Biodiversity is a concept of great scientific interest and social value studied in different subjects of the secondary education curriculum. Citizen–science programs may contribute to increasing the engagement of students when studying biodiversity. This work aimed to explore the use of the citizen–science platform iNaturalist as a complement of the elaboration of herbaria in an outdoor activity for 4th course 16-year-old students in the Basaula Reserve. The platform iNaturalist was chosen for its suitability to develop collaborative projects in an educational context. The Basaula project was created and 122 students were trained to record plant species in an outdoor activity. A total of 32 species were recorded, among them the most abundant were beech (Fagus sylvatica) and holm oak (Quercus ilex). The students positively evaluated their experience, highlighting its adequacy to record biodiversity data and make a virtual herbarium. Students valued the innovative character of iNaturalist and its usefulness for research but also the opportunity to integrate mobile devices in school education. We concluded that iNaturalist is a valuable tool to carry out collaborative projects dealing with biodiversity in secondary education.

Drought is an environmental stressor that affects crop yield worldwide. Understanding plant physiological responses to stress conditions is needed to secure food in future climate conditions. In this study, we applied a combination of plant physiology and metabolomic techniques to understand plant responses to progressive water deficit focusing on the root system. We chose two legume plants with contrasting tolerance to drought, the widely cultivated alfalfa Medicago sativa (Ms) and the model legume Medicago truncatula (Mt) for comparative analysis. Ms taproot (tapR) and Mt fibrous root (fibR) biomass increased during drought, while a progressive decline in water content was observed in both species. Metabolomic analysis allowed the identification of key metabolites in the different tissues tested. Under drought, carbohydrates, abscisic acid, and proline predominantly accumulated in leaves and tapRs, whereas flavonoids increased in fibRs in both species. Raffinose-family related metabolites accumulated during drought. Along with an accumulation of root sucrose in plants subjected to drought, both species showed a decrease in sucrose synthase (SUS) activity related to a reduction in the transcript level of SUS1, the main SUS gene. This study highlights the relevance of root carbon metabolism during drought conditions and provides evidence on the specific accumulation of metabolites throughout the root system.

The aim of this study was to assess the utility of Sentinel-2 images in the monitoring of the fractional vegetation cover (FVC) of rainfed alfalfa in semiarid areas such as that of Bardenas Reales in Spain. FVC was sampled in situ using 1 m² surfaces at 172 points inside 18 alfalfa fields from late spring to early summer in 2017 and 2018. Different vegetation indices derived from a series of Sentinel-2 images were calculated and were then correlated with the FVC measurements at the pixel and parcel levels using different types of equations. The results indicate that the normalized difference vegetation index (NDVI) and FVC were highly correlated at the parcel level (R² = 0.712), whereas the correlation at the pixel level remained moderate across each of the years studied. Based on the findings, another 29 alfalfa plots (28 rainfed; 1 irrigated) were remotely monitored operationally for 3 years (2017–2019), revealing that location and weather conditions were strong determinants of alfalfa growth in Bardenas Reales. The results of this study indicate that Sentinel-2 imagery is a suitable tool for monitoring rainfed alfalfa pastures in semiarid areas, thus increasing the potential success of pasture management.

Legumes are the second most important crop family in the world representing around the 15% of the cultivated area. They are ecologically relevant because its capacity to fix atmospheric nitrogen and represent one of the most relevant inputs of protein for human and animal feed. Between them, alfalfa (Medicago sativa, Ms) is one of the most well-known and widely cultivated worldwide, while Medicago truncatula (Mt), which is phylogenetically related to alfalfa is naturally present in the Mediterranean semiarid area. In a climate change scenario, where drought is becoming more serious, and world population increases year by year, there is a concern for food availability both for humans and animals, and these two species are key in reaching this food requirements. To meet food security goals is important to know plant responses under drought stress. The general aim of this work is to understand the responses of Ms and Mt to drought conditions, focusing mainly in carbon metabolism at root level. In this context, we included a rainfed alfalfa study at the field level in Bardenas Reales.