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Age-related macular degeneration (AMD) is one of the main causes of deterioration in vision in adults aged 55 and older. In spite of therapies, the progression of the disease is often observed without reverse vision quality. In the present study, we explored whether, in undifferentiated ARPE-19 retinal cells, a disruption of the VEGF receptors (VEGF-R)/caveolin-1 (Cav-1)/protein kinases pathway could be a target for counteracting VEGF secretion. We highlight that Resvega®, a combination of omega-3 fatty acids with an antioxidant, resveratrol, inhibits VEGF-A secretion in vitro by disrupting the dissociation of the VEGF-R2/Cav-1 complex into rafts and subsequently preventing MAPK activation. Moreover, DNA ChIP analysis reveals that this combination prevents the interaction between AP-1 and vegf-a and vegf-r2 gene promoters. By these pathways, Resvega could present a potential interest as nutritional complementation against AMD.
Flavie Courtaut; Alessandra Scagliarini; Virginie Aires; Clarisse Cornebise; Jean-Paul Pais de Barros; Céline Olmiere; Dominique Delmas. VEGF-R2/Caveolin-1 Pathway of Undifferentiated ARPE-19 Retina Cells: A Potential Target as Anti-VEGF-A Therapy in Wet AMD by Resvega, an Omega-3/Polyphenol Combination. International Journal of Molecular Sciences 2021, 22, 6590 .
AMA StyleFlavie Courtaut, Alessandra Scagliarini, Virginie Aires, Clarisse Cornebise, Jean-Paul Pais de Barros, Céline Olmiere, Dominique Delmas. VEGF-R2/Caveolin-1 Pathway of Undifferentiated ARPE-19 Retina Cells: A Potential Target as Anti-VEGF-A Therapy in Wet AMD by Resvega, an Omega-3/Polyphenol Combination. International Journal of Molecular Sciences. 2021; 22 (12):6590.
Chicago/Turabian StyleFlavie Courtaut; Alessandra Scagliarini; Virginie Aires; Clarisse Cornebise; Jean-Paul Pais de Barros; Céline Olmiere; Dominique Delmas. 2021. "VEGF-R2/Caveolin-1 Pathway of Undifferentiated ARPE-19 Retina Cells: A Potential Target as Anti-VEGF-A Therapy in Wet AMD by Resvega, an Omega-3/Polyphenol Combination." International Journal of Molecular Sciences 22, no. 12: 6590.
Eye diseases are currently a major public health concern due to the growing number of cases resulting from both an aging of populations and exogenous factors linked to our lifestyles. Thus, many treatments including surgical pharmacological approaches have emerged, and special attention has been paid to prevention, where diet plays a preponderant role. Recently, potential antioxidants such as resveratrol have received much attention as potential tools against various ocular diseases. In this review, we focus on the mechanisms of resveratrol against ocular diseases, in particular age-related macular degeneration, glaucoma, cataract, diabetic retinopathy, and vitreoretinopathy. We analyze, in relation to the different steps of each disease, the resveratrol properties at multiple levels, such as cellular and molecular signaling as well as physiological effects. We show and discuss the relationship to reactive oxygen species, the regulation of inflammatory process, and how resveratrol can prevent ocular diseases through a potential epigenetic action by the activation of sirtuin-1. Lastly, various new forms of resveratrol delivery are emerging at the same time as some clinical trials are raising more questions about the future of resveratrol as a potential tool for prevention or in therapeutic strategies against ocular diseases. More preclinical studies are required to provide further insights into RSV’s potential adjuvant activity.
Dominique Delmas; Clarisse Cornebise; Flavie Courtaut; Jianbo Xiao; Virginie Aires. New Highlights of Resveratrol: A Review of Properties against Ocular Diseases. International Journal of Molecular Sciences 2021, 22, 1295 .
AMA StyleDominique Delmas, Clarisse Cornebise, Flavie Courtaut, Jianbo Xiao, Virginie Aires. New Highlights of Resveratrol: A Review of Properties against Ocular Diseases. International Journal of Molecular Sciences. 2021; 22 (3):1295.
Chicago/Turabian StyleDominique Delmas; Clarisse Cornebise; Flavie Courtaut; Jianbo Xiao; Virginie Aires. 2021. "New Highlights of Resveratrol: A Review of Properties against Ocular Diseases." International Journal of Molecular Sciences 22, no. 3: 1295.
Age-related macular degeneration (AMD) is a degenerative disease of the retina where the molecular mechanism involves the production of vascular endothelial growth factor (VEGF), a factor of poor prognosis of the progression of the disease. Previous studies have shown that resveratrol, a polyphenol of grapevines, can prevent VEGF secretion induced by stress from retinal cells. Considering the fundamental role played by VEGF in development and progression of AMD, we investigate the potential effect of red wine extract (RWE) on VEGF secretion and its signaling pathway in human retinal cells ARPE-19. To examine the effect of RWE in ARPE-19, a quantitative and qualitative analysis of the RWE was performed by HPLC MS/MS. We show for the first time that RWE decreased VEGF-A secretion from ARPE-19 cells and its protein expression in concentration-dependent manner. RWE-induced alteration in VEGF-A production is associated with a down of VEGF-receptor 2 (VEGF-R2) protein expression and its phosphorylated intracytoplasmic domain. Subsequently, the activation of kinase pathway is disturbing and RWE prevents the phosphorylation of MEK and ERK 1/2 in human retinal cells ARPE-19. Finally, this study sheds light on the interest that the use of polyphenolic cocktails could represent in a prevention strategy.
Clarisse Cornebise; Flavie Courtaut; Marie Taillandier-Coindard; Josep Valls-Fonayet; Tristan Richard; David Monchaud; Virginie Aires; Dominique Delmas. Red Wine Extract Inhibits VEGF Secretion and Its Signaling Pathway in Retinal ARPE-19 Cells to Potentially Disrupt AMD. Molecules 2020, 25, 5564 .
AMA StyleClarisse Cornebise, Flavie Courtaut, Marie Taillandier-Coindard, Josep Valls-Fonayet, Tristan Richard, David Monchaud, Virginie Aires, Dominique Delmas. Red Wine Extract Inhibits VEGF Secretion and Its Signaling Pathway in Retinal ARPE-19 Cells to Potentially Disrupt AMD. Molecules. 2020; 25 (23):5564.
Chicago/Turabian StyleClarisse Cornebise; Flavie Courtaut; Marie Taillandier-Coindard; Josep Valls-Fonayet; Tristan Richard; David Monchaud; Virginie Aires; Dominique Delmas. 2020. "Red Wine Extract Inhibits VEGF Secretion and Its Signaling Pathway in Retinal ARPE-19 Cells to Potentially Disrupt AMD." Molecules 25, no. 23: 5564.