Cassava production generates significant amounts of by-products such as leaves and tuber peel. Instead of considering them as waste, valorization aims to find sustainable ways to utilize them. However, the presence of cyanide and insoluble fibers poses a major obstacle to their conversion into valuable products. Therefore, the objective of this study is to investigate the changes in cyanide concentration and quality of cassava leaves after mechanical pressing and in tuber peel after treatment with an enzyme solution. Frozen leaves were screw-pressed into their fractions: juice, and press cake. The results show that the cyanide level in the press cake was reduced to 73.56% and was concentrated by 97.48% in the juice compared to the frozen leaves. However, the crude protein values of the frozen leaves, juice, and press cake did not differ significantly (p > 0.05), and were 27.09%, 25.47%, and 23.82%, respectively. In addition, the results for the peel revealed that pretreatment with Viscozyme® L, which assists in the mechanical peeling of cassava tubers, also contributed to a reduction in cyanide and insoluble fiber in the peel. Cyanide content was lowered by 53.89–58.94% in enzyme-treated peel from all three runs (ETP1-3) when compared to fresh peel (FP), while the reduction was only 8.63% in the control peel (CP) treated with hot water without enzyme solution. The insoluble fibers in cassava peel, such as neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), and crude fiber (CF), were also degraded more effectively after treatment with an enzyme solution than with hot water.
Mango has been described as a valuable source of nutrients and enzymes that are beneficial to human health. Drying at different temperatures not only affects the nutritional properties but can also contribute to the degradation of valuable enzymes in dried fruit. The novelty of this paper is to investigate the quality of hot air dried mango in terms of activity retention of the heat-sensitive enzymes (HSE). For this, HSE was first screened in fresh mango flesh of the variety Samar Bahisht (SB) Chaunsa. Later, the combined effect of different drying temperatures (40 °C, 50 °C, 60 °C, 70 °C, and 80 °C) and air velocities (1.0 ms−1 and 1.4 ms−1) on the activity retention of HSE in dried mango slices of the varieties Sindri, SB Chaunsa, and Tommy Atkins were investigated. The results showed that the drying temperature had a significant impact on the degradation of HSE, while at the same time some influence of the air velocity was also observed. Drying at 40 °C and an air velocity of 1.4 ms−1 retained more HSE compared to those samples dried at higher temperatures. The least retention of HSE was found in samples dried at 80 °C.
The growing market for dried fruits requires more attention to quality parameters. Mango and other tropical fruits are commonly dried at temperatures ranging from 40 °C to 80 °C. Convincing evidence suggests that the nutritional quality of dried fruits is best preserved when dried at low temperatures ≤50 °C, whereas increasing drying temperatures lead to the degradation of the most valuable nutrients inside the fruit. Currently, there is no system or direct measurement method that can assist in identifying the quality deterioration of dried fruits caused by excessive heat exposure during drying. From this perspective, the activity of the heat-sensitive enzyme ‘catalase’ was used for the first time to evaluate and compare mango slices dried at 40 °C, 60 °C and 80 °C. Various methods, including direct and indirect flotation tests and spectrophotometric measurements, were explored to measure the residual catalase activity in the dried samples. Results showed that the spectrophotometry and indirect flotation test produced the best results, revealing a significant difference (p < 0.05) in the catalase activity of mango slices dried at 40 °C, 60 °C and 80 °C, which the direct-dried mango flotation test failed to predict. Furthermore, this study demonstrates the potential applicability of catalase activity to indicate heat stress in dried mango slices processed at different temperatures.
Recently, fruit-drying industries are showing great interest in producing dry fruits that preserve a high enzyme content. Therefore, this study aimed to investigate the effect of hot-air convective drying on activity retention of amylase and invertase in dried mango of varieties Sindri, Samar Bahisht (SB) Chaunsa, and Tommy Atkins. Convection drying was conducted under over-flow mode at five temperatures (40, 50, 60, 70, and 80 °C), two air velocities (1.0 and 1.4 m s−1), and constant specific humidity of 10 g kg−1 dry air. The enzymatic degradation data were fitted to the first-order reaction kinetics model, in which the temperature dependence of the rate constant is modelled by the Arrhenius-type relationship. Results showed that the maximum amylase and invertase activity for dried mango of all three varieties was best preserved in samples dried at a temperature of 80 °C and an air velocity of 1.4 m s−1. In contrast, a lower drying temperature and an air velocity of 1.0 m s−1 contributed to a significant decrease (p < 0.05). Exploration of different temperatures and air velocities to save amylase and invertase in dried mango is useful from an industrial point of view, as mango can be a natural dietary source of digestive enzymes to improve digestion.