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Post-graduate in the field of Plant Biotechnology. Currently working on the project related to the plant secondary metabolite and tissue culture of herbs.
Polyalthia bullata is an endangered medicinal plant species. Hence, establishment of P. bullata callus culture is hoped to assist in mass production of secondary metabolites. Leaf and midrib were explants for callus induction. Both of them were cultured on Murashige and Skoog (MS) and Woody Plant Medium (WPM) containing different types and concentrations of auxins (2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthaleneacetic acid (NAA), picloram, and dicamba). The callus produced was further multiplied on MS and WPM supplemented with different concentrations of 2,4-D, NAA, picloram, dicamba, indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA) media. The quantification of total phenolic content (TPC), total flavonoid content (TFC) and antioxidant capacity was further carried out on P. bullata callus, and the results were subjected to correlation analysis. Among the media, the WPM + 16.56 µM picloram (53.33 ± 22.06%) was the best for callus induction while MS + 30 µM dicamba was the best for callus multiplication. The TPC, TFC, and EC50 of DPPH scavenging activity were determined at 0.657 ± 0.07 mg GAE/g FW, 0.491 ± 0.03 mg QE/g, and 85.59 ± 6.09 µg/mL in P. bullata callus, respectively. The positive correlation between DPPH scavenging activity with TPC was determined at r = 0.869, and that of TFC was at r = 0.904. Hence, the P. bullata callus has an ability to accumulate antioxidants. It therefore can be a medium for secondary metabolites production.
Munirah Adibah Kamarul Zaman; Azzreena Mohamad Azzeme; Illy Kamaliah Ramle; Nurfazlinyana Normanshah; Siti Nurhafizah Ramli; Noor Azmi Shaharuddin; Syahida Ahmad; Siti Nor Akmar Abdullah. Induction, Multiplication, and Evaluation of Antioxidant Activity of Polyalthia bullata Callus, A Woody Medicinal Plant. Plants 2020, 9, 1772 .
AMA StyleMunirah Adibah Kamarul Zaman, Azzreena Mohamad Azzeme, Illy Kamaliah Ramle, Nurfazlinyana Normanshah, Siti Nurhafizah Ramli, Noor Azmi Shaharuddin, Syahida Ahmad, Siti Nor Akmar Abdullah. Induction, Multiplication, and Evaluation of Antioxidant Activity of Polyalthia bullata Callus, A Woody Medicinal Plant. Plants. 2020; 9 (12):1772.
Chicago/Turabian StyleMunirah Adibah Kamarul Zaman; Azzreena Mohamad Azzeme; Illy Kamaliah Ramle; Nurfazlinyana Normanshah; Siti Nurhafizah Ramli; Noor Azmi Shaharuddin; Syahida Ahmad; Siti Nor Akmar Abdullah. 2020. "Induction, Multiplication, and Evaluation of Antioxidant Activity of Polyalthia bullata Callus, A Woody Medicinal Plant." Plants 9, no. 12: 1772.
Munirah Adibah Kamarul Zaman; Azzreena Mohamad Azzeme. Plant toxins: alkaloids and their toxicities. GSC Biological and Pharmaceutical Sciences 2018, 6, 021 -029.
AMA StyleMunirah Adibah Kamarul Zaman, Azzreena Mohamad Azzeme. Plant toxins: alkaloids and their toxicities. GSC Biological and Pharmaceutical Sciences. 2018; 6 (2):021-029.
Chicago/Turabian StyleMunirah Adibah Kamarul Zaman; Azzreena Mohamad Azzeme. 2018. "Plant toxins: alkaloids and their toxicities." GSC Biological and Pharmaceutical Sciences 6, no. 2: 021-029.