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Dr. Hossam Darrag
KING FAISAL UNIVERSITY

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0 Metabolomics
0 secondary metabolites
0 Biosynthesis
0 natural product
0 biopesticide

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Journal article
Published: 30 April 2021 in Insects
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The current study was designed to investigate the insecticide role of volatile constituents produced from cell suspensions of T. vulgaris and O. basilicum against R. ferrugineus. Constituents were extracted from cell suspension after 40 days. Growth kinetics were measured with an inoculation of Verticillium dahliae and identified by GC-MS. Total volatile phenolic constituents were measured. Insecticidal activity against R. ferrugineus (adult) and proteolytic enzyme activity in larvae were assessed. GC-MS showed that the T. vulgaris extract has higher amounts of thymol, p-cymene, γ-terpinene, β-caryophyllene, and linalool in comparison to the O. basilicum extract, which is rich in estragole, β-terpineol, (E)-β-ocimene, 1,8-cineole, germacrene D, and eugenol. The T. vulgaris extract showed an LC50 of 1032 µg/mL, followed by O. basilicum with an LC50 of 1246 µg/mL. The IC50 values against the total proteases were 110.8 and 119.4 µg/mL for T. vulgaris and O. basilicum, respectively. The IC50 for the trypsin-like serine proteinase assessment was 81.6 and 91 µg/mL for T. vulgaris and O. basilicum, respectively. Cysteine, chymotrypsin, and metalloproteinase assessment showed an IC50 above 5000 µg/mL for both extracts. The study is proposed as a potential approach to use T. vulgaris and O. basilicum extract as a bio-insecticide against R. ferrugineus using an accessible and efficient cell suspension technique.

ACS Style

Hossam Darrag; Mohammed Alhajhoj; Hany Khalil. Bio-Insecticide of Thymus vulgaris and Ocimum basilicum Extract from Cell Suspensions and Their Inhibitory Effect against Serine, Cysteine, and Metalloproteinases of the Red Palm Weevil (Rhynchophorus ferrugineus). Insects 2021, 12, 405 .

AMA Style

Hossam Darrag, Mohammed Alhajhoj, Hany Khalil. Bio-Insecticide of Thymus vulgaris and Ocimum basilicum Extract from Cell Suspensions and Their Inhibitory Effect against Serine, Cysteine, and Metalloproteinases of the Red Palm Weevil (Rhynchophorus ferrugineus). Insects. 2021; 12 (5):405.

Chicago/Turabian Style

Hossam Darrag; Mohammed Alhajhoj; Hany Khalil. 2021. "Bio-Insecticide of Thymus vulgaris and Ocimum basilicum Extract from Cell Suspensions and Their Inhibitory Effect against Serine, Cysteine, and Metalloproteinases of the Red Palm Weevil (Rhynchophorus ferrugineus)." Insects 12, no. 5: 405.

Journal article
Published: 09 April 2021 in Plants
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Date palm fruit (Phoenix dactylifera) is reputed to have numerous biological activities, including anticancer properties. To utilize the great fortune of this fruit, the current study aimed to maximize its pharmacological activity. Date palm extract (DPE) of Khalas cultivar was obtained in powder form and then was formulated into nanoemulsion (NE). The optimized DPE-NE was formulated along with its naked counterpart followed by studying their physical and chemical properties. A qualitative assessment of total serum protein associated with the surface of formulations was implemented. Studies for the in vitro release of DPE from developed NE before and after incubation with serum were investigated. Eventually, an MTT assay was conducted. Total phenolic and flavonoid contents were 22.89 ± 0.013 mg GAE/g of dry DPE and 9.90 ± 0.03 mg QE/g of dry DPE, respectively. Homogenous NE formulations were attained with appropriate particle size and viscosity that could be administered intravenously. The optimized PEGylated NE exhibited a proper particle size, PDI, and zeta potential. Total serum protein adsorbed on PEG-NE surface was significantly low. The release of the drug through in vitro study was effectively extended for 24 h. Ultimately; PEGylated NE of DPE attained significant inhibition for cancer cell viability with IC50 values of 18.6 ± 2.4 and 13.5 ± 1.8 µg/mL for MCF-7 and HepG2 cell lines, respectively. PEGylated NE of DPE of Khalas cultivar will open the gate for future adjuvants for cancer therapy.

ACS Style

Hany Khalil; Nashi Alqahtani; Hossam Darrag; Hairul-Islam Ibrahim; Promise Emeka; Lorina Badger-Emeka; Katsuyoshi Matsunami; Tamer Shehata; Heba Elsewedy. Date Palm Extract (Phoenix dactylifera) PEGylated Nanoemulsion: Development, Optimization and Cytotoxicity Evaluation. Plants 2021, 10, 735 .

AMA Style

Hany Khalil, Nashi Alqahtani, Hossam Darrag, Hairul-Islam Ibrahim, Promise Emeka, Lorina Badger-Emeka, Katsuyoshi Matsunami, Tamer Shehata, Heba Elsewedy. Date Palm Extract (Phoenix dactylifera) PEGylated Nanoemulsion: Development, Optimization and Cytotoxicity Evaluation. Plants. 2021; 10 (4):735.

Chicago/Turabian Style

Hany Khalil; Nashi Alqahtani; Hossam Darrag; Hairul-Islam Ibrahim; Promise Emeka; Lorina Badger-Emeka; Katsuyoshi Matsunami; Tamer Shehata; Heba Elsewedy. 2021. "Date Palm Extract (Phoenix dactylifera) PEGylated Nanoemulsion: Development, Optimization and Cytotoxicity Evaluation." Plants 10, no. 4: 735.

Journal article
Published: 20 November 2020 in Plants
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Trigonella stellata has used in folk medicine as palatable and nutraceutical herb. It also regulates hypocholesterolemia, hypoglycemia, and has showed anti-inflammatory activities as well as antioxidants efficacy. Osteoporosis is a one of bone metabolic disorders and is continuously increasing worldwide. In the present study, caffeic acid was isolated from Trigonella stellata and identified using 1 D- and 2 D-NMR spectroscopic data. Caffeic acid was investigated on osteoblast and osteoclast in vitro using mice bone marrow-derived mesenchymal cells. Caffeic acid played reciprocal proliferation between osteoblast and osteoclast cells and accelerated the bone mineralization. It was confirmed by cytotoxicity, alkaline phosphatase (ALP), alizarin red S (ARS), and Tartrate resistant acid phosphatase (TRAP) assay. Caffeic acid regulated the osteogenic marker and upregulated the osteopontin, osteocalcin, and bone morphogenic proteins (BMP). Quantitative real time PCR and Western blot were used to quantify the mRNA and protein markers. It also regulated the matrix metalloprotease-2 (MMP-2) and cathepsin-K proteolytic markers in osteoclast cells. In addition, caffeic acid inhibited bone resorption in osteoclast cells. On the other hand, it upregulate osteoblast differentiation through stimulation of extracellular calcium concentrations osteoblast differentiation, respectively. The results also were confirmed through in silico docking of caffeic acid against cathepsin-B and cathepsin-K markers. These findings revealed that caffeic acid has a potential role in bone-metabolic disorder through its multifaceted effects on osteoblast and osteoclast regulations and controls osteoporosis.

ACS Style

Hairul-Islam Mohamed Ibrahim; Hossam M. Darrag; Mohammed Refdan Alhajhoj; Hany Ezzat Khalil. Biomolecule from Trigonella stellata from Saudi Flora to Suppress Osteoporosis via Osteostromal Regulations. Plants 2020, 9, 1610 .

AMA Style

Hairul-Islam Mohamed Ibrahim, Hossam M. Darrag, Mohammed Refdan Alhajhoj, Hany Ezzat Khalil. Biomolecule from Trigonella stellata from Saudi Flora to Suppress Osteoporosis via Osteostromal Regulations. Plants. 2020; 9 (11):1610.

Chicago/Turabian Style

Hairul-Islam Mohamed Ibrahim; Hossam M. Darrag; Mohammed Refdan Alhajhoj; Hany Ezzat Khalil. 2020. "Biomolecule from Trigonella stellata from Saudi Flora to Suppress Osteoporosis via Osteostromal Regulations." Plants 9, no. 11: 1610.