This page has only limited features, please log in for full access.
Mimosa pudica Linn is a well-known perennial herb and is traditionally used in ayurvedic medicine for the treatment of various illnesses. Despite its abundance in nature, the therapeutic potential of this invasive weed is deemed to be underappreciated in Malaysia. Previous studies have found an abundance of bioactive compounds associated with potent antioxidant properties in all parts of the plant. However, the optimum parameters required for the extraction of antioxidant compounds are still unknown. Therefore, the present study aimed to optimize the solvent extraction parameters of M. pudica using response surface methodology to enrich the accumulation of antioxidant compounds in the extracts. The effects of the optimized M. pudica extracts were then evaluated on the cell viability and glucose uptake ability in a 3T3-L1 adipocyte cell line. The highest total phenolic (91.98 mg of gallic acid equivalent per g of the dry extract) and total flavonoid content (606.31 mg of quercetin equivalent per g of the dry extract) were recorded when using 100% ethanol that was five-fold and three-fold higher, respectively, as compared to using 50% ethanol. The extract concentration required to achieve 50% of antioxidant activity (IC50 value) was 42.0 µg/mL using 100% ethanol as compared to 975.03 µg/mL using 50% ethanol. The results indicated that the use of 100% ethanol solvent had the greatest impact on the accumulation of antioxidant compounds in the extract (p< 0.05). Cell viability assay revealed that all extract concentration treatments recorded a viability level of above 50%. Glucose uptake assay using 2-NBDG analog showed that the cells treated with 50 µg/mL extract combined with insulin were five-fold higher than the control group. Given the high antioxidant and antidiabetic properties of this plant, M. pudica can be easily highlighted as a plant subject of interest, which warrants further investigation for nutraceutical prospects.
Nor Saffana Baharuddin; Muhamad Aidilfitri Mohamad Roslan; Mohsen Ahmed Mohammed Bawzer; Azzreena Mohamad Azzeme; Zuraida Ab Rahman; Mohd Ezuan Khayat; Nor Aini Abdul Rahman; Zulfazli M. Sobri. Response Surface Optimization of Extraction Conditions and In Vitro Antioxidant and Antidiabetic Evaluation of an Under-Valued Medicinal Weed, Mimosa pudica. Plants 2021, 10, 1692 .
AMA StyleNor Saffana Baharuddin, Muhamad Aidilfitri Mohamad Roslan, Mohsen Ahmed Mohammed Bawzer, Azzreena Mohamad Azzeme, Zuraida Ab Rahman, Mohd Ezuan Khayat, Nor Aini Abdul Rahman, Zulfazli M. Sobri. Response Surface Optimization of Extraction Conditions and In Vitro Antioxidant and Antidiabetic Evaluation of an Under-Valued Medicinal Weed, Mimosa pudica. Plants. 2021; 10 (8):1692.
Chicago/Turabian StyleNor Saffana Baharuddin; Muhamad Aidilfitri Mohamad Roslan; Mohsen Ahmed Mohammed Bawzer; Azzreena Mohamad Azzeme; Zuraida Ab Rahman; Mohd Ezuan Khayat; Nor Aini Abdul Rahman; Zulfazli M. Sobri. 2021. "Response Surface Optimization of Extraction Conditions and In Vitro Antioxidant and Antidiabetic Evaluation of an Under-Valued Medicinal Weed, Mimosa pudica." Plants 10, no. 8: 1692.
The production of alkaloids in the intact plant is usually low, and some of them are tissue-specific and growth stage–specific. In this study, the total alkaloid content (TAC) in Polyalthia bullata was determined using different extraction solvents, and the extracts were subjected to LC-MS analysis. The manipulation of alkaloid production in P. bullata callus was further carried out by growing the callus on two basal media (Murashige and Skoog (MS) and Woody Plant Medium (WPM)) containing auxins (2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthaleneacetic acid (NAA), 4-amino-3,5,6-trichloropicolinic acid (picloram), 3,6-dichloro-o-anisic acid (dicamba), indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA)), precursors (L-phenylalanine, L-tyrosine, and L-tryptophan), and elicitors (methyl jasmonate (MeJA), salicylic acid (SA), and chitosan). The methanolic extracts exhibited the highest alkaloid content in leaf, stem, and root tissues (4.61 ± 0.04 to 7.71 ± 0.04 mg/g DW), and nine alkaloids were detected in P. bullata. The callus grown on MS + 30 μM 2,4-D exhibited the highest alkaloid accumulation (31.07 ± 0.05 μg/mg DW) after 6 weeks of culture compared to other media: MS media containing auxins, MS media containing auxins and precursors, and MS media containing auxins and elicitors. Therefore, we conclude that the MS + 30 μM 2,4-D is potent in enhancing alkaloid production in P. bullata callus.
Munirah Adibah Kamarul Zaman; Azzreena Mohamad Azzeme; Illy Kamaliah Ramle; Nurfazlinyana Normanshah; Noor Azmi Shaharuddin; Syahida Ahmad; Siti Nor Akmar Abdullah. Prolonged incubation of callus on auxin herbicide 2,4-D displayed significant effect on alkaloid production in callus of the woody medicinal plant Polyalthia bullata. In Vitro Cellular & Developmental Biology - Animal 2021, 1 -11.
AMA StyleMunirah Adibah Kamarul Zaman, Azzreena Mohamad Azzeme, Illy Kamaliah Ramle, Nurfazlinyana Normanshah, Noor Azmi Shaharuddin, Syahida Ahmad, Siti Nor Akmar Abdullah. Prolonged incubation of callus on auxin herbicide 2,4-D displayed significant effect on alkaloid production in callus of the woody medicinal plant Polyalthia bullata. In Vitro Cellular & Developmental Biology - Animal. 2021; ():1-11.
Chicago/Turabian StyleMunirah Adibah Kamarul Zaman; Azzreena Mohamad Azzeme; Illy Kamaliah Ramle; Nurfazlinyana Normanshah; Noor Azmi Shaharuddin; Syahida Ahmad; Siti Nor Akmar Abdullah. 2021. "Prolonged incubation of callus on auxin herbicide 2,4-D displayed significant effect on alkaloid production in callus of the woody medicinal plant Polyalthia bullata." In Vitro Cellular & Developmental Biology - Animal , no. : 1-11.
Polyalthia bullata is an endangered medicinal plant species. Hence, establishment of P. bullata callus culture is hoped to assist in mass production of secondary metabolites. Leaf and midrib were explants for callus induction. Both of them were cultured on Murashige and Skoog (MS) and Woody Plant Medium (WPM) containing different types and concentrations of auxins (2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthaleneacetic acid (NAA), picloram, and dicamba). The callus produced was further multiplied on MS and WPM supplemented with different concentrations of 2,4-D, NAA, picloram, dicamba, indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA) media. The quantification of total phenolic content (TPC), total flavonoid content (TFC) and antioxidant capacity was further carried out on P. bullata callus, and the results were subjected to correlation analysis. Among the media, the WPM + 16.56 µM picloram (53.33 ± 22.06%) was the best for callus induction while MS + 30 µM dicamba was the best for callus multiplication. The TPC, TFC, and EC50 of DPPH scavenging activity were determined at 0.657 ± 0.07 mg GAE/g FW, 0.491 ± 0.03 mg QE/g, and 85.59 ± 6.09 µg/mL in P. bullata callus, respectively. The positive correlation between DPPH scavenging activity with TPC was determined at r = 0.869, and that of TFC was at r = 0.904. Hence, the P. bullata callus has an ability to accumulate antioxidants. It therefore can be a medium for secondary metabolites production.
Munirah Adibah Kamarul Zaman; Azzreena Mohamad Azzeme; Illy Kamaliah Ramle; Nurfazlinyana Normanshah; Siti Nurhafizah Ramli; Noor Azmi Shaharuddin; Syahida Ahmad; Siti Nor Akmar Abdullah. Induction, Multiplication, and Evaluation of Antioxidant Activity of Polyalthia bullata Callus, A Woody Medicinal Plant. Plants 2020, 9, 1772 .
AMA StyleMunirah Adibah Kamarul Zaman, Azzreena Mohamad Azzeme, Illy Kamaliah Ramle, Nurfazlinyana Normanshah, Siti Nurhafizah Ramli, Noor Azmi Shaharuddin, Syahida Ahmad, Siti Nor Akmar Abdullah. Induction, Multiplication, and Evaluation of Antioxidant Activity of Polyalthia bullata Callus, A Woody Medicinal Plant. Plants. 2020; 9 (12):1772.
Chicago/Turabian StyleMunirah Adibah Kamarul Zaman; Azzreena Mohamad Azzeme; Illy Kamaliah Ramle; Nurfazlinyana Normanshah; Siti Nurhafizah Ramli; Noor Azmi Shaharuddin; Syahida Ahmad; Siti Nor Akmar Abdullah. 2020. "Induction, Multiplication, and Evaluation of Antioxidant Activity of Polyalthia bullata Callus, A Woody Medicinal Plant." Plants 9, no. 12: 1772.
Azzreena Mohamad Azzeme. Influence of different extraction solvents on phytochemical content and antioxidant capacity extracted from pulp and flower of dessert and cooking bananas. 2020, 1 .
AMA StyleAzzreena Mohamad Azzeme. Influence of different extraction solvents on phytochemical content and antioxidant capacity extracted from pulp and flower of dessert and cooking bananas. . 2020; ():1.
Chicago/Turabian StyleAzzreena Mohamad Azzeme. 2020. "Influence of different extraction solvents on phytochemical content and antioxidant capacity extracted from pulp and flower of dessert and cooking bananas." , no. : 1.
Polyalthia bullata is a woody medicinal plant that contains antioxidant compounds. Finding a suitable solvent is important to obtain a high yield of antioxidants in the phenolic, flavonoid, and terpenoid families. In this study, from different solvent extracts, the leaf methanolic extract exhibited the highest total phenolic content (TPC), total flavonoid content (TFC), total terpenoid content (TTC), and total antioxidant activity. For woody parts of stem and roots, methanol was the best solvent for all phytochemicals except for phenolics, which accumulated in the roots and were extracted more efficiently using ethanol. However, the methanolic extracts from both tissues displayed the best antioxidant capacity. Gas chromatography-mass spectrometry (GC-MS) profiling data showed the presence of antioxidant compounds such as thymol, phytol, and neophytadiene in the leaf; trans-farnesol, n-hexadecanoic acid, and 9-Octadecenamide in the stem; and fatty acid (cis-vaccenic) and its methyl ester (11-Octadecanoic acid, methyl ester and [1,1’-bicyclopropyl]-2-octanoic acid, 2’-hexyl-methyl ester) in the roots. These findings reveal important compounds that are present in different plant parts of P. bullata.
Munirah Adibah Kamarul Zaman; Azzreena Mohamad Azzeme; Siti Nurhafizah Ramli; Noor Azmi Shaharuddin; Syahida Ahmad; Siti Nor Akmar Abdullah. Solvent extraction and its effect on phytochemical yield and antioxidant capacity of woody medicinal plant, Polyalthia bullata. BioResources 2020, 15, 1 .
AMA StyleMunirah Adibah Kamarul Zaman, Azzreena Mohamad Azzeme, Siti Nurhafizah Ramli, Noor Azmi Shaharuddin, Syahida Ahmad, Siti Nor Akmar Abdullah. Solvent extraction and its effect on phytochemical yield and antioxidant capacity of woody medicinal plant, Polyalthia bullata. BioResources. 2020; 15 (4):1.
Chicago/Turabian StyleMunirah Adibah Kamarul Zaman; Azzreena Mohamad Azzeme; Siti Nurhafizah Ramli; Noor Azmi Shaharuddin; Syahida Ahmad; Siti Nor Akmar Abdullah. 2020. "Solvent extraction and its effect on phytochemical yield and antioxidant capacity of woody medicinal plant, Polyalthia bullata." BioResources 15, no. 4: 1.
Azzreena Mohamad Azzeme; S.N.A. Abdullah; M.A. Aziz; P.E. Wahab. Overexpression of oil palm EgDREB1 in tomato decreased fruit sizeand produced parthenocarpic fruits. Biologia plantarum 2020, 64, 58 -67.
AMA StyleAzzreena Mohamad Azzeme, S.N.A. Abdullah, M.A. Aziz, P.E. Wahab. Overexpression of oil palm EgDREB1 in tomato decreased fruit sizeand produced parthenocarpic fruits. Biologia plantarum. 2020; 64 (1):58-67.
Chicago/Turabian StyleAzzreena Mohamad Azzeme; S.N.A. Abdullah; M.A. Aziz; P.E. Wahab. 2020. "Overexpression of oil palm EgDREB1 in tomato decreased fruit sizeand produced parthenocarpic fruits." Biologia plantarum 64, no. 1: 58-67.
Salinization process occurs when soil is contaminated with salt, which consequently influences plant growth and development leading to reduction in yield of many food crops. Responding to a higher salt concentration than the normal range can result in plant developing complex physiological traits and activation of stress-related genes and metabolic pathways. Many studies have been carried out by different research groups to understand adaptive mechanism in many plant species towards salinity stress. However, different methods of sodium chloride (NaCl) applications definitely give different responses and adaptive mechanisms towards the increase in salinity. Gradual increase in NaCl application causes the plant to have salt stress or osmotic stress, while single step and high concentration of NaCl may result in salt shock or osmotic shock. Osmotic shock can cause cell plasmolysis and leakage of osmolytes in plant. Also, the gene expression pattern is influenced by the type of methods used in increasing the salinity. Therefore, this chapter discusses the adaptive mechanism in plant responding to both types of salinity increment, which include the morphological changes of plant roots and aerial parts, involvement of signalling molecules in stress perception and regulatory networks and production of osmolyte and osmoprotective proteins.
Azzreena Mohamad Azzeme; Siti Nor Akmar Abdullah. Adaptive Mechanisms of Plants Against Salt Stress and Salt Shock. Salt Stress, Microbes, and Plant Interactions: Mechanisms and Molecular Approaches 2019, 27 -47.
AMA StyleAzzreena Mohamad Azzeme, Siti Nor Akmar Abdullah. Adaptive Mechanisms of Plants Against Salt Stress and Salt Shock. Salt Stress, Microbes, and Plant Interactions: Mechanisms and Molecular Approaches. 2019; ():27-47.
Chicago/Turabian StyleAzzreena Mohamad Azzeme; Siti Nor Akmar Abdullah. 2019. "Adaptive Mechanisms of Plants Against Salt Stress and Salt Shock." Salt Stress, Microbes, and Plant Interactions: Mechanisms and Molecular Approaches , no. : 27-47.
Munirah Adibah Kamarul Zaman; Azzreena Mohamad Azzeme. Plant toxins: alkaloids and their toxicities. GSC Biological and Pharmaceutical Sciences 2018, 6, 021 -029.
AMA StyleMunirah Adibah Kamarul Zaman, Azzreena Mohamad Azzeme. Plant toxins: alkaloids and their toxicities. GSC Biological and Pharmaceutical Sciences. 2018; 6 (2):021-029.
Chicago/Turabian StyleMunirah Adibah Kamarul Zaman; Azzreena Mohamad Azzeme. 2018. "Plant toxins: alkaloids and their toxicities." GSC Biological and Pharmaceutical Sciences 6, no. 2: 021-029.
Oil palm is the most productive oil crop which plays a key role in meeting current and future demand for vegetable oil. Palm oil has a multitude of edible and nonedible applications and it is an important contributor to the economy of a few countries in the Southeast Asian region such as Malaysia, Indonesia, and Thailand. Water, temperature, and nutritional stresses are identified as key factors suppressing oil palm productivity. Addressing issues in key biological processes such as fruit development and responses to abiotic stress is essential in oil palm genetic improvement efforts. Gene expression at the transcriptional level is mainly regulated by transcription factors (TFs) which mediate cellular signaling responses and coordinate expression of biosynthetic pathway genes. Several transcription factors of the APETALA2/ethylene response factor (AP2/ERF) family including from the AP2 and ERF subfamilies have been identified to be involved in fruit ripening while the dehydration responsive element-binding 1 (DREB1)/C-repeat-binding factor (CBF) subfamilies are associated with abiotic stress response in oil palm. Their expression profiles have been studied in oil palm tissues at different stages of development and in response to various phytohormones and abiotic factors as well as in transgenic tomato as a model system. Their DNA binding abilities to specific motifs in stress-responsive and fruit ripening associated genes have been characterized through yeast one-hybrid and electrophoretic mobility shift assay (EMSA). This chapter also looks at other transcription factor families that have been shown to regulate these important biological processes in oil palm. The potential applications of the transcription factors for crop improvement efforts are discussed.
Siti Nor Akmar Abdullah; Azzreena Mohamad Azzeme; Mortaza Ebrahimi; Engku Ahmad Khairi Engku Ariff; Farah Hanan Abu Hanifiah. Transcription Factors Associated with Abiotic Stress and Fruit Development in Oil Palm. Crop Improvement 2017, 71 -99.
AMA StyleSiti Nor Akmar Abdullah, Azzreena Mohamad Azzeme, Mortaza Ebrahimi, Engku Ahmad Khairi Engku Ariff, Farah Hanan Abu Hanifiah. Transcription Factors Associated with Abiotic Stress and Fruit Development in Oil Palm. Crop Improvement. 2017; ():71-99.
Chicago/Turabian StyleSiti Nor Akmar Abdullah; Azzreena Mohamad Azzeme; Mortaza Ebrahimi; Engku Ahmad Khairi Engku Ariff; Farah Hanan Abu Hanifiah. 2017. "Transcription Factors Associated with Abiotic Stress and Fruit Development in Oil Palm." Crop Improvement , no. : 71-99.
Dehydration-responsive element binding (DREB) transcription factor plays an important role in controlling the expression of abiotic stress responsive genes. An intronless oil palm EgDREB1 was isolated and confirmed to be a nuclear localized protein. Electrophoretic mobility shift and yeast one-hybrid assays validated its ability to interact with DRE/CRT motif. Its close evolutionary relation to the dicot NtDREB2 suggests a universal regulatory role. In order to determine its involvement in abiotic stress response, functional characterization was performed in oil palm seedlings subjected to different levels of drought severity and in EgDREB1 transgenic tomato seedlings treated by abiotic stresses. Its expression in roots and leaves was compared with several antioxidant genes using quantitative real-time PCR. Early accumulation of EgDREB1 in oil palm roots under mild drought suggests possible involvement in the initiation of signaling communication from root to shoot. Ectopic expression of EgDREB1 in T1 transgenic tomato seedlings enhanced expression of DRE/CRT and non-DRE/CRT containing genes, including tomato peroxidase (LePOD), ascorbate peroxidase (LeAPX), catalase (LeCAT), superoxide dismutase (LeSOD), glutathione reductase (LeGR), glutathione peroxidase (LeGP), heat shock protein 70 (LeHSP70), late embryogenesis abundant (LeLEA), metallothionine type 2 (LeMET2), delta 1-pyrroline-5- carboxylate synthetase (LePCS), ABA-aldehyde oxidase (LeAAO) and 9-cis- Epoxycarotenoid dioxygenase (LeECD) under PEG treatment and cold stress (4 °C). Altogether, these findings suggest that EgDREB1 is a functional regulator in enhancing tolerance to drought and cold stress.
Azzreena Mohamad Azzeme; Siti Nor Akmar Abdullah; Maheran Abd Aziz; Puteri Edaroyati Megat Wahab. Oil palm drought inducible DREB1 induced expression of DRE/CRT- and non-DRE/CRT-containing genes in lowland transgenic tomato under cold and PEG treatments. Plant Physiology and Biochemistry 2017, 112, 129 -151.
AMA StyleAzzreena Mohamad Azzeme, Siti Nor Akmar Abdullah, Maheran Abd Aziz, Puteri Edaroyati Megat Wahab. Oil palm drought inducible DREB1 induced expression of DRE/CRT- and non-DRE/CRT-containing genes in lowland transgenic tomato under cold and PEG treatments. Plant Physiology and Biochemistry. 2017; 112 ():129-151.
Chicago/Turabian StyleAzzreena Mohamad Azzeme; Siti Nor Akmar Abdullah; Maheran Abd Aziz; Puteri Edaroyati Megat Wahab. 2017. "Oil palm drought inducible DREB1 induced expression of DRE/CRT- and non-DRE/CRT-containing genes in lowland transgenic tomato under cold and PEG treatments." Plant Physiology and Biochemistry 112, no. : 129-151.
The seedling stage is a critical period for survival under drought stress. To identify biochemical and molecular drought response changes, oil palm seedlings were exposed to different levels of drought severity. Total chlorophyll, total soluble protein and total proline content were measured while expression of stress responsive genes was quantified using qPCR. The diminishing total chlorophyll (chl) content and the ratio of chla to chlb (chla:chlb) were observed to be significant. The significant reduction of chla was closely related to photosystem II deficiency. Based on the effects of drought on chlorophyll content, the samples can be categorised into mild (7 days of water withholding; DWW), moderate (14 DWW) and severe (21, 28 and 35 DWW). Sample at 21 DWW was used to represent the severe stage. Genes encoding ethylene responsive binding protein, late embryogenesis abundant (LEA), dehydrin (DHN), cold-induced, heat shock protein 70 and metallothionein type 2 were differentially up-regulated in the leaves, while in the roots only LEA and DHN were up-regulated. The proline content increased gradually in both vegetative tissues, while the total soluble protein content was affected by increasing drought severity. The activity of catalase was highest in the roots at the severe drought stage, while guaicol peroxidase activity was shown to be highest in the leaves under mild drought. These findings provide new insights into stress tolerance mechanisms of oil palm seedlings and can be used to develop stress tolerant oil palm through classical breeding and genetic engineering.
Azzreena Mohamad Azzeme; Siti Nor Akmar Abdullah; Maheran Abdul Aziz; Puteri Edaroyati Megat Wahab. Oil palm leaves and roots differ in physiological response, antioxidant enzyme activities and expression of stress-responsive genes upon exposure to drought stress. Acta Physiologiae Plantarum 2016, 38, 1 -12.
AMA StyleAzzreena Mohamad Azzeme, Siti Nor Akmar Abdullah, Maheran Abdul Aziz, Puteri Edaroyati Megat Wahab. Oil palm leaves and roots differ in physiological response, antioxidant enzyme activities and expression of stress-responsive genes upon exposure to drought stress. Acta Physiologiae Plantarum. 2016; 38 (2):1-12.
Chicago/Turabian StyleAzzreena Mohamad Azzeme; Siti Nor Akmar Abdullah; Maheran Abdul Aziz; Puteri Edaroyati Megat Wahab. 2016. "Oil palm leaves and roots differ in physiological response, antioxidant enzyme activities and expression of stress-responsive genes upon exposure to drought stress." Acta Physiologiae Plantarum 38, no. 2: 1-12.
Azzreena Mohamad Azzeme. Effects of over-expressing ethylene responsive transcription factor on expression of selected fruit ripening-related genes in oil palm (Elaeis guineensis Jacq.) mesocar. 2015, 1 .
AMA StyleAzzreena Mohamad Azzeme. Effects of over-expressing ethylene responsive transcription factor on expression of selected fruit ripening-related genes in oil palm (Elaeis guineensis Jacq.) mesocar. . 2015; ():1.
Chicago/Turabian StyleAzzreena Mohamad Azzeme. 2015. "Effects of over-expressing ethylene responsive transcription factor on expression of selected fruit ripening-related genes in oil palm (Elaeis guineensis Jacq.) mesocar." , no. : 1.