This page has only limited features, please log in for full access.

Unclaimed
Aglaia Pappa
Department of Molecular Biology and Genetics, Democritus University of Thrace, University Campus-Dragana, 68100 Alexandroupolis, Greece

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 30 May 2021 in Antioxidants
Reads 0
Downloads 0

Lippia citriodora is a flowering plant cultivated for its lemon-scented leaves and used in folk medicine for the preparation of tea for the alleviation of symptoms of gastrointestinal disorders, cold, and asthma. The oil extracted from the plant leaves was shown to possess antioxidant potential and to exert antiproliferative activity against breast cancer. The aim of this study was to further investigate potential antitumor effects of L. citriodora oil (LCO) on breast cancer. The in vitro antiproliferative activity of LCO was examined against murine DA3 breast cancer cells by the sulforhodamine B assay. We further explored the LCO’s pro-apoptotic potential with the Annexin-PI method. The LCO’s anti-migratory effect was assessed by the wound-healing assay. LCO was found to inhibit the growth of DA3 cells in vitro, attenuate their migration, and induce apoptosis. Finally, oral administration of LCO for 14 days in mice inhibited by 55% the size of developing tumors in the DA3 murine tumor model. Noteworthy, in the tumor tissue of LCO-treated mice the apoptotic marker cleaved caspase-3 was elevated, while a reduced protein expression of survivin was observed. These results indicate that LCO, as a source of bioactive compounds, has a very interesting nutraceutical potential.

ACS Style

Katerina Spyridopoulou; Tamara Aravidou; Evangeli Lampri; Eleni Effraimidou; Aglaia Pappa; Katerina Chlichlia. Antitumor Potential of Lippia citriodora Essential Oil in Breast Tumor-Bearing Mice. Antioxidants 2021, 10, 875 .

AMA Style

Katerina Spyridopoulou, Tamara Aravidou, Evangeli Lampri, Eleni Effraimidou, Aglaia Pappa, Katerina Chlichlia. Antitumor Potential of Lippia citriodora Essential Oil in Breast Tumor-Bearing Mice. Antioxidants. 2021; 10 (6):875.

Chicago/Turabian Style

Katerina Spyridopoulou; Tamara Aravidou; Evangeli Lampri; Eleni Effraimidou; Aglaia Pappa; Katerina Chlichlia. 2021. "Antitumor Potential of Lippia citriodora Essential Oil in Breast Tumor-Bearing Mice." Antioxidants 10, no. 6: 875.

Journal article
Published: 27 February 2021 in International Journal of Molecular Sciences
Reads 0
Downloads 0

Surface active agents (SAAs), currently used in modern industry, are synthetic chemicals produced from non-renewable sources, with potential toxic impacts on humans and the environment. Thus, there is an increased interest for the identification and utilization of natural derived SAAs. As such, the marine environment is considered a promising source of biosurfactants with low toxicity, environmental compatibility, and biodegradation compared to their synthetic counterparts. MARISURF is a Horizon 2020 EU-funded project aiming to identify and functionally characterize SAAs, derived from a unique marine bacterial collection, towards commercial exploitation. Specifically, rhamnolipids produced by Marinobacter MCTG107b and Pseudomonas MCTG214(3b1) strains were previously identified and characterized while currently their toxicity profile was assessed by utilizing well-established methodologies. Our results showed a lack of cytotoxicity in in vitro models of human skin and liver as indicated by alamar blue and propidium iodide assays. Additionally, the use of the single gel electrophoresis assay, under oxidative stress conditions, revealed absence of any significant mutagenic/anti-mutagenic potential. Finally, both 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) cell-free assays, revealed no significant anti-oxidant capacity for neither of the tested compounds. Consequently, the absence of significant cytotoxicity and/or mutagenicity justifies their commercial exploitation and potential development into industrial end-user applications as natural and environmentally friendly biosurfactants.

ACS Style

Georgia-Persephoni Voulgaridou; Theodora Mantso; Ioannis Anestopoulos; Ariel Klavaris; Christina Katzastra; Despoina-Eugenia Kiousi; Marini Mantela; Alex Galanis; Konstantinos Gardikis; Ibrahim Banat; Tony Gutierrez; Karina Sałek; Stephen Euston; Mihalis Panayiotidis; Aglaia Pappa. Toxicity Profiling of Biosurfactants Produced by Novel Marine Bacterial Strains. International Journal of Molecular Sciences 2021, 22, 2383 .

AMA Style

Georgia-Persephoni Voulgaridou, Theodora Mantso, Ioannis Anestopoulos, Ariel Klavaris, Christina Katzastra, Despoina-Eugenia Kiousi, Marini Mantela, Alex Galanis, Konstantinos Gardikis, Ibrahim Banat, Tony Gutierrez, Karina Sałek, Stephen Euston, Mihalis Panayiotidis, Aglaia Pappa. Toxicity Profiling of Biosurfactants Produced by Novel Marine Bacterial Strains. International Journal of Molecular Sciences. 2021; 22 (5):2383.

Chicago/Turabian Style

Georgia-Persephoni Voulgaridou; Theodora Mantso; Ioannis Anestopoulos; Ariel Klavaris; Christina Katzastra; Despoina-Eugenia Kiousi; Marini Mantela; Alex Galanis; Konstantinos Gardikis; Ibrahim Banat; Tony Gutierrez; Karina Sałek; Stephen Euston; Mihalis Panayiotidis; Aglaia Pappa. 2021. "Toxicity Profiling of Biosurfactants Produced by Novel Marine Bacterial Strains." International Journal of Molecular Sciences 22, no. 5: 2383.

Journal article
Published: 23 February 2021 in Plants
Reads 0
Downloads 0

Propolis is a resinous substance produced by bees that exhibits antimicrobial, immunostimulatory and antioxidant activity. Its use is common in functional foods, cosmetics and traditional medicine despite the fact that it demonstrates low extraction yields and inconsistency in non-toxic solvents. In this work, a new encapsulation and delivery system consisting of liposomes and cyclodextrins incorporating propolis polyphenols has been developed and characterized. The antioxidant, antimutagenic and antiaging properties of the system under normal and UVB-induced oxidative stress conditions were investigated in cultured skin cells and/or reconstituted skin model. Furthermore, the transcript accumulation for an array of genes involved in many skin-related processes was studied. The system exhibits significant polyphenol encapsulation efficiency, physicochemical stability as well as controlled release rate in appropriate conditions. The delivery system can retain the anti-mutagenic, anti-oxidative and anti-ageing effects of propolis polyphenols to levels similar and comparable to those of propolis methanolic extracts, making the system ideal for applications where non-toxic solvents are required and controlled release of the polyphenol content is desired.

ACS Style

Eleni Spanidi; Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Sophia Letsiou; Nektarios Aligiannis; Ilias Tsochantaridis; Spyridon Kynigopoulos; Maria Lambropoulou; Ioannis Mourtzinos; Aglaia Pappa; Konstantinos Gardikis. A New Controlled Release System for Propolis Polyphenols and Its Biochemical Activity for Skin Applications. Plants 2021, 10, 420 .

AMA Style

Eleni Spanidi, Athanasios Karapetsas, Georgia-Persephoni Voulgaridou, Sophia Letsiou, Nektarios Aligiannis, Ilias Tsochantaridis, Spyridon Kynigopoulos, Maria Lambropoulou, Ioannis Mourtzinos, Aglaia Pappa, Konstantinos Gardikis. A New Controlled Release System for Propolis Polyphenols and Its Biochemical Activity for Skin Applications. Plants. 2021; 10 (2):420.

Chicago/Turabian Style

Eleni Spanidi; Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Sophia Letsiou; Nektarios Aligiannis; Ilias Tsochantaridis; Spyridon Kynigopoulos; Maria Lambropoulou; Ioannis Mourtzinos; Aglaia Pappa; Konstantinos Gardikis. 2021. "A New Controlled Release System for Propolis Polyphenols and Its Biochemical Activity for Skin Applications." Plants 10, no. 2: 420.

Journal article
Published: 13 February 2021 in Antioxidants
Reads 0
Downloads 0

Malignant melanoma is one of the most deadly types of solid cancers, a property mainly attributed to its highly aggressive metastatic form. On the other hand, different classes of isothiocyanates, a class of phytochemicals, present in cruciferous vegetables have been characterized by considerable anti-cancer activity in both in vitro and in vivo experimental models. In the current study, we investigated the anti-cancer response of five isothiocyanates in an in vitro model of melanoma consisting of non-metastatic (A375, B16F-10) and metastatic (VMM1, Hs294T) malignant melanoma as well as non-melanoma epidermoid carcinoma (A431) and non-tumorigenic melanocyte-neighboring keratinocyte (HaCaT) cells. Our aim was to compare different endpoints of cytotoxicity (e.g., reactive oxygen species, intracellular glutathione content, cell cycle growth arrest, apoptosis and necrosis) descriptive of an anti-cancer response between non-metastatic and metastatic melanoma as well as non-melanoma epidermoid carcinoma and non-tumorigenic cells. Our results showed that exposure to isothiocyanates induced an increase in intracellular reactive oxygen species and glutathione contents between non-metastatic and metastatic melanoma cells. The distribution of cell cycle phases followed a similar pattern in a manner where non-metastatic and metastatic melanoma cells appeared to be growth arrested at the G2/M phase while elevated levels of metastatic melanoma cells were shown to be at sub G1 phase, an indicator of necrotic cell death. Finally, metastatic melanoma cells were more sensitive apoptosis and/or necrosis as higher levels were observed compared to non-melanoma epidermoid carcinoma and non-tumorigenic cells. In general, non-melanoma epidermoid carcinoma and non-tumorigenic cells were more resistant under any experimental exposure condition. Overall, our study provides further evidence for the potential development of isothiocyanates as promising anti-cancer agents against non-metastatic and metastatic melanoma cells, a property specific for these cells and not shared by non-melanoma epidermoid carcinoma or non-tumorigenic melanocyte cells.

ACS Style

Melina Mitsiogianni; Sotiris Kyriakou; Ioannis Anestopoulos; Dimitrios Trafalis; Maria Deligiorgi; Rodrigo Franco; Aglaia Pappa; Mihalis Panayiotidis. An Evaluation of the Anti-Carcinogenic Response of Major Isothiocyanates in Non-Metastatic and Metastatic Melanoma Cells. Antioxidants 2021, 10, 284 .

AMA Style

Melina Mitsiogianni, Sotiris Kyriakou, Ioannis Anestopoulos, Dimitrios Trafalis, Maria Deligiorgi, Rodrigo Franco, Aglaia Pappa, Mihalis Panayiotidis. An Evaluation of the Anti-Carcinogenic Response of Major Isothiocyanates in Non-Metastatic and Metastatic Melanoma Cells. Antioxidants. 2021; 10 (2):284.

Chicago/Turabian Style

Melina Mitsiogianni; Sotiris Kyriakou; Ioannis Anestopoulos; Dimitrios Trafalis; Maria Deligiorgi; Rodrigo Franco; Aglaia Pappa; Mihalis Panayiotidis. 2021. "An Evaluation of the Anti-Carcinogenic Response of Major Isothiocyanates in Non-Metastatic and Metastatic Melanoma Cells." Antioxidants 10, no. 2: 284.

Journal article
Published: 18 January 2021 in Antioxidants
Reads 0
Downloads 0

The antioxidant, cytoprotective, and wound-healing potential of the essential oil from the resin of Pistacia lentiscus var. chia (mastic oil) was evaluated, along with that of its major components, myrcene and α-pinene. Antioxidant potential was monitored as: (i) direct antioxidant activity as assessed by 2,2-di-phenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and ABTS assays; (ii) DNA damage protection activity; and (iii) cytoprotective activity as assessed via induction of transcription of genes related to the antioxidant response in human keratinocyte cells (HaCaT). The cytoprotective potential of the test substances was further evaluated against ultraviolet radiation B (UVB)- or H2O2-induced oxidative damage, whereas their regenerative capability was accessed by monitoring the wound closure rate in HaCaT. Μastic oil and major components did not show significant direct antioxidant activity, however they increased the mRNA levels of antioxidant response genes, suggesting indirect antioxidant activity. Treatment of HaCaT with the test substances before and after UVB irradiation resulted in increased cell viability in the cases of pre-treatment with mastic oil or post-treatment with myrcene. Increased cytoprotection was also observed in the case of cell treatment with mastic oil or its major components prior to H2O2 exposure. Finally, mastic oil and myrcene demonstrated a favorable dose-dependent effect for cell migration and wound closure. Collectively, mastic essential oil may exert its promising cytoprotective properties through indirect antioxidant mechanisms.

ACS Style

Vasileios Xanthis; Eleni Fitsiou; Georgia-Persephoni Voulgaridou; Athanasios Bogadakis; Katerina Chlichlia; Alex Galanis; Aglaia Pappa. Antioxidant and Cytoprotective Potential of the Essential Oil Pistacia lentiscus var. chia and Its Major Components Myrcene and α-Pinene. Antioxidants 2021, 10, 127 .

AMA Style

Vasileios Xanthis, Eleni Fitsiou, Georgia-Persephoni Voulgaridou, Athanasios Bogadakis, Katerina Chlichlia, Alex Galanis, Aglaia Pappa. Antioxidant and Cytoprotective Potential of the Essential Oil Pistacia lentiscus var. chia and Its Major Components Myrcene and α-Pinene. Antioxidants. 2021; 10 (1):127.

Chicago/Turabian Style

Vasileios Xanthis; Eleni Fitsiou; Georgia-Persephoni Voulgaridou; Athanasios Bogadakis; Katerina Chlichlia; Alex Galanis; Aglaia Pappa. 2021. "Antioxidant and Cytoprotective Potential of the Essential Oil Pistacia lentiscus var. chia and Its Major Components Myrcene and α-Pinene." Antioxidants 10, no. 1: 127.

Journal article
Published: 06 January 2021 in Biomedicines
Reads 0
Downloads 0

Aldehyde dehydrogenases (ALDHs) are NAD(P)+-dependent enzymes that catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding carboxylic acids. ALDHs participate in a variety of cellular mechanisms, such as metabolism, cell proliferation and apoptosis, as well as differentiation and stemness. Over the last few years, ALDHs have emerged as cancer stem cell markers in a wide spectrum of solid tumors and hematological malignancies. In this study, the pathophysiological role of ALDH1B1 in human colorectal adenocarcinoma was investigated. Human colon cancer HT29 cells were stably transfected either with human green fluorescent protein (GFP)-tagged ALDH1B1 or with an empty lentiviral expression vector. The overexpression of ALDH1B1 was correlated with altered cell morphology, decreased proliferation rate and reduced clonogenic efficiency. Additionally, ALDH1B1 triggered a G2/M arrest at 24 h post-cell synchronization, probably through p53 and p21 upregulation. Furthermore, ALDH1B1-overexpressing HT29 cells exhibited enhanced resistance against doxorubicin, fluorouracil (5-FU) and etoposide. Finally, ALDH1B1 induced increased migratory potential and displayed epithelial–mesenchymal transition (EMT) through the upregulation of ZEB1 and vimentin and the consequent downregulation of E-cadherin. Taken together, ALDH1B1 confers alterations in the cell morphology, cell cycle progression and gene expression, accompanied by significant changes in the chemosensitivity and migratory potential of HT29 cells, underlying its potential significance in cancer progression.

ACS Style

Ilias Tsochantaridis; Angelos Roupas; Georgia-Persephoni Voulgaridou; Alexandra Giatromanolaki; Michael I. Koukourakis; Mihalis I. Panayiotidis; Aglaia Pappa. Aldehyde Dehydrogenase 1B1 Is Associated with Altered Cell Morphology, Proliferation, Migration and Chemosensitivity in Human Colorectal Adenocarcinoma Cells. Biomedicines 2021, 9, 44 .

AMA Style

Ilias Tsochantaridis, Angelos Roupas, Georgia-Persephoni Voulgaridou, Alexandra Giatromanolaki, Michael I. Koukourakis, Mihalis I. Panayiotidis, Aglaia Pappa. Aldehyde Dehydrogenase 1B1 Is Associated with Altered Cell Morphology, Proliferation, Migration and Chemosensitivity in Human Colorectal Adenocarcinoma Cells. Biomedicines. 2021; 9 (1):44.

Chicago/Turabian Style

Ilias Tsochantaridis; Angelos Roupas; Georgia-Persephoni Voulgaridou; Alexandra Giatromanolaki; Michael I. Koukourakis; Mihalis I. Panayiotidis; Aglaia Pappa. 2021. "Aldehyde Dehydrogenase 1B1 Is Associated with Altered Cell Morphology, Proliferation, Migration and Chemosensitivity in Human Colorectal Adenocarcinoma Cells." Biomedicines 9, no. 1: 44.

Review
Published: 21 July 2020 in Pharmaceutics
Reads 0
Downloads 0

Surface active agents (SAAs) are molecules with the capacity to adsorb to solid surfaces and/or fluid interfaces, a property that allows them to act as multifunctional ingredients (e.g., wetting and dispersion agents, emulsifiers, foaming and anti-foaming agents, lubricants, etc.) in a widerange of the consumer products of various industrial sectors (e.g., pharmaceuticals, cosmetics, personal care, detergents, food, etc.). Given their widespread utilization, there is a continuously growing interest to explore their role in consumer products (relevant to promoting human health) and how such information can be utilized in order to synthesize better chemical derivatives. In this review article, weaimed to provide updated information on synthetic and biological (biosurfactants) SAAs and their health-promoting properties (e.g., anti-microbial, anti-oxidant, anti-viral, anti-inflammatory, anti-cancer and anti-aging) in an attempt to better define some of the underlying mechanism(s) by which they exert such properties.

ACS Style

Ioannis Anestopoulos; Despoina Eugenia Kiousi; Ariel Klavaris; Alex Galanis; Karina Salek; Stephen R. Euston; Aglaia Pappa; Mihalis I. Panayiotidis. Surface Active Agents and Their Health-Promoting Properties: Molecules of Multifunctional Significance. Pharmaceutics 2020, 12, 688 .

AMA Style

Ioannis Anestopoulos, Despoina Eugenia Kiousi, Ariel Klavaris, Alex Galanis, Karina Salek, Stephen R. Euston, Aglaia Pappa, Mihalis I. Panayiotidis. Surface Active Agents and Their Health-Promoting Properties: Molecules of Multifunctional Significance. Pharmaceutics. 2020; 12 (7):688.

Chicago/Turabian Style

Ioannis Anestopoulos; Despoina Eugenia Kiousi; Ariel Klavaris; Alex Galanis; Karina Salek; Stephen R. Euston; Aglaia Pappa; Mihalis I. Panayiotidis. 2020. "Surface Active Agents and Their Health-Promoting Properties: Molecules of Multifunctional Significance." Pharmaceutics 12, no. 7: 688.

Journal article
Published: 01 July 2020 in Foods
Reads 0
Downloads 0

The aim of the present study was to investigate the antimicrobial potential of Sideritis raeseri subps. raeseri essential oil (EO) against common food spoilage and pathogenic microorganisms and evaluate its antioxidant and antiproliferative activity. The EO was isolated by steam distillation and analyzed by GC/MS. The main constituents identified were geranyl-p-cymene (25.08%), geranyl-γ-terpinene (15.17%), and geranyl-linalool (14.04%). Initially, its activity against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Listeria monocytogenes, Salmonella Enteritidis, Salmonella Typhimurium, Pseudomonas fragi, Saccharomyces cerevisiae, and Aspergillus niger was screened by the disk diffusion method. Subsequently, minimum inhibitory concentration (MIC), non-inhibitory concentration (NIC), and minimum lethal concentration (MLC) values were determined. Growth inhibition of all microorganisms tested was documented, although it was significantly lower compared to gentamycin, ciproxin, and voriconazole, which were used as positive controls. In a next step, its direct antioxidant properties were examined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, and the IC50 values were determined. The potential cytoprotective activity of the oil against H2O2–induced oxidative stress and DNA damage was studied in human immortalized keratinocyte (HaCaT) cells using the comet assay. Finally, the antiproliferative activity of the oil was evaluated against a panel of cancer cell lines including A375, Caco2, PC3, and DU145 and the non-cancerous HaCaT cell line using the sulforhodamine B (SRB) assay, and the EC50 values were determined. The oil demonstrated weak radical scavenging activity, noteworthy cytoprotective activity against H2O2–induced oxidative stress and DNA damage in HaCaT cells, and antiproliferative activity against all cell lines tested, being more sensitive against the in vitro model of skin melanoma.

ACS Style

Gregoria Mitropoulou; Marianthi Sidira; Myria Skitsa; Ilias Tsochantaridis; Aglaia Pappa; Christos Dimtsoudis; Charalampos Proestos; Yiannis Kourkoutas. Assessment of the Antimicrobial, Antioxidant, and Antiproliferative Potential of Sideritisraeseri subps. raeseri Essential Oil. Foods 2020, 9, 860 .

AMA Style

Gregoria Mitropoulou, Marianthi Sidira, Myria Skitsa, Ilias Tsochantaridis, Aglaia Pappa, Christos Dimtsoudis, Charalampos Proestos, Yiannis Kourkoutas. Assessment of the Antimicrobial, Antioxidant, and Antiproliferative Potential of Sideritisraeseri subps. raeseri Essential Oil. Foods. 2020; 9 (7):860.

Chicago/Turabian Style

Gregoria Mitropoulou; Marianthi Sidira; Myria Skitsa; Ilias Tsochantaridis; Aglaia Pappa; Christos Dimtsoudis; Charalampos Proestos; Yiannis Kourkoutas. 2020. "Assessment of the Antimicrobial, Antioxidant, and Antiproliferative Potential of Sideritisraeseri subps. raeseri Essential Oil." Foods 9, no. 7: 860.

Journal article
Published: 30 June 2020 in Antioxidants
Reads 0
Downloads 0

In the present study, we aimed to examine the antioxidant, antiaging and photoprotective properties of Greek honey samples of various botanical and geographical origin. Ethyl-acetate extracts were used and the and the total phenolic/flavonoid content and antioxidant capacity were evaluated. Honey extracts were then studied for their cytoprotective properties against UVB-induced photodamage using human immortalized keratinocytes (HaCaT) and/or reconstituted human skin tissue models. Specifically, the cytotoxicity, oxidative status, DNA damage and gene expression levels of specific matrix metalloproteinases (MMPs) were examined. Overall, the treatment of HaCaT cells with honey extracts resulted in lower levels of DNA strand breaks and attenuated the decrease in cell viability following UVB exposure. Additionally, honey extracts significantly decreased the total protein carbonyl content of the irradiated cells, however, they had no significant effect on their total antioxidant status. Finally, the extracts alleviated the UVB-induced up-regulation of MMPs-3, -7 and -9 in a model of reconstituted skin tissue. In conclusion, honey extracts exhibited significant photoprotective and antiaging properties under UVB exposure conditions and thus could be further exploited as promising agents for developing novel and naturally-based, antiaging cosmeceutical products.

ACS Style

Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Dimitra Iliadi; Ilias Tsochantaridis; Panagiota Michail; Spyridon Kynigopoulos; Maria Lambropoulou; Maria-Ioanna Stavropoulou; Konstantina Stathopoulou; Sofia Karabournioti; Nektarios Aligiannis; Konstantinos Gardikis; Alex Galanis; Mihalis I. Panayiotidis; Aglaia Pappa. Honey Extracts Exhibit Cytoprotective Properties against UVB-Induced Photodamage in Human Experimental Skin Models. Antioxidants 2020, 9, 566 .

AMA Style

Athanasios Karapetsas, Georgia-Persephoni Voulgaridou, Dimitra Iliadi, Ilias Tsochantaridis, Panagiota Michail, Spyridon Kynigopoulos, Maria Lambropoulou, Maria-Ioanna Stavropoulou, Konstantina Stathopoulou, Sofia Karabournioti, Nektarios Aligiannis, Konstantinos Gardikis, Alex Galanis, Mihalis I. Panayiotidis, Aglaia Pappa. Honey Extracts Exhibit Cytoprotective Properties against UVB-Induced Photodamage in Human Experimental Skin Models. Antioxidants. 2020; 9 (7):566.

Chicago/Turabian Style

Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Dimitra Iliadi; Ilias Tsochantaridis; Panagiota Michail; Spyridon Kynigopoulos; Maria Lambropoulou; Maria-Ioanna Stavropoulou; Konstantina Stathopoulou; Sofia Karabournioti; Nektarios Aligiannis; Konstantinos Gardikis; Alex Galanis; Mihalis I. Panayiotidis; Aglaia Pappa. 2020. "Honey Extracts Exhibit Cytoprotective Properties against UVB-Induced Photodamage in Human Experimental Skin Models." Antioxidants 9, no. 7: 566.

Review
Published: 09 June 2020 in Biomolecules
Reads 0
Downloads 0

Surface active agents are characterized for their capacity to adsorb to fluid and solid-water interfaces. They can be classified as surfactants and emulsifiers based on their molecular weight (MW) and properties. Over the years, the chemical surfactant industry has been rapidly increasing to meet consumer demands. Consequently, such a boost has led to the search for more sustainable and biodegradable alternatives, as chemical surfactants are non-biodegradable, thus causing an adverse effect on the environment. To these ends, many microbial and/or marine-derived molecules have been shown to possess various biological properties that could allow manufacturers to make additional health-promoting claims for their products. Our aim, in this review article, is to provide up to date information of critical health-promoting properties of these molecules and their use in blue-based biotechnology (i.e., biotechnology using aquatic organisms) with a focus on food, cosmetic and pharmaceutical/biomedical applications.

ACS Style

Ioannis Anestopoulos; Despina-Evgenia Kiousi; Ariel Klavaris; Monica Maijo; Annabel Serpico; Alba Suarez; Guiomar Sanchez; Karina Salek; Stylliani A. Chasapi; Aikaterini A. Zompra; Alex Galanis; Georgios A. Spyroulias; Lourdes Gombau; Stephen R. Euston; Aglaia Pappa; Mihalis I. Panayiotidis. Marine-Derived Surface Active Agents: Health-Promoting Properties and Blue Biotechnology-Based Applications. Biomolecules 2020, 10, 885 .

AMA Style

Ioannis Anestopoulos, Despina-Evgenia Kiousi, Ariel Klavaris, Monica Maijo, Annabel Serpico, Alba Suarez, Guiomar Sanchez, Karina Salek, Stylliani A. Chasapi, Aikaterini A. Zompra, Alex Galanis, Georgios A. Spyroulias, Lourdes Gombau, Stephen R. Euston, Aglaia Pappa, Mihalis I. Panayiotidis. Marine-Derived Surface Active Agents: Health-Promoting Properties and Blue Biotechnology-Based Applications. Biomolecules. 2020; 10 (6):885.

Chicago/Turabian Style

Ioannis Anestopoulos; Despina-Evgenia Kiousi; Ariel Klavaris; Monica Maijo; Annabel Serpico; Alba Suarez; Guiomar Sanchez; Karina Salek; Stylliani A. Chasapi; Aikaterini A. Zompra; Alex Galanis; Georgios A. Spyroulias; Lourdes Gombau; Stephen R. Euston; Aglaia Pappa; Mihalis I. Panayiotidis. 2020. "Marine-Derived Surface Active Agents: Health-Promoting Properties and Blue Biotechnology-Based Applications." Biomolecules 10, no. 6: 885.

Original contribution
Published: 25 March 2020 in European Journal of Nutrition
Reads 0
Downloads 0

Growing evidence supports that isothiocyanates exert a wide range of bioactivities amongst of which is their capacity to interact with the epigenetic machinery in various cancers including melanoma. Our aim was to characterise the effect of sulforaphane and iberin on histone acetylation and methylation as a potential anti-melanoma strategy. We have utilised an in vitro model of malignant melanoma [consisting of human (A375, Hs294T, VMM1) and murine (B16F-10) melanoma cell lines as well as a non-melanoma (A431) and a non-tumorigenic immortalised keratinocyte (HaCaT) cell line] exposed to sulforaphane or iberin. Cell viability was evaluated by the Alamar blue assay whilst total histone deacetylases and acetyltransferases activities were determined by the Epigenase HDAC Activity/Inhibition and EpiQuik HAT Activity/Inhibition assay kits, respectively. The expression levels of specific histone deacetylases and acetyltransferases together with those of lysine acetylation and methylation marks were obtained by western immunoblotting. Overall, both sulforaphane and iberin were able to (1) reduce cell viability, (2) decrease total histone deacetylase activity and (3) modulate the expression levels of various histone deacetylases as well as acetyl and methyl transferases thus modulating the acetylation and methylation status of specific lysine residues on histones 3 and 4 in malignant melanoma cells. Our findings highlight novel insights as to how sulforaphane and iberin differentially regulate the epigenetic response in ways compatible with their anticancer action in malignant melanoma.

ACS Style

Melina Mitsiogianni; Dimitrios T. Trafalis; Rodrigo Franco; Vasilis Zoumpourlis; Aglaia Pappa; Mihalis I. Panayiotidis. Sulforaphane and iberin are potent epigenetic modulators of histone acetylation and methylation in malignant melanoma. European Journal of Nutrition 2020, 60, 147 -158.

AMA Style

Melina Mitsiogianni, Dimitrios T. Trafalis, Rodrigo Franco, Vasilis Zoumpourlis, Aglaia Pappa, Mihalis I. Panayiotidis. Sulforaphane and iberin are potent epigenetic modulators of histone acetylation and methylation in malignant melanoma. European Journal of Nutrition. 2020; 60 (1):147-158.

Chicago/Turabian Style

Melina Mitsiogianni; Dimitrios T. Trafalis; Rodrigo Franco; Vasilis Zoumpourlis; Aglaia Pappa; Mihalis I. Panayiotidis. 2020. "Sulforaphane and iberin are potent epigenetic modulators of histone acetylation and methylation in malignant melanoma." European Journal of Nutrition 60, no. 1: 147-158.

Journal article
Published: 05 February 2020 in Cancers
Reads 0
Downloads 0

The role of dietary probiotic strains on host anti-cancer immune responses against experimental colon carcinoma was investigated. We have previously shown that Lactobacillus casei administration led to tumor growth suppression in an experimental colon cancer model. Here, we investigated the underlying immune mechanisms involved in this tumor-growth inhibitory effect. BALB/c mice received daily live lactobacilli per os prior to the establishment of a syngeneic subcutaneous CT26 tumor. Tumor volume, cytokine production, T cell differentiation and migration, as well as tumor cell apoptosis were examined to outline potential immunomodulatory effects following L. casei oral intake. Probiotic administration in mice resulted in a significant increase in interferon gamma (IFN-γ), Granzyme B and chemokine production in the tumor tissue as well as enhanced CD8+ T cell infiltration, accompanied by a suppression of tumor growth. Cytotoxic activity against cancer cells was enhanced in probiotic-fed compared to control mice, as evidenced by the elevation of apoptotic markers, such as cleaved caspase 3 and poly (ADP-ribose) polymerase 1 (PARP1), in tumor tissue. Oral administration of Lactobacillus casei induced potent Th1 immune responses and cytotoxic T cell infiltration in the tumor tissue of tumor-bearing mice, resulting in tumor growth inhibition. Thus, the microorganism may hold promise as a novel dietary immunoadjuvant in raising protective anti-cancer immune responses.

ACS Style

Georgios Aindelis; Angeliki Tiptiri-Kourpeti; Evangeli Lampri; Katerina Spyridopoulou; Eleftheria Lamprianidou; Ioannis Kotsianidis; Petros Ypsilantis; Aglaia Pappa; Katerina Chlichlia. Immune Responses Raised in an Experimental Colon Carcinoma Model Following Oral Administration of Lactobacillus casei. Cancers 2020, 12, 368 .

AMA Style

Georgios Aindelis, Angeliki Tiptiri-Kourpeti, Evangeli Lampri, Katerina Spyridopoulou, Eleftheria Lamprianidou, Ioannis Kotsianidis, Petros Ypsilantis, Aglaia Pappa, Katerina Chlichlia. Immune Responses Raised in an Experimental Colon Carcinoma Model Following Oral Administration of Lactobacillus casei. Cancers. 2020; 12 (2):368.

Chicago/Turabian Style

Georgios Aindelis; Angeliki Tiptiri-Kourpeti; Evangeli Lampri; Katerina Spyridopoulou; Eleftheria Lamprianidou; Ioannis Kotsianidis; Petros Ypsilantis; Aglaia Pappa; Katerina Chlichlia. 2020. "Immune Responses Raised in an Experimental Colon Carcinoma Model Following Oral Administration of Lactobacillus casei." Cancers 12, no. 2: 368.

Journal article
Published: 30 January 2020 in Free Radical Biology and Medicine
Reads 0
Downloads 0

Aldehyde dehydrogenase 3A1 is constitutively expressed in a taxon-specific manner in the cornea, where, due to its high abundance, it has been characterized as a corneal crystallin. ALDH3A1 has been proposed to be a multifaceted protein that protects cellular homeostasis through several modes of action. The present study examines the mechanisms by which ALDH3A1 exerts its cytoprotective role under conditions of oxidative stress. To this end, we have utilized an isogenic HCE-2 (human corneal epithelium) cell line pair differing in the expression of ALDH3A1. Single cell gel electrophoresis assay and H2DCFDA analysis revealed that the expression of ALDH3A1 protected HCE-2 cells from H2O2-, tert-butyl peroxide- and etoposide-induced oxidative and genotoxic effects. Furthermore, comparative qPCR analysis revealed that a panel of cell cycle (Cyclins B1, B2, D, E), apoptosis (p53, BAX, BCL-2, BCL-XL) and DNA damage response (DNA-PK, NBS1) genes were up-regulated in the ALDH3A1 expressing HCE-2 cells. Moreover, the expression profile of a variety of DNA damage signaling (DDS)-related genes, was investigated (under normal and oxidative stress conditions) by utilizing the RT2 profiler™ PCR array in both isogenic HCE-2 cell lines. Our results demonstrated that several genes associated with ATM/ATR signaling, cell cycle regulation, apoptosis and DNA damage repair were differentially expressed under all conditions tested. In conclusion, this study suggests that ALDH3A1 significantly contributes to the antioxidant defense of corneal homeostasis by maintaining DNA integrity possibly through altering the expression of specific DDS-related genes. Further studies will shed light on the precise role(s) of this multifunctional protein.

ACS Style

Georgia-Persephoni Voulgaridou; Ilias Tsochantaridis; Christos Tolkas; Rodrigo Franco; Alexandra Giatromanolaki; Mihalis I. Panayiotidis; Aglaia Pappa. Aldehyde dehydrogenase 3A1 confers oxidative stress resistance accompanied by altered DNA damage response in human corneal epithelial cells. Free Radical Biology and Medicine 2020, 150, 66 -74.

AMA Style

Georgia-Persephoni Voulgaridou, Ilias Tsochantaridis, Christos Tolkas, Rodrigo Franco, Alexandra Giatromanolaki, Mihalis I. Panayiotidis, Aglaia Pappa. Aldehyde dehydrogenase 3A1 confers oxidative stress resistance accompanied by altered DNA damage response in human corneal epithelial cells. Free Radical Biology and Medicine. 2020; 150 ():66-74.

Chicago/Turabian Style

Georgia-Persephoni Voulgaridou; Ilias Tsochantaridis; Christos Tolkas; Rodrigo Franco; Alexandra Giatromanolaki; Mihalis I. Panayiotidis; Aglaia Pappa. 2020. "Aldehyde dehydrogenase 3A1 confers oxidative stress resistance accompanied by altered DNA damage response in human corneal epithelial cells." Free Radical Biology and Medicine 150, no. : 66-74.

Review article
Published: 20 January 2020 in Mitochondrion
Reads 0
Downloads 0

Autophagy is a ubiquitous homeostatic mechanism for the degradation or turnover of cellular components. Degradation of mitochondria via autophagy (mitophagy) is involved in a number of physiological processes including cellular homeostasis, differentiation and aging. Upon stress or injury, mitophagy prevents the accumulation of damaged mitochondria and the increased steady state levels of reactive oxygen species leading to oxidative stress and cell death. A number of human diseases, particularly neurodegenerative disorders, have been linked to the dysregulation of mitophagy. In this mini-review, we aimed to review the molecular mechanisms involved in the regulation of mitophagy and their relationship with redox signaling and oxidative stress.

ACS Style

Carla Garza-Lombo; Aglaia Pappa; Mihalis I. Panayiotidis; Rodrigo Franco. Redox homeostasis, oxidative stress and mitophagy. Mitochondrion 2020, 51, 105 -117.

AMA Style

Carla Garza-Lombo, Aglaia Pappa, Mihalis I. Panayiotidis, Rodrigo Franco. Redox homeostasis, oxidative stress and mitophagy. Mitochondrion. 2020; 51 ():105-117.

Chicago/Turabian Style

Carla Garza-Lombo; Aglaia Pappa; Mihalis I. Panayiotidis; Rodrigo Franco. 2020. "Redox homeostasis, oxidative stress and mitophagy." Mitochondrion 51, no. : 105-117.

Research article
Published: 13 January 2020 in Chemical Research in Toxicology
Reads 0
Downloads 0

Metal-derived nanoparticles (Mt-NPs) are increasingly used in cosmetology due to their ultraviolet shielding (titanium dioxide [TiO2]), antioxidant (cerium dioxide [CeO2]), and biocidal (silver [Ag]) properties. In the absence of overt toxicity (i.e. cell death), Mt-NPs are considered safe for cosmetic applications. However, there is little understanding about the mechanisms involved in the survival of keratinocytes exposed to subtoxic levels of Mt-NPs. Human keratinocytes (HaCaT) were exposed subacutely to subtoxic concentrations (≤30 μg/ml, 48-72 h) of rutile (r) TiO2 (cylindrical), CeO2 (cubic) and Ag (spherical) with a core / hydrodynamic size of <50 / 98% purity. Mt-NP uptake was indirectly quantified by changes in the light side scatter (SSC), where the kinetics (time/dose-response) suggested that the three types of Mt-NPs were similarly uptaken by keratinocytes. rTiO2 and CeO2, but not Ag-NPs, increased autophagy, whose inhibition prompted cell death. No increase in the steady-state levels of reactive oxygen species (ROS) was induced by exposure to any of the Mt-NPs tested. Interestingly, intracellular Ag-NP aggregates observed an increased far-red autofluorescence (≥740 nm em), which has been ascribed to their binding to thiol molecules such as glutathione (GSH). Accordingly, inhibition of GSH synthesis, but not the impairment of oxidized GSH recycling, sensitized keratinocytes to Ag-NPs suggesting that GSH homeostasis, and its direct scavenging of Ag-NPs, but not ROS, is essential for keratinocyte survival upon exposure to Ag-NP. rTiO2 and Ag, but not CeO2-NPs, compromised metabolic flux (glycolysis and respiration), but ATP levels were unaltered. Finally, we also observed that exposure to Mt-NPs sensitized keratinocytes to non-UV xenobiotic exposure (arsenite and paraquat). Our results demonstrate the differential contribution of autophagy and GSH homeostasis to the survival of human keratinocytes exposed to subtoxic concentrations of Mt-NPs and highlight the increased susceptibility of keratinocytes exposed to Mt-NPs to a second xenobiotic insult.

ACS Style

Veronica Montesinos-Cruz; Jordan Rose; Aglaia Pappa; Mihalis I. Panayiotidis; Andrea De Vizcaya-Ruiz; Rodrigo Franco. Survival Mechanisms and Xenobiotic Susceptibility of Keratinocytes Exposed to Metal-Derived Nanoparticles. Chemical Research in Toxicology 2020, 33, 536 -552.

AMA Style

Veronica Montesinos-Cruz, Jordan Rose, Aglaia Pappa, Mihalis I. Panayiotidis, Andrea De Vizcaya-Ruiz, Rodrigo Franco. Survival Mechanisms and Xenobiotic Susceptibility of Keratinocytes Exposed to Metal-Derived Nanoparticles. Chemical Research in Toxicology. 2020; 33 (2):536-552.

Chicago/Turabian Style

Veronica Montesinos-Cruz; Jordan Rose; Aglaia Pappa; Mihalis I. Panayiotidis; Andrea De Vizcaya-Ruiz; Rodrigo Franco. 2020. "Survival Mechanisms and Xenobiotic Susceptibility of Keratinocytes Exposed to Metal-Derived Nanoparticles." Chemical Research in Toxicology 33, no. 2: 536-552.

Review
Published: 21 November 2019 in JBIC Journal of Biological Inorganic Chemistry
Reads 0
Downloads 0

Arsenic is a metalloid found in groundwater as a byproduct of soil/rock erosion and industrial and agricultural processes. This xenobiotic elicits its toxicity through different mechanisms, and it has been identified as a toxicant that affects virtually every organ or tissue in the body. In the central nervous system, exposure to arsenic can induce cognitive dysfunction. Furthermore, iAs has been linked to several neurological disorders, including neurodevelopmental alterations, and is considered a risk factor for neurodegenerative disorders. However, the exact mechanisms involved are still unclear. In this review, we aim to appraise the neurotoxic effects of arsenic and the molecular mechanisms involved. First, we discuss the epidemiological studies reporting on the effects of arsenic in intellectual and cognitive function during development as well as studies showing the correlation between arsenic exposure and altered cognition and mental health in adults. The neurotoxic effects of arsenic and the potential mechanisms associated with neurodegeneration are also reviewed including data from experimental models supporting epidemiological evidence of arsenic as a neurotoxicant. Next, we focused on recent literature regarding arsenic metabolism and the molecular mechanisms that begin to explain how arsenic damages the central nervous system including, oxidative stress, energy failure and mitochondrial dysfunction, epigenetics, alterations in neurotransmitter homeostasis and synaptic transmission, cell death pathways, and inflammation. Outlining the specific mechanisms by which arsenic alters the cell function is key to understand the neurotoxic effects that convey cognitive dysfunction, neurodevelopmental alterations, and neurodegenerative disorders.

ACS Style

Carla Garza-Lombo; Aglaia Pappa; Mihalis I. Panayiotidis; María E. Gonsebatt; Rodrigo Franco. Arsenic-induced neurotoxicity: a mechanistic appraisal. JBIC Journal of Biological Inorganic Chemistry 2019, 24, 1305 -1316.

AMA Style

Carla Garza-Lombo, Aglaia Pappa, Mihalis I. Panayiotidis, María E. Gonsebatt, Rodrigo Franco. Arsenic-induced neurotoxicity: a mechanistic appraisal. JBIC Journal of Biological Inorganic Chemistry. 2019; 24 (8):1305-1316.

Chicago/Turabian Style

Carla Garza-Lombo; Aglaia Pappa; Mihalis I. Panayiotidis; María E. Gonsebatt; Rodrigo Franco. 2019. "Arsenic-induced neurotoxicity: a mechanistic appraisal." JBIC Journal of Biological Inorganic Chemistry 24, no. 8: 1305-1316.

Journal article
Published: 05 September 2019 in Antioxidants
Reads 0
Downloads 0

Cornus mas L. (Cornelian cherry) is a flowering plant indigenous to Europe and parts of Asia, mostly studied for the antimicrobial activity of its juice. In this report, we investigated the composition and the in vitro antioxidant capacity of Cornus mas L. fruit juice from Greece, as well as its antiproliferative properties in vitro and in vivo. The fruits showed a high content of citric, malic, and succinic acid, in contrast to their juice, which had a low concentration of organic acids. The juice demonstrated significant antioxidant activity against the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and modest antiproliferative potential against four human cancer cells lines and one murine: mammary adenocarcinoma MCF-7, hepatocellular carcinoma HepG2 and colon adenocarcinomas Caco2, HT-29, as well as murine colon carcinoma CT26. Cell viability was reduced by 40–50% following incubation of the cells with the highest concentration of the juice. Although Cornelian cherry juice exhibited in vitro growth inhibitory effects against colon carcinoma cells, no tumor growth inhibition was observed in an in vivo experimental colon carcinoma model in mice following prophylactic oral administration of a daily dose of 100 μL juice for a period of 10 days. Thus, our findings raise interesting questions for further research on Cornus mas L. fruit juice, and in parallel, the strong antioxidant potential implies that the plant could be further explored and exploited for its protective effect against oxidative damage.

ACS Style

Angeliki Tiptiri-Kourpeti; Eleni Fitsiou; Katerina Spyridopoulou; Stavros Vasileiadis; Christos Iliopoulos; Alex Galanis; Stavroula Vekiari; Aglaia Pappa; Katerina Chlichlia; Tiptiri- Kourpeti. Evaluation of Antioxidant and Antiproliferative Properties of Cornus mas L. Fruit Juice. Antioxidants 2019, 8, 377 .

AMA Style

Angeliki Tiptiri-Kourpeti, Eleni Fitsiou, Katerina Spyridopoulou, Stavros Vasileiadis, Christos Iliopoulos, Alex Galanis, Stavroula Vekiari, Aglaia Pappa, Katerina Chlichlia, Tiptiri- Kourpeti. Evaluation of Antioxidant and Antiproliferative Properties of Cornus mas L. Fruit Juice. Antioxidants. 2019; 8 (9):377.

Chicago/Turabian Style

Angeliki Tiptiri-Kourpeti; Eleni Fitsiou; Katerina Spyridopoulou; Stavros Vasileiadis; Christos Iliopoulos; Alex Galanis; Stavroula Vekiari; Aglaia Pappa; Katerina Chlichlia; Tiptiri- Kourpeti. 2019. "Evaluation of Antioxidant and Antiproliferative Properties of Cornus mas L. Fruit Juice." Antioxidants 8, no. 9: 377.

Review
Published: 07 August 2019 in Antioxidants
Reads 0
Downloads 0

Aromatic plants have a long and significant history in the traditional medicine of many countries. Nowadays, there is an increasing interest in investigating the biological properties of aromatic plant extracts mainly due to their diversity, high availability, and low toxicity. Greece is abundant in aromatic plants, which can be attributed to the country's geographical position, the morphology of its landscape, and its numerous mountainous and insular areas. In the past 15 years, a number of aromatic plant extracts of Greek origin have been studied for their bioactivities, including their antiproliferative potential against different types of cancer. Although the pharmacological activities of specific species of Greek origin have been reviewed before, no gathered information on explicitly Greek species exist. In this review, we summarize existing data on the antiproliferative activity of extracts isolated from Greek aromatic plants and discuss their molecular mode(s) of action, where available, in order to identify promising extracts for future research and link chemical constituents responsible for their activity. We conclude that essentials oils are the most frequently studied plant extracts exhibiting high diversity in their composition and anticancer potential, but also other extracts appear to be worthy of further investigation for cancer chemoprevention.

ACS Style

Eleni Fitsiou; Aglaia Pappa. Anticancer Activity of Essential Oils and Other Extracts from Aromatic Plants Grown in Greece. Antioxidants 2019, 8, 290 .

AMA Style

Eleni Fitsiou, Aglaia Pappa. Anticancer Activity of Essential Oils and Other Extracts from Aromatic Plants Grown in Greece. Antioxidants. 2019; 8 (8):290.

Chicago/Turabian Style

Eleni Fitsiou; Aglaia Pappa. 2019. "Anticancer Activity of Essential Oils and Other Extracts from Aromatic Plants Grown in Greece." Antioxidants 8, no. 8: 290.

Journal article
Published: 18 July 2019 in Molecules
Reads 0
Downloads 0

Origanum species are plants rich in volatile oils that are mainly used for culinary purposes. In recent years, there has been a growing interest in the biological activities of their essential oils. Origanum onites L. is a plant mainly found in Greece, Turkey, and Sicily, whose oil is rich in carvacrol, a highly bioactive phytochemical. The aim of this study was to analyze the chemical composition of Origanum onites essential oil (OOEO), and investigate its potential anticancer effects in vitro and in vivo. GC/MS analysis identified carvacrol as OOEO's main constituent. In vitro antiproliferative activity was assayed with the sulforhodamine B (SRB) assay against human cancer cell lines from four tumor types. HT-29, a colorectal cancer cell line, was the most sensitive to the antiproliferative activity of OOEO. Wound-healing assay and Annexin V-PI staining were employed to investigate the antimigratory and the pro-apoptotic potential of OOEO, respectively, against human (HT-29) and murine (CT26) colon cancer cells. Notably, OOEO attenuated migration and induced apoptosis-related morphological changes in both cell lines. Prophylactic oral administration of the oil in a BALB/c experimental mouse model inhibited the growth of syngeneic CT26 colon tumors. As far as we know, this is the first report on the antitumor potential of orally administered OOEO.

ACS Style

Katerina Spyridopoulou; Eleni Fitsiou; Eleni Bouloukosta; Angeliki Tiptiri-Kourpeti; Manolis Vamvakias; Antigoni Oreopoulou; Eleni Papavassilopoulou; Aglaia Pappa; Katerina Chlichlia. Extraction, Chemical Composition, and Anticancer Potential of Origanum onites L. Essential Oil. Molecules 2019, 24, 2612 .

AMA Style

Katerina Spyridopoulou, Eleni Fitsiou, Eleni Bouloukosta, Angeliki Tiptiri-Kourpeti, Manolis Vamvakias, Antigoni Oreopoulou, Eleni Papavassilopoulou, Aglaia Pappa, Katerina Chlichlia. Extraction, Chemical Composition, and Anticancer Potential of Origanum onites L. Essential Oil. Molecules. 2019; 24 (14):2612.

Chicago/Turabian Style

Katerina Spyridopoulou; Eleni Fitsiou; Eleni Bouloukosta; Angeliki Tiptiri-Kourpeti; Manolis Vamvakias; Antigoni Oreopoulou; Eleni Papavassilopoulou; Aglaia Pappa; Katerina Chlichlia. 2019. "Extraction, Chemical Composition, and Anticancer Potential of Origanum onites L. Essential Oil." Molecules 24, no. 14: 2612.

Preclinical studies
Published: 26 June 2019 in Investigational New Drugs
Reads 0
Downloads 0

The anticancer activity of a series of novel synthesized, hydroxypyridone-based metal chelators (analogues of L-mimosine) was evaluated in an in vitro model of melanoma consisting of malignant melanoma (A375), non-melanoma epidermoid carcinoma (A431) and immortalized non-malignant keratinocyte (HaCaT) cells. More specifically, we have demonstrated that the L-enantiomer of a methylated analogue of L-mimosine (compound 22) can exert a potent anticancer effect in A375 cells when compared to either A431 or HaCaT cells. Moreover, we have demonstrated that this analogue has the ability to i) promote increased generation of reactive oxygen species (ROS), ii) activate both intrinsic and extrinsic apoptosis and iii) induce perturbations in cell cycle growth arrest. Our data highlights the potential of compound 22 to act as a promising therapeutic agent against an in vitro model of human malignant melanoma.

ACS Style

Sotiris Kyriakou; Melina Mitsiogianni; Theodora Mantso; William Cheung; Stephen Todryk; Stephany Veuger; Aglaia Pappa; David Tetard; Mihalis I. Panayiotidis. Anticancer activity of a novel methylated analogue of L-mimosine against an in vitro model of human malignant melanoma. Investigational New Drugs 2019, 38, 621 -633.

AMA Style

Sotiris Kyriakou, Melina Mitsiogianni, Theodora Mantso, William Cheung, Stephen Todryk, Stephany Veuger, Aglaia Pappa, David Tetard, Mihalis I. Panayiotidis. Anticancer activity of a novel methylated analogue of L-mimosine against an in vitro model of human malignant melanoma. Investigational New Drugs. 2019; 38 (3):621-633.

Chicago/Turabian Style

Sotiris Kyriakou; Melina Mitsiogianni; Theodora Mantso; William Cheung; Stephen Todryk; Stephany Veuger; Aglaia Pappa; David Tetard; Mihalis I. Panayiotidis. 2019. "Anticancer activity of a novel methylated analogue of L-mimosine against an in vitro model of human malignant melanoma." Investigational New Drugs 38, no. 3: 621-633.