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Lasiodiplodan is a β-glucan polymer with different interesting characteristics, including therapeutic properties. It is an extracellular product, which is produced by the filamentous fungus Lasiodiplodia theobromae, using glucose as a substrate. In the present work, the production of lasiodiplodan was studied by the utilization of sugarcane straw as a low-cost carbon source. Glucose-rich sugarcane straw hydrolysate was obtained by a sequential pretreatment with dilute nitric acid (1% v/v) and sodium hydroxide (1% w/v), followed by enzymatic hydrolysis. The fermentation process was conducted by the cultivation of the strain Lasiodiplodia theobromae CCT3966 in sugarcane straw hydrolysate in a shake flask at 28 °C for 114 h. It was found that hydrolysate obtained after enzymatic hydrolysis contained 47.10 gL−1 of glucose. Fermentation experiments of lasiodiplodan synthesis showed that the peak yield and productivity of 0.054 gg−1 glucose consumed and 0.016 gL−1 h−1, respectively, were obtained at 72 h fermentation time. Fungal growth, glucose consumption, and lasiodiplodan production from sugarcane straw hydrolysate presented a similar pattern to kinetic models. The study on the chemical structure of lasiodiplodan produced showed it had a β-glucan construction. The current study revealed that sugarcane straw is a promising substrate for the production of lasiodiplodan.
Peyman Abdeshahian; Jesús Jiménez Ascencio; Rafael R. Philippini; Felipe Antonio Fernandes Antunes; Avinash P. Ingle; Mojgan Abdeshahian; Júlio César dos Santos; Silvio Silvério da Silva. Fermentative Production of Lasiodiplodan by Lasiodiplodia theobromae CCT3966 from Pretreated Sugarcane Straw. Sustainability 2021, 13, 9697 .
AMA StylePeyman Abdeshahian, Jesús Jiménez Ascencio, Rafael R. Philippini, Felipe Antonio Fernandes Antunes, Avinash P. Ingle, Mojgan Abdeshahian, Júlio César dos Santos, Silvio Silvério da Silva. Fermentative Production of Lasiodiplodan by Lasiodiplodia theobromae CCT3966 from Pretreated Sugarcane Straw. Sustainability. 2021; 13 (17):9697.
Chicago/Turabian StylePeyman Abdeshahian; Jesús Jiménez Ascencio; Rafael R. Philippini; Felipe Antonio Fernandes Antunes; Avinash P. Ingle; Mojgan Abdeshahian; Júlio César dos Santos; Silvio Silvério da Silva. 2021. "Fermentative Production of Lasiodiplodan by Lasiodiplodia theobromae CCT3966 from Pretreated Sugarcane Straw." Sustainability 13, no. 17: 9697.
Lasiodiplodan, a (1→6)-β-d-glucan, is an exopolysaccharide with high commercial value and many applications in food, pharmaceuticals, and cosmetics. Current industrial production of β-glucans from crops is mostly by chemical routes generating hazardous and toxic waste. Therefore, alternative sustainable and eco-friendly pathways are highly desirable. Here, we have studied the lasiodiplodan production from sugarcane bagasse (SCB), a major lignocellulosic agricultural residue, by Lasiodiplodia theobromae CCT 3966. Lasiodiplodan accumulated on SCB hydrolysate (carbon source) supplemented with soybean bran or rice bran (nitrogen source) was 16.2 [6.8 × 103 Da] and 22.0 [7.6 × 103 Da] g/L, respectively. Lasiodiplodan showed high purity, low solubility, pseudoplastic behavior and was composed of glucose units. Moreover, the exopolysaccharides were substantially amorphous with moderate thermal stability and similar degradation temperatures. To our knowledge, this is the first report on the highest production of SCB-based lasiodiplodan to date. L. theobromae, as a microbial cell factory, demonstrated the commercial potential for the sustainable production of lasiodiplodan from renewable biomass feedstock.
Jesús Ascencio; Rafael Philippini; Fabricio Gomes; Félix Pereira; Silvio da Silva; Vinod Kumar; Anuj Chandel. Comparative Highly Efficient Production of β-glucan by Lasiodiplodia theobromae CCT 3966 and Its Multiscale Characterization. Fermentation 2021, 7, 108 .
AMA StyleJesús Ascencio, Rafael Philippini, Fabricio Gomes, Félix Pereira, Silvio da Silva, Vinod Kumar, Anuj Chandel. Comparative Highly Efficient Production of β-glucan by Lasiodiplodia theobromae CCT 3966 and Its Multiscale Characterization. Fermentation. 2021; 7 (3):108.
Chicago/Turabian StyleJesús Ascencio; Rafael Philippini; Fabricio Gomes; Félix Pereira; Silvio da Silva; Vinod Kumar; Anuj Chandel. 2021. "Comparative Highly Efficient Production of β-glucan by Lasiodiplodia theobromae CCT 3966 and Its Multiscale Characterization." Fermentation 7, no. 3: 108.
The potential utilization of corn bran acid hydrolysate (CBAH) was evaluated as an inexpensive feedstock for the production of a rich carbohydrate and protein medium for lasiodiplodan (LAS) production using the filamentous fungus Lasiodiplodia theobromae CCT 3966. Experiments were performed according to a 22 CCRD experimental design aiming to evaluate the influence of agitation speed (rpm) and temperature (°C) over the production of total cell biomass (TCB) and LAS concentration released to the medium (LAS-M), adhered to biomass (LAS-C), and total (LAS-T). Under the selected conditions (temperature of 28°C and agitation of 200 rpm), 8.73 g·L−1 of LAS-T and 4.47 g·L−1 of TCB were obtained. Recovery of LAS-C with hot water was shown as an alternative to increase the production concentration, although it might require further purification steps. CBAH potential for substitution of synthetic media was demonstrated, indicating that it is an adequate raw material containing all necessary nutrients for LAS production. Key points • Corn bran acid hydrolysate is presented as a suitable substrate for β-glucan production. • Lasiodiplodia theobromae CCT 3966 have the potential for the industrial β-glucan production. • Simple recovering of biomass-adhered lasiodiplodan by hot water extraction.
Rafael Rodrigues Philippini; Sabrina Evelin Martiniano; Paulo Ricardo Franco Marcelino; Anuj Kumar Chandel; Júlio César dos Santos; Silvio Silvério Da Silva. Production of β-glucan exopolysaccharide lasiodiplodan by Lasiodiplodia theobromae CCT 3966 from corn bran acid hydrolysate. Applied Microbiology and Biotechnology 2021, 105, 2319 -2332.
AMA StyleRafael Rodrigues Philippini, Sabrina Evelin Martiniano, Paulo Ricardo Franco Marcelino, Anuj Kumar Chandel, Júlio César dos Santos, Silvio Silvério Da Silva. Production of β-glucan exopolysaccharide lasiodiplodan by Lasiodiplodia theobromae CCT 3966 from corn bran acid hydrolysate. Applied Microbiology and Biotechnology. 2021; 105 (6):2319-2332.
Chicago/Turabian StyleRafael Rodrigues Philippini; Sabrina Evelin Martiniano; Paulo Ricardo Franco Marcelino; Anuj Kumar Chandel; Júlio César dos Santos; Silvio Silvério Da Silva. 2021. "Production of β-glucan exopolysaccharide lasiodiplodan by Lasiodiplodia theobromae CCT 3966 from corn bran acid hydrolysate." Applied Microbiology and Biotechnology 105, no. 6: 2319-2332.
The production of biomolecules using agro-industrial by-products as feedstock is a growing trend worldwide. Selenium (Se) is a trace element essential for health, and the Se-enrichment of yeast biomass can enhance its benefits. This study investigated the feasibility of the production of Saccharomyces cerevisiae Se-enriched biomass using a medium composed of corn bran and soybean bran acid hydrolysates as carbon and nitrogen sources in a stirred-tank reactor. After hydrolysis, hydrolysates presented complex composition and high concentrations of sugars, proteins, and minerals. The use of a stirred-tank bioreactor leads to the production of 9 g/L S. cerevisiae biomass enriched with 236.93 μg/g Se, and 99% cell viability. Likewise, the combination of sugarcane molasses and soybean bran hydrolysate was effective for cell growth of a probiotic strain of S. cerevisiae with a 24.08% β-glucan content. The results demonstrated that starchy acid hydrolysates are low-cost and efficient substrates for the production of yeast biomass and derivate products and may contribute to further studies for a sustainable development of biorefinery technologies.
Sabrina Evelin Martiniano; Letícia Alves Fernandes; Edith Mier Alba; Rafael Rodrigues Philippini; Stephanie Caroline Tavares Tabuchi; Marek Kieliszek; Júlio César Dos Santos; Silvio Silvério Da Silva. A New Approach for the Production of Selenium-Enriched and Probiotic Yeast Biomass from Agro-Industrial by-Products in a Stirred-Tank Bioreactor. Metabolites 2020, 10, 508 .
AMA StyleSabrina Evelin Martiniano, Letícia Alves Fernandes, Edith Mier Alba, Rafael Rodrigues Philippini, Stephanie Caroline Tavares Tabuchi, Marek Kieliszek, Júlio César Dos Santos, Silvio Silvério Da Silva. A New Approach for the Production of Selenium-Enriched and Probiotic Yeast Biomass from Agro-Industrial by-Products in a Stirred-Tank Bioreactor. Metabolites. 2020; 10 (12):508.
Chicago/Turabian StyleSabrina Evelin Martiniano; Letícia Alves Fernandes; Edith Mier Alba; Rafael Rodrigues Philippini; Stephanie Caroline Tavares Tabuchi; Marek Kieliszek; Júlio César Dos Santos; Silvio Silvério Da Silva. 2020. "A New Approach for the Production of Selenium-Enriched and Probiotic Yeast Biomass from Agro-Industrial by-Products in a Stirred-Tank Bioreactor." Metabolites 10, no. 12: 508.
The integrated approach in biorefinery mainly involves the utilization of various agroindustrial byproducts such as raw materials for the production of several biobased products like biofuels, bioenergy, and other high-value chemicals. Biofuels are the backbone of biorefineries, however, production of value-added biomolecules such as biopigments, biopolymers, biosurfactants, and nutritional yeast has been attracting great attention. The production of these biomolecules using traditional approaches has been extensively studied in the last few years owing to their promising application in different industries such as chemical, food/feed, and pharmaceuticals for the development of novel products for mankind. Moreover, the production of such biomolecules using lignocellulosic, starchy, and some other agroindustrial byproducts is still not fully explored. Hence, there is a huge scope in the development of sustainable biorefining approaches to make the technology cost-effective. The lignocellulosic biomasses usually used in biorefineries are mainly composed of cellulose, hemicellulose, and lignin, whereas starchy materials, besides starch, usually contain, protein, lipids, and some micronutrients. The processing of these biomasses through successive steps like pretreatments, enzymatic hydrolysis, and fermentation is essentially required to obtained final biobased products. Considering certain bottlenecks of above-mentioned conventional biorefineries approaches, new technologies have been proposed for the improved pretreatment of biomass and efficient enzymatic hydrolysis in order to minimize the concentration of toxic inhibitors in resulting hydrolysate. In this review, we highlighted the different agroindustrial byproducts and their applications for the production of valuable biorefinery products.
Rafael Rodrigues Philippini; Sabrina Evelin Martiniano; Avinash P. Ingle; Paulo Ricardo Franco Marcelino; Gilda Mariano Silva; Fernanda Gonçalves Barbosa; Júlio Santos; Silvio Silvério Da Silva. Agroindustrial Byproducts for the Generation of Biobased Products: Alternatives for Sustainable Biorefineries. Frontiers in Energy Research 2020, 8, 1 .
AMA StyleRafael Rodrigues Philippini, Sabrina Evelin Martiniano, Avinash P. Ingle, Paulo Ricardo Franco Marcelino, Gilda Mariano Silva, Fernanda Gonçalves Barbosa, Júlio Santos, Silvio Silvério Da Silva. Agroindustrial Byproducts for the Generation of Biobased Products: Alternatives for Sustainable Biorefineries. Frontiers in Energy Research. 2020; 8 ():1.
Chicago/Turabian StyleRafael Rodrigues Philippini; Sabrina Evelin Martiniano; Avinash P. Ingle; Paulo Ricardo Franco Marcelino; Gilda Mariano Silva; Fernanda Gonçalves Barbosa; Júlio Santos; Silvio Silvério Da Silva. 2020. "Agroindustrial Byproducts for the Generation of Biobased Products: Alternatives for Sustainable Biorefineries." Frontiers in Energy Research 8, no. : 1.
The use of agro-industrial by-products as feedstock for selenium-enriched yeast biomass production is an innovative and low-cost method. Thus, the present study aims for the utilization of sugarcane bagasse and corn bran hydrolysates for the production of selenium-enriched yeast and the evaluation of the effect of selenium in growth and cell composition. The hydrolysates were obtained using acid pretreatment, and yeasts were evaluated in different medium compositions. All evaluated yeasts were able to grow in the presence of 15 mg/L selenium. S. cerevisiae strains presented the major tolerance to selenium and better relation between selenium uptake and cell growth, with maximum cell biomass production of 7.97 ± 0.24 g/L and maximum selenium uptake of 1193 ± 336 ppm and 99% cell viability. Selenium also enhanced the lipid and protein concentration in cell extracts obtained after mechanical disruption. Regarding agro-industrial hydrolysates, corn bran hydrolysate allowed a better growth, with the production of 4.25 ± 0.49 g/L of biomass enriched with 167 ± 18 ppm selenium and 100% of viability. Starchy and lignocellulosic biomasses presented potential as low-cost alternatives for single-cell protein contributing to the development of sustainable and economically viable technologies. Graphical abstract
Sabrina Evelin Martiniano; Rafael Philippini; Paulo Ricardo Franco-Marcelino; Silvio Silvério Da Silva. Effect of selenium uptake on growth metabolism in yeasts for the production of enriched single-cell protein using agro-industrial by-products. Biomass Conversion and Biorefinery 2020, 1 -9.
AMA StyleSabrina Evelin Martiniano, Rafael Philippini, Paulo Ricardo Franco-Marcelino, Silvio Silvério Da Silva. Effect of selenium uptake on growth metabolism in yeasts for the production of enriched single-cell protein using agro-industrial by-products. Biomass Conversion and Biorefinery. 2020; ():1-9.
Chicago/Turabian StyleSabrina Evelin Martiniano; Rafael Philippini; Paulo Ricardo Franco-Marcelino; Silvio Silvério Da Silva. 2020. "Effect of selenium uptake on growth metabolism in yeasts for the production of enriched single-cell protein using agro-industrial by-products." Biomass Conversion and Biorefinery , no. : 1-9.
β-Glucans as emerging biopolymer are widely produced by microorganisms in fermentation processes using commercial sugars which make process non-economic. Lignocellulosic substances are inexpensive carbon sources, which could be exploited for sustainable production of β-glucans. In this study, a lignocellulosic material, namely sugarcane straw (SCS) was utilized for the production of extracellular β-glucan by Lasiodiplodia theobromae CCT3966. SCS was subjected to acid and subsequent alkaline pretreatment, followed by enzymatic saccharification using cellulase enzyme. Quantity of 48.65 g/L glucose was released after enzymatic hydrolysis. β-Glucan production was performed by cultivation of fungal strain in SCS hydrolysate at 28 °C and initial culture pH 7. Highest β-glucan yield and productivity of 0.047 gg−1 and 0.014 gL-1h−1, respectively was obtained at 72 h fermentation time. Kinetic study of β-glucan production revealed experimental biosynthesis of β-glucan from SCS hydrolysate followed the trend generated by Logistic and Luedeking-Piret models. Chemical structure of biopolymer produced showed β-glucan constitution.
Peyman Abdeshahian; Jesús Jiménez Ascencio; Rafael Philippini; Felipe Antonio Fernandes Antunes; Júlio Santos; Silvio Silvério da Silva. Utilization of sugarcane straw for production of β-glucan biopolymer by Lasiodiplodia theobromae CCT 3966 in batch fermentation process. Bioresource Technology 2020, 314, 123716 .
AMA StylePeyman Abdeshahian, Jesús Jiménez Ascencio, Rafael Philippini, Felipe Antonio Fernandes Antunes, Júlio Santos, Silvio Silvério da Silva. Utilization of sugarcane straw for production of β-glucan biopolymer by Lasiodiplodia theobromae CCT 3966 in batch fermentation process. Bioresource Technology. 2020; 314 ():123716.
Chicago/Turabian StylePeyman Abdeshahian; Jesús Jiménez Ascencio; Rafael Philippini; Felipe Antonio Fernandes Antunes; Júlio Santos; Silvio Silvério da Silva. 2020. "Utilization of sugarcane straw for production of β-glucan biopolymer by Lasiodiplodia theobromae CCT 3966 in batch fermentation process." Bioresource Technology 314, no. : 123716.
The ubiquitous nature of lignocellulosic biomass on planet earth and its economic viability attracted a great deal of attention from researchers and becomes foremost feedstock for biofuel production particularly bioethanol. However, due to complexity in structure, its pretreatment is essentially required prior to actual use. In the present study, a promising approach has been proposed through the development of acid-functionalized magnetic nanocatalysts. Two different acid-functionalized magnetic nanocatalysts i.e. alkylsulfonic acid functionalized magnetic nanoparticles (Fe3O4-MNPs-Si-AS) and butylcarboxylic acid functionalized magnetic nanoparticles (Fe3O4-MNPs-Si-BCOOH) were developed and their efficacy was studied in the pretreatment of sugarcane straw at varying concentrations (100, 200, 300, 400, 500 mg/g of straw). The enhanced concentration dependent production of sugar (xylose) was reported in case of both the nanocatalysts. The maximum 17.06 g/L for Fe3O4-MNPs-Si-AS and 15.40 g/L for Fe3O4-MNPs-Si-BCOOH sugar was reported at 500 mg which is comparatively higher than normal acid (H2SO4) (14.63 g/L) and non-treated (0.24 g/L) sugarcane straw. Further, both the nanocatalysts were recovered by applying an external magnetic field and reused for the next two subsequent cycles of pretreatment. It was observed that with every reuse of nanocatalysts the concentration of sugar production was reduced. Moreover, generation of very less amount of toxic inhibitors was reported in the hemicellulosic hydrolyzate obtained in the present study. Considering these facts, it is believed that such nanocatalysts can be used as an effective, eco-friendly and economically viable alternative to the conventional pretreatment agents like mineral acids.
Avinash P. Ingle; Rafael Philippini; Yasmin Cristhine De Souza Melo; Silvio Silvério Da Silva. Acid-functionalized magnetic nanocatalysts mediated pretreatment of sugarcane straw: an eco-friendly and cost-effective approach. Cellulose 2020, 27, 7067 -7078.
AMA StyleAvinash P. Ingle, Rafael Philippini, Yasmin Cristhine De Souza Melo, Silvio Silvério Da Silva. Acid-functionalized magnetic nanocatalysts mediated pretreatment of sugarcane straw: an eco-friendly and cost-effective approach. Cellulose. 2020; 27 (12):7067-7078.
Chicago/Turabian StyleAvinash P. Ingle; Rafael Philippini; Yasmin Cristhine De Souza Melo; Silvio Silvério Da Silva. 2020. "Acid-functionalized magnetic nanocatalysts mediated pretreatment of sugarcane straw: an eco-friendly and cost-effective approach." Cellulose 27, no. 12: 7067-7078.
The excessive consumption of petroleum resources leads to global warming, fast depletion of petroleum reserves, as well as price instability of gasoline. Thus, there is a strong need for alternative renewable fuels to replace petroleum-derived fuels. The striking features of an alternative fuel include the low carbon footprints, renewability and affordability at manageable prices. Biodiesel, made from waste oils, animal fats, vegetal oils, is a totally renewable and non-toxic liquid fuel which has gained significant attraction in the world. Due to technological advancements in catalytic chemistry, biodiesel can be produced from a variety of feedstock employing a variety of catalysts and recovery technologies. Recently, several ground-breaking advancements have been made in nano-catalyst technology which showed the symmetrical correlation with cost competitive biodiesel production. Nanocatalysts have unique properties such as their selective reactivity, high activation energy and controlled rate of reaction, easy recovery and recyclability. Here, we present an overview of various feedstock used for biodiesel production, their composition and characteristics. The major focus of this review is to appraise the characterization of nanocatalysts, their effect on biodiesel production and methodologies of biodiesel production.
Avinash P. Ingle; Anuj K. Chandel; Rafael Philippini; Sabrina Evelin Martiniano; Silvio Silvério Da Silva. Advances in Nanocatalysts Mediated Biodiesel Production: A Critical Appraisal. Symmetry 2020, 12, 256 .
AMA StyleAvinash P. Ingle, Anuj K. Chandel, Rafael Philippini, Sabrina Evelin Martiniano, Silvio Silvério Da Silva. Advances in Nanocatalysts Mediated Biodiesel Production: A Critical Appraisal. Symmetry. 2020; 12 (2):256.
Chicago/Turabian StyleAvinash P. Ingle; Anuj K. Chandel; Rafael Philippini; Sabrina Evelin Martiniano; Silvio Silvério Da Silva. 2020. "Advances in Nanocatalysts Mediated Biodiesel Production: A Critical Appraisal." Symmetry 12, no. 2: 256.
The bioconversion of lignocellulosic biomass to second generation (2G) sugars is crucial for the production of biofuels and commodity chemicals. However, due to its recalcitrant nature, pretreatment is an essential step to increase the accessibility of cellulolytic enzymes to hemicellulose and cellulose to obtain 2G sugars. In this study, sugarcane bagasse (SCB) was pretreated by dilute nitric acid (1% w/v), dilute sodium hydroxide (1% w/v) and sequential nitric acid-sodium hydroxide. The pretreated material was then enzymatically hydrolysed (5 and 10% total solids; TS) by cellulase (Cellic Ctec 2, Novozyme, Curitiba, Brazil). Sequential acid–base pretreated bagasse (cellulosic pulp) led the removal of lignin (70.63%) and hemicellulose (100%) and retained 92.33% cellulose. Enzymatic hydrolysis of sequential acid–base pretreated bagasse (5% TS) showed hydrolysis yield of 75.68% (glucose released), followed by sodium hydroxide pretreated with glucose (68.76%) and xylose (73.26%) and nitric acid pretreated with glucose (31.49%) and xylose (31.49%), respectively. Enzymatic hydrolysis of sequential acid–base pretreated (10% TS) showed hydrolysis yield of 66.20% (glucose), followed by glucose (63.02%) and xylose (60.14%) from sodium hydroxide pretreatment and finally glucose (28.71%) and xylose (23.56%) from dilute nitric acid pretreatment. Therefore, the cellulosic material showed high hydrolysis efficiency after enzymatic saccharification, proving that sequential removal of hemicellulose and lignin from the biomass enables high accessibility of cellulases to the substrate, eventually yielding high amount of 2G sugars.
Jesús J. Ascencio; Anuj K. Chandel; Rafael Philippini; Silvio S. Da Silva. Comparative study of cellulosic sugars production from sugarcane bagasse after dilute nitric acid, dilute sodium hydroxide and sequential nitric acid-sodium hydroxide pretreatment. Biomass Conversion and Biorefinery 2019, 10, 813 -822.
AMA StyleJesús J. Ascencio, Anuj K. Chandel, Rafael Philippini, Silvio S. Da Silva. Comparative study of cellulosic sugars production from sugarcane bagasse after dilute nitric acid, dilute sodium hydroxide and sequential nitric acid-sodium hydroxide pretreatment. Biomass Conversion and Biorefinery. 2019; 10 (4):813-822.
Chicago/Turabian StyleJesús J. Ascencio; Anuj K. Chandel; Rafael Philippini; Silvio S. Da Silva. 2019. "Comparative study of cellulosic sugars production from sugarcane bagasse after dilute nitric acid, dilute sodium hydroxide and sequential nitric acid-sodium hydroxide pretreatment." Biomass Conversion and Biorefinery 10, no. 4: 813-822.
Pretreatment is one of the most important steps in the production of bioethanol from renewable feedstocks like lignocellulosic biomass, however, existing pretreatment approaches have some limitations. In this context, two different acid-functionalized magnetic nanoparticles (MNPs) i.e. alkylsulfonic acid (Fe3O4[email protected]@AS) and butylcarboxylic acid (Fe3O4[email protected]@BCOOH) were synthesized and evaluated for their efficacy at different concentration in the pretreatment of sugarcane bagasse. It was observed that both of these acid-functionalized MNPs showed concentration-dependent promising catalytic activity as compared to conventional acid pretreatment. Both Fe3O4[email protected]@AS and Fe3O4[email protected]@BCOOH at 500 mg/g of bagasse showed the maximum amount of sugar (xylose) liberated i.e. 18.83 g/L and 18.67 g/L, respectively which are comparatively higher than the normal acid pretreatment (15.40 g/L) and untreated sample (0.28 g/L). Further, both the acid-functionalized MNPs used were recovered by applying magnetic field and reused for next two subsequent cycles of pretreatment. Therefore, such nanotechnology-based approaches can be used as a rapid and eco-friendly alternative method for the pretreatment of a variety of lignocellulosic materials. Moreover, the reuse of the same MNPs for more than one cycle of pretreatment can also help to reduce the cost involved in the process.
Avinash P. Ingle; Rafael Philippini; Silvio Silvério da Silva. Pretreatment of sugarcane bagasse using two different acid-functionalized magnetic nanoparticles: A novel approach for high sugar recovery. Renewable Energy 2019, 150, 957 -964.
AMA StyleAvinash P. Ingle, Rafael Philippini, Silvio Silvério da Silva. Pretreatment of sugarcane bagasse using two different acid-functionalized magnetic nanoparticles: A novel approach for high sugar recovery. Renewable Energy. 2019; 150 ():957-964.
Chicago/Turabian StyleAvinash P. Ingle; Rafael Philippini; Silvio Silvério da Silva. 2019. "Pretreatment of sugarcane bagasse using two different acid-functionalized magnetic nanoparticles: A novel approach for high sugar recovery." Renewable Energy 150, no. : 957-964.
The present study demonstrated the preparation of three different acid-functionalised magnetic nanoparticles (MNPs) and evaluation for their catalytic efficacy in hydrolysis of cellobiose. Initially, iron oxide (Fe3 O4)MNPs were synthesised, which further modified by applying silica coating (Fe3 O4 [email protected]) and functionalised with alkylsulfonic acid (Fe3 O4 [email protected]@AS), butylcarboxylic acid (Fe3 O4 [email protected]@BCOOH) and sulphonic acid (Fe3 O4 [email protected]@SO3 H) groups. The Fourier transform infrared analysis confirmed the presence of above-mentioned acid functional groups on MNPs. Similarly, X-ray diffraction pattern and energy dispersive X-ray spectroscopy analysis confirmed the crystalline nature and elemental composition of MNPs, respectively. TEM micrographs showed the synthesis of spherical and polydispersed nanoparticles having diameter size in the range of 20–80 nm. Cellobiose hydrolysis was used as a model reaction to evaluate the catalytic efficacy of acid-functionalised nanoparticles. A maximum 74.8% cellobiose conversion was reported in case of Fe3 O4 [email protected]@SO3 H in first cycle of hydrolysis. Moreover, thus used acid-functionalised MNPs were magnetically separated and reused. In second cycle of hydrolysis, Fe3 O4 [email protected]@SO3 H showed 49.8% cellobiose conversion followed by Fe3 O4 [email protected]@AS (45%) and Fe3 O4 [email protected]@BCOOH (18.3%). However, similar pattern was reported in case of third cycle of hydrolysis. The proposed approach is considered as rapid and convenient. Moreover, reuse of acid-functionalised MNPs makes the process economically viable.
Avinash P. Ingle; Rafael R. Philippini; Mahendra Rai; Silvio Silvério da Silva. Catalytic hydrolysis of cellobiose using different acid‐functionalised Fe 3 O 4 magnetic nanoparticles. IET Nanobiotechnology 2019, 14, 40 -46.
AMA StyleAvinash P. Ingle, Rafael R. Philippini, Mahendra Rai, Silvio Silvério da Silva. Catalytic hydrolysis of cellobiose using different acid‐functionalised Fe 3 O 4 magnetic nanoparticles. IET Nanobiotechnology. 2019; 14 (1):40-46.
Chicago/Turabian StyleAvinash P. Ingle; Rafael R. Philippini; Mahendra Rai; Silvio Silvério da Silva. 2019. "Catalytic hydrolysis of cellobiose using different acid‐functionalised Fe 3 O 4 magnetic nanoparticles." IET Nanobiotechnology 14, no. 1: 40-46.
Cell wall composition in lignocellulosic biomass varies depending on genetic origin, growth conditions, weather and soil conditions. Here, we have evaluated the chemical characterization, morphology and enzymatic hydrolysis efficiencies (after sequential dilute acid-base pretreatment) of sugarcane bagasse from five different hybrid varieties of sugarcane. On average, bagasse samples showed cellulose, hemicellulose, lignin and ash compositions of 40.84, 24.07, 33.71 and 0.68%, respectively. Sequential acid-base pretreatment removed approximately 77 and 58% hemicellulose and lignin, respectively, leaving pulp samples rich in cellulose (up to 80%), which exhibited a maximum saccharification yields of 55.39% after enzymatic hydrolysis. We found average contents of cellulose (54.17 and 77.48%), hemicellulose (5.64 and 6.07%), lignin (37.28 and 15.40%) and ash (0.54 and 0.32%) in cellulignin and cellulosic pulp, respectively. Results showed that the genetic variability of sugarcane had no influence on the chemical composition and sugar recovery after saccharification of bagasse samples. Therefore, sugarcane bagasse from these new sugarcane varieties samples may be used for second generation sugars production. Cellulosic sugars may serve as primary building block for renewable fuels and chemicals production at commercial scale under biorefinery concept.
Rafael Philippini; Sabrina E. Martiniano; Anuj K. Chandel; Walter De Carvalho; Silvio S. Da Silva. Pretreatment of Sugarcane Bagasse from Cane Hybrids: Effects on Chemical Composition and 2G Sugars Recovery. Waste and Biomass Valorization 2017, 10, 1561 -1570.
AMA StyleRafael Philippini, Sabrina E. Martiniano, Anuj K. Chandel, Walter De Carvalho, Silvio S. Da Silva. Pretreatment of Sugarcane Bagasse from Cane Hybrids: Effects on Chemical Composition and 2G Sugars Recovery. Waste and Biomass Valorization. 2017; 10 (6):1561-1570.
Chicago/Turabian StyleRafael Philippini; Sabrina E. Martiniano; Anuj K. Chandel; Walter De Carvalho; Silvio S. Da Silva. 2017. "Pretreatment of Sugarcane Bagasse from Cane Hybrids: Effects on Chemical Composition and 2G Sugars Recovery." Waste and Biomass Valorization 10, no. 6: 1561-1570.
Biosurfactants are microbial metabolites with possible applications in various industrial sectors that are considered ecofriendly molecules. In recent years, some studies identified these compounds as alternatives for the elimination of vectors of tropical diseases, such as Aedes aegypti. The major bottlenecks of biosurfactant industrial production have been the use of conventional raw materials that increase production costs as well as opportunistic or pathogenic bacteria, which restrict the application of these biomolecules. The present study shows the potential of hemicellulosic sugarcane bagasse hydrolysate as a raw material for the production of a crystalline glycolipidic BS by Scheffersomyces stipitis NRRL Y-7124, which resulted in an emulsifying index (EI24) of 70 ± 3.4% and a superficial tension of 52 ± 2.9 mN.m-1. Additionally, a possible new application of these compounds as biolarvicides, mainly against A. aegypti, was evaluated. At a concentration of 800 mg.L-1, the produced biosurfactant caused destruction to the larval exoskeletons 12 h after application and presented an letal concentration (LC50) of 660 mg.L-1. Thus, a new alternative for biosurfactant production using vegetal biomass as raw material within the concept of biorefineries was proposed, and the potential of the crystalline glycolipidic biosurfactant in larvicidal formulations against neglected tropical disease vectors was demonstrated.
Paulo Ricardo Franco Marcelino; Vinícius Luiz Da Silva; Rafael Philippini; Cláudio José Von Zuben; Jonas Contiero; Júlio Santos; Silvio Silvério da Silva. Biosurfactants produced by Scheffersomyces stipitis cultured in sugarcane bagasse hydrolysate as new green larvicides for the control of Aedes aegypti, a vector of neglected tropical diseases. PLOS ONE 2017, 12, e0187125 .
AMA StylePaulo Ricardo Franco Marcelino, Vinícius Luiz Da Silva, Rafael Philippini, Cláudio José Von Zuben, Jonas Contiero, Júlio Santos, Silvio Silvério da Silva. Biosurfactants produced by Scheffersomyces stipitis cultured in sugarcane bagasse hydrolysate as new green larvicides for the control of Aedes aegypti, a vector of neglected tropical diseases. PLOS ONE. 2017; 12 (11):e0187125.
Chicago/Turabian StylePaulo Ricardo Franco Marcelino; Vinícius Luiz Da Silva; Rafael Philippini; Cláudio José Von Zuben; Jonas Contiero; Júlio Santos; Silvio Silvério da Silva. 2017. "Biosurfactants produced by Scheffersomyces stipitis cultured in sugarcane bagasse hydrolysate as new green larvicides for the control of Aedes aegypti, a vector of neglected tropical diseases." PLOS ONE 12, no. 11: e0187125.
Xylose is the main sugar in hemicellulosic hydrolysates and its fermentation into ethanol by microorganisms is influenced by nutritional factors, such as nitrogen source, vitamins and other elements. Rice bran extract (RBE) is an inexpensive nitrogen source primarily consisting of high amount of protein. This study evaluates the potential of RBE as a nitrogen source for the “hemicellulosic ethanol” production from sugarcane bagasse dilute acid hydrolysate by novel yeast strains Scheffersomyces shehatae (syn. Candida shehatae) CG8-8BY and Spathaspora arborariae UFMG-HM19.1A, isolated from Brazilian forests. Two different media formulations were used for inoculum preparation and production medium, using yeast extract and RBE as nitrogen sources. S. shehatae CG8-8BY showed ethanol production of 17.0 g/l with the ethanol yield (0.33 g/g) and fermentation efficiency (64 %) from medium supplemented with RBE. On the other hand, S. arborariae presented 5.4 g/l of ethanol production with ethanol yield (0.14 g/g) and fermentation efficiency (21 %) in a fermentation medium supplemented with RBE. Appropriate media formulation is an important parameter to increase the productivity of bioconversion process and RBE proved to be an efficient and inexpensive nitrogen source to supplement sugarcane bagasse hemicellulosic hydrolysate for second generation ethanol production.
Sabrina E. Martiniano; Rafael Philippini; Anuj K. Chandel; Carlos A. Rosa; Fernando C. Pagnocca; Silvio Silvério Da Silva. Evaluation of Rice Bran Extract as a Nitrogen Source for Improved Hemicellulosic Ethanol Production from Sugarcane Bagasse by New Xylose-Fermenting Yeast Strains Isolated from Brazilian Forests. Sugar Tech 2013, 16, 1 -8.
AMA StyleSabrina E. Martiniano, Rafael Philippini, Anuj K. Chandel, Carlos A. Rosa, Fernando C. Pagnocca, Silvio Silvério Da Silva. Evaluation of Rice Bran Extract as a Nitrogen Source for Improved Hemicellulosic Ethanol Production from Sugarcane Bagasse by New Xylose-Fermenting Yeast Strains Isolated from Brazilian Forests. Sugar Tech. 2013; 16 (1):1-8.
Chicago/Turabian StyleSabrina E. Martiniano; Rafael Philippini; Anuj K. Chandel; Carlos A. Rosa; Fernando C. Pagnocca; Silvio Silvério Da Silva. 2013. "Evaluation of Rice Bran Extract as a Nitrogen Source for Improved Hemicellulosic Ethanol Production from Sugarcane Bagasse by New Xylose-Fermenting Yeast Strains Isolated from Brazilian Forests." Sugar Tech 16, no. 1: 1-8.