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Dr. Idoia Ariz
Institute for Multidisciplinary Applied Biology (IMAB), Campus Arrosadia s/n, Public University of Navarra, 31006 Pamplona, Navarra, Spain

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Research Keywords & Expertise

0 Nitrogen metabolism
0 nitrogen fertilization
0 Ammonium nutrition
0 Ammonium transport
0 Natural N isotope abundance in crops

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Ammonium transport

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Journal article
Published: 14 January 2021 in Sustainability
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Biodiversity is a concept of great scientific interest and social value studied in different subjects of the secondary education curriculum. Citizen–science programs may contribute to increasing the engagement of students when studying biodiversity. This work aimed to explore the use of the citizen–science platform iNaturalist as a complement of the elaboration of herbaria in an outdoor activity for 4th course 16-year-old students in the Basaula Reserve. The platform iNaturalist was chosen for its suitability to develop collaborative projects in an educational context. The Basaula project was created and 122 students were trained to record plant species in an outdoor activity. A total of 32 species were recorded, among them the most abundant were beech (Fagus sylvatica) and holm oak (Quercus ilex). The students positively evaluated their experience, highlighting its adequacy to record biodiversity data and make a virtual herbarium. Students valued the innovative character of iNaturalist and its usefulness for research but also the opportunity to integrate mobile devices in school education. We concluded that iNaturalist is a valuable tool to carry out collaborative projects dealing with biodiversity in secondary education.

ACS Style

Andres Echeverria; Idoia Ariz; Judit Moreno; Javier Peralta; Esther Gonzalez. Learning Plant Biodiversity in Nature: The Use of the Citizen–Science Platform iNaturalist as a Collaborative Tool in Secondary Education. Sustainability 2021, 13, 735 .

AMA Style

Andres Echeverria, Idoia Ariz, Judit Moreno, Javier Peralta, Esther Gonzalez. Learning Plant Biodiversity in Nature: The Use of the Citizen–Science Platform iNaturalist as a Collaborative Tool in Secondary Education. Sustainability. 2021; 13 (2):735.

Chicago/Turabian Style

Andres Echeverria; Idoia Ariz; Judit Moreno; Javier Peralta; Esther Gonzalez. 2021. "Learning Plant Biodiversity in Nature: The Use of the Citizen–Science Platform iNaturalist as a Collaborative Tool in Secondary Education." Sustainability 13, no. 2: 735.

Journal article
Published: 14 February 2019 in International Journal of Molecular Sciences
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Nitrogen is an essential element for plant nutrition. Nitrate and ammonium are the two major inorganic nitrogen forms available for plant growth. Plant preference for one or the other form depends on the interplay between plant genetic background and environmental variables. Ammonium-based fertilization has been shown less environmentally harmful compared to nitrate fertilization, because of reducing, among others, nitrate leaching and nitrous oxide emissions. However, ammonium nutrition may become a stressful situation for a wide range of plant species when the ion is present at high concentrations. Although studied for long time, there is still an important lack of knowledge to explain plant tolerance or sensitivity towards ammonium nutrition. In this context, we performed a comparative proteomic study in roots of Arabidopsis thaliana plants grown under exclusive ammonium or nitrate supply. We identified and quantified 68 proteins with differential abundance between both conditions. These proteins revealed new potential important players on root response to ammonium nutrition, such as H+-consuming metabolic pathways to regulate pH homeostasis and specific secondary metabolic pathways like brassinosteroid and glucosinolate biosynthetic pathways.

ACS Style

Inmaculada Coleto; Izargi Vega-Mas; Gaetan Glauser; Gonzalez Moro; Daniel Marino; Idoia Ariz. New Insights on Arabidopsis thaliana Root Adaption to Ammonium Nutrition by the Use of a Quantitative Proteomic Approach. International Journal of Molecular Sciences 2019, 20, 814 .

AMA Style

Inmaculada Coleto, Izargi Vega-Mas, Gaetan Glauser, Gonzalez Moro, Daniel Marino, Idoia Ariz. New Insights on Arabidopsis thaliana Root Adaption to Ammonium Nutrition by the Use of a Quantitative Proteomic Approach. International Journal of Molecular Sciences. 2019; 20 (4):814.

Chicago/Turabian Style

Inmaculada Coleto; Izargi Vega-Mas; Gaetan Glauser; Gonzalez Moro; Daniel Marino; Idoia Ariz. 2019. "New Insights on Arabidopsis thaliana Root Adaption to Ammonium Nutrition by the Use of a Quantitative Proteomic Approach." International Journal of Molecular Sciences 20, no. 4: 814.

Research article
Published: 12 September 2018 in Science Advances
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Ammonium is an important nitrogen (N) source for living organisms, a key metabolite for pH control, and a potent cytotoxic compound. Ammonium is transported by the widespread AMT-Mep-Rh membrane proteins, and despite their significance in physiological processes, the nature of substrate translocation (NH3/NH4 +) by the distinct members of this family is still a matter of controversy. Using Saccharomyces cerevisiae cells expressing representative AMT-Mep-Rh ammonium carriers and taking advantage of the natural chemical-physical property of the N isotopic signature linked to NH4 +/NH3 conversion, this study shows that only cells expressing AMT-Mep-Rh proteins were depleted in 15N relative to 14N when compared to the external ammonium source. We observed 15N depletion over a wide range of external pH, indicating its independence of NH3 formation in solution. On the basis of inhibitor studies, ammonium transport by nonspecific cation channels did not show isotope fractionation but competition with K+. We propose that kinetic N isotope fractionation is a common feature of AMT-Mep-Rh–type proteins, which favor 14N over 15N, owing to the dissociation of NH4 + into NH3 + H+ in the protein, leading to 15N depletion in the cell and allowing NH3 passage or NH3/H+ cotransport. This deprotonation mechanism explains these proteins’ essential functions in environments under a low NH4 +/K+ ratio, allowing organisms to specifically scavenge NH4 +. We show that 15N isotope fractionation may be used in vivo not only to determine the molecular species being transported by ammonium transport proteins, but also to track ammonium toxicity and associated amino acids excretion.

ACS Style

Idoia Ariz; Mélanie Boeckstaens; Catarina Gouveia; Ana Paula Martins; Emanuel Sanz-Luque; Emilio Fernández; Graça Soveral; Nicolaus von Wirén; Anna M. Marini; Pedro M. Aparicio-Tejo; Cristina Cruz. Nitrogen isotope signature evidences ammonium deprotonation as a common transport mechanism for the AMT-Mep-Rh protein superfamily. Science Advances 2018, 4, eaar3599 .

AMA Style

Idoia Ariz, Mélanie Boeckstaens, Catarina Gouveia, Ana Paula Martins, Emanuel Sanz-Luque, Emilio Fernández, Graça Soveral, Nicolaus von Wirén, Anna M. Marini, Pedro M. Aparicio-Tejo, Cristina Cruz. Nitrogen isotope signature evidences ammonium deprotonation as a common transport mechanism for the AMT-Mep-Rh protein superfamily. Science Advances. 2018; 4 (9):eaar3599.

Chicago/Turabian Style

Idoia Ariz; Mélanie Boeckstaens; Catarina Gouveia; Ana Paula Martins; Emanuel Sanz-Luque; Emilio Fernández; Graça Soveral; Nicolaus von Wirén; Anna M. Marini; Pedro M. Aparicio-Tejo; Cristina Cruz. 2018. "Nitrogen isotope signature evidences ammonium deprotonation as a common transport mechanism for the AMT-Mep-Rh protein superfamily." Science Advances 4, no. 9: eaar3599.

Review
Published: 01 July 2016 in Plant Science
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Ammonium sensitivity of plants is a worldwide problem, constraining crop production. Prolonged application of ammonium as the sole nitrogen source may result in physiological and morphological disorders that lead to decreased plant growth and toxicity. The main causes of ammonium toxicity/tolerance described until now include high ammonium assimilation by plants and/or low sensitivity to external pH acidification. The various ammonium transport-related components, especially the non-electrogenic influx of NH3 (related to the depletion of 15N) and the electrogenic influx of NH4+, may contribute to ammonium accumulation, and therefore to NH3 toxicity. However, this accumulation may be influenced by increasing K+ concentration in the root medium. Recently, new insights have been provided by “omics” studies, leading to a suggested involvement of GDP mannose-pyrophosphorylase in the response pathways of NH4+ stress. In this review, we highlight the cross-talk signaling between nitrate, auxins and NO, and the importance of the connection of the plants’ urea cycle to metabolism of polyamines. Overall, the tolerance and amelioration of ammonium toxicity are outlined to improve the yield of ammonium-grown plants. This review identifies future directions of research, focusing on the putative importance of aquaporins in ammonium influx, and on genes involved in ammonium sensitivity and tolerance.The authors acknowledge the support of research grants AGL2014-52396-P from the Spanish Ministry of Economy and Competitiveness (MINECO) and PTDC/BIA-BEC/099323/2008 and PTDC/BIA-ECS/122214/2010 from the Portuguese Fundação para a Ciência e Tecnologia (FCT). RE received a JAE-Doc-2011-046 fellow from the Spanish CSIC, co-financed by the European Social Fund

ACS Style

Raquel Esteban; Idoia Ariz; Cristina Cruz; Jose Fernando Moran. Review: Mechanisms of ammonium toxicity and the quest for tolerance. Plant Science 2016, 248, 92 -101.

AMA Style

Raquel Esteban, Idoia Ariz, Cristina Cruz, Jose Fernando Moran. Review: Mechanisms of ammonium toxicity and the quest for tolerance. Plant Science. 2016; 248 ():92-101.

Chicago/Turabian Style

Raquel Esteban; Idoia Ariz; Cristina Cruz; Jose Fernando Moran. 2016. "Review: Mechanisms of ammonium toxicity and the quest for tolerance." Plant Science 248, no. : 92-101.

Journal article
Published: 16 April 2016 in Journal of Experimental Botany
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A quantitative proteomic approach demonstrates how ammonium nutrition induces glucosinolate biosynthetic and catabolic pathways in Arabidopsis and broccoli.

ACS Style

Daniel Marino; Idoia Ariz; Berta Lasa; Enrique Santamaría; Joaquín Fernández-Irigoyen; Carmen Gonzalez-Murua; Pedro M. Aparicio Tejo. Quantitative proteomics reveals the importance of nitrogen source to control glucosinolate metabolism inArabidopsis thalianaandBrassica oleracea. Journal of Experimental Botany 2016, 67, 3313 -3323.

AMA Style

Daniel Marino, Idoia Ariz, Berta Lasa, Enrique Santamaría, Joaquín Fernández-Irigoyen, Carmen Gonzalez-Murua, Pedro M. Aparicio Tejo. Quantitative proteomics reveals the importance of nitrogen source to control glucosinolate metabolism inArabidopsis thalianaandBrassica oleracea. Journal of Experimental Botany. 2016; 67 (11):3313-3323.

Chicago/Turabian Style

Daniel Marino; Idoia Ariz; Berta Lasa; Enrique Santamaría; Joaquín Fernández-Irigoyen; Carmen Gonzalez-Murua; Pedro M. Aparicio Tejo. 2016. "Quantitative proteomics reveals the importance of nitrogen source to control glucosinolate metabolism inArabidopsis thalianaandBrassica oleracea." Journal of Experimental Botany 67, no. 11: 3313-3323.

Original research article
Published: 11 August 2015 in Frontiers in Plant Science
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The natural 15N/14N isotope composition (δ15N) of a tissue is a consequence of its N source and N physiological mechanisms in response to the environment. It could potentially be used as a tracer of N metabolism in plants under changing environmental conditions, where primary N metabolism may be complex, and losses and gains of N fluctuate over time. In order to test the utility of δ15N as an indicator of plant N status in N2-fixing plants grown under various environmental conditions, alfalfa (Medicago sativa L.) plants were subjected to distinct conditions of [CO2] (400 vs. 700 μmol mol−1), temperature (ambient vs. ambient +4°C) and water availability (fully watered vs. water deficiency—WD). As expected, increased [CO2] and temperature stimulated photosynthetic rates and plant growth, whereas these parameters were negatively affected by WD. The determination of δ15N in leaves, stems, roots, and nodules showed that leaves were the most representative organs of the plant response to increased [CO2] and WD. Depletion of heavier N isotopes in plants grown under higher [CO2] and WD conditions reflected decreased transpiration rates, but could also be related to a higher N demand in leaves, as suggested by the decreased leaf N and total soluble protein (TSP) contents detected at 700 μmol mol−1 [CO2] and WD conditions. In summary, leaf δ15N provides relevant information integrating parameters which condition plant responsiveness (e.g., photosynthesis, TSP, N demand, and water transpiration) to environmental conditions.

ACS Style

Idoia Eariz; Cristina Ecruz; Tome Eneves; Juan J. Irigoyen; Carmen Garcia-Olaverri; Salvador Enogués; Pedro M. Aparicio-Tejo; Iker Earanjuelo. Leaf δ15N as a physiological indicator of the responsiveness of N2-fixing alfalfa plants to elevated [CO2], temperature and low water availability. Frontiers in Plant Science 2015, 6, 574 .

AMA Style

Idoia Eariz, Cristina Ecruz, Tome Eneves, Juan J. Irigoyen, Carmen Garcia-Olaverri, Salvador Enogués, Pedro M. Aparicio-Tejo, Iker Earanjuelo. Leaf δ15N as a physiological indicator of the responsiveness of N2-fixing alfalfa plants to elevated [CO2], temperature and low water availability. Frontiers in Plant Science. 2015; 6 ():574.

Chicago/Turabian Style

Idoia Eariz; Cristina Ecruz; Tome Eneves; Juan J. Irigoyen; Carmen Garcia-Olaverri; Salvador Enogués; Pedro M. Aparicio-Tejo; Iker Earanjuelo. 2015. "Leaf δ15N as a physiological indicator of the responsiveness of N2-fixing alfalfa plants to elevated [CO2], temperature and low water availability." Frontiers in Plant Science 6, no. : 574.

Journal article
Published: 27 July 2013 in Plant and Soil
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Urea is the predominant form of N applied as fertilizer to crops, but it is also a significant N metabolite of plants themselves. As such, an understanding of urea metabolism in plants may contribute significantly to subsequent N fertilizer management. It currently appears that arginase is the only plant enzyme that can generate urea in vivo. The aim of this work was, therefore, to gain a more in-depth understanding of the significance of the inhibition of endogenous urease activity and its role in N metabolism depending on the N source supplied.

ACS Style

S. Cruchaga; B. Lasa; Iván Jauregui; Carmen Gonzalez-Murua; Pedro M Aparicio-Tejo; Idoia Ariz. Inhibition of endogenous urease activity by NBPT application reveals differential N metabolism responses to ammonium or nitrate nutrition in pea plants: a physiological study. Plant and Soil 2013, 373, 813 -827.

AMA Style

S. Cruchaga, B. Lasa, Iván Jauregui, Carmen Gonzalez-Murua, Pedro M Aparicio-Tejo, Idoia Ariz. Inhibition of endogenous urease activity by NBPT application reveals differential N metabolism responses to ammonium or nitrate nutrition in pea plants: a physiological study. Plant and Soil. 2013; 373 (1):813-827.

Chicago/Turabian Style

S. Cruchaga; B. Lasa; Iván Jauregui; Carmen Gonzalez-Murua; Pedro M Aparicio-Tejo; Idoia Ariz. 2013. "Inhibition of endogenous urease activity by NBPT application reveals differential N metabolism responses to ammonium or nitrate nutrition in pea plants: a physiological study." Plant and Soil 373, no. 1: 813-827.

Journal article
Published: 15 October 2012 in Physiologia Plantarum
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An understanding of the mechanisms underlying ammonium (NH4+) toxicity in plants requires prior knowledge of the metabolic uses for nitrogen (N) and carbon (C). We have recently shown that pea plants grown at high NH4+ concentrations suffer an energy deficiency associated with a disruption of ionic homeostasis. Furthermore, these plants are unable to adequately regulate internal NH4+ levels and the cell‐charge balance associated with cation uptake. Herein we show a role for an extra‐C application in the regulation of C–N metabolism in NH4+‐fed plants. Thus, pea plants (Pisum sativum) were grown at a range of NH4+ concentrations as sole N source, and two light intensities were applied to vary the C supply to the plants. Control plants grown at high NH4+ concentration triggered a toxicity response with the characteristic pattern of C‐starvation conditions. This toxicity response resulted in the redistribution of N from amino acids, mostly asparagine, and lower C/N ratios. The C/N imbalance at high NH4+ concentration under control conditions induced a strong activation of root C metabolism and the upregulation of anaplerotic enzymes to provide C intermediates for the tricarboxylic acid cycle. A high light intensity partially reverted these C‐starvation symptoms by providing higher C availability to the plants. The extra‐C contributed to a lower C4/C5 amino acid ratio while maintaining the relative contents of some minor amino acids involved in key pathways regulating the C/N status of the plants unchanged. C availability can therefore be considered to be a determinant factor in the tolerance/sensitivity mechanisms to NH4+ nutrition in plants.

ACS Style

Idoia Ariz; Aaron C. Asensio; Angel M. Zamarreño; Jose M. García‐Mina; Pedro M. Aparicio-Tejo; Jose Fernando Moran. Changes in the C/N balance caused by increasing external ammonium concentrations are driven by carbon and energy availabilities during ammonium nutrition in pea plants: the key roles of asparagine synthetase and anaplerotic enzymes. Physiologia Plantarum 2012, 148, 522 -537.

AMA Style

Idoia Ariz, Aaron C. Asensio, Angel M. Zamarreño, Jose M. García‐Mina, Pedro M. Aparicio-Tejo, Jose Fernando Moran. Changes in the C/N balance caused by increasing external ammonium concentrations are driven by carbon and energy availabilities during ammonium nutrition in pea plants: the key roles of asparagine synthetase and anaplerotic enzymes. Physiologia Plantarum. 2012; 148 (4):522-537.

Chicago/Turabian Style

Idoia Ariz; Aaron C. Asensio; Angel M. Zamarreño; Jose M. García‐Mina; Pedro M. Aparicio-Tejo; Jose Fernando Moran. 2012. "Changes in the C/N balance caused by increasing external ammonium concentrations are driven by carbon and energy availabilities during ammonium nutrition in pea plants: the key roles of asparagine synthetase and anaplerotic enzymes." Physiologia Plantarum 148, no. 4: 522-537.

Journal article
Published: 01 May 2012 in Journal of Plant Physiology
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The development of new nitrogen fertilizers is necessary to optimize crop production whilst improving the environmental aspects arising from the use of nitrogenous fertilization as a cultural practice. The use of urease inhibitors aims to improve the efficiency of urea as a nitrogen fertilizer by preventing its loss from the soil as ammonia. However, although the action of urease inhibitors is aimed at the urease activity in soil, their availability for the plant may affect its urease activity. The aim of this work was therefore to evaluate the effect of two urease inhibitors, namely acetohydroxamic acid (AHA) and N-(n-butyl) thiophosphoric triamide (NBPT), on the germination of pea and spinach seeds. The results obtained show that urease inhibitors do not affect the germination process to any significant degree, with the only process affected being imbibition in spinach, thus also suggesting different urease activities for both plants. Our findings therefore suggest an activity other than the previously reported urolytic activity for urease in spinach. Furthermore, of the two inhibitors tested, NBPT was found to be the most effective at inhibiting urease activity, especially in pea seedlings.

ACS Style

Idoia Ariz; Saioa Cruchaga; Berta Lasa; Jose Fernando Moran; Ivan Jauregui; Pedro M. Aparicio-Tejo. The physiological implications of urease inhibitors on N metabolism during germination of Pisum sativum and Spinacea oleracea seeds. Journal of Plant Physiology 2012, 169, 673 -681.

AMA Style

Idoia Ariz, Saioa Cruchaga, Berta Lasa, Jose Fernando Moran, Ivan Jauregui, Pedro M. Aparicio-Tejo. The physiological implications of urease inhibitors on N metabolism during germination of Pisum sativum and Spinacea oleracea seeds. Journal of Plant Physiology. 2012; 169 (7):673-681.

Chicago/Turabian Style

Idoia Ariz; Saioa Cruchaga; Berta Lasa; Jose Fernando Moran; Ivan Jauregui; Pedro M. Aparicio-Tejo. 2012. "The physiological implications of urease inhibitors on N metabolism during germination of Pisum sativum and Spinacea oleracea seeds." Journal of Plant Physiology 169, no. 7: 673-681.

Journal article
Published: 19 February 2012 in Plant Growth Regulation
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A careful control of the N nutritional status of grapevines can have a determining effect on wine characteristics; therefore a suitable management of N fertilization might allow some wine parameters to be modified, thereby improving product quality. The aim of this study was to determine the effect of foliar application of urea at different doses and different times of the growing season on the parameters of Sauvignon Blanc and Merlot grape juice. The research described herein involved Sauvignon Blanc and Merlot grapevines (V. vinifera L.) at a commercial vineyard and was conducted over 2 years. In the first year, N treatment involved a foliar application at a dose of 10 kg N ha−1 during veraison, whereas in the second year it involved a foliar urea application at two doses (10 and 50 kg N ha−1) and at three different times—3 weeks before veraison, during veraison and 3 weeks after veraison. In this second year, the urea applied at a dose of 10 kg N ha−1 was isotopically labelled with 1% 15N. Chemical parameters, yeast assimilable N, amino acid content, amino acid profile and N isotopic composition were determined for all treatments. Grape and grape-juice parameters for Merlot were found to be more affected by N fertilization than for Sauvignon Blanc and were also more affected during the second year than during the first year, thus indicating that the climatic characteristics of each campaign could affect these parameters. The yeast assimilable N in grape juice was found to be higher for late applications of foliar urea, with application of the higher dose of urea during veraison increasing the amino acid and proline contents in both varieties. The isotopic analysis data showed that the urea applied to leaves was transferred to the berries, with the maximum translocation in Sauvignon Blanc occurring for the post-veraison treatment and in Merlot for the veraison treatment. We can therefore conclude that foliar application of urea could modify grape juice quality and could therefore be used as a tool for obtaining quality wines.

ACS Style

Berta Lasa; Sergio Menéndez; Kepa Sagastizabal; Maria Erendira Calleja-Cervantes; Ignacio Irigoyen; Julio Muro; Pedro M Aparicio-Tejo; Idoia Ariz. Foliar application of urea to “Sauvignon Blanc” and “Merlot” vines: doses and time of application. Plant Growth Regulation 2012, 67, 73 -81.

AMA Style

Berta Lasa, Sergio Menéndez, Kepa Sagastizabal, Maria Erendira Calleja-Cervantes, Ignacio Irigoyen, Julio Muro, Pedro M Aparicio-Tejo, Idoia Ariz. Foliar application of urea to “Sauvignon Blanc” and “Merlot” vines: doses and time of application. Plant Growth Regulation. 2012; 67 (1):73-81.

Chicago/Turabian Style

Berta Lasa; Sergio Menéndez; Kepa Sagastizabal; Maria Erendira Calleja-Cervantes; Ignacio Irigoyen; Julio Muro; Pedro M Aparicio-Tejo; Idoia Ariz. 2012. "Foliar application of urea to “Sauvignon Blanc” and “Merlot” vines: doses and time of application." Plant Growth Regulation 67, no. 1: 73-81.

Journal article
Published: 01 October 2011 in Molecular Plant-Microbe Interactions®
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Two phylogenetically unrelated superoxide dismutase (SOD) families, i.e., CuZnSOD (copper and zinc SOD) and FeMn-CamSOD (iron, manganese, or cambialistic SOD), eliminate superoxide radicals in different locations within the plant cell. CuZnSOD are located within the cytosol and plastids, while the second family of SOD, which are considered to be of bacterial origin, are usually located within organelles, such as mitochondria. We have used the reactive oxygen species–producer methylviologen (MV) to study SOD isozymes in the indeterminate nodules on pea (Pisum sativum). MV caused severe effects on nodule physiology and structure and also resulted in an increase in SOD activity. Purification and N-terminal analysis identified CamSOD from the Rhizobium leguminosarum endosymbiont as one of the most active SOD in response to the oxidative stress. Fractionation of cell extracts and immunogold labeling confirmed that the CamSOD was present in both the bacteroids and the cytosol (including the nuclei, plastids, and mitochondria) of the N-fixing cells, and also within the uninfected cortical and interstitial cells. These findings, together with previous reports of the occurrence of FeSOD in determinate nodules, indicate that FeMnCamSOD have specific functions in legumes, some of which may be related to signaling between plant and bacterial symbionts, but the occurrence of one or more particular isozymes depends upon the nodule type.

ACS Style

Aaron C. Asensio; Daniel Marino; Euan James; Idoia Ariz; Cesar Arrese-Igor; Pedro M Aparicio-Tejo; Raul Arredondo-Peter; Jose Fernando Moran. Expression and Localization of a Rhizobium-Derived Cambialistic Superoxide Dismutase in Pea (Pisum sativum) Nodules Subjected to Oxidative Stress. Molecular Plant-Microbe Interactions® 2011, 24, 1247 -1257.

AMA Style

Aaron C. Asensio, Daniel Marino, Euan James, Idoia Ariz, Cesar Arrese-Igor, Pedro M Aparicio-Tejo, Raul Arredondo-Peter, Jose Fernando Moran. Expression and Localization of a Rhizobium-Derived Cambialistic Superoxide Dismutase in Pea (Pisum sativum) Nodules Subjected to Oxidative Stress. Molecular Plant-Microbe Interactions®. 2011; 24 (10):1247-1257.

Chicago/Turabian Style

Aaron C. Asensio; Daniel Marino; Euan James; Idoia Ariz; Cesar Arrese-Igor; Pedro M Aparicio-Tejo; Raul Arredondo-Peter; Jose Fernando Moran. 2011. "Expression and Localization of a Rhizobium-Derived Cambialistic Superoxide Dismutase in Pea (Pisum sativum) Nodules Subjected to Oxidative Stress." Molecular Plant-Microbe Interactions® 24, no. 10: 1247-1257.

Journal article
Published: 01 July 2011 in Journal of Plant Physiology
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The widespread use of NO3− fertilization has had a major ecological impact. NH4+ nutrition may help to reduce this impact, although high NH4+ concentrations are toxic for most plants. The underlying tolerance mechanisms are not yet fully understood, although they are thought to include the limitation of C, the disruption of ion homeostasis, and a wasteful NH4+ influx/efflux cycle that carries an extra energetic cost for root cells. In this study, high irradiance (HI) was found to induce a notable tolerance to NH4+ in the range 2.5–10 mM in pea plants by inducing higher C availability, as shown by carbohydrate content. This capacity was accompanied by a general lower relative N content, indicating that tolerance is not achieved through higher net N assimilation on C-skeletons, and it was also not attributable to increased GS content or activity in roots or leaves. Moreover, HI plants showed higher ATP content and respiration rates. This extra energy availability is related to the internal NH4+ content regulation (probably NH4+ influx/efflux) and to an improvement of the cell ionic balance. The limited C availability at lower irradiance (LI) and high NH4+ resulted in a series of metabolic imbalances, as reflected in a much higher organic acid content, thereby suggesting that the origin of the toxicity in plants cultured at high NH4+ and LI is related to their inability to avoid large-scale accumulation of the NH4+ ion.

ACS Style

Idoia Ariz; Ekhiñe Artola; Aaron Cabrera Asensio; Saioa Cruchaga; Pedro María Aparicio-Tejo; Jose Fernando Moran. High irradiance increases NH4+ tolerance in Pisum sativum: Higher carbon and energy availability improve ion balance but not N assimilation. Journal of Plant Physiology 2011, 168, 1009 -1015.

AMA Style

Idoia Ariz, Ekhiñe Artola, Aaron Cabrera Asensio, Saioa Cruchaga, Pedro María Aparicio-Tejo, Jose Fernando Moran. High irradiance increases NH4+ tolerance in Pisum sativum: Higher carbon and energy availability improve ion balance but not N assimilation. Journal of Plant Physiology. 2011; 168 (10):1009-1015.

Chicago/Turabian Style

Idoia Ariz; Ekhiñe Artola; Aaron Cabrera Asensio; Saioa Cruchaga; Pedro María Aparicio-Tejo; Jose Fernando Moran. 2011. "High irradiance increases NH4+ tolerance in Pisum sativum: Higher carbon and energy availability improve ion balance but not N assimilation." Journal of Plant Physiology 168, no. 10: 1009-1015.

Journal article
Published: 01 March 2011 in Journal of Plant Physiology
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The application of urease inhibitors in conjunction with urea fertilizers as a means of reducing N loss due to ammonia volatilization requires an in-depth study of the physiological effects of these inhibitors on plants. The aim of this study was to determine how the urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) affects N metabolism in pea and spinach. Plants were cultivated in pure hydroponic culture with urea as the sole N source. After 2 weeks of growth for pea, and 3 weeks for spinach, half of the plants received NBPT in their nutrient solution. Urease activity, urea and ammonium content, free amino acid composition and soluble protein were determined in leaves and roots at days 0, 1, 2, 4, 7 and 9, and the NBPT content in these tissues was determined 48 h after inhibitor application. The results suggest that the effects of NBPT on spinach and pea urease activity differ, with pea being most affected by this treatment, and that the NBPT absorbed by the plant caused a clear inhibition of the urease activity in pea leaf and roots. The high urea concentration observed in leaves was associated with the development of necrotic leaf margins, and was further evidence of NBPT inhibition in these plants. A decrease in the ammonium content in roots, where N assimilation mainly takes place, was also observed. Consequently, total amino acid contents were drastically reduced upon NBPT treatment, indicating a strong alteration of the N metabolism. Furthermore, the amino acid profile showed that amidic amino acids were major components of the reduced pool of amino acids. In contrast, NBPT was absorbed to a much lesser degree by spinach plants than pea plants (35% less) and did not produce a clear inhibition of urease activity in this species.

ACS Style

Saioa Cruchaga; Ekhiñe Artola; Berta Lasa; Idoia Ariz; Ignacio Irigoyen; Jose Fernando Moran; Pedro M. Aparicio-Tejo. Short term physiological implications of NBPT application on the N metabolism of Pisum sativum and Spinacea oleracea. Journal of Plant Physiology 2011, 168, 329 -336.

AMA Style

Saioa Cruchaga, Ekhiñe Artola, Berta Lasa, Idoia Ariz, Ignacio Irigoyen, Jose Fernando Moran, Pedro M. Aparicio-Tejo. Short term physiological implications of NBPT application on the N metabolism of Pisum sativum and Spinacea oleracea. Journal of Plant Physiology. 2011; 168 (4):329-336.

Chicago/Turabian Style

Saioa Cruchaga; Ekhiñe Artola; Berta Lasa; Idoia Ariz; Ignacio Irigoyen; Jose Fernando Moran; Pedro M. Aparicio-Tejo. 2011. "Short term physiological implications of NBPT application on the N metabolism of Pisum sativum and Spinacea oleracea." Journal of Plant Physiology 168, no. 4: 329-336.

Journal article
Published: 01 January 2011 in BMC Plant Biology
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In plants, nitrate (NO3 -) nutrition gives rise to a natural N isotopic signature (δ15N), which correlates with the δ15N of the N source. However, little is known about the relationship between the δ15N of the N source and the 14N/15N fractionation in plants under ammonium (NH4 +) nutrition. When NH4 + is the major N source, the two forms, NH4 + and NH3, are present in the nutrient solution. There is a 1.025 thermodynamic isotope effect between NH3 (g) and NH4 + (aq) which drives to a different δ15N. Nine plant species with different NH4 +-sensitivities were cultured hydroponically with NO3 - or NH4 + as the sole N sources, and plant growth and δ15N were determined. Short-term NH4 +/NH3 uptake experiments at pH 6.0 and 9.0 (which favours NH3 form) were carried out in order to support and substantiate our hypothesis. N source fractionation throughout the whole plant was interpreted on the basis of the relative transport of NH4 + and NH3.

ACS Style

Idoia Ariz; Cristina Cruz; Jose F. Moran; Gonzalez Moro; Carmen García-Olaverri; Carmen González-Murua; Maria A. Martins-Loução; Pedro M Aparicio-Tejo. Depletion of the heaviest stable N isotope is associated with NH4+/NH3 toxicity in NH4+-fed plants. BMC Plant Biology 2011, 11, 83 -83.

AMA Style

Idoia Ariz, Cristina Cruz, Jose F. Moran, Gonzalez Moro, Carmen García-Olaverri, Carmen González-Murua, Maria A. Martins-Loução, Pedro M Aparicio-Tejo. Depletion of the heaviest stable N isotope is associated with NH4+/NH3 toxicity in NH4+-fed plants. BMC Plant Biology. 2011; 11 (1):83-83.

Chicago/Turabian Style

Idoia Ariz; Cristina Cruz; Jose F. Moran; Gonzalez Moro; Carmen García-Olaverri; Carmen González-Murua; Maria A. Martins-Loução; Pedro M Aparicio-Tejo. 2011. "Depletion of the heaviest stable N isotope is associated with NH4+/NH3 toxicity in NH4+-fed plants." BMC Plant Biology 11, no. 1: 83-83.

Journal article
Published: 01 September 2010 in Journal of Plant Physiology
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8 p., 7 figures and bibliographyPhotosynthesis provides plant metabolism with reduced carbon (C) but is also the main source of oxidative stress in plants. Likewise, high doses of NH4+ as sole N source have been reported to be toxic for most plants, resulting in reduced plant growth and restricting C availability. The combination of high photosynthetic photon flux densities (PPFD) and NH4+ nutrition may provide higher C availability but could also have a detrimental effect on the plants, therefore the objective of this study is to evaluate whether NH4+ induces photo-oxidative stress that is exacerbated under high light conditions. Pea plants (Pisum sativum cv. sugar-snap) were grown hydroponically with NH4+ (0.5, 2.5, 5 and 10mM) under high (750μmolphotonsm -2s -1) or low PPFD conditions (350μmolphotonsm -2s -1). High PPFD contributes to a higher tolerance to ammonium by pea plants, as it originated higher biomass content due to higher photosynthetic rates. However, a deficit of N (0.5 and 2.5mM NH4+) under high PPFD conditions caused an antioxidant response, as indicated by increased photoprotective pigment and chloroplastic superoxide dismutase contents. Plants grown with higher doses of N and high PPFD showed less need for photoprotection. An increase in the specific leaf weight (SLW) ratio was observed associated not only with high PPFDs but also with the highest NH4+ dose. Overall, these results demonstrate that, despite the activation of some photoprotective responses at high PPFD, there were no photoinhibitory symptoms and a positive effect on NH4+ toxicity, thus suggesting that the harmful effects of NH 4 + are not directly related to the generation of photo-oxidative stress.This work was supported by the Spanish MICIIN (grant nos. AGL2006-12792-CO2-01 and AGL2003-06571-CO2-01 [to P.A.-T.] and AGL2007-64432/AGR [to J.F.M.]), by the Government of Navarra (Res 57/2007 to J.F.M.), and BFU 2007-62637 of MEC and\ud EHU-GV IT-299-07 of the Basque Government to JMB and JIG-P. IA was supported by a doctoral Fellowship from the Public University of Navarre, Spain.Peer reviewe

ACS Style

Idoia Ariz; Raquel Esteban; José Ignacio García-Plazaola; Jose Maria Becerril; Pedro María Aparicio-Tejo; Jose Fernando Moran. High irradiance induces photoprotective mechanisms and a positive effect on NH4+ stress in Pisum sativum L. Journal of Plant Physiology 2010, 167, 1038 -1045.

AMA Style

Idoia Ariz, Raquel Esteban, José Ignacio García-Plazaola, Jose Maria Becerril, Pedro María Aparicio-Tejo, Jose Fernando Moran. High irradiance induces photoprotective mechanisms and a positive effect on NH4+ stress in Pisum sativum L. Journal of Plant Physiology. 2010; 167 (13):1038-1045.

Chicago/Turabian Style

Idoia Ariz; Raquel Esteban; José Ignacio García-Plazaola; Jose Maria Becerril; Pedro María Aparicio-Tejo; Jose Fernando Moran. 2010. "High irradiance induces photoprotective mechanisms and a positive effect on NH4+ stress in Pisum sativum L." Journal of Plant Physiology 167, no. 13: 1038-1045.

Journal article
Published: 25 August 2010 in Plant Growth Regulation
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The use of urea as an N fertilizer has increased to such an extent that it is now the most widely used fertilizer in the world. However, N losses as a result of ammonia volatilization lead to a decrease in its efficiency, therefore different methods have been developed over the years to reduce these losses. One of the most recent involves the use of urea combined with urease inhibitors, such as N-(n-butyl) thiophosphoric triamide (NBPT), in an attempt to delay the hydrolysis of urea in the soil. The aim of this study was to perform an in-depth analysis of the effect that NBPT use has on plant growth and N metabolism. Wheat plants were cultivated in a greenhouse experiment lasting 4 weeks and fertilized with urea and NBPT at different concentrations (0, 0.012, 0.062, 0.125%). Each treatment was replicated six times. A non-fertilized control was also cultivated. Several parameters related with N metabolism were analysed at the end of growth period. NBPT use was found to have visible effects, such as a transitory yellowing of the leaf tips, at the end of the first week of treatment. At a metabolic level, plants treated with the inhibitor were found to have more urea in their tissues and a lower amino acid content, lower glutamine synthetase activity, and lower urease and glutamine synthetase content at the end of the study period, whereas their urease activity seemed to have recovered by this stageThis work was supported by the Spanish MICIIN (grant no. AGL2009-13339-CO2-02 [to P.A.T.]). S.C was supported by a doctoral fellowship from the Public University of Navarre.Peer Reviewe

ACS Style

Ekhiñe Artola; Saioa Cruchaga; Idoia Ariz; Jose Fernando Moran; María Garnica; Fabrice Houdusse; Jose Maria Garcia-Mina; Ignacio Irigoyen; Berta Lasa; Pedro María Aparicio-Tejo. Effect of N-(n-butyl) thiophosphoric triamide on urea metabolism and the assimilation of ammonium by Triticum aestivum L. Plant Growth Regulation 2010, 63, 73 -79.

AMA Style

Ekhiñe Artola, Saioa Cruchaga, Idoia Ariz, Jose Fernando Moran, María Garnica, Fabrice Houdusse, Jose Maria Garcia-Mina, Ignacio Irigoyen, Berta Lasa, Pedro María Aparicio-Tejo. Effect of N-(n-butyl) thiophosphoric triamide on urea metabolism and the assimilation of ammonium by Triticum aestivum L. Plant Growth Regulation. 2010; 63 (1):73-79.

Chicago/Turabian Style

Ekhiñe Artola; Saioa Cruchaga; Idoia Ariz; Jose Fernando Moran; María Garnica; Fabrice Houdusse; Jose Maria Garcia-Mina; Ignacio Irigoyen; Berta Lasa; Pedro María Aparicio-Tejo. 2010. "Effect of N-(n-butyl) thiophosphoric triamide on urea metabolism and the assimilation of ammonium by Triticum aestivum L." Plant Growth Regulation 63, no. 1: 73-79.

Book chapter
Published: 01 January 2008 in Methods in Enzymology
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Ferric leghemoglobin reductase (FLbR) is able to reduce ferric leghemoglobin (Lb3+) to ferrous (Lb2+) form. This reaction makes Lb functional in performing its role since only reduced hemoglobins bind O2. FLbR contains FAD as prosthetic group to perform its activity. FLbR-1 and FLbR-2 were isolated from soybean root nodules and it has been postulated that they reduce Lb3+. The existence of Lb2+ is essential for the nitrogen fixation process that occurs in legume nodules; thus, the isolation of FLbR for the study of this enzyme in the nodule physiology is of interest. However, previous methods for the production of recombinant FLbR are inefficient as yields are too low. We describe the production of a recombinant FLbR-2 from Escherichia coli BL21(DE3) by using an overexpression method based on the self-induction of the recombinant E. coli. This expression system is four times more efficient than the previous overexpression method. The quality of recombinant FLbR-2 (based on spectroscopy, SDS-PAGE, IEF, and native PAGE) is comparable to that of the previous expression system. Also, FLbR-2 is purified near to homogeneity in only few steps (in a time scale, the full process takes 3 days). The purification method involves affinity chromatography using a Ni-nitrilotriacetic acid column. Resulting rFLbR-2 showed an intense yellow color, and spectral characterization of rFLbR-2 indicated that rFLbR-2 contains flavin. Pure rFLbR-2 was incubated with soybean Lba and NADH, and time drive rates showed that rFLbR-2 efficiently reduces Lb3+.

ACS Style

Estibaliz Urarte; Iñigo Auzmendi; Selene Rol; Idoia Ariz; Pedro M Aparicio-Tejo; Raúl Arredondo‐Peter; Jose Fernando Moran. A Self‐Induction Method to Produce High Quantities of Recombinant Functional Flavo‐Leghemoglobin Reductase. Methods in Enzymology 2008, 436, 411 -423.

AMA Style

Estibaliz Urarte, Iñigo Auzmendi, Selene Rol, Idoia Ariz, Pedro M Aparicio-Tejo, Raúl Arredondo‐Peter, Jose Fernando Moran. A Self‐Induction Method to Produce High Quantities of Recombinant Functional Flavo‐Leghemoglobin Reductase. Methods in Enzymology. 2008; 436 ():411-423.

Chicago/Turabian Style

Estibaliz Urarte; Iñigo Auzmendi; Selene Rol; Idoia Ariz; Pedro M Aparicio-Tejo; Raúl Arredondo‐Peter; Jose Fernando Moran. 2008. "A Self‐Induction Method to Produce High Quantities of Recombinant Functional Flavo‐Leghemoglobin Reductase." Methods in Enzymology 436, no. : 411-423.

Review
Published: 01 January 2008 in Methods in Enzymology
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Superoxide dismutases (SODs; EC 1.15.1.1) are a group of metalloenzymes which are essential to protect cells under aerobic conditions. In biological systems, it has been reported that SODs and other proteins are susceptible to be attacked by peroxynitrite (ONOO(-)) which can be originated from the reaction of nitric oxide with superoxide radical. ONOO(-) is a strong oxidant molecule capable of nitrating peptides and proteins at the phenyl side chain of the tyrosine residues. In the present work, bovine serum albumin (BSA) and recombinant iron-superoxide dismutase from the plant cowpea (Vu_FeSOD) are used as target molecules to estimate ONOO(-) production. The method employs the compound SIN-1, which simultaneously generates *NO and O(2)(-) in aerobic aqueous solutions. First, assay conditions were optimized incubating BSA with different concentrations of SIN-1, and at a later stage, the effect on the tyrosine nitration and catalytic activity of Vu_FeSOD was examined by in-gel activity and spectrophotometric assays. Both BSA and Vu_FeSOD are nitrated in a dose-dependent manner, and, at least in BSA nitration, the reaction seems to be metal catalyzed.

ACS Style

Estibaliz Larrainzar; Estibaliz Urarte; Iñigo Auzmendi; Idoia Ariz; Cesar Arrese‐Igor; Esther M. González; Jose Fernando Moran. Use of Recombinant Iron‐Superoxide Dismutase as A Marker of Nitrative Stress. Methods in Enzymology 2008, 437, 605 -618.

AMA Style

Estibaliz Larrainzar, Estibaliz Urarte, Iñigo Auzmendi, Idoia Ariz, Cesar Arrese‐Igor, Esther M. González, Jose Fernando Moran. Use of Recombinant Iron‐Superoxide Dismutase as A Marker of Nitrative Stress. Methods in Enzymology. 2008; 437 ():605-618.

Chicago/Turabian Style

Estibaliz Larrainzar; Estibaliz Urarte; Iñigo Auzmendi; Idoia Ariz; Cesar Arrese‐Igor; Esther M. González; Jose Fernando Moran. 2008. "Use of Recombinant Iron‐Superoxide Dismutase as A Marker of Nitrative Stress." Methods in Enzymology 437, no. : 605-618.