceptibility. Serum concentrations of MIF were higher in patients with active NSV and correlated negatively with the years of evolution. The depigmented skin from patients with active vitiligo showed a high expression of MIF. Conclusion MIF polymorphisms increase the risk of NSV in the western Mexican population. The serum concentrations of MIF and in situ expression are associated with active NSV.
Alejandra Garcia‐Orozco; Itzel Alejandra Martinez‐Magaña; Annie Riera‐Leal; José Francisco Muñoz‐Valle; Marco Alonso Martinez‐Guzman; Ricardo Quiñones‐Venegas; Gabriela Athziri Sánchez‐Zuno; Mary Fafutis‐Morris. Macrophage inhibitory factor (MIF) gene polymorphisms are associated with disease susceptibility and with circulating MIF levels in active non‐segmental vitiligo in patients from western Mexico. Molecular Genetics & Genomic Medicine 2020, 8, 1 .
AMA StyleAlejandra Garcia‐Orozco, Itzel Alejandra Martinez‐Magaña, Annie Riera‐Leal, José Francisco Muñoz‐Valle, Marco Alonso Martinez‐Guzman, Ricardo Quiñones‐Venegas, Gabriela Athziri Sánchez‐Zuno, Mary Fafutis‐Morris. Macrophage inhibitory factor (MIF) gene polymorphisms are associated with disease susceptibility and with circulating MIF levels in active non‐segmental vitiligo in patients from western Mexico. Molecular Genetics & Genomic Medicine. 2020; 8 (10):1.
Chicago/Turabian StyleAlejandra Garcia‐Orozco; Itzel Alejandra Martinez‐Magaña; Annie Riera‐Leal; José Francisco Muñoz‐Valle; Marco Alonso Martinez‐Guzman; Ricardo Quiñones‐Venegas; Gabriela Athziri Sánchez‐Zuno; Mary Fafutis‐Morris. 2020. "Macrophage inhibitory factor (MIF) gene polymorphisms are associated with disease susceptibility and with circulating MIF levels in active non‐segmental vitiligo in patients from western Mexico." Molecular Genetics & Genomic Medicine 8, no. 10: 1.
After weeks under lockdown, metropolitan areas fighting the spread of COVID-19 aim to balance public health goals with social and economic standards for well-being. Mathematical models of disease transmission seeking to evaluate mitigation strategies must assess the possible impacts of social distancing, economic lockdowns and other measures. However, obscure relations between model parameters and real-world phenomena complicate such analyses. Here, we use a high-resolution metapopulation model of Guadalajara (GDL, Western Mexico) to represent daily mobility patterns driven by economic activities and their relation to epidemic growth. Given the prominence of essential activities in the city’s economy, we find that strategies aiming to mitigate the risk of out-of-home interactions are insufficient to stop the catastrophic spread of COVID-19. Using baseline reproduction numbers R0 = [2.5, 3.0] in the absence of interventions, our simulations suggest that household transmission alone can make Rt ∼ 1, and is estimated to drive 70 ±15% of current epidemic growth. This sets an upper bound for the impact of mobility-based interventions, which are unlikely to lower Rt below 1.3 and must be complemented with aggressive campaigns for early case detection and isolation. As laboratory testing and health services become insufficient to meet demand in GDL and most other cities, we propose that cities facilitate guidelines and equipment to help people curb spreading within their own homes. Postponing these actions will increase their economic cost and decrease their potential returns.Author summaryPublic health strategies to mitigate the spread of COVID-19 in metropolitan areas have focused on preventing transmission in schools, work sites and other public spaces. Here, we use a demographically- and spatially-explicit model of Guadalajara (GDL, Western Mexico) to represent economic lockdowns and their impact on disease spread. Our findings suggest that viral exposure within households accounts for 70±15% of the epidemic’s current growth rate. This highlights the importance of early case detection and isolation as necessary measures to prevent the spread of COVID-19 between strangers and close contacts alike.
Noel Gutiérrez Brizuela; Humberto Gutiérrez Pulido; Kimberlyn Roosa; Néstor García Chan; Jorge Hernández-Bello; José Francisco Muñoz-Valle; Gabriela Macedo-Ojeda; Guillermo González-Estevez; Javier Alonso López-Chávez; Ricardo Villanueva-Lomelí; Gerardo Chowell Puente. Prevention of household transmission crucial to stop the catastrophic spread of COVID-19 in cities. 2020, 1 .
AMA StyleNoel Gutiérrez Brizuela, Humberto Gutiérrez Pulido, Kimberlyn Roosa, Néstor García Chan, Jorge Hernández-Bello, José Francisco Muñoz-Valle, Gabriela Macedo-Ojeda, Guillermo González-Estevez, Javier Alonso López-Chávez, Ricardo Villanueva-Lomelí, Gerardo Chowell Puente. Prevention of household transmission crucial to stop the catastrophic spread of COVID-19 in cities. . 2020; ():1.
Chicago/Turabian StyleNoel Gutiérrez Brizuela; Humberto Gutiérrez Pulido; Kimberlyn Roosa; Néstor García Chan; Jorge Hernández-Bello; José Francisco Muñoz-Valle; Gabriela Macedo-Ojeda; Guillermo González-Estevez; Javier Alonso López-Chávez; Ricardo Villanueva-Lomelí; Gerardo Chowell Puente. 2020. "Prevention of household transmission crucial to stop the catastrophic spread of COVID-19 in cities." , no. : 1.
Functional variants -173 G > C (rs755622) and -794CATT5-8 (rs5844572) MIF gene have been associated with the risk in several types of cancer, as well as with the increase of soluble levels of MIF and TNFα. However, in previous studies contradictory and uncertain results have been presented on the implication of MIF polymorphisms with the association in cancer, specifically in breast cancer (BC). We investigated whether the variants are associated with the susceptibility to develop BC and the soluble levels of MIF and TNFα in women with BC from western Mexico. A total of 152 women with BC and 182 control subjects (CS) were enrolled in this study. The determination of genotypes -173 G > C and -794 CATT5-8 MIF polymorphisms was performed by PCR-RFLP and PCR, respectively. In addition, the soluble levels of MIF and TNFα in both studied groups were quantified by ELISA and MILLIPLEX assay, respectively. The most frequent allele found in BC was the G (74.3%) and 6 (54%) in the variants -173G > C and -794 CATT5-8 , respectively, without significant differences in both groups. Nevertheless, the women with BC carriers -173*C and -794CATT7 have higher levels of MIF in comparison with CS. An increase of MIF (BC: 11.1 ng/mL vs CS: 5.2 ng/mL, P < .001) and TNFα (BC: 24.9 ng/mL vs CS: 9.9 pg/mL, P < .001) was found. The functional variants of MIF are not genetic susceptibility markers for BC. Nevertheless, the alleles -173*C and -794CATT7 are associated with the increase of MIF circulating in women with BC.
Guadalupe Avalos Navarro; Alicia Del Toro‐Arreola; Adrian Daneri-Navarro; Antonio Quintero‐Ramos; Luis Alberto Bautista‐Herrera; Antonio Topete; Brian Uriel Anaya Macias; David Israel Javalera Castro; Andrés De Jesús Morán‐Mendoza; Antonio Oceguera‐Villanueva; Antonio Topete‐Camacho; José Francisco Muñoz‐Valle. Association of the genetic variants (‐794 CATT5‐8 and ‐173 G > C) of macrophage migration inhibitory factor (MIF) with higher soluble levels of MIF and TNFα in women with breast cancer. Journal of Clinical Laboratory Analysis 2020, 34, e23209 .
AMA StyleGuadalupe Avalos Navarro, Alicia Del Toro‐Arreola, Adrian Daneri-Navarro, Antonio Quintero‐Ramos, Luis Alberto Bautista‐Herrera, Antonio Topete, Brian Uriel Anaya Macias, David Israel Javalera Castro, Andrés De Jesús Morán‐Mendoza, Antonio Oceguera‐Villanueva, Antonio Topete‐Camacho, José Francisco Muñoz‐Valle. Association of the genetic variants (‐794 CATT5‐8 and ‐173 G > C) of macrophage migration inhibitory factor (MIF) with higher soluble levels of MIF and TNFα in women with breast cancer. Journal of Clinical Laboratory Analysis. 2020; 34 (5):e23209.
Chicago/Turabian StyleGuadalupe Avalos Navarro; Alicia Del Toro‐Arreola; Adrian Daneri-Navarro; Antonio Quintero‐Ramos; Luis Alberto Bautista‐Herrera; Antonio Topete; Brian Uriel Anaya Macias; David Israel Javalera Castro; Andrés De Jesús Morán‐Mendoza; Antonio Oceguera‐Villanueva; Antonio Topete‐Camacho; José Francisco Muñoz‐Valle. 2020. "Association of the genetic variants (‐794 CATT5‐8 and ‐173 G > C) of macrophage migration inhibitory factor (MIF) with higher soluble levels of MIF and TNFα in women with breast cancer." Journal of Clinical Laboratory Analysis 34, no. 5: e23209.
T-cell activation pathways have been proposed as trigger mechanisms in the pathogenesis of rheumatoid arthritis (RA). CD28 and CTLA-4 play major roles in regulating the stimulatory and inhibitory co-signals in T cells. To analyze the association between soluble and surface expression of CD28 and CTLA-4 with the clinical parameters of RA patients. A total of 35 RA patients classified as early RA (n = 14), chronic RA (n = 14), and untreated RA (n = 7), as well as 7 age- and sex-matched control subjects (CS) were included. Surface expression of CD28 and CTLA-4 on T cells was evaluated by flow cytometry. Soluble levels of CD28 (sCD28), CTLA-4 (sCTLA-4), and anti-CCP antibodies were measured by ELISA. A significant lower percentage of CD8 + T cells positive to CD28 (CS = 64.9% vs RA = 42.7%, P = .04), and diminished surface expression of CD28 (CS: MFI = 122.9 vs RA: MFI = 33.1, P = .006), were found in chronic RA patients compared to CS. Higher sCD28 were observed in early RA patients compared with chronic RA patients (P < .05). sCTLA-4 was found increased in untreated RA patients compared to early RA patients (P < .05). sCD28 concentration correlated with anti-CCP levels (rho = -0.12; P = .032). The soluble and surface expressions of CTLA-4 were not associated with RA clinical parameters. In RA, the percentage of CD8 + CD28+ T cells decreases and expresses fewer membrane CD28 than CS. sCD28 levels are lower in chronic RA and are associated negatively with anti-CCP levels. sCTLA 4 levels are lower in early RA patients than in untreated RA patients.
Mariel García-Chagollán; Iris Yolanda Ledezma-Lozano; Jorge Hernández-Bello; Pedro Ernesto Sánchez-Hernández; Sergio Ramón Gutiérrez-Ureña; José Francisco Muñoz-Valle. Expression patterns of CD28 and CTLA‐4 in early, chronic, and untreated rheumatoid arthritis. Journal of Clinical Laboratory Analysis 2020, 34, e23188 .
AMA StyleMariel García-Chagollán, Iris Yolanda Ledezma-Lozano, Jorge Hernández-Bello, Pedro Ernesto Sánchez-Hernández, Sergio Ramón Gutiérrez-Ureña, José Francisco Muñoz-Valle. Expression patterns of CD28 and CTLA‐4 in early, chronic, and untreated rheumatoid arthritis. Journal of Clinical Laboratory Analysis. 2020; 34 (5):e23188.
Chicago/Turabian StyleMariel García-Chagollán; Iris Yolanda Ledezma-Lozano; Jorge Hernández-Bello; Pedro Ernesto Sánchez-Hernández; Sergio Ramón Gutiérrez-Ureña; José Francisco Muñoz-Valle. 2020. "Expression patterns of CD28 and CTLA‐4 in early, chronic, and untreated rheumatoid arthritis." Journal of Clinical Laboratory Analysis 34, no. 5: e23188.
The goals of our study were to determine the possible association of interleukin (IL)-31 with Th17 cytokine profile in serum and to quantify retinoic acid-related orphan receptor C ( RORC) mRNA expression in psoriatic arthritis (PsA) patients. This cross-sectional study was conducted in 50 patients with PsA and 30 control subjects (CS) matched by age and gender. The cytokine serum levels were quantified by magnetic bead–based assay using the Bio-Plex MAGPIX system, and RORC mRNA expression was determined by quantitative polymerase chain reaction (qPCR). As a result, significant differences in IL-31 were observed between study groups (77.23 pg/mL in PsA vs 64.4 pg/mL in CS, P < 0.001) and Th17 cytokine profile serum levels (IL-17A: 6.36 pg/mL in PsA vs 2.97 pg/mL in CS, P = 0.02; IL-17F: 44.15 pg/mL in PsA vs 23.36 pg/mL in PsA, P = 0.01; IL-17E: 3.03 pg/mL in PsA vs 0.82 pg/mL in CS, P < 0.001; IL-21: 36.45 pg/mL in PsA vs 12.44 pg/mL in CS, P = 0.02); however, significant differences were not observed for IL-23 (31.2 pg/mL in PsA vs 53.26 pg/mL in CS, P = 0.58). Furthermore, positive correlations between IL-31 and Th17 cytokine profile serum levels were found (IL-17A: rs = 0.64, P < 0.001; IL-17F: rs = 0.73, P < 0.001; IL-17E: rs = 0.70, P < 0.001; IL-21: rs = 0.54, P = 0.002; IL-23: rs = 0.5, P < 0.01). Regarding RORC gene expression, the PsA group showed an increase of 6.85-fold compared to the CS group. We did not find any association between the serum levels of cytokines and RORC gene expression. In conclusion, in PsA, there are increased serum levels of IL-31, IL-17A, IL-17F, IL-17E, and IL-21, but not IL-23. Moreover, there was a positive correlation of IL-31 with the Th17 cytokine profile and a high RORC gene expression. Altogether, these findings suggest a proinflammatory contribution of IL-31 in close association with the Th17 cytokine profile in PsA.
L A Bautista-Herrera; U De La Cruz-Mosso; I V Román-Fernández; I Parra-Rojas; J G Soñanez-Organis; J Hernández-Bello; R A Morales-Zambrano; Delfina Guadalupe Villanueva; J F Muñoz-Valle. A potential inflammatory role of IL-31 in psoriatic arthritis: A correlation with Th17 cytokine profile. International Journal of Immunopathology and Pharmacology 2020, 34, 1 .
AMA StyleL A Bautista-Herrera, U De La Cruz-Mosso, I V Román-Fernández, I Parra-Rojas, J G Soñanez-Organis, J Hernández-Bello, R A Morales-Zambrano, Delfina Guadalupe Villanueva, J F Muñoz-Valle. A potential inflammatory role of IL-31 in psoriatic arthritis: A correlation with Th17 cytokine profile. International Journal of Immunopathology and Pharmacology. 2020; 34 ():1.
Chicago/Turabian StyleL A Bautista-Herrera; U De La Cruz-Mosso; I V Román-Fernández; I Parra-Rojas; J G Soñanez-Organis; J Hernández-Bello; R A Morales-Zambrano; Delfina Guadalupe Villanueva; J F Muñoz-Valle. 2020. "A potential inflammatory role of IL-31 in psoriatic arthritis: A correlation with Th17 cytokine profile." International Journal of Immunopathology and Pharmacology 34, no. : 1.