This feeding trial was conducted to investigate the effects of dietary black seed (Nigella sativa) supplementation on the growth performance, muscles proximate composition, antioxidant and histo-biochemical parameters of rohu (Labeo rohita). Fingerlings (8.503 ± 0.009 g) were fed on 0.0%, 1% and 2.5% black seed supplemented diets for 28 days. Fish sampling was done on the 7th, 14th, 21st and 28th day of experiment. The results of the present study indicated that black seed supplementation significantly increased growth performance and muscles protein contents of rohu over un-supplemented ones. Lipid peroxidation levels significantly decreased in all the studied tissues (liver, gills, kidney and brain) of black seed fed rohu, whereas the antioxidant enzymes (catalase, glutathione-S-transferase, glutathione peroxidase and reduced glutathione) activities were increased in all the studied tissues of black seed supplemented rohu at each sampling day. The hepatic-nephric marker enzymes levels were decreased for black seed fed rohu. The present study showed that tested black seed levels are safe for rohu. Black seed is cheaply available in local markets of Pakistan; therefore, based on the results of the present study, it is suggested that black seed has potential to be used as natural growth promoter and antioxidant in the diet of rohu.

The present study was carried out to estimate the residual level of preservatives and active whitening agents from personal care products (PCPs) wastewater

River Chenab is an important wetland in Pakistan supporting diverse fauna and flora. Present study deals with the icthyodiversity of river Chenab at Trimmu Headwork and reports 43 species belonging to six orders, 13 families and 34 genera. On the basis of number and percentage contribution of families to their orders, order Siluriformes was found dominant represented with five families (38.5%) followed by Perciformes (30.7%). Fish species belonging to family Cyprinidae were found dominant (44.2%) in the present collection. Systomus sarana has shown highest value of relative abundance followed by Puntius sophore and Barilius modestus. Population of commercially important fish species has declined in this river due to the various anthropogenic pressures. The values for species diversity, richness and evenness from the study area were 3.56, 13.98 and 0.95 respectively. Conservation measures are strongly recommended for protecting this diverse fish fauna.