It is essential to study the characteristics of land use change in the Fuxian Lake basin, a plateau lake in Yunnan Province, and to predict the basin’s future trend of land use change for sustaining the key carriers of current national major strategies such as ecological civilization, green development, and rural revitalization. This study used the Fuxian Lake basin as the subject and based on the seven periods of historical land use data, added six driving factors including human and natural factors, applied patches to generate the land-use simulation model (PLUS), and forecasted and analyzed the characteristics of land-use change in the basin in 2048 under the three scenarios of natural trend development, ecological protection, and production protection. The results showed that: (1) the overall simulation accuracy of the model was 79.14%, Kappa index was 0.73, FOM value was 0.29, and the model’s consistency was high. The model can be used to simulate future land-use changes in the Fuxian Lake basin. (2) In the natural development scenario, land-use development and the driving factors in the basin have the following relationship: rainfall and trunk road distance significantly impacted the types of land that emphasize ecological conservation and product development. Elevation and soil distribution characteristics had a significant impact on land types focused mainly on water zones and ecological protection. The land types mainly focused on urban construction were greatly affected by elevation, trunk road distance, GDP per capita, and other factors. (3) The main direction of land-use change in the watershed is the mutual conversion between farmland and forest land, with the continuous expansion of construction land. In the production protection scenario, the area of farmland increased by 44.79 hm². In the ecological protection scenario, the area of arbor forest land increased by 37.85 hm² and the area of shrub forest land decreased by 62.37 hm². (4) From the perspective of spatial distribution patterns, the regional hotspot change blocks are mainly concentrated in the north of the basin, along the coast of the north of Fuxian Lake, and the southern land. In general, the PLUS model had good applicability in this study. The simulation results of the different scenarios were in line with the land development in the Fuxian Lake basin and can provide scientific reference for land-space planning, ecological and production land constraints, and coordination of development in the Fuxian Lake basin.

Southwest China is an important route for invasive species. In this study, 49 invasive plants of 15 families and 41 genera were found within 373 grassland sampling sites of Guizhou Province, a typical karst mountainous region with a high invasion risk located in Southwest China. Invasive plants could be found within over 90% of the grassland sampling sites, and malignant invasive species were found in 60% of the sites. In about 30% of the sampling sites, more than one malignant species coexisted. The malignant invasive species were mainly distributed in the southwestern part of Guizhou Province. Their distribution patterns were affected by environmental and traffic factors; they preferred areas with low elevation, high temperature, high rainfall, high soil nutrient content, and traffic accessibility and could adversely affect plant cover and biomass. Conversely, seriously invasive species and other low-level invasive species had a positive or neutral effect on grassland communities. Therefore, the focus of invasive plant control measures should be on malignant invasive species. Specific control policies and practices, especially in areas with resource-rich environments and well-developed traffic networks, should be carried out to facilitate grassland ecosystem sustainability and to prevent the spread of invasive species to inland China.

Quantitatively estimating the grazing intensity (GI) effects on vegetation in semiarid hilly grassland of the Loess Plateau can help to develop safe utilization levels for natural grasslands, which is a necessity of maintaining livestock production and sustainable development of grasslands. Normalized difference vegetation index (NDVI), field vegetation data, and 181 days (one goat per day) of GPS tracking were combined to quantify the spatial pattern of GI, and its effects on the vegetation community structure. The spatial distribution of GI was uneven, with a mean value of 0.50 goats/ha, and 95% of the study area had less than 1.30 goats/ha. The areas with utilization rates of rangeland (July) lower than 45% and 20% made up about 95% and 60% of the study area, respectively. Grazing significantly reduced monthly aboveground biomass, but the grazing effects on plant growth rate were complex across the different plant growth stages. Grazing impaired plant growth in general, but the intermediate GI appeared to facilitate plant growth rate at the end of the growing seasons. Grazing had minimal relationship with vegetation community structure characteristics, though Importance Value of forbs increased with increasing GI. Flexibility in the number of goats and conservatively defining utilization rate, according to the inter-annual variation of utilization biomass, would be beneficial to achieve ecologically healthy and economically sustainable GI.

Understanding the molecular mechanism that underlies the differentiation of neural stem cells (NSCs) is vital to develop regenerative medicines for neurological disorders. In our previous work, Rho-GDI-γ was found to be able to prompt neuronal differentiation when it was down regulated. However, it is unclear how Rho-GDI-γ regulates this differentiation process. Therefore, a novel systems biology approach is presented here to identify putative signalling pathways regulated by Rho-GDI-γ during NSC differentiation, and these pathways can provide insights into the NSC differentiation mechanisms. In particular, our proposed approach combines the predictive power of computational biology and molecular experiments. With different biological experiments, the genes in the computationally identified signalling network were validated to be indeed regulated by Rho-GDI-γ during the differentiation of NSCs. In particular, one randomly selected pathway involving Vcp, Mapk8, Ywhae and Ywhah was experimentally verified to be regulated by Rho-GDI-γ. These promising results demonstrate the effectiveness of our proposed systems biology approach, indicating the potential predictive power of integrating computational and experimental approaches.