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Surface active agents (SAAs), currently used in modern industry, are synthetic chemicals produced from non-renewable sources, with potential toxic impacts on humans and the environment. Thus, there is an increased interest for the identification and utilization of natural derived SAAs. As such, the marine environment is considered a promising source of biosurfactants with low toxicity, environmental compatibility, and biodegradation compared to their synthetic counterparts. MARISURF is a Horizon 2020 EU-funded project aiming to identify and functionally characterize SAAs, derived from a unique marine bacterial collection, towards commercial exploitation. Specifically, rhamnolipids produced by Marinobacter MCTG107b and Pseudomonas MCTG214(3b1) strains were previously identified and characterized while currently their toxicity profile was assessed by utilizing well-established methodologies. Our results showed a lack of cytotoxicity in in vitro models of human skin and liver as indicated by alamar blue and propidium iodide assays. Additionally, the use of the single gel electrophoresis assay, under oxidative stress conditions, revealed absence of any significant mutagenic/anti-mutagenic potential. Finally, both 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) cell-free assays, revealed no significant anti-oxidant capacity for neither of the tested compounds. Consequently, the absence of significant cytotoxicity and/or mutagenicity justifies their commercial exploitation and potential development into industrial end-user applications as natural and environmentally friendly biosurfactants.
Georgia-Persephoni Voulgaridou; Theodora Mantso; Ioannis Anestopoulos; Ariel Klavaris; Christina Katzastra; Despoina-Eugenia Kiousi; Marini Mantela; Alex Galanis; Konstantinos Gardikis; Ibrahim Banat; Tony Gutierrez; Karina Sałek; Stephen Euston; Mihalis Panayiotidis; Aglaia Pappa. Toxicity Profiling of Biosurfactants Produced by Novel Marine Bacterial Strains. International Journal of Molecular Sciences 2021, 22, 2383 .
AMA StyleGeorgia-Persephoni Voulgaridou, Theodora Mantso, Ioannis Anestopoulos, Ariel Klavaris, Christina Katzastra, Despoina-Eugenia Kiousi, Marini Mantela, Alex Galanis, Konstantinos Gardikis, Ibrahim Banat, Tony Gutierrez, Karina Sałek, Stephen Euston, Mihalis Panayiotidis, Aglaia Pappa. Toxicity Profiling of Biosurfactants Produced by Novel Marine Bacterial Strains. International Journal of Molecular Sciences. 2021; 22 (5):2383.
Chicago/Turabian StyleGeorgia-Persephoni Voulgaridou; Theodora Mantso; Ioannis Anestopoulos; Ariel Klavaris; Christina Katzastra; Despoina-Eugenia Kiousi; Marini Mantela; Alex Galanis; Konstantinos Gardikis; Ibrahim Banat; Tony Gutierrez; Karina Sałek; Stephen Euston; Mihalis Panayiotidis; Aglaia Pappa. 2021. "Toxicity Profiling of Biosurfactants Produced by Novel Marine Bacterial Strains." International Journal of Molecular Sciences 22, no. 5: 2383.
Propolis is a resinous substance produced by bees that exhibits antimicrobial, immunostimulatory and antioxidant activity. Its use is common in functional foods, cosmetics and traditional medicine despite the fact that it demonstrates low extraction yields and inconsistency in non-toxic solvents. In this work, a new encapsulation and delivery system consisting of liposomes and cyclodextrins incorporating propolis polyphenols has been developed and characterized. The antioxidant, antimutagenic and antiaging properties of the system under normal and UVB-induced oxidative stress conditions were investigated in cultured skin cells and/or reconstituted skin model. Furthermore, the transcript accumulation for an array of genes involved in many skin-related processes was studied. The system exhibits significant polyphenol encapsulation efficiency, physicochemical stability as well as controlled release rate in appropriate conditions. The delivery system can retain the anti-mutagenic, anti-oxidative and anti-ageing effects of propolis polyphenols to levels similar and comparable to those of propolis methanolic extracts, making the system ideal for applications where non-toxic solvents are required and controlled release of the polyphenol content is desired.
Eleni Spanidi; Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Sophia Letsiou; Nektarios Aligiannis; Ilias Tsochantaridis; Spyridon Kynigopoulos; Maria Lambropoulou; Ioannis Mourtzinos; Aglaia Pappa; Konstantinos Gardikis. A New Controlled Release System for Propolis Polyphenols and Its Biochemical Activity for Skin Applications. Plants 2021, 10, 420 .
AMA StyleEleni Spanidi, Athanasios Karapetsas, Georgia-Persephoni Voulgaridou, Sophia Letsiou, Nektarios Aligiannis, Ilias Tsochantaridis, Spyridon Kynigopoulos, Maria Lambropoulou, Ioannis Mourtzinos, Aglaia Pappa, Konstantinos Gardikis. A New Controlled Release System for Propolis Polyphenols and Its Biochemical Activity for Skin Applications. Plants. 2021; 10 (2):420.
Chicago/Turabian StyleEleni Spanidi; Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Sophia Letsiou; Nektarios Aligiannis; Ilias Tsochantaridis; Spyridon Kynigopoulos; Maria Lambropoulou; Ioannis Mourtzinos; Aglaia Pappa; Konstantinos Gardikis. 2021. "A New Controlled Release System for Propolis Polyphenols and Its Biochemical Activity for Skin Applications." Plants 10, no. 2: 420.
The antioxidant, cytoprotective, and wound-healing potential of the essential oil from the resin of Pistacia lentiscus var. chia (mastic oil) was evaluated, along with that of its major components, myrcene and α-pinene. Antioxidant potential was monitored as: (i) direct antioxidant activity as assessed by 2,2-di-phenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and ABTS assays; (ii) DNA damage protection activity; and (iii) cytoprotective activity as assessed via induction of transcription of genes related to the antioxidant response in human keratinocyte cells (HaCaT). The cytoprotective potential of the test substances was further evaluated against ultraviolet radiation B (UVB)- or H2O2-induced oxidative damage, whereas their regenerative capability was accessed by monitoring the wound closure rate in HaCaT. Μastic oil and major components did not show significant direct antioxidant activity, however they increased the mRNA levels of antioxidant response genes, suggesting indirect antioxidant activity. Treatment of HaCaT with the test substances before and after UVB irradiation resulted in increased cell viability in the cases of pre-treatment with mastic oil or post-treatment with myrcene. Increased cytoprotection was also observed in the case of cell treatment with mastic oil or its major components prior to H2O2 exposure. Finally, mastic oil and myrcene demonstrated a favorable dose-dependent effect for cell migration and wound closure. Collectively, mastic essential oil may exert its promising cytoprotective properties through indirect antioxidant mechanisms.
Vasileios Xanthis; Eleni Fitsiou; Georgia-Persephoni Voulgaridou; Athanasios Bogadakis; Katerina Chlichlia; Alex Galanis; Aglaia Pappa. Antioxidant and Cytoprotective Potential of the Essential Oil Pistacia lentiscus var. chia and Its Major Components Myrcene and α-Pinene. Antioxidants 2021, 10, 127 .
AMA StyleVasileios Xanthis, Eleni Fitsiou, Georgia-Persephoni Voulgaridou, Athanasios Bogadakis, Katerina Chlichlia, Alex Galanis, Aglaia Pappa. Antioxidant and Cytoprotective Potential of the Essential Oil Pistacia lentiscus var. chia and Its Major Components Myrcene and α-Pinene. Antioxidants. 2021; 10 (1):127.
Chicago/Turabian StyleVasileios Xanthis; Eleni Fitsiou; Georgia-Persephoni Voulgaridou; Athanasios Bogadakis; Katerina Chlichlia; Alex Galanis; Aglaia Pappa. 2021. "Antioxidant and Cytoprotective Potential of the Essential Oil Pistacia lentiscus var. chia and Its Major Components Myrcene and α-Pinene." Antioxidants 10, no. 1: 127.
Aldehyde dehydrogenases (ALDHs) are NAD(P)+-dependent enzymes that catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding carboxylic acids. ALDHs participate in a variety of cellular mechanisms, such as metabolism, cell proliferation and apoptosis, as well as differentiation and stemness. Over the last few years, ALDHs have emerged as cancer stem cell markers in a wide spectrum of solid tumors and hematological malignancies. In this study, the pathophysiological role of ALDH1B1 in human colorectal adenocarcinoma was investigated. Human colon cancer HT29 cells were stably transfected either with human green fluorescent protein (GFP)-tagged ALDH1B1 or with an empty lentiviral expression vector. The overexpression of ALDH1B1 was correlated with altered cell morphology, decreased proliferation rate and reduced clonogenic efficiency. Additionally, ALDH1B1 triggered a G2/M arrest at 24 h post-cell synchronization, probably through p53 and p21 upregulation. Furthermore, ALDH1B1-overexpressing HT29 cells exhibited enhanced resistance against doxorubicin, fluorouracil (5-FU) and etoposide. Finally, ALDH1B1 induced increased migratory potential and displayed epithelial–mesenchymal transition (EMT) through the upregulation of ZEB1 and vimentin and the consequent downregulation of E-cadherin. Taken together, ALDH1B1 confers alterations in the cell morphology, cell cycle progression and gene expression, accompanied by significant changes in the chemosensitivity and migratory potential of HT29 cells, underlying its potential significance in cancer progression.
Ilias Tsochantaridis; Angelos Roupas; Georgia-Persephoni Voulgaridou; Alexandra Giatromanolaki; Michael I. Koukourakis; Mihalis I. Panayiotidis; Aglaia Pappa. Aldehyde Dehydrogenase 1B1 Is Associated with Altered Cell Morphology, Proliferation, Migration and Chemosensitivity in Human Colorectal Adenocarcinoma Cells. Biomedicines 2021, 9, 44 .
AMA StyleIlias Tsochantaridis, Angelos Roupas, Georgia-Persephoni Voulgaridou, Alexandra Giatromanolaki, Michael I. Koukourakis, Mihalis I. Panayiotidis, Aglaia Pappa. Aldehyde Dehydrogenase 1B1 Is Associated with Altered Cell Morphology, Proliferation, Migration and Chemosensitivity in Human Colorectal Adenocarcinoma Cells. Biomedicines. 2021; 9 (1):44.
Chicago/Turabian StyleIlias Tsochantaridis; Angelos Roupas; Georgia-Persephoni Voulgaridou; Alexandra Giatromanolaki; Michael I. Koukourakis; Mihalis I. Panayiotidis; Aglaia Pappa. 2021. "Aldehyde Dehydrogenase 1B1 Is Associated with Altered Cell Morphology, Proliferation, Migration and Chemosensitivity in Human Colorectal Adenocarcinoma Cells." Biomedicines 9, no. 1: 44.
In the present study, we aimed to examine the antioxidant, antiaging and photoprotective properties of Greek honey samples of various botanical and geographical origin. Ethyl-acetate extracts were used and the and the total phenolic/flavonoid content and antioxidant capacity were evaluated. Honey extracts were then studied for their cytoprotective properties against UVB-induced photodamage using human immortalized keratinocytes (HaCaT) and/or reconstituted human skin tissue models. Specifically, the cytotoxicity, oxidative status, DNA damage and gene expression levels of specific matrix metalloproteinases (MMPs) were examined. Overall, the treatment of HaCaT cells with honey extracts resulted in lower levels of DNA strand breaks and attenuated the decrease in cell viability following UVB exposure. Additionally, honey extracts significantly decreased the total protein carbonyl content of the irradiated cells, however, they had no significant effect on their total antioxidant status. Finally, the extracts alleviated the UVB-induced up-regulation of MMPs-3, -7 and -9 in a model of reconstituted skin tissue. In conclusion, honey extracts exhibited significant photoprotective and antiaging properties under UVB exposure conditions and thus could be further exploited as promising agents for developing novel and naturally-based, antiaging cosmeceutical products.
Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Dimitra Iliadi; Ilias Tsochantaridis; Panagiota Michail; Spyridon Kynigopoulos; Maria Lambropoulou; Maria-Ioanna Stavropoulou; Konstantina Stathopoulou; Sofia Karabournioti; Nektarios Aligiannis; Konstantinos Gardikis; Alex Galanis; Mihalis I. Panayiotidis; Aglaia Pappa. Honey Extracts Exhibit Cytoprotective Properties against UVB-Induced Photodamage in Human Experimental Skin Models. Antioxidants 2020, 9, 566 .
AMA StyleAthanasios Karapetsas, Georgia-Persephoni Voulgaridou, Dimitra Iliadi, Ilias Tsochantaridis, Panagiota Michail, Spyridon Kynigopoulos, Maria Lambropoulou, Maria-Ioanna Stavropoulou, Konstantina Stathopoulou, Sofia Karabournioti, Nektarios Aligiannis, Konstantinos Gardikis, Alex Galanis, Mihalis I. Panayiotidis, Aglaia Pappa. Honey Extracts Exhibit Cytoprotective Properties against UVB-Induced Photodamage in Human Experimental Skin Models. Antioxidants. 2020; 9 (7):566.
Chicago/Turabian StyleAthanasios Karapetsas; Georgia-Persephoni Voulgaridou; Dimitra Iliadi; Ilias Tsochantaridis; Panagiota Michail; Spyridon Kynigopoulos; Maria Lambropoulou; Maria-Ioanna Stavropoulou; Konstantina Stathopoulou; Sofia Karabournioti; Nektarios Aligiannis; Konstantinos Gardikis; Alex Galanis; Mihalis I. Panayiotidis; Aglaia Pappa. 2020. "Honey Extracts Exhibit Cytoprotective Properties against UVB-Induced Photodamage in Human Experimental Skin Models." Antioxidants 9, no. 7: 566.
The aim of this study was to assess the antioxidant, photoprotective, and antiaging effects of Greek propolis. Propolis was subjected to n-heptane or methanol extraction. Total phenolic/flavonoid content and antioxidant potential were determined in the extracts. Promising extracts were evaluated for their cytoprotective properties using human immortalized keratinocyte (HaCaT) or reconstituted human skin tissue following exposure to UVB. Assessment of cytotoxicity, DNA damage, oxidative status, and gene/protein expression levels of various matrix metalloproteinases (MMPs) were performed. The propolis methanolic fractions exhibited higher total phenolic and flavonoid contents and significant in vitro antioxidant activity. Incubation of HaCaT cells with certain methanolic extracts significantly decreased the formation of DNA strand breaks following exposure to UVB and attenuated UVB-induced decrease in cell viability. The extracts had no remarkable effect on the total antioxidant status, but significantly lowered total protein carbonyl content used as a marker for protein oxidation in HaCaT cells. MMP-1, -3, -7, and -9, monitored as endpoints of antiaging efficacy, were significantly reduced by propolis following UVB exposure in a model of reconstituted skin tissue. In conclusion, propolis protects against the oxidative and photodamaging effects of UVB and could be further explored as a promising agent for developing natural antiaging strategies.
Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Manolis Konialis; Ilias Tsochantaridis; Spyridon Kynigopoulos; Maria Lambropoulou; Maria-Ioanna Stavropoulou; Konstantina Stathopoulou; Nektarios Aligiannis; Petros Bozidis; Anna Goussia; Konstantinos Gardikis; Mihalis I. Panayiotidis; Aglaia Pappa. Propolis Extracts Inhibit UV-Induced Photodamage in Human Experimental In Vitro Skin Models. Antioxidants 2019, 8, 125 .
AMA StyleAthanasios Karapetsas, Georgia-Persephoni Voulgaridou, Manolis Konialis, Ilias Tsochantaridis, Spyridon Kynigopoulos, Maria Lambropoulou, Maria-Ioanna Stavropoulou, Konstantina Stathopoulou, Nektarios Aligiannis, Petros Bozidis, Anna Goussia, Konstantinos Gardikis, Mihalis I. Panayiotidis, Aglaia Pappa. Propolis Extracts Inhibit UV-Induced Photodamage in Human Experimental In Vitro Skin Models. Antioxidants. 2019; 8 (5):125.
Chicago/Turabian StyleAthanasios Karapetsas; Georgia-Persephoni Voulgaridou; Manolis Konialis; Ilias Tsochantaridis; Spyridon Kynigopoulos; Maria Lambropoulou; Maria-Ioanna Stavropoulou; Konstantina Stathopoulou; Nektarios Aligiannis; Petros Bozidis; Anna Goussia; Konstantinos Gardikis; Mihalis I. Panayiotidis; Aglaia Pappa. 2019. "Propolis Extracts Inhibit UV-Induced Photodamage in Human Experimental In Vitro Skin Models." Antioxidants 8, no. 5: 125.
In the present study, we have aimed to characterize the intrinsic, extrinsic and ER-mediated apoptotic induction by hyperthermia in an in vitro model of human malignant melanoma and furthermore, to evaluate its therapeutic effectiveness in an adjuvant therapeutic setting characterized by combinational treatments with non-targeted (Dacarbazine & Temozolomide) and targeted (Dabrafenib & Vemurafenib) drugs. Overall, our data showed that both low (43 °C) and high (45 °C) hyperthermic exposures were capable of inducing cell death by activating all apoptotic pathways but in a rather distinct manner. More specifically, low hyperthermia induced extrinsic and intrinsic apoptotic pathways both of which activated caspase 6 only as opposed to high hyperthermia which was mediated by the combined effects of caspases 3, 7 and 6. Furthermore, significant involvement of the ER was evident (under both hyperthermic conditions) suggesting its role in regulating apoptosis via activation of CHOP. Our data revealed that while low hyperthermia activated IRE-1 and ATF6 only, high hyperthermia induced activation of PERK as well suggesting that ultimately these ER stress sensors can lead to the induction of CHOP via different pathways of transmitted signals. Finally, combinational treatment protocols revealed an effect of hyperthermia in potentiating the therapeutic effectiveness of non-targeted as well as targeted drugs utilized in the clinical setting. Overall, our findings support evidence into hyperthermia’s therapeutic potential in treating human malignant melanoma by elucidating the underlying mechanisms of its complex apoptotic induction.
T. Mantso; S. Vasileiadis; I. Anestopoulos; Georgia-Persephoni Voulgaridou; E. Lampri; S. Botaitis; E. N. Kontomanolis; C. Simopoulos; G. Goussetis; Rodrigo Franco; K. Chlichlia; Aglaia Pappa; M. I. Panayiotidis. Hyperthermia induces therapeutic effectiveness and potentiates adjuvant therapy with non-targeted and targeted drugs in an in vitro model of human malignant melanoma. Scientific Reports 2018, 8, 1 -16.
AMA StyleT. Mantso, S. Vasileiadis, I. Anestopoulos, Georgia-Persephoni Voulgaridou, E. Lampri, S. Botaitis, E. N. Kontomanolis, C. Simopoulos, G. Goussetis, Rodrigo Franco, K. Chlichlia, Aglaia Pappa, M. I. Panayiotidis. Hyperthermia induces therapeutic effectiveness and potentiates adjuvant therapy with non-targeted and targeted drugs in an in vitro model of human malignant melanoma. Scientific Reports. 2018; 8 (1):1-16.
Chicago/Turabian StyleT. Mantso; S. Vasileiadis; I. Anestopoulos; Georgia-Persephoni Voulgaridou; E. Lampri; S. Botaitis; E. N. Kontomanolis; C. Simopoulos; G. Goussetis; Rodrigo Franco; K. Chlichlia; Aglaia Pappa; M. I. Panayiotidis. 2018. "Hyperthermia induces therapeutic effectiveness and potentiates adjuvant therapy with non-targeted and targeted drugs in an in vitro model of human malignant melanoma." Scientific Reports 8, no. 1: 1-16.
Aldehyde dehydrogenase 3A1 (ALDH3A1) is a metabolic enzyme that catalyzes the oxidation of various aldehydes. Certain types of epithelial tissues in mammals, especially those continually exposed to environmental stress (e.g., corneal epithelium), express ALDH3A1 at high levels and its abundance in such tissues is perceived to help to maintain cellular homeostasis under conditions of oxidative stress. Metabolic as well as non-metabolic roles for ALDH3A1 have been associated with its mediated resistance to cellular oxidative stress. In this study, we provide evidence that ALDH3A1 exhibits molecular chaperone-like activity further supporting its multifunctional role. Specifically, we expressed and purified the human ALDH3A1 in E. coli and used the recombinant protein to investigate its in vitro ability to protect SmaI and citrate synthase (from precipitation and/or deactivation) under thermal stress conditions. Our results indicate that recombinant ALDH3A1 exhibits significant chaperone function in vitro. Furthermore, over-expression of the fused histidine-tagged ALDH3A1 confers host E. coli cells with enhanced resistance to thermal shock, while ALDH3A1 over-expression in the human corneal cell line HCE-2 was sufficient for protecting them from the cytotoxic effects of both hydrogen peroxide and tert-butyl hydroperoxide. These results further support the chaperone-like function of human ALDH3A1. Taken together, ALDH3A1, in addition to its primary metabolic role in fundamental cellular detoxification processes, appears to play an essential role in protecting cellular proteins against aggregation under stress conditions.
Georgia-Persephoni Voulgaridou; Ilias Tsochantaridis; Theodora Mantso; Rodrigo Franco; Mihalis I. Panayiotidis; Aglaia Pappa. Human aldehyde dehydrogenase 3A1 (ALDH3A1) exhibits chaperone-like function. The International Journal of Biochemistry & Cell Biology 2017, 89, 16 -24.
AMA StyleGeorgia-Persephoni Voulgaridou, Ilias Tsochantaridis, Theodora Mantso, Rodrigo Franco, Mihalis I. Panayiotidis, Aglaia Pappa. Human aldehyde dehydrogenase 3A1 (ALDH3A1) exhibits chaperone-like function. The International Journal of Biochemistry & Cell Biology. 2017; 89 ():16-24.
Chicago/Turabian StyleGeorgia-Persephoni Voulgaridou; Ilias Tsochantaridis; Theodora Mantso; Rodrigo Franco; Mihalis I. Panayiotidis; Aglaia Pappa. 2017. "Human aldehyde dehydrogenase 3A1 (ALDH3A1) exhibits chaperone-like function." The International Journal of Biochemistry & Cell Biology 89, no. : 16-24.
Aldehyde dehydrogenases participate in a variety of cellular homeostatic mechanisms like metabolism, proliferation, differentiation, apoptosis, whereas recently, they have been implicated in normal and cancer cell stemness. We explored roles for ALDH3A1 in conferring resistance to chemotherapeutics/radiation/oxidative stress and whether ectopic overexpression of ALDH3A1 could lead to alterations of gene expression profile associated with cancer stem cell-like phenotype. MCF-7 cells were stably transfected either with an empty vector (mock) or human aldehyde dehydrogenase 3A1 cDNA. The expression of aldehyde dehydrogenase 3A1 in MCF-7 cells was associated with altered cell proliferation rate and enhanced cell resistance against various chemotherapeutic drugs (4-hydroxyperoxycyclophosphamide, doxorubicin, etoposide, and 5-fluorouracil). Aldehyde dehydrogenase 3A1 expression also led to increased tolerance of MCF-7 cells to gamma radiation and hydrogen peroxide-induced stress. Furthermore, aldehyde dehydrogenase 3A1-expressing MCF-7 cells exhibited gene up-regulation of cyclins A, B1, B2, and down-regulation of cyclin D1 as well as transcription factors p21, CXR4, Notch1, SOX2, SOX4, OCT4, and JAG1. When compared to mock cells, no changes were observed in mRNA levels of ABCA2 and ABCB1 protein pumps with only a minor decrease of the ABCG2 pump in the aldehyde dehydrogenase 3A1-expressing cells. Also, the adhesion molecules EpCAM and CD49F were also found to be up-regulated in aldehyde dehydrogenase 3A1expressing cells. Taken together, ALDH3A1 confers a multi-modality resistance phenotype in MCF-7 cells associated with slower growth rate, increased clonogenic capacity, and altered gene expression profile, underlining its significance in cell homeostasis.
Georgia-Persephoni Voulgaridou; Magdalini Kiziridou; Theodora Mantso; Katerina Chlichlia; Alex Galanis; Michael I. Koukourakis; Rodrigo Franco; Mihalis I. Panayiotidis; Aglaia Pappa. Aldehyde dehydrogenase 3A1 promotes multi-modality resistance and alters gene expression profile in human breast adenocarcinoma MCF-7 cells. The International Journal of Biochemistry & Cell Biology 2016, 77, 120 -128.
AMA StyleGeorgia-Persephoni Voulgaridou, Magdalini Kiziridou, Theodora Mantso, Katerina Chlichlia, Alex Galanis, Michael I. Koukourakis, Rodrigo Franco, Mihalis I. Panayiotidis, Aglaia Pappa. Aldehyde dehydrogenase 3A1 promotes multi-modality resistance and alters gene expression profile in human breast adenocarcinoma MCF-7 cells. The International Journal of Biochemistry & Cell Biology. 2016; 77 ():120-128.
Chicago/Turabian StyleGeorgia-Persephoni Voulgaridou; Magdalini Kiziridou; Theodora Mantso; Katerina Chlichlia; Alex Galanis; Michael I. Koukourakis; Rodrigo Franco; Mihalis I. Panayiotidis; Aglaia Pappa. 2016. "Aldehyde dehydrogenase 3A1 promotes multi-modality resistance and alters gene expression profile in human breast adenocarcinoma MCF-7 cells." The International Journal of Biochemistry & Cell Biology 77, no. : 120-128.
This paper presents the experimental configuration and procedure as well as the in-vitro assessment of Jurkat T-cells response to 1966 MHz exposure of modulated and unmodulated electromagnetic signals within a Gigahertz Transverse Electro-Magnetic (GTEM) cell. Different combinations of electric field intensity, exposure duration and modulation schemes were applied. Exposures at continuous wave (CW) signal at low intensity levels (3 V/m) did not induce any significant DNA damage, but a slight increase was observed for extreme stress levels (76.4 V/m). On the other hand, the results indicate that, at both, low and high electric field intensity UMTS (Universal Mobile Telecommunications System) signal could be statistically related to DNA damage in-vitro. Nevertheless, further experiments are required, increasing the statistical number of samples and recruiting more DNA damage endpoints before conclusive statements are drawn.
Nektarios Moraitis; Maria Christopoulou; Konstantina S. Nikita; Georgia-Persephoni Voulgaridou; Ioannis Anestopoulos; Mihalis I. Panagiotidis; Aglaia Pappa. In-vitro assessment of Jurkat T-cells response to 1966 MHz electromagnetic fields in a GTEM cell. 2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) 2015, 2015, 2592 -2595.
AMA StyleNektarios Moraitis, Maria Christopoulou, Konstantina S. Nikita, Georgia-Persephoni Voulgaridou, Ioannis Anestopoulos, Mihalis I. Panagiotidis, Aglaia Pappa. In-vitro assessment of Jurkat T-cells response to 1966 MHz electromagnetic fields in a GTEM cell. 2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). 2015; 2015 ():2592-2595.
Chicago/Turabian StyleNektarios Moraitis; Maria Christopoulou; Konstantina S. Nikita; Georgia-Persephoni Voulgaridou; Ioannis Anestopoulos; Mihalis I. Panagiotidis; Aglaia Pappa. 2015. "In-vitro assessment of Jurkat T-cells response to 1966 MHz electromagnetic fields in a GTEM cell." 2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) 2015, no. : 2592-2595.
Hepatocellular carcinoma (HCC) is the most common type of liver malignancy and one with high fatality. Its 5-year survival rate remains low and thus, there is a need for improvement of current treatment strategies as well as development of novel targeted methodologies in order to optimize existing therapeutic protocols. To this end, only recently, it was discovered that its pathophysiology also involves epigenetic alterations in DNA methylation, histone modifications and/or non-coding microRNA patterns. Unlike genetic events, epigenetic alterations are reversible and thus potentially considered to be an alternative option in cancer treatment protocols. In this review, we describe the general characteristics and resulted major alterations of the epigenetic machinery as well as current state of progress of epigenetic therapy (via different single or combinatorial experimental approaches) in HCC.
Ioannis Anestopoulos; Georgia Persephoni Voulgaridou; Alexandros G. Georgakilas; Rodrigo Franco; Aglaia Pappa; Mihalis I. Panayiotidis. Epigenetic therapy as a novel approach in hepatocellular carcinoma. Pharmacology & Therapeutics 2015, 145, 103 -119.
AMA StyleIoannis Anestopoulos, Georgia Persephoni Voulgaridou, Alexandros G. Georgakilas, Rodrigo Franco, Aglaia Pappa, Mihalis I. Panayiotidis. Epigenetic therapy as a novel approach in hepatocellular carcinoma. Pharmacology & Therapeutics. 2015; 145 ():103-119.
Chicago/Turabian StyleIoannis Anestopoulos; Georgia Persephoni Voulgaridou; Alexandros G. Georgakilas; Rodrigo Franco; Aglaia Pappa; Mihalis I. Panayiotidis. 2015. "Epigenetic therapy as a novel approach in hepatocellular carcinoma." Pharmacology & Therapeutics 145, no. : 103-119.
Aldehyde dehydrogenase 3A1 (ALDH3A1) is a recently characterized corneal crystallin with its exact functions still being unclear. Expressing recombinant human ALDH3A1 has been difficult in Escherichia coli (E. coli) because of low solubility, yield and insufficient purity issues. In this report, we compared different E. coli expression strategies (namely the maltose binding protein; MBP- and the 6-his-tagged expression systems) under conditions of auto-induction and co-expression with E. coli’s molecular chaperones where appropriate. Thus, we aimed to screen the efficiency of these expression strategies in order to improve solubility of recombinant ALDH3A1 when expressed in E. coli. We showed that the MBP- tagged expression in combination with lower-temperature culture conditions resulted in active soluble recombinant ALDH3A1. Expression of the fused 6-his tagged-ALDH3A1 protein resulted in poor solubility and neither lowering temperature culture conditions nor the auto-induction strategy improved its solubility. Furthermore, higher yield of soluble, active native form of 6-his tagged-ALDH3A1 was facilitated through co-expression of the two groups of E. coli’s molecular chaperones, GroES/GroEL and DnaK/DnaJ/GrpE. Convenient one step immobilized affinity chromatography methods were utilized to purify the fused ALDH3A1 hybrids. Both fusion proteins retained their biological activity and could be used directly without removing the fusion tags. Taken together, our results provide a rational option for producing sufficient amounts of soluble and active recombinant ALDH3A1 using the E. coli expression system for conducting functional studies towards elucidating the biological role(s) of this interesting corneal crystallin.
Georgia-Persephoni Voulgaridou; Theodora Mantso; Katerina Chlichlia; Mihalis I. Panayiotidis; Aglaia Pappa. Efficient E. coli Expression Strategies for Production of Soluble Human Crystallin ALDH3A1. PLOS ONE 2013, 8, e56582 .
AMA StyleGeorgia-Persephoni Voulgaridou, Theodora Mantso, Katerina Chlichlia, Mihalis I. Panayiotidis, Aglaia Pappa. Efficient E. coli Expression Strategies for Production of Soluble Human Crystallin ALDH3A1. PLOS ONE. 2013; 8 (2):e56582.
Chicago/Turabian StyleGeorgia-Persephoni Voulgaridou; Theodora Mantso; Katerina Chlichlia; Mihalis I. Panayiotidis; Aglaia Pappa. 2013. "Efficient E. coli Expression Strategies for Production of Soluble Human Crystallin ALDH3A1." PLOS ONE 8, no. 2: e56582.
Cancer is a multistage process where each stage involves different molecular, biochemical and cellular events all of which, however, contribute to malignant transformation. Over the last years, substantial scientific evidence has promoted the hypothesis that ROS-induced cellular damage underlies key steps during development of the malignant phenotype including evasion of apoptosis, limitless proliferation, angiogenesis, tissue invasion and metastasis, etc. On the other hand, natural products hold great promise as anti-cancer compounds in preventing against carcinogenesis both in vitro and in vivo. Throughout this article, we aim to review the evidence as to how some of these natural products exert their chemopreventive effects in human carcinogenesis. For this reason, we have placed particular emphasis on oral cancer where significant efforts have been made in alternative therapeutic strategies such as the use of plant-derived natural products. This is of paramount importance given the disease's high morbidity and mortality rates across the world and specifically in the geographic regions of India and South-East Asia where its incidence is increasing.
Dominique Ziech; Ioannis Anestopoulos; Rania Hanafi; Georgia-Persephoni Voulgaridou; Rodrigo Franco; Alexandros G. Georgakilas; Aglaia Pappa; Mihalis I. Panayiotidis. Pleiotrophic effects of natural products in ROS-induced carcinogenesis: The role of plant-derived natural products in oral cancer chemoprevention. Cancer Letters 2012, 327, 16 -25.
AMA StyleDominique Ziech, Ioannis Anestopoulos, Rania Hanafi, Georgia-Persephoni Voulgaridou, Rodrigo Franco, Alexandros G. Georgakilas, Aglaia Pappa, Mihalis I. Panayiotidis. Pleiotrophic effects of natural products in ROS-induced carcinogenesis: The role of plant-derived natural products in oral cancer chemoprevention. Cancer Letters. 2012; 327 (1-2):16-25.
Chicago/Turabian StyleDominique Ziech; Ioannis Anestopoulos; Rania Hanafi; Georgia-Persephoni Voulgaridou; Rodrigo Franco; Alexandros G. Georgakilas; Aglaia Pappa; Mihalis I. Panayiotidis. 2012. "Pleiotrophic effects of natural products in ROS-induced carcinogenesis: The role of plant-derived natural products in oral cancer chemoprevention." Cancer Letters 327, no. 1-2: 16-25.
Oral cancer accounts for 2-3% of all malignancies and according to the World Health Organization (WHO) is the fifth most common cancer worldwide. On the other hand, “oxidative stress” implies a cellular state whereby reactive oxygen species (ROS) production exceeds its metabolism resulting in excessive ROS accumulation and overwhelmed cellular defenses. Such a state has been shown to be involved in the multistage process of human carcinogenesis (including oral cancer) via many different mechanisms. Amongst them are ROS-induced oxidative modifications on major cellular macromolecules like DNA, proteins and lipids with the resulting byproducts being involved in the pathophysiology of human oral malignant and pre-malignant lesions. Throughout this manuscript, we review the current state of knowledge on the role of these oxidativemodified cellular byproducts in serving as reliable biomarkers for oral cancer detection, prognosis and diagnosis.
R. Hanafi; I. Anestopoulos; Georgia-Persephoni Voulgaridou; Rodrigo Franco; A. G. Georgakilas; D. Ziech; V. Malamou-Mitsi; Aglaia Pappa; M. I. Panayiotidis. Oxidative Stress Based-Biomarkers in Oral Carcinogenesis: How Far Have We Gone? Current Molecular Medicine 2012, 12, 698 -703.
AMA StyleR. Hanafi, I. Anestopoulos, Georgia-Persephoni Voulgaridou, Rodrigo Franco, A. G. Georgakilas, D. Ziech, V. Malamou-Mitsi, Aglaia Pappa, M. I. Panayiotidis. Oxidative Stress Based-Biomarkers in Oral Carcinogenesis: How Far Have We Gone? Current Molecular Medicine. 2012; 12 (6):698-703.
Chicago/Turabian StyleR. Hanafi; I. Anestopoulos; Georgia-Persephoni Voulgaridou; Rodrigo Franco; A. G. Georgakilas; D. Ziech; V. Malamou-Mitsi; Aglaia Pappa; M. I. Panayiotidis. 2012. "Oxidative Stress Based-Biomarkers in Oral Carcinogenesis: How Far Have We Gone?" Current Molecular Medicine 12, no. 6: 698-703.
DNA damage plays a major role in various pathophysiological conditions including carcinogenesis, aging, inflammation, diabetes and neurodegenerative diseases. Oxidative stress and cell processes such as lipid peroxidation and glycation induce the formation of highly reactive endogenous aldehydes that react directly with DNA, form aldehyde-derived DNA adducts and lead to DNA damage. In occasion of persistent conditions that influence the formation and accumulation of aldehyde-derived DNA adducts the resulting unrepaired DNA damage causes deregulation of cell homeostasis and thus significantly contributes to disease phenotype. Some of the most highly reactive aldehydes produced endogenously are 4-hydroxy-2-nonenal, malondialdehyde, acrolein, crotonaldehyde and methylglyoxal. The mutagenic and carcinogenic effects associated with the elevated levels of these reactive aldehydes, especially, under conditions of stress, are attributed to their capability of causing directly modification of DNA bases or yielding promutagenic exocyclic adducts. In this review, we discuss the current knowledge on DNA damage induced by endogenously produced reactive aldehydes in relation to the pathophysiology of human diseases.
Georgia-Persephoni Voulgaridou; Ioannis Anestopoulos; Rodrigo Franco; Mihalis I. Panayiotidis; Aglaia Pappa. DNA damage induced by endogenous aldehydes: Current state of knowledge. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 2011, 711, 13 -27.
AMA StyleGeorgia-Persephoni Voulgaridou, Ioannis Anestopoulos, Rodrigo Franco, Mihalis I. Panayiotidis, Aglaia Pappa. DNA damage induced by endogenous aldehydes: Current state of knowledge. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 2011; 711 (1-2):13-27.
Chicago/Turabian StyleGeorgia-Persephoni Voulgaridou; Ioannis Anestopoulos; Rodrigo Franco; Mihalis I. Panayiotidis; Aglaia Pappa. 2011. "DNA damage induced by endogenous aldehydes: Current state of knowledge." Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 711, no. 1-2: 13-27.