The effects of temperature and moisture on flexural and thermomechanical properties of neat and filled epoxy with both multiwall carbon nanotubes (CNT), carbon nanofibers (CNF), and their hybrid components were investigated. Two regimes of environmental aging were applied: Water absorption at 70 °C until equilibrium moisture content and thermal heating at 70 °C for the same time period. Three-point bending and dynamic mechanical tests were carried out for all samples before and after conditioning. The property prediction model (PPM) was successfully applied for the prediction of the modulus of elasticity in bending of manufactured specimens subjected to both water absorption and thermal aging. It was experimentally confirmed that, due to addition of carbon nanofillers to the epoxy resin, the sorption, flexural, and thermomechanical characteristics were slightly improved compared to the neat system. Considering experimental and theoretical results, most of the epoxy composites filled with hybrid carbon nanofiller revealed the lowest effect of temperature and moisture on material properties, along with the lowest sorption characteristics.

The hypothesis of the present research is that by altering the substrate topography and/or stiffness to make it biomimetic, we can modulate cells behavior. Substrates with similar surface chemistry and varying stiffnesses and topographies were prepared. Bulk PCL and CNTs-reinforced PCL composites were manufactured by solvent casting method and electrospinning and further processed to obtain tunable moduli of elasticity in the range of few MPa. To ensure the same chemical profile for the substrates, a protein coating was added. Substrate topography and properties were investigated. Further on, the feedback of Wharton’s Jelly Umbilical Cord Mesenchymal Stem Cells to substrates characteristics was investigated. Solvent casting scaffolds displayed superior mechanical properties compared to the corresponding electrospun films. However, the biomimetic fibrous texture of the electrospun substrates induced improved feedback of the cells with respect to their viability and proliferation. Cells’ adhesion and differentiation was remarkably pronounced on solvent casting substrates compared to the electrospun substrates. Soft substates improved cells multiplication and migration, while stiff substrates induced differentiation into bone cells. Aspects related to the key factors and the ideal properties of substrates and microenvironments were clarified, aiming towards the deep understanding of the required optimum biomimetic features of biomaterials.

Autologous cell therapy uses patients’ own cells to deliver precise and ideal treatment through a personalized medicine approach. Isolation of patients’ cells from residual tissue extracted during surgery involves specific planning and lab steps. In the present manuscript, a path from isolation to in vitro research with human mesenchymal stem cells (MSCs) obtained from residual bone tissues is described as performed by a medical unit in collaboration with a research center. Ethical issues have been addressed by formulating appropriate harvesting protocols according to European regulations. Samples were collected from 19 patients; 10 of them were viable and after processing resulted in MSCs. MSCs were further differentiated in osteoblasts to investigate the biocompatibility of several 3D scaffolds produced by electrospinning and 3D printing technologies; traditional orthopedic titanium and nanostructured titanium substrates were also tested. 3D printed scaffolds proved superior compared to other substrates, enabling significantly improved response in osteoblast cells, indicating that their biomimetic structure and properties make them suitable for synthetic tissue engineering. The present research is a proof of concept that describes the process of primary stem cells isolation for in vitro research and opens avenues for the development of personalized cell platforms in the case of patients with orthopedic trauma. The demonstration model has promising perspectives in personalized medicine practices.

The present study focuses on the mechanical and viscoelastic characterization of 3D-printed PLA, fabricated in three different printing orientations (0°, 45°, and 90°) and four different nozzle temperatures (210, 220, 230, and 240 °C). By employing a combination of static and dynamic mechanical analysis (DMA) testing, as well as differential scanning calorimetry (DSC) analysis, this work aims to investigate the relationship between processing parameters and the resulting properties of PLA. DSC results showed that higher nozzle temperatures enhance the degree of crystallinity, which in turn affects the mechanical and viscoelastic behavior of PLA. Regardless of the nozzle temperature, the flexural strength decreased as the printing orientation degrees increased. However, it was found that the higher the nozzle temperature, the higher the flexural strength for the same orientation, and the smaller the strength deviations per specimen. DMA results indicated that as the printing orientation increased, glass transition temperature (T_g) values increased while storage modulus values decreased. At the same time, in both cases, by increasing nozzle temperature, an increase in T_g and a respective increase in storage modulus values is observed due to the increase in the degree of crystallinity.

The goal of the present investigation was to find a solution to crucial engineering aspects related to the elaboration of multi-layered tissue-biomimicking composites. 3D printing technology was used to manufacture single-layered and gradient multi-layered 3D porous scaffolds made of poly-lactic acid (PLA). The scaffolds manufacturing process was optimized after adjusting key printing parameters. The scaffolds with 60 μm side length (square-shaped pores) showed increased stiffness values comparing to the other specimens. A silicone adhesive has been further used to join biomedical titanium plates, and the PLA scaffolds; in addition, titania nanotubes (TNTs were produced on the titanium for improved adhesion. The titanium-PLA scaffold single lap joints were evaluated in micro-tensile testing. The electrochemical processing of the titanium surface resulted in a 248% increase of the ultimate strength in the overlap area for dry specimens and 40% increase for specimens immersed in simulated body fluid. Finally, the biocompatibility of the produced scaffolds was evaluated with primary cell populations obtained after isolation from bone residual tissue. The manufactured scaffolds present promising features for applications in orthopedic implantology and are worth further.

The response of fiber-reinforced polymer composites to an externally applied mechanical excitation is closely related to the microscopic stress transfer mechanisms taking place in the fiber–matrix interphasial region. In particular, in the case of viscoelastic responses, these mechanisms are time dependent. Defining the interphase thickness as the maximum radial distance from the fiber surface where a specific matrix property is affected by the fiber presence, it is important to study its variation with time. In the present investigation, the stress relaxation behavior of a glass fiber-reinforced polymer (GFRP) under flexural conditions was studied. Next, applying the hybrid viscoelastic interphase model (HVIM), developed by the first author, the interphase modulus and interphase thickness were both evaluated, and their variation with time during the stress relaxation test was plotted. It was found that the interphase modulus decreases with the radial distance, being always higher than the bulk matrix modulus. In addition, the interphase thickness increases with time, showing that during stress relaxation, fiber–matrix debonding takes place. Finally, the effect of fiber interaction on the interphase modulus was found. It is observed that fiber interaction depends on both the fiber–matrix degree of adhesion as well as the fiber volume fraction and the time-dependent interphase modulus.