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Associate Professor Noppol Leksawasdi, PhD received the Royal Thai Government Scholarship) (Ministry of Science and Technology) to undertake his Bachelor and Doctorate Degrees at The University of New South Wales (UNSW), Sydney, Australia on Bioprocess Engineering / Biotechnology between 1994 – 2004 during which he earned several awards such as The Outstanding Student (Science Stream), Cargill Australia Prize, and The University Medal. He currently has been awarded up to 87 research grants, 74 publications in the national / international refereed journals, 1 national patent, 2 pending national patents, and 26 copyrighted computer programs designed in Visual Basic for Applications (VBA) for Microsoft® EXCEL. He was tenured by the School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Thailand since 2004 and previously served as a Head, School of Agro-Industry between 2011 – 2019 as well as the Director of Bioprocess Research Cluster (BRC) since 2014 and Deputy Director, Office of Research Administration (ORA), University Office, Chiang Mai University (2019 – 2020). His current position is the Director of ORA since 2020. His other past experiences on the administrative positions includes Assistant Dean (2005 – 2009) and Associate Dean on Research, Academic Services and Foreign Affairs (2009 - 2011).
Polylactic acid (PLA) was melt-blended with epoxy resin to study the effects of the reaction on the mechanical and thermal properties of the PLA. The addition of 0.5% (wt/wt) epoxy to PLA increased the maximum tensile strength of PLA (57.5 MPa) to 67 MPa, whereas the 20% epoxy improved the elongation at break to 12%, due to crosslinking caused by the epoxy reaction. The morphology of the PLA/epoxy blends showed epoxy nanoparticle dispersion in the PLA matrix that presented a smooth fracture surface with a high epoxy content. The glass transition temperature of PLA decreased with an increasing epoxy content owing to the partial miscibility between PLA and the epoxy resin. The Vicat softening temperature of the PLA was 59 °C and increased to 64.6 °C for 0.5% epoxy. NMR confirmed the reaction between the -COOH groups of PLA and the epoxy groups of the epoxy resin. This reaction, and partial miscibility of the PLA/epoxy blend, improved the interfacial crosslinking, morphology, thermal properties, and mechanical properties of the blends.
Krittameth Kiattipornpithak; Nanthicha Thajai; Thidarat Kanthiya; Pornchai Rachtanapun; Noppol Leksawasdi; Yuthana Phimolsiripol; David Rohindra; Warintorn Ruksiriwanich; Sarana Rose Sommano; Kittisak Jantanasakulwong. Reaction Mechanism and Mechanical Property Improvement of Poly(Lactic Acid) Reactive Blending with Epoxy Resin. Polymers 2021, 13, 2429 .
AMA StyleKrittameth Kiattipornpithak, Nanthicha Thajai, Thidarat Kanthiya, Pornchai Rachtanapun, Noppol Leksawasdi, Yuthana Phimolsiripol, David Rohindra, Warintorn Ruksiriwanich, Sarana Rose Sommano, Kittisak Jantanasakulwong. Reaction Mechanism and Mechanical Property Improvement of Poly(Lactic Acid) Reactive Blending with Epoxy Resin. Polymers. 2021; 13 (15):2429.
Chicago/Turabian StyleKrittameth Kiattipornpithak; Nanthicha Thajai; Thidarat Kanthiya; Pornchai Rachtanapun; Noppol Leksawasdi; Yuthana Phimolsiripol; David Rohindra; Warintorn Ruksiriwanich; Sarana Rose Sommano; Kittisak Jantanasakulwong. 2021. "Reaction Mechanism and Mechanical Property Improvement of Poly(Lactic Acid) Reactive Blending with Epoxy Resin." Polymers 13, no. 15: 2429.
The (R)-phenylacetylcarbinol (PAC) batch biotransformation kinetics for partially purified Candida tropicalis TISTR 5350 pyruvate decarboxylase (PDC) were determined to validate a comprehensive mathematical model in 250 mL scale with 250 mM phosphate buffer/pH 7.0. PDC could convert initial 100/120 mM benzaldehyde/pyruvate substrates to the statistical significantly highest (p ≤ 0.05) maximum PAC concentration (95.8 ± 0.1 mM) and production rate (0.639 ± 0.001 mM min−1). A parameter search strategy aimed at minimizing overall residual sum of square (RSS T ) based on a system of six ordinary differential equations was applied to PAC biotransformation profiles with initial benzaldehyde/pyruvate concentration of 100/120 and 30/36 mM. Ten important biotransformation kinetic parameters were then elucidated including the zeroth order activation rate constant due to phosphate buffer species (k a ) of (9.38 ± < 0.01) × 10–6% relative PDC activity min−1 mM−1. The validation of this model to independent biotransformation kinetics with initial benzaldehyde/pyruvate concentration of 50/60 mM resulted in relatively good fitting with RSS T , mean sum of square error (MSE), and coefficient of determination (R2) values of 662, 17.4, and 0.9863, respectively.
Julaluk Khemacheewakul; Siraphat Taesuwan; Rojarej Nunta; Charin Techapun; Yuthana Phimolsiripol; Pornchai Rachtanapun; Kittisak Jantanasakulwong; Kritsadaporn Porninta; Sumeth Sommanee; Chatchadaporn Mahakuntha; Thanongsak Chaiyaso; Phisit Seesuriyachan; Alissara Reungsang; Ngoc Thao Ngan Trinh; Sutee Wangtueai; Sarana Rose Sommano; Noppol Leksawasdi. Validation of mathematical model with phosphate activation effect by batch (R)-phenylacetylcarbinol biotransformation process utilizing Candida tropicalis pyruvate decarboxylase in phosphate buffer. Scientific Reports 2021, 11, 1 -11.
AMA StyleJulaluk Khemacheewakul, Siraphat Taesuwan, Rojarej Nunta, Charin Techapun, Yuthana Phimolsiripol, Pornchai Rachtanapun, Kittisak Jantanasakulwong, Kritsadaporn Porninta, Sumeth Sommanee, Chatchadaporn Mahakuntha, Thanongsak Chaiyaso, Phisit Seesuriyachan, Alissara Reungsang, Ngoc Thao Ngan Trinh, Sutee Wangtueai, Sarana Rose Sommano, Noppol Leksawasdi. Validation of mathematical model with phosphate activation effect by batch (R)-phenylacetylcarbinol biotransformation process utilizing Candida tropicalis pyruvate decarboxylase in phosphate buffer. Scientific Reports. 2021; 11 (1):1-11.
Chicago/Turabian StyleJulaluk Khemacheewakul; Siraphat Taesuwan; Rojarej Nunta; Charin Techapun; Yuthana Phimolsiripol; Pornchai Rachtanapun; Kittisak Jantanasakulwong; Kritsadaporn Porninta; Sumeth Sommanee; Chatchadaporn Mahakuntha; Thanongsak Chaiyaso; Phisit Seesuriyachan; Alissara Reungsang; Ngoc Thao Ngan Trinh; Sutee Wangtueai; Sarana Rose Sommano; Noppol Leksawasdi. 2021. "Validation of mathematical model with phosphate activation effect by batch (R)-phenylacetylcarbinol biotransformation process utilizing Candida tropicalis pyruvate decarboxylase in phosphate buffer." Scientific Reports 11, no. 1: 1-11.
The effect of ultrasonication on the antioxidant and antibacterial properties of Ceylon spinach (Basella alba) extracts (CE) and the shelf life of chilled pork with CE were studied. The CE were ultrasonicated at different power levels (60–100%) for 10–40 min in an ultrasonic bath with the rise of antioxidant activities (p ≤ 0.05) proportional to the ultrasonication time. The additional investigation of antibacterial activities showed that the ultrasonicated extracts (100 mg/mL) could inhibit and inactivate Staphylococcus aureus and Escherichia coli with the optimal condition of 80% power for 40 min. For shelf life testing, fresh pork treated with the ultrasonicated extracts at 100 and 120 mg/mL had lower values of thiobarbituric acid reactive substances (TBARS) than the control (without dipping). For food safety as measured by the total microbial count, the fresh pork dipped with 100–120 mg/mL CE extract could be kept at 0 °C for 7 days, 2 to 3 days longer than control meat at 0 and 4 °C, respectively. A sensory evaluation using a nine-point hedonic scale showed that fresh pork dipped with 100-mg/mL CE extracts was accepted by consumers. It is suggested that CE extracts can be applied in the food industry to enhance the quality and extend the shelf life of meat products.
Yuthana Phimolsiripol; Srirana Buadoktoom; Pimporn Leelapornpisid; Kittisak Jantanasakulwong; Phisit Seesuriyachan; Thanongsak Chaiyaso; Noppol Leksawasdi; Pornchai Rachtanapun; Nareekan Chaiwong; Sarana Sommano; Charles Brennan; Joe Regenstein. Shelf Life Extension of Chilled Pork by Optimal Ultrasonicated Ceylon Spinach (Basella alba) Extracts: Physicochemical and Microbial Properties. Foods 2021, 10, 1241 .
AMA StyleYuthana Phimolsiripol, Srirana Buadoktoom, Pimporn Leelapornpisid, Kittisak Jantanasakulwong, Phisit Seesuriyachan, Thanongsak Chaiyaso, Noppol Leksawasdi, Pornchai Rachtanapun, Nareekan Chaiwong, Sarana Sommano, Charles Brennan, Joe Regenstein. Shelf Life Extension of Chilled Pork by Optimal Ultrasonicated Ceylon Spinach (Basella alba) Extracts: Physicochemical and Microbial Properties. Foods. 2021; 10 (6):1241.
Chicago/Turabian StyleYuthana Phimolsiripol; Srirana Buadoktoom; Pimporn Leelapornpisid; Kittisak Jantanasakulwong; Phisit Seesuriyachan; Thanongsak Chaiyaso; Noppol Leksawasdi; Pornchai Rachtanapun; Nareekan Chaiwong; Sarana Sommano; Charles Brennan; Joe Regenstein. 2021. "Shelf Life Extension of Chilled Pork by Optimal Ultrasonicated Ceylon Spinach (Basella alba) Extracts: Physicochemical and Microbial Properties." Foods 10, no. 6: 1241.
The new (R) - phenylacetylcarbinol (PAC) batch biotransformation kinetics for partially purified Candida tropicalis TISTR 5350 pyruvate decarboxylase (PDC) were characterized and validated with a comprehensive mathematical model in 250 mL scale with 250 mM phosphate buffer / pH 7.0. PDC could convert initial 100 / 120 mM benzaldehyde / pyruvate substrates to the statistical significantly highest (p £ 0.05) maximum PAC concentration (95.8 ± 0.1 mM) and production rate (0.639 ± 0.001 mM min-1). A parameter search strategy aimed at minimizing overall residual sum of square (RSS T ) based on a system of six ordinary differential equations was applied to PAC biotransformation profiles with initial benzaldehyde / pyruvate concentration of 100/120 and 30/36 mM. Ten important biotransformation kinetic parameters were then elucidated including the zeroth order activation rate constant due to phosphate buffer species (k a ) of (9.38 ± < 0.01) × 10-6 % relative PDC activity min-1 mM-1. The validation of this model to independent biotransformation kinetics with initial benzaldehyde / pyruvate concentration of 50 / 60 mM resulted in relatively good fitting with RSS T , mean sum of square error (MSE), and coefficient of determination (R2) values of 662, 17.4, and 0.9863, respectively.Novelty: The existing (R) - phenylacetylcarbinol (PAC) biotransformation model in literatures did not include activation effect of buffering species into the enzyme deactivating rate equation, thereby background deactivation and buffering activation effects could not be discerned. A new mathematical model which incorporated activation effect, using 250 mM phosphate buffer as an example, was able to predict and validate PAC batch biotransformation systems reasonably well and could pave the way for model applications in more complex processes.
Julaluk Khemacheewakul; Siraphat Taesuwan; Rojarej Nunta; Charin Techapun; Yuthana Phimolsiripol; Pornchai Rachtanapun; Kittisak Jantanasakulwong; Kritsadaporn Porninta; Sumeth Sommanee; Chatchadaporn Mahakuntha; Thanongsak Chaiyaso; Phisit Seesuriyachan; Alissara Reungsang; Ngoc Thao Ngan Trinh; Sutee Wangtueai; Sarana Rose Sommano; Noppol Leksawasdi. Mathematical Model With Phosphate Activation Effect Validates Batch (R)-phenylacetylcarbinol Biotransformation Process Utilizing Candida Tropicalis Pyruvate Decarboxylase in Phosphate Buffer . 2021, 1 .
AMA StyleJulaluk Khemacheewakul, Siraphat Taesuwan, Rojarej Nunta, Charin Techapun, Yuthana Phimolsiripol, Pornchai Rachtanapun, Kittisak Jantanasakulwong, Kritsadaporn Porninta, Sumeth Sommanee, Chatchadaporn Mahakuntha, Thanongsak Chaiyaso, Phisit Seesuriyachan, Alissara Reungsang, Ngoc Thao Ngan Trinh, Sutee Wangtueai, Sarana Rose Sommano, Noppol Leksawasdi. Mathematical Model With Phosphate Activation Effect Validates Batch (R)-phenylacetylcarbinol Biotransformation Process Utilizing Candida Tropicalis Pyruvate Decarboxylase in Phosphate Buffer . . 2021; ():1.
Chicago/Turabian StyleJulaluk Khemacheewakul; Siraphat Taesuwan; Rojarej Nunta; Charin Techapun; Yuthana Phimolsiripol; Pornchai Rachtanapun; Kittisak Jantanasakulwong; Kritsadaporn Porninta; Sumeth Sommanee; Chatchadaporn Mahakuntha; Thanongsak Chaiyaso; Phisit Seesuriyachan; Alissara Reungsang; Ngoc Thao Ngan Trinh; Sutee Wangtueai; Sarana Rose Sommano; Noppol Leksawasdi. 2021. "Mathematical Model With Phosphate Activation Effect Validates Batch (R)-phenylacetylcarbinol Biotransformation Process Utilizing Candida Tropicalis Pyruvate Decarboxylase in Phosphate Buffer ." , no. : 1.
Curcumin is a phenolic compound derived from turmeric roots (Curcuma longa L.). This research studied the effects of curcumin extract on the properties of chitosan films. The film characteristics measured included mechanical properties, visual aspects, color parameters, light transmission, moisture content, water solubility, water vapor permeability, infrared spectroscopy, and antioxidant activity. The results suggest that adding curcumin to chitosan-based films increases yellowness and light barriers. Infrared spectroscopy analysis showed interactions between the phenolic compounds of the extract and the chitosan, which may have improved the mechanical properties and reduced the moisture content, water solubility, and water vapor permeability of the films. The antioxidant activity of the films increased with increasing concentrations of the curcumin extract. This study shows the potential benefits of incorporating curcumin extract into chitosan films used as active packaging.
Pornchai Rachtanapun; Warinporn Klunklin; Pensak Jantrawut; Kittisak Jantanasakulwong; Yuthana Phimolsiripol; Phisit Seesuriyachan; Noppol Leksawasdi; Thanongsak Chaiyaso; Warintorn Ruksiriwanich; Suphat Phongthai; Sarana Sommano; Winita Punyodom; Alissara Reungsang; Thi Ngo. Characterization of Chitosan Film Incorporated with Curcumin Extract. Polymers 2021, 13, 963 .
AMA StylePornchai Rachtanapun, Warinporn Klunklin, Pensak Jantrawut, Kittisak Jantanasakulwong, Yuthana Phimolsiripol, Phisit Seesuriyachan, Noppol Leksawasdi, Thanongsak Chaiyaso, Warintorn Ruksiriwanich, Suphat Phongthai, Sarana Sommano, Winita Punyodom, Alissara Reungsang, Thi Ngo. Characterization of Chitosan Film Incorporated with Curcumin Extract. Polymers. 2021; 13 (6):963.
Chicago/Turabian StylePornchai Rachtanapun; Warinporn Klunklin; Pensak Jantrawut; Kittisak Jantanasakulwong; Yuthana Phimolsiripol; Phisit Seesuriyachan; Noppol Leksawasdi; Thanongsak Chaiyaso; Warintorn Ruksiriwanich; Suphat Phongthai; Sarana Sommano; Winita Punyodom; Alissara Reungsang; Thi Ngo. 2021. "Characterization of Chitosan Film Incorporated with Curcumin Extract." Polymers 13, no. 6: 963.
Pectin recovered from mango peel biomass can be used as a potential source for pectic oligosaccharide hydrolysate with excellent probiotic growth-enhancing performance and prebiotic potentials. Consequently, the objectives of the current study were to optimise the enzyme hydrolysis treatment of mango peel pectin (MPP) and to evaluate the pectic oligosaccharide effects of Lactobacillus reuteri DSM 17938 and Bifidobacterium animalis TISTR 2195. Mango of “chok anan” variety was chosen due to its excessive volume of biomass in processing and high pectin content. The optimal treatment for mango peel pectic oligosaccharide (MPOS) valorisation was 24 h of fermentation with 0.3% (v/v) pectinase. This condition provided small oligosaccharides with the molecular weight of 643 Da that demonstrated the highest score of prebiotic activity for both of B. animalis TISTR 2195 (7.76) and L. reuteri DSM 17938 (6.87). The major sugar compositions of the oligosaccharide were fructose (24.41% (w/w)) and glucose (19.52% (w/w)). For the simulation of prebiotic fermentation, B. animalis TISTR 2195 showed higher proliferation in 4% (w/v) of MPOS supplemented (8.92 log CFU/mL) than that of L. reuteri (8.53 CFU/mL) at 72 h of the fermentation time. The main short chain fatty acids (SCFAs) derived from MPOS were acetic acid and propionic acid. The highest value of total SCFA was achieved from the 4% (w/v) MPOS supplementation for both of B. animalis (68.57 mM) and L. reuteri (69.15 mM). The result of this study therefore conclusively advises that MPOS is a novel pectic oligosaccharide resource providing the opportunity for the sustainable development approach through utilising by-products from the fruit industry.
Malaiporn Wongkaew; Bow Tinpovong; Korawan Sringarm; Noppol Leksawasdi; Kittisak Jantanasakulwong; Pornchai Rachtanapun; Prasert Hanmoungjai; Sarana Sommano. Crude Pectic Oligosaccharide Recovery from Thai Chok Anan Mango Peel Using Pectinolytic Enzyme Hydrolysis. Foods 2021, 10, 627 .
AMA StyleMalaiporn Wongkaew, Bow Tinpovong, Korawan Sringarm, Noppol Leksawasdi, Kittisak Jantanasakulwong, Pornchai Rachtanapun, Prasert Hanmoungjai, Sarana Sommano. Crude Pectic Oligosaccharide Recovery from Thai Chok Anan Mango Peel Using Pectinolytic Enzyme Hydrolysis. Foods. 2021; 10 (3):627.
Chicago/Turabian StyleMalaiporn Wongkaew; Bow Tinpovong; Korawan Sringarm; Noppol Leksawasdi; Kittisak Jantanasakulwong; Pornchai Rachtanapun; Prasert Hanmoungjai; Sarana Sommano. 2021. "Crude Pectic Oligosaccharide Recovery from Thai Chok Anan Mango Peel Using Pectinolytic Enzyme Hydrolysis." Foods 10, no. 3: 627.
Nata de coco has been used as a raw material for food preparation. In this study, the production of carboxymethyl cellulose (CMC) film from nata de coco and the effect of monochloroacetic acid on carboxymethyl bacterial cellulose (CMC n ) and its film were investigated. Bacterial cellulose from nata de coco was modified into CMC form via carboxymethylation using various concentrations of monochloroacetic acid (MCA) at 6, 12, 18, and 24 g per 15 g of cellulose. The results showed that different concentrations of MCA affected the degree of substitution (DS), chemical structure, viscosity, color, crystallinity, and morphology of CMC n . The optimum treatment for carboxymethylation was found using 24 g of MCA per 15 g of cellulose, which provided the highest DS at 0.83. The morphology of CMC n was related to DS value; a higher DS value showed denser and smoother surface than nata de coco cellulose. The various MCA concentrations increased the mechanical properties (tensile strength and percentage of elongation at break) and water vapor permeability of CMC n , which were related to the DS value.
Pornchai Rachtanapun; Warinporn Klunklin; Pensak Jantrawut; Noppol Leksawasdi; Kittisak Jantanasakulwong; Yuthana Phimolsiripol; Phisit Seesuriyachan; Thanongsak Chaiyaso; Warintorn Ruksiriwanich; Suphat Phongthai; Sarana Sommano; Winita Punyodom; Alissara Reungsang; Thi Ngo. Effect of Monochloroacetic Acid on Properties of Carboxymethyl Bacterial Cellulose Powder and Film from Nata de Coco. Polymers 2021, 13, 488 .
AMA StylePornchai Rachtanapun, Warinporn Klunklin, Pensak Jantrawut, Noppol Leksawasdi, Kittisak Jantanasakulwong, Yuthana Phimolsiripol, Phisit Seesuriyachan, Thanongsak Chaiyaso, Warintorn Ruksiriwanich, Suphat Phongthai, Sarana Sommano, Winita Punyodom, Alissara Reungsang, Thi Ngo. Effect of Monochloroacetic Acid on Properties of Carboxymethyl Bacterial Cellulose Powder and Film from Nata de Coco. Polymers. 2021; 13 (4):488.
Chicago/Turabian StylePornchai Rachtanapun; Warinporn Klunklin; Pensak Jantrawut; Noppol Leksawasdi; Kittisak Jantanasakulwong; Yuthana Phimolsiripol; Phisit Seesuriyachan; Thanongsak Chaiyaso; Warintorn Ruksiriwanich; Suphat Phongthai; Sarana Sommano; Winita Punyodom; Alissara Reungsang; Thi Ngo. 2021. "Effect of Monochloroacetic Acid on Properties of Carboxymethyl Bacterial Cellulose Powder and Film from Nata de Coco." Polymers 13, no. 4: 488.
Bacterial cellulose from nata de coco was prepared from the fermentation of coconut juice with Acetobacter xylinum for 10 days at room temperature under sterile conditions. Carboxymethyl cellulose (CMC) was transformed from the bacterial cellulose from the nata de coco by carboxymethylation using different concentrations of sodium hydroxide (NaOH) and monochloroacetic acid (MCA) in an isopropyl (IPA) medium. The effects of various NaOH concentrations on the degree of substitution (DS), chemical structure, viscosity, color, crystallinity, morphology and the thermal properties of carboxymethyl bacterial cellulose powder from nata de coco (CMCn) were evaluated. In the carboxymethylation process, the optimal condition resulted from NaOH amount of 30 g/100 mL, as this provided the highest DS value (0.92). The crystallinity of CMCn declined after synthesis but seemed to be the same in each condition. The mechanical properties (tensile strength and percentage of elongation at break), water vapor permeability (WVP) and morphology of CMCn films obtained from CMCn synthesis using different NaOH concentrations were investigated. The tensile strength of CMCn film synthesized with a NaOH concentration of 30 g/100 mL increased, however it declined when the amount of NaOH concentration was too high. This result correlated with the DS value. The highest percent elongation at break was obtained from CMCn films synthesized with 50 g/100 mL NaOH, whereas the elongation at break decreased when NaOH concentration increased to 60 g/100 mL.
Pornchai Rachtanapun; Pensak Jantrawut; Warinporn Klunklin; Kittisak Jantanasakulwong; Yuthana Phimolsiripol; Noppol Leksawasdi; Phisit Seesuriyachan; Thanongsak Chaiyaso; Chayatip Insomphun; Suphat Phongthai; Sarana Sommano; Winita Punyodom; Alissara Reungsang; Thi Ngo. Carboxymethyl Bacterial Cellulose from Nata de Coco: Effects of NaOH. Polymers 2021, 13, 348 .
AMA StylePornchai Rachtanapun, Pensak Jantrawut, Warinporn Klunklin, Kittisak Jantanasakulwong, Yuthana Phimolsiripol, Noppol Leksawasdi, Phisit Seesuriyachan, Thanongsak Chaiyaso, Chayatip Insomphun, Suphat Phongthai, Sarana Sommano, Winita Punyodom, Alissara Reungsang, Thi Ngo. Carboxymethyl Bacterial Cellulose from Nata de Coco: Effects of NaOH. Polymers. 2021; 13 (3):348.
Chicago/Turabian StylePornchai Rachtanapun; Pensak Jantrawut; Warinporn Klunklin; Kittisak Jantanasakulwong; Yuthana Phimolsiripol; Noppol Leksawasdi; Phisit Seesuriyachan; Thanongsak Chaiyaso; Chayatip Insomphun; Suphat Phongthai; Sarana Sommano; Winita Punyodom; Alissara Reungsang; Thi Ngo. 2021. "Carboxymethyl Bacterial Cellulose from Nata de Coco: Effects of NaOH." Polymers 13, no. 3: 348.
Cellulose from Asparagus officinalis stalk end was extracted and synthesized to carboxymethyl cellulose (CMCas) using monochloroacetic acid (MCA) via carboxymethylation reaction with various sodium hydroxide (NaOH) concentrations starting from 20% to 60%. The cellulose and CMCas were characterized by the physical properties, Fourier Transform Infrared spectroscopy (FTIR), Differential scanning calorimetry (DSC), Scanning electron microscopy (SEM) and X-ray diffraction (XRD). In addition, mechanical properties of CMCas films were also investigated. The optimum condition for producing CMCas was found to be 30% of NaOH concentration for the carboxymethylation reaction, which provided the highest percent yield of CMCas at 44.04% with the highest degree of substitution (DS) at 0.98. The melting point of CMCas decreased with increasing NaOH concentrations. Crystallinity of CMCas was significantly deformed (p < 0.05) after synthesis at a high concentration. The L* value of the CMCas was significantly lower at a high NaOH concentration compared to the cellulose. The highest tensile strength (44.59 MPa) was found in CMCas film synthesized with 40% of NaOH concentration and the highest percent elongation at break (24.99%) was obtained in CMCas film treated with 30% of NaOH concentration. The applications of asparagus stalk end are as biomaterials in drug delivery system, tissue engineering, coating, and food packaging.
Warinporn Klunklin; Kittisak Jantanasakulwong; Yuthana Phimolsiripol; Noppol Leksawasdi; Phisit Seesuriyachan; Thanongsak Chaiyaso; Chayatip Insomphun; Suphat Phongthai; Pensak Jantrawut; Sarana Rose Sommano; Winita Punyodom; Alissara Reungsang; Thi Minh Phuong Ngo; Pornchai Rachtanapun. Synthesis, Characterization, and Application of Carboxymethyl Cellulose from Asparagus Stalk End. Polymers 2020, 13, 81 .
AMA StyleWarinporn Klunklin, Kittisak Jantanasakulwong, Yuthana Phimolsiripol, Noppol Leksawasdi, Phisit Seesuriyachan, Thanongsak Chaiyaso, Chayatip Insomphun, Suphat Phongthai, Pensak Jantrawut, Sarana Rose Sommano, Winita Punyodom, Alissara Reungsang, Thi Minh Phuong Ngo, Pornchai Rachtanapun. Synthesis, Characterization, and Application of Carboxymethyl Cellulose from Asparagus Stalk End. Polymers. 2020; 13 (1):81.
Chicago/Turabian StyleWarinporn Klunklin; Kittisak Jantanasakulwong; Yuthana Phimolsiripol; Noppol Leksawasdi; Phisit Seesuriyachan; Thanongsak Chaiyaso; Chayatip Insomphun; Suphat Phongthai; Pensak Jantrawut; Sarana Rose Sommano; Winita Punyodom; Alissara Reungsang; Thi Minh Phuong Ngo; Pornchai Rachtanapun. 2020. "Synthesis, Characterization, and Application of Carboxymethyl Cellulose from Asparagus Stalk End." Polymers 13, no. 1: 81.
Bioresource evaluation is prerequisite and important to reduce cost of feedstock collection and avoid battle for feedstock to promote the healthy development of bioenergy industry. This study estimated Thai bioresources from arable field crops, horticultural plants and livestock with product quantity or livestock number, residue-product ratio or manure productivity, and moisture content. Rice straw and husk, para rubber residues and cattle manure separately have the top amount in arable field crop biomass, horticultural residues and livestock manures. The northeastern region has the most amounts of arable field crop biomass and livestock manures, and the southern region possesses the largest quantities of horticultural residues. The available energy potentials from residues of arable field crops and horticultural plants can reach to maximum of 4.91 × 105 TJ and 7.65 × 105 TJ, respectively, which can theoretically share 21.67% of current total primary energy supply. The available biogas potential from livestock manures is nearly ten times than its current generation. After analysis the status of technologies and government policies for Thai bioenergy industry, it indicates that the utilization of bioenergy in the form of electricity, heat and transportation fuels has promising prospect in Thailand. The provinces of Thailand which are more suitable for developing bioenergy industry are suggested. This work may guide the reasonable layout of bioenergy industry in Thailand via the presence of bioresouces distribution in every province.
Wen Wang; Kritsadaporn Porninta; Pruk Aggarangsi; Noppol Leksawasdi; Lianhua Li; Xiaoyan Chen; Xinshu Zhuang; Zhenhong Yuan; Wei Qi. Bioenergy development in Thailand based on the potential estimation from crop residues and livestock manures. Biomass and Bioenergy 2020, 144, 105914 .
AMA StyleWen Wang, Kritsadaporn Porninta, Pruk Aggarangsi, Noppol Leksawasdi, Lianhua Li, Xiaoyan Chen, Xinshu Zhuang, Zhenhong Yuan, Wei Qi. Bioenergy development in Thailand based on the potential estimation from crop residues and livestock manures. Biomass and Bioenergy. 2020; 144 ():105914.
Chicago/Turabian StyleWen Wang; Kritsadaporn Porninta; Pruk Aggarangsi; Noppol Leksawasdi; Lianhua Li; Xiaoyan Chen; Xinshu Zhuang; Zhenhong Yuan; Wei Qi. 2020. "Bioenergy development in Thailand based on the potential estimation from crop residues and livestock manures." Biomass and Bioenergy 144, no. : 105914.
The aim of this work was to synthesize carboxymethyl cellulose (CMC) and produce CMC films from the cellulose of palm bunch and bagasse agricultural waste. The effect of various amounts of H2O2 (0–40% v/v) during delignification on the properties of cellulose, CMC, and CMC films was studied. As the H2O2 content increased, yield and the lignin content of the cellulose from palm bunch and bagasse decreased, whereas lightness (L*) and whiteness index (WI) increased. FTIR confirmed the substitution of a carboxymethyl group on the cellulose structure. A higher degree of substitution of CMC from both sources was found when 20%–30% H2O2 was employed. The trend in the L* and WI values of each CMC and CMC film was related to those values in their respective cellulose. Bleaching each cellulose with 20% H2O2 provided the cellulose with the highest viscosity and the CMC films with the greatest mechanical (higher tensile strength and elongation at break) and soluble attributes, but the lowest water vapor barrier. This evidence indicates that cellulose delignification with H2O2 has a strong effect on the appearance and physical properties of both CMCs.
Rungsiri Suriyatem; Nichaya Noikang; Tamolwan Kankam; Kittisak Jantanasakulwong; Noppol Leksawasdi; Yuthana Phimolsiripol; Chayatip Insomphun; Phisit Seesuriyachan; Thanongsak Chaiyaso; Pensak Jantrawut; Sarana Rose Sommano; Thi Minh Phuong Ngo; Pornchai Rachtanapun. Physical Properties of Carboxymethyl Cellulose from Palm Bunch and Bagasse Agricultural Wastes: Effect of Delignification with Hydrogen Peroxide. Polymers 2020, 12, 1505 .
AMA StyleRungsiri Suriyatem, Nichaya Noikang, Tamolwan Kankam, Kittisak Jantanasakulwong, Noppol Leksawasdi, Yuthana Phimolsiripol, Chayatip Insomphun, Phisit Seesuriyachan, Thanongsak Chaiyaso, Pensak Jantrawut, Sarana Rose Sommano, Thi Minh Phuong Ngo, Pornchai Rachtanapun. Physical Properties of Carboxymethyl Cellulose from Palm Bunch and Bagasse Agricultural Wastes: Effect of Delignification with Hydrogen Peroxide. Polymers. 2020; 12 (7):1505.
Chicago/Turabian StyleRungsiri Suriyatem; Nichaya Noikang; Tamolwan Kankam; Kittisak Jantanasakulwong; Noppol Leksawasdi; Yuthana Phimolsiripol; Chayatip Insomphun; Phisit Seesuriyachan; Thanongsak Chaiyaso; Pensak Jantrawut; Sarana Rose Sommano; Thi Minh Phuong Ngo; Pornchai Rachtanapun. 2020. "Physical Properties of Carboxymethyl Cellulose from Palm Bunch and Bagasse Agricultural Wastes: Effect of Delignification with Hydrogen Peroxide." Polymers 12, no. 7: 1505.
This research aimed to synthesize carboxymethyl chitosan (CMCH) from different molecular weights of chitosan including low MW (L, 50–190 kDa), medium MW (M, 210–300 kDa) and high MW (H, 310–375 kDa) on the antioxidant and moisturizing properties. The L-CMCH, M-CMCH and H-CMCH improved the water solubility by about 96%, 90% and 89%, respectively when compared to native chitosan. Higher MW resulted in more viscous of CMCH. For antioxidant properties, IC50 values of DPPH and ABTS radical scavenging activity for L-CMCH were 1.70 and 1.37 mg/mL, respectively. The L-CMCH had higher antioxidant properties by DPPH and ABTS radical scavenging assay and FRAP. The moisturizing properties on pig skin using a Corneometer® showed that 0.5% H-CMCH significantly presented (p ≤ 0.05) greater moisturizing effect than that of untreated-skin, distilled water, propylene glycol and pure chitosan from three molecular weights.
Nareekan Chaiwong; Pimporn Leelapornpisid; Kittisak Jantanasakulwong; Pornchai Rachtanapun; Phisit Seesuriyachan; Vinyoo Sakdatorn; Noppol Leksawasdi; Yuthana Phimolsiripol. Antioxidant and Moisturizing Properties of Carboxymethyl Chitosan with Different Molecular Weights. Polymers 2020, 12, 1445 .
AMA StyleNareekan Chaiwong, Pimporn Leelapornpisid, Kittisak Jantanasakulwong, Pornchai Rachtanapun, Phisit Seesuriyachan, Vinyoo Sakdatorn, Noppol Leksawasdi, Yuthana Phimolsiripol. Antioxidant and Moisturizing Properties of Carboxymethyl Chitosan with Different Molecular Weights. Polymers. 2020; 12 (7):1445.
Chicago/Turabian StyleNareekan Chaiwong; Pimporn Leelapornpisid; Kittisak Jantanasakulwong; Pornchai Rachtanapun; Phisit Seesuriyachan; Vinyoo Sakdatorn; Noppol Leksawasdi; Yuthana Phimolsiripol. 2020. "Antioxidant and Moisturizing Properties of Carboxymethyl Chitosan with Different Molecular Weights." Polymers 12, no. 7: 1445.
Thermoplastic starch (TPS) was prepared by melt-mixing cassava starch with glycerol. Polyethylene (PE), polyethylene-grafted-maleic anhydride (PE-MAH) and poly(lactic acid) (PLA) solutions at 2% (w/v) were used to coat TPS using the dip coating process. The tensile strength of TPS increased with the dip coating solution technique, especially for PLA coating. Swelling index, water-soluble matter and water droplet contact angle confirmed the water resistant improvement of TPS by PE-MAH and the PLA dip coating solution. Plasticizer bleeding was found in uncoated TPS after storage, but not in the coated TPS. Coating TPS with PE-MAH and PLA improved the tensile properties, water resistance and conquered plasticizer bleeding problems in TPS.
Kittisak Jantanasakulwong; Nattagarn Homsaard; Phanurot Phengchan; Pornchai Rachtanapun; Noppol Leksawasdi; Yuthana Phimolsiripol; Charin Techapun; Pensak Jantrawut. Effect of Dip Coating Polymer Solutions on Properties of Thermoplastic Cassava Starch. Polymers 2019, 11, 1746 .
AMA StyleKittisak Jantanasakulwong, Nattagarn Homsaard, Phanurot Phengchan, Pornchai Rachtanapun, Noppol Leksawasdi, Yuthana Phimolsiripol, Charin Techapun, Pensak Jantrawut. Effect of Dip Coating Polymer Solutions on Properties of Thermoplastic Cassava Starch. Polymers. 2019; 11 (11):1746.
Chicago/Turabian StyleKittisak Jantanasakulwong; Nattagarn Homsaard; Phanurot Phengchan; Pornchai Rachtanapun; Noppol Leksawasdi; Yuthana Phimolsiripol; Charin Techapun; Pensak Jantrawut. 2019. "Effect of Dip Coating Polymer Solutions on Properties of Thermoplastic Cassava Starch." Polymers 11, no. 11: 1746.
Rojarej Nunta; Charin Techapun; Kittisak Jantanasakulwong; Thanongsak Chaiyaso; Phisit Seesuriyachan; Julaluk Khemacheewakul; Chatchadaporn Mahakuntha; Kritsadaporn Porninta; Sumeth Sommanee; Ngoc T. Trinh; Noppol Leksawasdi. Batch and continuous cultivation processes ofCandida tropicalisTISTR 5306 for ethanol and pyruvate decarboxylase production in fresh longan juice with optimal carbon to nitrogen molar ratio. Journal of Food Process Engineering 2019, 42, 1 .
AMA StyleRojarej Nunta, Charin Techapun, Kittisak Jantanasakulwong, Thanongsak Chaiyaso, Phisit Seesuriyachan, Julaluk Khemacheewakul, Chatchadaporn Mahakuntha, Kritsadaporn Porninta, Sumeth Sommanee, Ngoc T. Trinh, Noppol Leksawasdi. Batch and continuous cultivation processes ofCandida tropicalisTISTR 5306 for ethanol and pyruvate decarboxylase production in fresh longan juice with optimal carbon to nitrogen molar ratio. Journal of Food Process Engineering. 2019; 42 (6):1.
Chicago/Turabian StyleRojarej Nunta; Charin Techapun; Kittisak Jantanasakulwong; Thanongsak Chaiyaso; Phisit Seesuriyachan; Julaluk Khemacheewakul; Chatchadaporn Mahakuntha; Kritsadaporn Porninta; Sumeth Sommanee; Ngoc T. Trinh; Noppol Leksawasdi. 2019. "Batch and continuous cultivation processes ofCandida tropicalisTISTR 5306 for ethanol and pyruvate decarboxylase production in fresh longan juice with optimal carbon to nitrogen molar ratio." Journal of Food Process Engineering 42, no. 6: 1.
The filamentous microalgae Tribonema minus accumulates large amounts of lipids under photoautotrophic condition, while under heterotrophic condition, the lipid content decreased dramatically. Determination of the differences in metabolic pathways between photoautotrophic and heterotrophic growth will provide targets and strategies for improvement of lipid accumulation in heterotrophic cells. The metabolic differences between photoautotrophically and heterotrophically cultivated T. minus cells were studied by comparing the growth, biochemical compositions and transcriptomic and metabolomic profiles of the cells. Based on comparative transcriptomic and metabolomic studies, we generated a global model of the changes in central carbon metabolism and lipid biosynthetic pathways that occur under photoautotrophic and heterotrophic growth conditions. Moreover, the specific effects of supplementation with exogenous key metabolic intermediates on the lipid accumulation in heterotrophic culture were analyzed. Compared to photoautotrophic cultures, heterotrophic cultures exhibited enhanced biomass levels and carbohydrate content, but decreased lipid accumulation. These effects were accompanied by low expression levels of genes involved in glycolysis, de novo fatty acids biosynthesis and lipid biosynthesis, and high levels of genes involved in gluconeogenesis. In addition, the levels of key metabolites involved in glycolysis/gluconeogenesis were elevated in abundance, whereas those of certain fatty acids and citric acid were decreased in heterotrophic cultures. Upon supplementation with exogenous potassium palmitate, the lipid content increased dramatically in heterotrophically cultivated T. minus. An insufficient supply of carbon precursors caused the low levels of lipid accumulation during heterotrophic cultivation. Appropriate carbon metabolite supplementation based on the metabolomic data was shown to promote lipid accumulation. Moreover, gene regulatory metabolic targets were also identified via omics analysis.
Hui Wang; Yan Zhang; Wenjun Zhou; Leksawasdi Noppol; Tianzhong Liu. Mechanism and enhancement of lipid accumulation in filamentous oleaginous microalgae Tribonema minus under heterotrophic condition. Biotechnology for Biofuels 2018, 11, 1 -14.
AMA StyleHui Wang, Yan Zhang, Wenjun Zhou, Leksawasdi Noppol, Tianzhong Liu. Mechanism and enhancement of lipid accumulation in filamentous oleaginous microalgae Tribonema minus under heterotrophic condition. Biotechnology for Biofuels. 2018; 11 (1):1-14.
Chicago/Turabian StyleHui Wang; Yan Zhang; Wenjun Zhou; Leksawasdi Noppol; Tianzhong Liu. 2018. "Mechanism and enhancement of lipid accumulation in filamentous oleaginous microalgae Tribonema minus under heterotrophic condition." Biotechnology for Biofuels 11, no. 1: 1-14.
Fresh juice from C grade longan (mg/g fresh fruit; total sugars 51.6 ± 0.5, nitrogen 0.021 ± 0.004; gallic acid 0.025 ± 0.001; ellagic acid 0.016 ± 0.001) with addition of 8.52 g ammonium sulfate/l was the optimal medium for cultivation processes of Saccharomyces cerevisiae TISTR 5606 and Candida tropicalis TISTR 5306 capable of producing ethanol and phenylacetylcarbinol (PAC) among five longan grades. S. cerevisiae TISTR 5606 produced ethanol (33.4 ± 3.2 g/L) at a significantly higher level (p ≤ 0.05) than C. tropicalis TISTR 5306 (22.3 ± 1.1 g/L) with similar ethanol yields (Yp/s) between 0.21 and 0.22 g ethanol produced/g sugars consumed. Whole cells of C. tropicalis TISTR 5306 produced a significantly higher (p ≤ 0.05) PAC level (27.2 ± 0.7 mM) than S. cerevisiae TISTR 5606 (3.59 ± 1.33 mM) after 6 hr in an equivalent volume biphasic biotransformation system. Practical applications Longan is one of the important economic fruit of Thailand with production volume in 2017 reaching one million tons. Less than 10% of these are domestically consumed while more than 90% are exported to several countries whose population frequently consume longan as a nutritious food supplement. This study can help solving overproduction problem of longan fruit by processing C grade fresh longan, which is accounted for 5% of the overall production volume, in the form of juice extract with the relatively high sugars content. Microbial fermentation of this juice and subsequent whole cells biotransformation process result in ethanol and PAC, respectively. Ethanol can be used as an alternative biofuel or important industrial solvent while PAC is a precursor for production of commercial nasal decongestant (ephedrine) or anti‐asthmatic compound (pseudoephedrine).
Rojarej Nunta; Charin Techapun; Ampin Kuntiya; Prasert Hanmuangjai; Churairat Moukamnerd; Julaluk Khemacheewakul; Sumeth Sommanee; Alissara Reungsang; Mallika (Boonmee) Kongkeitkajorn; Noppol Leksawasdi. Ethanol and phenylacetylcarbinol production processes of Candida tropicalis TISTR 5306 and Saccharomyces cerevisiae TISTR 5606 in fresh juices from longan fruit of various sizes. Journal of Food Processing and Preservation 2018, 42, e13815 .
AMA StyleRojarej Nunta, Charin Techapun, Ampin Kuntiya, Prasert Hanmuangjai, Churairat Moukamnerd, Julaluk Khemacheewakul, Sumeth Sommanee, Alissara Reungsang, Mallika (Boonmee) Kongkeitkajorn, Noppol Leksawasdi. Ethanol and phenylacetylcarbinol production processes of Candida tropicalis TISTR 5306 and Saccharomyces cerevisiae TISTR 5606 in fresh juices from longan fruit of various sizes. Journal of Food Processing and Preservation. 2018; 42 (11):e13815.
Chicago/Turabian StyleRojarej Nunta; Charin Techapun; Ampin Kuntiya; Prasert Hanmuangjai; Churairat Moukamnerd; Julaluk Khemacheewakul; Sumeth Sommanee; Alissara Reungsang; Mallika (Boonmee) Kongkeitkajorn; Noppol Leksawasdi. 2018. "Ethanol and phenylacetylcarbinol production processes of Candida tropicalis TISTR 5306 and Saccharomyces cerevisiae TISTR 5606 in fresh juices from longan fruit of various sizes." Journal of Food Processing and Preservation 42, no. 11: e13815.
A bacterial halotolerant enzyme was characterized to understand the molecular mechanism of salt adaptation and to explore its protein engineering potential. Halotolerant serine protease (Apr_No16) from a newly isolated Bacillus subtilis strain no. 16 was characterized. Multiple alignments with previously reported non-halotolerant proteases, including subtilisin Carlsberg, indicated that Apr_No16 has eight acidic or polar amino acid residues that are replaced by nonpolar amino acids in non-halotolerant proteases. Those residues were hypothesized to be one of the primary contributors to salt adaptation. An eightfold mutant substituted with Ala residues exhibited 1.2- and 1.8-fold greater halotolerance at 12.5% (w/v) NaCl than Apr_No16 and Carlsberg, respectively. Amino acid substitution notably shifted the theoretical pI of the eightfold mutant, from 6.33 to 9.23, compared with Apr_No16. The resulting protein better tolerated high salt conditions. Changing the pI of a bacterial serine protease may be an effective strategy to improve the enzyme’s halotolerance.
Shinji Takenaka; Jyun Yoshinami; Ampin Kuntiya; Charin Techapun; Noppol Leksawasdi; Phisit Seesuriyachan; Thanongsak Chaiyaso; Masanori Watanabe; Kosei Tanaka; Ken-Ichi Yoshida. Characterization and mutation analysis of a halotolerant serine protease from a new isolate of Bacillus subtilis. Biotechnology Letters 2017, 40, 189 -196.
AMA StyleShinji Takenaka, Jyun Yoshinami, Ampin Kuntiya, Charin Techapun, Noppol Leksawasdi, Phisit Seesuriyachan, Thanongsak Chaiyaso, Masanori Watanabe, Kosei Tanaka, Ken-Ichi Yoshida. Characterization and mutation analysis of a halotolerant serine protease from a new isolate of Bacillus subtilis. Biotechnology Letters. 2017; 40 (1):189-196.
Chicago/Turabian StyleShinji Takenaka; Jyun Yoshinami; Ampin Kuntiya; Charin Techapun; Noppol Leksawasdi; Phisit Seesuriyachan; Thanongsak Chaiyaso; Masanori Watanabe; Kosei Tanaka; Ken-Ichi Yoshida. 2017. "Characterization and mutation analysis of a halotolerant serine protease from a new isolate of Bacillus subtilis." Biotechnology Letters 40, no. 1: 189-196.
A lactic acid producing bacterium, Lactobacillus rhamnosus M-23, newly isolated from a rice washing drainage storage tank was found to produce l-(+)-lactic acid from a non-sterilized mixture of rice washing drainage and rice bran without any additions of nutrients under the simultaneous saccharification and fermentation (SSF) process. This strain has the ability to utilize the non-sterilized rice washing drainage and rice bran as a source of carbohydrate, saccharifying enzymes and nutrients for lactic acid production. Observation of extracellular protease activity in SSF culture broth showed that a higher protease activity was present in strain M-23 than in other isolated lactic acid producing bacteria (LABs). To investigate the structural changes of solid particles of rice washing drainage throughout LAB cultivation, scanning electron microscopic (SEM) observation and Fourier transform infrared-spectroscopy (FT-IR) analysis were performed. The results of the SEM observation showed that the surface material could be removed from solid particles of rice washing drainage treated by culture broth (supernatant) of strain M-23, thus exposing the crystal structure of the starch particle surface. The results of the FT-IR analysis revealed that the specific transmittance decrease of the CC and CO stretching and OH group of the solid particles of the rice washing drainage were highly correlated with the produced lactic acid concentration and extracellular protease activity, respectively. These results demonstrate the high lactic acid producing ability of strain M-23 from a non-sterilized mixture of rice washing drainage and rice bran under the SSF condition due to the removal of proteinaceous material and exposure of the starch particle surface by extracellular protease.
Masanori Watanabe; Charin Techapun; Ampin Kuntiya; Noppol Leksawasdi; Phisit Seesuriyachan; Thanongsak Chaiyaso; Shinji Takenaka; Isamu Maeda; Masahiro Koyama; Kozo Nakamura. Extracellular protease derived from lactic acid bacteria stimulates the fermentative lactic acid production from the by-products of rice as a biomass refinery function. Journal of Bioscience and Bioengineering 2017, 123, 245 -251.
AMA StyleMasanori Watanabe, Charin Techapun, Ampin Kuntiya, Noppol Leksawasdi, Phisit Seesuriyachan, Thanongsak Chaiyaso, Shinji Takenaka, Isamu Maeda, Masahiro Koyama, Kozo Nakamura. Extracellular protease derived from lactic acid bacteria stimulates the fermentative lactic acid production from the by-products of rice as a biomass refinery function. Journal of Bioscience and Bioengineering. 2017; 123 (2):245-251.
Chicago/Turabian StyleMasanori Watanabe; Charin Techapun; Ampin Kuntiya; Noppol Leksawasdi; Phisit Seesuriyachan; Thanongsak Chaiyaso; Shinji Takenaka; Isamu Maeda; Masahiro Koyama; Kozo Nakamura. 2017. "Extracellular protease derived from lactic acid bacteria stimulates the fermentative lactic acid production from the by-products of rice as a biomass refinery function." Journal of Bioscience and Bioengineering 123, no. 2: 245-251.
Cassava starch was melt-blended with glycerol (70/30wt%/wt%) at 140°C to prepare thermoplastic starch (TPS). Chitosan (CTS) was premixed with starch and glycerol, in acidified water (lactic acid 2wt%), at 1, 5 and 10wt%/wt%. TPS/CTS was then melt-blended (160°C) with polyethylene-graft-maleic anhydride (PE-MAH). Phase determination and scanning electron microscopy indicated TPS/PE-MAH/CTS had a co-continuous morphology and CTS-induced phase inversion to give dispersed PE-MAH particles in a TPS matrix. Tensile strength at break and elongation, melt viscosity, fracture toughness and water contact angle of TPS/PE-MAH were improved by CTS incorporation. TPS/PE-MAH/CTS blends decreased the melting temperature of TPS and PE-MAH compared to the neat polymers. FTIR confirmed a reaction had occurred between amino groups (NH2) of CTS and the MAH groups of PE-MAH. This reaction and the enhanced miscibility between TPS and CTS improved the mechanical properties of the TPS/PE-MAH/CTS blend, particularly at 5wt%/wt% CTS.
Kittisak Jantanasakulwong; Noppol Leksawasdi; Phisit Seesuriyachan; Somchai Wongsuriyasak; Charin Techapun; Toshiaki Ougizawa. Reactive blending of thermoplastic starch and polyethylene-graft-maleic anhydride with chitosan as compatibilizer. Carbohydrate Polymers 2016, 153, 89 -95.
AMA StyleKittisak Jantanasakulwong, Noppol Leksawasdi, Phisit Seesuriyachan, Somchai Wongsuriyasak, Charin Techapun, Toshiaki Ougizawa. Reactive blending of thermoplastic starch and polyethylene-graft-maleic anhydride with chitosan as compatibilizer. Carbohydrate Polymers. 2016; 153 ():89-95.
Chicago/Turabian StyleKittisak Jantanasakulwong; Noppol Leksawasdi; Phisit Seesuriyachan; Somchai Wongsuriyasak; Charin Techapun; Toshiaki Ougizawa. 2016. "Reactive blending of thermoplastic starch and polyethylene-graft-maleic anhydride with chitosan as compatibilizer." Carbohydrate Polymers 153, no. : 89-95.
A crude xylanase preparation from Streptomyces thermovulgaris TISTR1948 was able to hydrolyze KOH-treated corncob and to produce bioactive xylooligosaccharides (XOs). A thermostable cellulase-free endo-xylanase from strain TISTR1948 was purified 15.0-fold from the crude preparation, with a recovery yield of 13.0%. On SDS-PAGE, the purified enzyme had an apparent molecular mass of 46.2 kDa. The N-terminal and internal amino acid sequences were determined and the cloned xylanase gene were sequenced. The 1,434-bp gene encodes a protein with a predicted molecular mass of 46,976 Da. The deduced amino acid sequence had a high degree of identity with the sequences of GH 10 xylanases from Streptomyces spp. The purified xylanase was highly stable within a pH range of 4.0–11.5 and was thermostable within a temperature range of 50–70 °C. The activity of the enzyme reached a maximum at 65 °C; the enzyme’s half-life was 90 min at 70 °C. Enzymatic activity was enhanced in the presence of metal ions, Ca2+, Co2+, and Mn2+ but almost completely inhibited by Hg2+, Pb2+, and SDS. The Km and Vmax values of the enzyme with beechwood xylan as the substrate were 37.55 μM and 303.03 U/mg, respectively. The crude, partially purified, and purified xylanases were assayed for XO production from KOH-treated corncob. The main component of the XO products was xylobiose, with very little xylose and arabinose. An in vitro evaluation of XOs from the purified xylanase showed that they enhanced the growth of probiotic Lactobacillus plantarum TISTR1465.
Pinpanit Boonchuay; Shinji Takenaka; Ampin Kuntiya; Charin Techapun; Noppol Leksawasdi; Phisit Seesuriyachan; Thanongsak Chaiyaso. Purification, characterization, and molecular cloning of the xylanase from Streptomyces thermovulgaris TISTR1948 and its application to xylooligosaccharide production. Journal of Molecular Catalysis B: Enzymatic 2016, 129, 61 -68.
AMA StylePinpanit Boonchuay, Shinji Takenaka, Ampin Kuntiya, Charin Techapun, Noppol Leksawasdi, Phisit Seesuriyachan, Thanongsak Chaiyaso. Purification, characterization, and molecular cloning of the xylanase from Streptomyces thermovulgaris TISTR1948 and its application to xylooligosaccharide production. Journal of Molecular Catalysis B: Enzymatic. 2016; 129 ():61-68.
Chicago/Turabian StylePinpanit Boonchuay; Shinji Takenaka; Ampin Kuntiya; Charin Techapun; Noppol Leksawasdi; Phisit Seesuriyachan; Thanongsak Chaiyaso. 2016. "Purification, characterization, and molecular cloning of the xylanase from Streptomyces thermovulgaris TISTR1948 and its application to xylooligosaccharide production." Journal of Molecular Catalysis B: Enzymatic 129, no. : 61-68.