Wetlands cover a small portion of the world, but have disproportionate influence on global carbon (C) sequestration, carbon dioxide and methane emissions, and aquatic C fluxes. However, the underlying biogeochemical processes that affect wetland C pools and fluxes are complex and dynamic, making measurements of wetland C challenging. Over decades of research, many observational, experimental, and analytical approaches have been developed to understand and quantify pools and fluxes of wetland C. Sampling approaches range in their representation of wetland C from short to long timeframes and local to landscape spatial scales. This review summarizes common and cutting-edge methodological approaches for quantifying wetland C pools and fluxes. We first define each of the major C pools and fluxes and provide rationale for their importance to wetland C dynamics. For each approach, we clarify what component of wetland C is measured and its spatial and temporal representativeness and constraints. We describe practical considerations for each approach, such as where and when an approach is typically used, who can conduct the measurements (expertise, training requirements), and how approaches are conducted, including considerations on equipment complexity and costs. Finally, we review key covariates and ancillary measurements that enhance the interpretation of findings and facilitate model development. The protocols that we describe to measure soil, water, vegetation, and gases are also relevant for related disciplines such as ecology. Improved quality and consistency of data collection and reporting across studies will help reduce global uncertainties and develop management strategies to use wetlands as nature-based climate solutions.

The Prairie Pothole Region (PPR) contains numerous depressional wetlands known as potholes that provide habitats for waterfowl and other wetland-dependent species. Mapping these wetlands is essential for identifying viable waterfowl habitat and conservation planning scenarios, yet it is a challenging task due to the small size of the potholes, and the presence of emergent vegetation. This study develops an open-source process within the Google Earth Engine platform for mapping the spatial distribution of wetlands through the integration of Sentinel-1 C-band SAR (synthetic aperture radar) data with high-resolution (10-m) Sentinel-2 bands. We used two machine-learning algorithms (random forest (RF) and support vector machine (SVM)) to identify wetlands across the study area through supervised classification of the multisensor composite. We trained the algorithms with ground truth data provided through field studies and aerial photography. The accuracy was assessed by comparing the predicted and actual wetland and non-wetland classes using statistical coefficients (overall accuracy, Kappa, sensitivity, and specificity). For this purpose, we used four different out-of-sample test subsets, including the same year, next year, small vegetated, and small non-vegetated test sets to evaluate the methods on different spatial and temporal scales. The results were also compared to Landsat-derived JRC surface water products, and the Sentinel-2-derived normalized difference water index (NDWI). The wetlands derived from the RF model (overall accuracy 0.76 to 0.95) yielded favorable results, and outperformed the SVM, NDWI, and JRC products in all four testing subsets. To provide a further characterization of the potholes, the water bodies were stratified based on the presence of emergent vegetation using Sentinel-2-derived NDVI, and, after excluding permanent water bodies, using the JRC surface water product. The algorithm presented in the study is scalable and can be adopted for identifying wetlands in other regions of the world.

Elevation differences in salt marshes result in numerous ecological consequences as a result of variation in tidal flooding. We demonstrate here that elevation differences are also negatively correlated with soil temperature on the marsh platform, irrespective of tidal flooding. Field observations of soil temperature at 10‐cm depth in a Georgia marsh showed that elevation increases of 0.5 m corresponded to decreases in average soil temperature of 0.9–1.7°C during both winter and summer. Landsat 8 estimates of land surface temperatures across the marsh in dry (nonflooded) scenes also showed that temperature decreased with increasing elevation, which was consistent with soil observations. Similar satellite results were also found in a test marsh in Virginia. Biological reactions are temperature‐dependent, and these findings indicate that metabolic processes will vary over short distances. This is important for accurately estimating marsh metabolism and predicting how changes in temperature will affect future productivity.