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Nikolaos Remmas
Department of Environmental Engineering, Laboratory of Wastewater Management and Treatment Technologies, Democritus University of Thrace, Vas. Sofias 12, 67132 Xanthi, Greece

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Journal article
Published: 19 January 2021 in Life
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The evaluation of effluent wastewater quality mainly relies on the assessment of conventional bacterial indicators, such as fecal coliforms and enterococci; however, little is known about opportunistic pathogens, which can resist chlorination and may be transmitted in aquatic environments. In contrast to conventional microbiological methods, high-throughput molecular techniques can provide an accurate evaluation of effluent quality, although a limited number of studies have been performed in this direction. In this work, high-throughput amplicon sequencing was employed to assess the effectiveness of chlorination as a disinfection method for secondary effluents. Common inhabitants of the intestinal tract, such as Bacteroides, Arcobacter and Clostridium, and activated sludge denitrifiers capable of forming biofilms, such as Acidovorax, Pseudomonas and Thauera, were identified in the chlorinated effluent. Chloroflexi with dechlorination capability and the bacteria involved in enhanced biological phosphorus removal, i.e., Candidatus Accumulibacter and Candidatus Competibacter, were also found to resist chlorination. No detection of Escherichia indicates the lack of fecal coliform contamination. Mycobacterium spp. were absent in the chlorinated effluent, whereas toxin-producing cyanobacteria of the genera Anabaena and Microcystis were identified in low abundances. Chlorination significantly affected the filamentous bacteria Nocardioides and Gordonia, whereas Zoogloea proliferated in the disinfected effluent. Moreover, perchlorate/chlorate- and organochlorine-reducing bacteria resisted chlorination.

ACS Style

Ioanna Zerva; Nikolaos Remmas; Ifigeneia Kagalou; Paraschos Melidis; Marina Ariantsi; Georgios Sylaios; Spyridon Ntougias. Effect of Chlorination on Microbiological Quality of Effluent of a Full-Scale Wastewater Treatment Plant. Life 2021, 11, 68 .

AMA Style

Ioanna Zerva, Nikolaos Remmas, Ifigeneia Kagalou, Paraschos Melidis, Marina Ariantsi, Georgios Sylaios, Spyridon Ntougias. Effect of Chlorination on Microbiological Quality of Effluent of a Full-Scale Wastewater Treatment Plant. Life. 2021; 11 (1):68.

Chicago/Turabian Style

Ioanna Zerva; Nikolaos Remmas; Ifigeneia Kagalou; Paraschos Melidis; Marina Ariantsi; Georgios Sylaios; Spyridon Ntougias. 2021. "Effect of Chlorination on Microbiological Quality of Effluent of a Full-Scale Wastewater Treatment Plant." Life 11, no. 1: 68.

Journal article
Published: 22 August 2019 in Processes
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Despite that diclofenac has been embodied to the European watch list of priority substances of concern, studies on diclofenac biodegradation are limited and the diversity of diclofenac-degrading microbiota remains unknown. In this work, an immobilized cell biofilter was constructed and operated to evaluate its effectiveness to depurate high strength diclofenac wastewater and to identify the diclofenac-degrading community accommodated in activated sludge by employing high-throughput sequencing techniques. After a two-month adaptation period, biofilter removal efficiencies reached values as high as 97.63 ± 0.62%, whereas utilization of diclofenac in the immobilized cell biofilter led to a drastic pH decrease. Based on Illumina sequencing, the major bacterial taxa identified in the immobilized cell biofilter were members of the species Granulicella pectinivorans and Rhodanobacter terrae, followed by members of the species Castellaniella denitrificans, Parvibaculum lavamentivorans, Bordetella petrii, Bryocella elongata and Rhodopseudomonas palustris. The ability of such taxa to utilize a wide range of carbon sources and to effectively adapt under acidic conditions seemed to be the main parameters, which favored their prevalence in the immobilized cell biofilter. In addition, Wickerhamiella was the predominant fungal taxon in the immobilized cell biofilter, which appears to be actively involved in diclofenac degradation in activated sludge systems.

ACS Style

Efstathia Navrozidou; Nikolaos Remmas; Paraschos Melidis; Dimitrios G. Karpouzas; George Tsiamis; Spyridon Ntougias. Biodegradation Potential and Diversity of Diclofenac-degrading Microbiota in an Immobilized Cell Biofilter. Processes 2019, 7, 554 .

AMA Style

Efstathia Navrozidou, Nikolaos Remmas, Paraschos Melidis, Dimitrios G. Karpouzas, George Tsiamis, Spyridon Ntougias. Biodegradation Potential and Diversity of Diclofenac-degrading Microbiota in an Immobilized Cell Biofilter. Processes. 2019; 7 (9):554.

Chicago/Turabian Style

Efstathia Navrozidou; Nikolaos Remmas; Paraschos Melidis; Dimitrios G. Karpouzas; George Tsiamis; Spyridon Ntougias. 2019. "Biodegradation Potential and Diversity of Diclofenac-degrading Microbiota in an Immobilized Cell Biofilter." Processes 7, no. 9: 554.

Journal article
Published: 02 March 2019 in Beverages
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Cellulases can be applied as macerating and peeling enzymes in the orange juice processing industry. In this work, indigenous cellulose-degrading microorganisms were isolated from orange juice processing waste through successive enrichment procedures using carboxymethyl cellulose (CMC) as the sole carbon source. A total of 24 microbial isolates were screened for their ability to grow in CMC liquid medium, resulting in the selection of seven isolates. The latter were further assessed by determining their endo-1,4-β-d-glucanase, exo-1,4-β-d-glucanase, and β-1,4-d-glucosidase activities, of which their respective activities were as high as 3.89, 10.67, and 10.69 U/mg protein. All cellulose-degraders selected belonged to the genus Paenibacillus, although to distinct operational taxonomic units related to P. xylanexedens, P. tundrae, and P. pabuli (operational taxonomic unit—OTU#1) and to P. wynnii, P. odorifer, and P. donghaensis (OTU#2) spectrum. Regarding the cellulase activities of the orange juice processing waste, endo-1,4-β-d-glucanase activity (4.00 ± 0.11 U/g) was exerted only extracellularly, whereas exo-1,4-β-d-glucanase (2.60 ± 0.19 U/g) and β-1,4-d-glucosidase (5.69 ± 0.23 U/g) activities were exhibited both extracellularly and intracellularly. In conclusion, orange juice processing waste can be considered as a valuable source for the isolation of cellulose-degrading microbiota with potential uses in beverage industry, solid state fermentation and energy production.

ACS Style

Ioanna Zerva; Nikolaos Remmas; Spyridon Ntougias. Biocatalyst Potential of Cellulose-Degrading Microorganisms Isolated from Orange Juice Processing Waste. Beverages 2019, 5, 21 .

AMA Style

Ioanna Zerva, Nikolaos Remmas, Spyridon Ntougias. Biocatalyst Potential of Cellulose-Degrading Microorganisms Isolated from Orange Juice Processing Waste. Beverages. 2019; 5 (1):21.

Chicago/Turabian Style

Ioanna Zerva; Nikolaos Remmas; Spyridon Ntougias. 2019. "Biocatalyst Potential of Cellulose-Degrading Microorganisms Isolated from Orange Juice Processing Waste." Beverages 5, no. 1: 21.

Journal article
Published: 05 February 2019 in Water
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The orange juice processing sector produces worldwide massive amounts of waste, which is characterized by high lignin, cellulose and hemicellulose content, and which exceeds 40% of the fruit’s dry weight (d.w.). In this work, the diversity and the biotechnological potential of xylan-degrading microbiota in orange juice processing waste were investigated through the implementation of an enrichment isolation strategy followed by enzyme assays for the determination of xylanolytic activities, and via next generation sequencing for microbial diversity identification. Intracellular rather than extracellular endo-1,4-β-xylanase activities were detected, indicating that peripheral cell-bound (surface) xylanases are involved in xylan hydrolysis by the examined microbial strains. Among the isolated microbial strains, bacterial isolates belonging to Pseudomonas psychrotolerans/P. oryzihabitans spectrum (99.9%/99.8% similarity, respectively) exhibited activities of 280 U/mg protein. In contrast, almost all microbial strains isolated exerted low extracellular 1,4-β-xylosidase activities (

ACS Style

Ioanna Zerva; Nikolaos Remmas; Spyridon Ntougias. Diversity and Biotechnological Potential of Xylan-Degrading Microorganisms from Orange Juice Processing Waste. Water 2019, 11, 274 .

AMA Style

Ioanna Zerva, Nikolaos Remmas, Spyridon Ntougias. Diversity and Biotechnological Potential of Xylan-Degrading Microorganisms from Orange Juice Processing Waste. Water. 2019; 11 (2):274.

Chicago/Turabian Style

Ioanna Zerva; Nikolaos Remmas; Spyridon Ntougias. 2019. "Diversity and Biotechnological Potential of Xylan-Degrading Microorganisms from Orange Juice Processing Waste." Water 11, no. 2: 274.