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Dr. Alessandra Ferrandino
Dipartimento Colture Arboree, Università di Torino, via L. da Vinci 44, 10095 Grugliasco (TO), Italy

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0 Plant Biology
0 polyphenols
0 fruit quality
0 fruit ripening
0 Agricultural Science

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Journal article
Published: 01 August 2020 in Horticulture Research
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Flavescence dorée (FD) is a destructive phytoplasma disease of European grapevines. Spontaneous and cultivar-dependent recovery (REC) may occur in the field in FD-infected vines starting the year following the first symptoms. However, the biological underpinnings of this process are still largely unexplored. In this study, transcriptome sequencing (RNAseq), whole-genome bisulphite sequencing (WGBS) and metabolite analysis were combined to dissect molecular and metabolic changes associated to FD and REC in leaf veins collected in the field from healthy (H), FD and REC plants of the highly susceptible Vitis vinifera ‘Barbera’. Genes involved in flavonoid biosynthesis, carbohydrate metabolism and stress responses were overexpressed in FD conditions, whereas transcripts linked to hormone and stilbene metabolisms were upregulated in REC vines. Accumulation patterns of abscisic acid and stilbenoid compounds analysed in the same samples confirmed the RNAseq data. In recovery conditions, we also observed the persistence of some FD-induced expression changes concerning inhibition of photosynthetic processes and stress responses. Several differentially expressed genes tied to those pathways also underwent post-transcriptional regulation by microRNAs, as outlined by merging our transcriptomic data set with a previously conducted smallRNAseq analysis. Investigations by WGBS analysis also revealed different DNA methylation marks between REC and H leaves, occurring within the promoters of genes tied to photosynthesis and secondary metabolism. The results allowed us to advance the existence of a “molecular memory” of FDp infection, involving alterations in the DNA methylation status of REC plants potentially related to transcriptional reprogramming events, in turn triggering changes in hormonal and secondary metabolite profiles.

ACS Style

Chiara Pagliarani; Giorgio Gambino; Alessandra Ferrandino; Walter Chitarra; Urska Vrhovsek; Dario Cantu; Sabrina Palmano; Cristina Marzachì; Andrea Schubert. Molecular memory of Flavescence dorée phytoplasma in recovering grapevines. Horticulture Research 2020, 7, 1 -15.

AMA Style

Chiara Pagliarani, Giorgio Gambino, Alessandra Ferrandino, Walter Chitarra, Urska Vrhovsek, Dario Cantu, Sabrina Palmano, Cristina Marzachì, Andrea Schubert. Molecular memory of Flavescence dorée phytoplasma in recovering grapevines. Horticulture Research. 2020; 7 (1):1-15.

Chicago/Turabian Style

Chiara Pagliarani; Giorgio Gambino; Alessandra Ferrandino; Walter Chitarra; Urska Vrhovsek; Dario Cantu; Sabrina Palmano; Cristina Marzachì; Andrea Schubert. 2020. "Molecular memory of Flavescence dorée phytoplasma in recovering grapevines." Horticulture Research 7, no. 1: 1-15.

Journal article
Published: 25 June 2020 in Molecules
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The interest in the biological properties of grapevine polyphenols (PPs) in neuroprotection is continuously growing in the hope of finding translational applications. However, there are several concerns about the specificity of action of these molecules that appear to act non-specifically on the permeability of cellular membranes. Naturally occurring neuronal death (NOND) during cerebellar maturation is a well characterized postnatal event that is very useful to investigate the death and rescue of neurons. We here aimed to establish a baseline comparative study of the potential to counteract NOND of certain grapevine PPs of interest for the oenology. To do so, we tested ex vivo the neuroprotective activity of peonidin- and malvidin-3-O-glucosides, resveratrol, polydatin, quercetin-3-O-glucoside, (+)-taxifolin, and (+)-catechin. The addition of these molecules (50 μM) to organotypic cultures of mouse cerebellum explanted at postnatal day 7, when NOND reaches a physiological peak, resulted in statistically significant (two-tailed Mann–Whitney test—p < 0.001) reductions of the density of dead cells (propidium iodide+ cells/mm2) except for malvidin-3-O-glucoside. The stilbenes were less effective in reducing cell death (to 51–60%) in comparison to flavanols, (+)-taxifolin and quercetin 3-O-glucoside (to 69–72%). Thus, molecules with a -OH group in ortho position (taxifolin, quercetin 3-O-glucoside, (+)-catechin, and peonidin 3-O-glucoside) have a higher capability to limit death of cerebellar neurons. As NOND is apoptotic, we speculate that PPs act by inhibiting executioner caspase 3.

ACS Style

Laura Lossi; Adalberto Merighi; Vittorino Novello; Alessandra Ferrandino. Protective Effects of Some Grapevine Polyphenols against Naturally Occurring Neuronal Death. Molecules 2020, 25, 2925 .

AMA Style

Laura Lossi, Adalberto Merighi, Vittorino Novello, Alessandra Ferrandino. Protective Effects of Some Grapevine Polyphenols against Naturally Occurring Neuronal Death. Molecules. 2020; 25 (12):2925.

Chicago/Turabian Style

Laura Lossi; Adalberto Merighi; Vittorino Novello; Alessandra Ferrandino. 2020. "Protective Effects of Some Grapevine Polyphenols against Naturally Occurring Neuronal Death." Molecules 25, no. 12: 2925.

Journal article
Published: 22 June 2020 in Viruses
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Tomato plants can establish symbiotic interactions with arbuscular mycorrhizal fungi (AMF) able to promote plant nutrition and prime systemic plant defenses against pathogens attack; the mechanism involved is known as mycorrhiza-induced resistance (MIR). However, studies on the effect of AMF on viral infection, still limited and not conclusive, indicate that AMF colonization may have a detrimental effect on plant defenses against viruses, so that the term “mycorrhiza-induced susceptibility” (MIS) has been proposed for these cases. To expand the case studies to a not yet tested viral family, that is, Bromoviridae, we investigated the effect of the colonization by the AMF Funneliformis mosseae on cucumber mosaic virus (CMV) infection in tomato by phenotypic, physiological, biochemical, and transcriptional analyses. Our results showed that the establishment of a functional AM symbiosis is able to limit symptoms development. Physiological and transcriptomic data highlighted that AMF mitigates the drastic downregulation of photosynthesis-related genes and the reduction of photosynthetic CO2 assimilation rate caused by CMV infection. In parallel, an increase of salicylic acid level and a modulation of reactive oxygen species (ROS)-related genes, toward a limitation of ROS accumulation, was specifically observed in CMV-infected mycorrhizal plants. Overall, our data indicate that the AM symbiosis influences the development of CMV infection in tomato plants and exerts a priming effect able to enhance tolerance to viral infection.

ACS Style

Laura Miozzi; Anna Maria Vaira; Federico Brilli; Valerio Casarin; Mara Berti; Alessandra Ferrandino; Luca Nerva; Gian Paolo Accotto; Luisa Lanfranco. Arbuscular Mycorrhizal Symbiosis Primes Tolerance to Cucumber Mosaic Virus in Tomato. Viruses 2020, 12, 1 .

AMA Style

Laura Miozzi, Anna Maria Vaira, Federico Brilli, Valerio Casarin, Mara Berti, Alessandra Ferrandino, Luca Nerva, Gian Paolo Accotto, Luisa Lanfranco. Arbuscular Mycorrhizal Symbiosis Primes Tolerance to Cucumber Mosaic Virus in Tomato. Viruses. 2020; 12 (6):1.

Chicago/Turabian Style

Laura Miozzi; Anna Maria Vaira; Federico Brilli; Valerio Casarin; Mara Berti; Alessandra Ferrandino; Luca Nerva; Gian Paolo Accotto; Luisa Lanfranco. 2020. "Arbuscular Mycorrhizal Symbiosis Primes Tolerance to Cucumber Mosaic Virus in Tomato." Viruses 12, no. 6: 1.

Journal article
Published: 31 May 2020 in Applied Sciences
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This study investigates the impact of bunch microclimate on the evolution of some relevant carotenoids in Nebbiolo grapes. Four bunch-zone microclimates, defined by different vineyard aspect and vine vigor, were characterized by radiation and temperature indices. Berry samples were collected from green phase up to harvest, during two consecutive seasons and carotenoid determination was assessed by High-Performance Liquid Chromatography (HPLC). High carotenoid concentrations were highlighted in Nebbiolo. Lutein and neoxanthin contents (μg berry−1) varied similarly in both seasons achieving a concentration peak after veraison especially in the cooler plots while a variety effect on the lutein seasonal trend was presumed. Conversely, β-carotene content remained generally constant during ripening, with the exception of the south plots showing dissimilar evolution between the seasons. Furthermore, higher temperature in the less vigorous and south facing vineyards led to lower amounts of carotenoids, both during ripening and at harvest. Bunch zone temperature and light condition may affect both synthesis and degradation of grape carotenoids determining their amount and profile at harvest. These findings add further knowledge about the influence of climate changes on grape aroma precursors, and are useful to adapt cultural strategies and preserve grape quality consequently.

ACS Style

Andriani Asproudi; Maurizio Petrozziello; Silvia Cavalletto; Alessandra Ferrandino; Elena Mania; Silvia Guidoni. Bunch Microclimate Affects Carotenoids Evolution in cv. Nebbiolo (V. vinifera L.). Applied Sciences 2020, 10, 3846 .

AMA Style

Andriani Asproudi, Maurizio Petrozziello, Silvia Cavalletto, Alessandra Ferrandino, Elena Mania, Silvia Guidoni. Bunch Microclimate Affects Carotenoids Evolution in cv. Nebbiolo (V. vinifera L.). Applied Sciences. 2020; 10 (11):3846.

Chicago/Turabian Style

Andriani Asproudi; Maurizio Petrozziello; Silvia Cavalletto; Alessandra Ferrandino; Elena Mania; Silvia Guidoni. 2020. "Bunch Microclimate Affects Carotenoids Evolution in cv. Nebbiolo (V. vinifera L.)." Applied Sciences 10, no. 11: 3846.

Journal article
Published: 01 October 2019 in Scientia Horticulturae
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ACS Style

Olga Kedrina-Okutan; Vittorino Novello; Thomas Hoffmann; Johannes Hadersdorfer; Anna Schneider; Wilfried Schwab; Alessandra Ferrandino. Polyphenolic diversity in Vitis sp. leaves. Scientia Horticulturae 2019, 256, 1 .

AMA Style

Olga Kedrina-Okutan, Vittorino Novello, Thomas Hoffmann, Johannes Hadersdorfer, Anna Schneider, Wilfried Schwab, Alessandra Ferrandino. Polyphenolic diversity in Vitis sp. leaves. Scientia Horticulturae. 2019; 256 ():1.

Chicago/Turabian Style

Olga Kedrina-Okutan; Vittorino Novello; Thomas Hoffmann; Johannes Hadersdorfer; Anna Schneider; Wilfried Schwab; Alessandra Ferrandino. 2019. "Polyphenolic diversity in Vitis sp. leaves." Scientia Horticulturae 256, no. : 1.

Conference paper
Published: 01 April 2019 in BIO Web of Conferences
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The first serious outbreak of Flavescence dorée (FD) in Piedmont (North-West Italy) dates back to 1998 in the Tortona area (Alessandria province). FD is a serious quarantine-worthy disease transmitted by the leafhopper Scaphoideus titanus Ball. Different Vitis vinifera L. biotypes react differently to the phytoplasma, in particular as to the accumulation of polyphenols in leaves. In this experimentation, we observed and described concentration and accumulation of the main classes of polyphenols in entire leaves and in leaf blades and veins of two varieties, Nebbiolo and Barbera, displaying different levels of susceptibility to FD. Their well-known different reactions could be related, at least partially, to leaf polyphenols, both as to concentrations and profiles. Nebbiolo displayed some specific traits: i) the higher percentage of incidence over totals of individual molecules known to be powerful antioxidants (caftaric acid over coutaric acid; quercetin glycosides over other flavonols); ii) the higher concentration of flavanols in veins with respect to Barbera and their wider profile (astilbin and a taxifolin-glycoside, this last accumulating exclusively in Nebbiolo).

ACS Style

Alessandra Ferrandino; Chiara Pagliarani; Olga Kedrina-Okutan; Sara Icardi; Marco Bove; Claudio Lovisolo; Vittorino Novello; Andrea Schubert. Non-anthocyanin polyphenols in healthy and Flavescence dorée infected Barbera and Nebbiolo leaves. BIO Web of Conferences 2019, 13, 03003 .

AMA Style

Alessandra Ferrandino, Chiara Pagliarani, Olga Kedrina-Okutan, Sara Icardi, Marco Bove, Claudio Lovisolo, Vittorino Novello, Andrea Schubert. Non-anthocyanin polyphenols in healthy and Flavescence dorée infected Barbera and Nebbiolo leaves. BIO Web of Conferences. 2019; 13 ():03003.

Chicago/Turabian Style

Alessandra Ferrandino; Chiara Pagliarani; Olga Kedrina-Okutan; Sara Icardi; Marco Bove; Claudio Lovisolo; Vittorino Novello; Andrea Schubert. 2019. "Non-anthocyanin polyphenols in healthy and Flavescence dorée infected Barbera and Nebbiolo leaves." BIO Web of Conferences 13, no. : 03003.

Journal article
Published: 01 December 2018 in Food Chemistry
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In view of climate change, the scheduling of an early harvest may be an agronomic option to limit wine alcohol, provided that a satisfactory content of secondary metabolites can be ensured in grapes. To better understand the link between grape ripening, seasonal trend and wine aroma, the aromatic expression of Barbera and Pinot Noir wines produced with early harvested grapes was assessed. Attention was focused on C13 norisoprenoids during both alcoholic fermentation and after three months of storage. At the end of fermentation, the highest β-damascenone content was detected in wines obtained from less ripe grapes, the content subsequently increased significantly after three months of storage; however, the levels of β-ionone decreased significantly during the same period. The reduction of wine alcohol as a result of harvesting earlier, especially for Barbera, was associated with optimal aromatic levels as well as good technological parameters.

ACS Style

Andriani Asproudi; Alessandra Ferrandino; Federica Bonello; Enrico Vaudano; Matteo Pollon; Maurizio Petrozziello. Key norisoprenoid compounds in wines from early-harvested grapes in view of climate change. Food Chemistry 2018, 268, 143 -152.

AMA Style

Andriani Asproudi, Alessandra Ferrandino, Federica Bonello, Enrico Vaudano, Matteo Pollon, Maurizio Petrozziello. Key norisoprenoid compounds in wines from early-harvested grapes in view of climate change. Food Chemistry. 2018; 268 ():143-152.

Chicago/Turabian Style

Andriani Asproudi; Alessandra Ferrandino; Federica Bonello; Enrico Vaudano; Matteo Pollon; Maurizio Petrozziello. 2018. "Key norisoprenoid compounds in wines from early-harvested grapes in view of climate change." Food Chemistry 268, no. : 143-152.

Research article
Published: 03 September 2018 in Journal of Agricultural and Food Chemistry
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Despite the economic importance and the diffusion of grapevine cultivation worldwide, little is known about leaf chemical composition. We characterized the phenolic composition of Nebbiolo, Barbera, Pinot noir, Cabernet Sauvignon, Grenache and Shiraz (Vitis vinifera L.) healthy leaves (separating blades and veins) during the season. Quantitative and qualitative differences were found between leaf sectors and among genotypes. In healthy grapevine leaves, anthocyanins, dihydromyricetin-rhamnoside, hexosides of dihydroquercetin and dihydrokaempferol exclusively accumulated in veins. Astilbin was the only flavanonol detected in blades and the prevalent flavanonol in veins. Barbera distinguished for the lowest proanthocyanidin and the highest hydroxycinnamate content; Pinot noir for the absence of acylated-anthocyanins. Nebbiolo, Pinot noir and Cabernet Sauvignon displayed high concentration of epigallocatechin gallate. Nebbiolo leaves showed the highest concentrations of flavanonols and the widest profile differentiation. Knowledge derived from the present work is a contribution to find out leaf polyphenol potential as a part of grapevine defense mechanisms and to dissect genotype-related susceptibility to pathogens; moreover, it represents a starting point for future deepening about grapevine and vineyard by-products as a source of bioactive phenolic compounds.

ACS Style

Olga Kedrina-Okutan; Vittorino Novello; Thomas Hoffmann; Johannes Hadersdorfer; Andrea Occhipinti; Wilfried G. Schwab; Alessandra Ferrandino. Constitutive Polyphenols in Blades and Veins of Grapevine (Vitis vinifera L.) Healthy Leaves. Journal of Agricultural and Food Chemistry 2018, 66, 10977 -10990.

AMA Style

Olga Kedrina-Okutan, Vittorino Novello, Thomas Hoffmann, Johannes Hadersdorfer, Andrea Occhipinti, Wilfried G. Schwab, Alessandra Ferrandino. Constitutive Polyphenols in Blades and Veins of Grapevine (Vitis vinifera L.) Healthy Leaves. Journal of Agricultural and Food Chemistry. 2018; 66 (42):10977-10990.

Chicago/Turabian Style

Olga Kedrina-Okutan; Vittorino Novello; Thomas Hoffmann; Johannes Hadersdorfer; Andrea Occhipinti; Wilfried G. Schwab; Alessandra Ferrandino. 2018. "Constitutive Polyphenols in Blades and Veins of Grapevine (Vitis vinifera L.) Healthy Leaves." Journal of Agricultural and Food Chemistry 66, no. 42: 10977-10990.

Original article
Published: 28 July 2018 in Molecular Plant Pathology
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Plant virus infections are often difficult to characterize as they result from a complex molecular and physiological interplay between a pathogen and its host. In this study, the impact of the phloem‐limited grapevine virus B (GVB) on the Vitis vinifera L. wine‐red cultivar Albarossa was analysed under field conditions. Trials were carried out over two growing seasons by combining agronomic, molecular, biochemical and ecophysiological approaches. The data showed that GVB did not induce macroscopic symptoms on ‘Albarossa’, but affected the ecophysiological performances of vines in terms of assimilation rates, particularly at the end of the season, without compromising yield and vigour. In GVB‐infected plants, the accumulation of soluble carbohydrates in the leaves and transcriptional changes in sugar‐ and photosynthetic‐related genes seemed to trigger defence responses similar to those observed in plants infected by phytoplasmas, although to a lesser extent. In addition, GVB activated berry secondary metabolism. In particular, total anthocyanins and their acetylated forms accumulated at higher levels in GVB‐infected than in GVB‐free berries, consistent with the expression profiles of the related biosynthetic genes. These results contribute to improve our understanding of the multifaceted grapevine–virus interaction.

ACS Style

Walter Chitarra; Danila Cuozzo; Alessandra Ferrandino; Francesca Secchi; Sabrina Palmano; Irene Perrone; Paolo Boccacci; Chiara Pagliarani; Ivana Gribaudo; Franco Mannini; Giorgio Gambino. Dissecting interplays between Vitis vinifera L. and grapevine virus B (GVB) under field conditions. Molecular Plant Pathology 2018, 19, 2651 -2666.

AMA Style

Walter Chitarra, Danila Cuozzo, Alessandra Ferrandino, Francesca Secchi, Sabrina Palmano, Irene Perrone, Paolo Boccacci, Chiara Pagliarani, Ivana Gribaudo, Franco Mannini, Giorgio Gambino. Dissecting interplays between Vitis vinifera L. and grapevine virus B (GVB) under field conditions. Molecular Plant Pathology. 2018; 19 (12):2651-2666.

Chicago/Turabian Style

Walter Chitarra; Danila Cuozzo; Alessandra Ferrandino; Francesca Secchi; Sabrina Palmano; Irene Perrone; Paolo Boccacci; Chiara Pagliarani; Ivana Gribaudo; Franco Mannini; Giorgio Gambino. 2018. "Dissecting interplays between Vitis vinifera L. and grapevine virus B (GVB) under field conditions." Molecular Plant Pathology 19, no. 12: 2651-2666.

Journal article
Published: 01 March 2018 in Scientia Horticulturae
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The berry shrinking phenomenon in cv Shiraz (Vitis vinifera L.) is to date much debated. Currently, the critical points in Shiraz pre-harvest shrinkage are: a) the role of the xylem during post-véraison; b) the existence and timing of xylematic back flow and c) the functionality of the phloem. In order to try to resolve these issues, we traced the xylematic flows from the vine to the berry and vice versa by using the fuchsin acid as a xylematic tracer. At berry maturity, in order to verify also the phloematic functionality, we used the fluorescent tracer 6(5)-carboxyfluorescein diacetate (CFDA). The results showed clearly that the vine gradually loses the ability to deliver water to the berries via pedicel during ripening. The xylematic back-flow is active in the pre-véraison but not in the post-véraison berries. Furthermore, the CFDA experiments showed the absence of flow from the plant to the berry and vice versa at berry maturity. In cv Shiraz véraison seems to be the crucial point in the berry dehydration understanding: in pre-véraison there is a ‘plant/berry’ and ‘berry/plant’ water communication, whereas in post-véraison this seems to cease progressively.

ACS Style

Antonio Carlomagno; Vittorino Novello; Alessandra Ferrandino; Andrea Genre; Claudio Lovisolo; Jacobus J. Hunter. Pre-harvest berry shrinkage in cv ‘Shiraz’ (Vitis vinifera L.): Understanding sap flow by means of tracing. Scientia Horticulturae 2018, 233, 394 -406.

AMA Style

Antonio Carlomagno, Vittorino Novello, Alessandra Ferrandino, Andrea Genre, Claudio Lovisolo, Jacobus J. Hunter. Pre-harvest berry shrinkage in cv ‘Shiraz’ (Vitis vinifera L.): Understanding sap flow by means of tracing. Scientia Horticulturae. 2018; 233 ():394-406.

Chicago/Turabian Style

Antonio Carlomagno; Vittorino Novello; Alessandra Ferrandino; Andrea Genre; Claudio Lovisolo; Jacobus J. Hunter. 2018. "Pre-harvest berry shrinkage in cv ‘Shiraz’ (Vitis vinifera L.): Understanding sap flow by means of tracing." Scientia Horticulturae 233, no. : 394-406.

Journal article
Published: 19 April 2017 in Australian Journal of Grape and Wine Research
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Modern viticulture requires robust, fast, non-destructive methods to assess berry composition. We tested a chlorophyll fluorescence screening method to estimate berry phenolic substances. We focused on anthocyanin and flavonol in red and white cultivars. The ANTH_RG index was dependent on the cultivar anthocyanin profile. In Nebbiolo, in which dihydroxylated anthocyanins prevail, ANTH_RG was 2.4 times higher than in Barbera, in which trihydroxylated anthocyanins prevail. Considering the profiles of the two cultivars at similar anthocyanin concentration and their relative in vitro absorbance, a bathochromic shift of 10 nm emerged, which can explain the different screening effect exerted by anthocyanin on chlorophyll fluorescence. As to flavanols, we propose the calibration of a new spectroscopic index, the FLAV_UV, in coloured and white berries, finding good correlation with flavonol concentration determined analytically (R2 higher than 0.7). Spectroscopic indices can estimate the concentration of anthocyanin and flavonol in grape berries. A calibration curve for Nebbiolo, which has a distinctive anthocyanin profile, and the calibration of a new index, the FLAV_UV, able to estimate flavonol concentration in both red and white cultivars, are described for the first time. These indices can effectively be applied for non-destructive assessment of grape flavonoid.

ACS Style

A. Ferrandino; Chiara Pagliarani; A. Carlomagno; V. Novello; A. Schubert; G. Agati. Improved fluorescence-based evaluation of flavonoid in red and white winegrape cultivars. Australian Journal of Grape and Wine Research 2017, 23, 207 -214.

AMA Style

A. Ferrandino, Chiara Pagliarani, A. Carlomagno, V. Novello, A. Schubert, G. Agati. Improved fluorescence-based evaluation of flavonoid in red and white winegrape cultivars. Australian Journal of Grape and Wine Research. 2017; 23 (2):207-214.

Chicago/Turabian Style

A. Ferrandino; Chiara Pagliarani; A. Carlomagno; V. Novello; A. Schubert; G. Agati. 2017. "Improved fluorescence-based evaluation of flavonoid in red and white winegrape cultivars." Australian Journal of Grape and Wine Research 23, no. 2: 207-214.

Journal article
Published: 01 December 2016 in Food Chemistry
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Head space stir bar sorptive extraction (HS-SBSE) was used to evaluate the efficacy of two stir bar coatings, poly(ethylene glycol) (PEG)-modified silicone (EG-Silicone) and polyacrylate (PA), in comparison with polydimethyl siloxane (PDMS) coating, to assess volatiles in model wine solutions. In addition, two temperatures (0°C and 25°C) of the injection system (CIS) were compared. This was performed to propose an analytical procedure cheaper and faster respect to traditional extractive methods and environmental friendly, avoiding the use of large amounts of solvents and high-energy consumption for cooling. Fifteen grape volatiles with different polarities, known to have an important impact on varietal wine aroma, were tested. The affinity of each tested molecule to the new coatings was assessed. The PDMS coating showed higher relative areas for terpenes (β-citronellol, geraniol, linalool and α-terpineol) and norisoprenoids (β-ionone), especially when the CIS temperature was set at 25°C. C6 compounds such as 1-hexanol, (E)-2-hexenal, (E)-2-hexen-1-ol, (Z)-3-hexen-1-ol, and benzaldehyde and eugenol, obtained greater area response using EG-Silicone and PA coatings, regardless the temperatures. The memory effect of each coating type was studied; EG Silicone and PA coatings showed a higher memory effect for a certain compounds, probably due to the lower desorption temperature that EG Silicone and PA coatings can be submitted to, respect to PDMS (220°C the former two, 295°C the latter).

ACS Style

Kortes Serrano de la Hoz; Maria Rosario Salinas; Alessandra Ferrandino. Different coatings for the HS-SBSE grape volatile analysis in model solution: Preliminary results. Food Chemistry 2016, 212, 814 -820.

AMA Style

Kortes Serrano de la Hoz, Maria Rosario Salinas, Alessandra Ferrandino. Different coatings for the HS-SBSE grape volatile analysis in model solution: Preliminary results. Food Chemistry. 2016; 212 ():814-820.

Chicago/Turabian Style

Kortes Serrano de la Hoz; Maria Rosario Salinas; Alessandra Ferrandino. 2016. "Different coatings for the HS-SBSE grape volatile analysis in model solution: Preliminary results." Food Chemistry 212, no. : 814-820.

Journal article
Published: 13 June 2016 in Notulae Botanicae Horti Agrobotanici Cluj-Napoca
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The chemical composition of Romanian table grape varieties was determined to evaluate their phenolic and aroma profiles because they are factors useful for the variety characterization and consumer acceptance. Two white table grapes (‘Aromat de Iaşi’ and ‘Timpuriu de Cluj’) and two red table grapes (‘Napoca’ and ‘Someşan’) were studied. The phenolic composition of berry skins and seeds was determined using spectrophotometric methods. The profile of anthocyanins and hydroxycinnamoyl tartaric acids (HCTs), and the content of trans-resveratrol were determined in the skins using high-performance liquid chromatographic (HPLC) methods. Free volatile compounds were quantified by gas chromatography/mass spectrometry (GC/MS). A different phenolic and aromatic composition was found in the varieties studied. Among white grapes, ‘Aromat de Iaşi’ stands out for the great number of aroma compounds, and ‘Timpuriu de Cluj’ showed high contents of polyphenols, particularly oligomeric flavanols in skins and seeds (1,171 and 1,189 mg kg-1, respectively) and total skin HCTs (181.6 mg kg-1). The red variety ‘Napoca’ had high contents of total anthocyanins (380 mg kg-1) and total skin HCTs (183.2 mg kg-1), and it is characterized by high percentages of acylated anthocyanin derivatives (28.5%) and trans-caffeoyltartaric acid forms (58.8%). ‘Someşan’ grapes showed high contents of free volatile compounds, particularly of 1-hexanol, (Z)-3-hexen-1-ol and (E)-2-hexenoic acid (167.0, 59.4 and 167.0 µg kg-1). These results may contribute to the knowledge of Romanian table grapes perspective and to a better exploitation of these varieties.

ACS Style

Mihai Lucian Lung; Doru Pamfil; Nastasia Pop; Corina Catana; Simona Laura Lazar; Simone Giacosa; Fabrizio Torchio; Alessandra Ferrandino; Susana Rio Segade; Luca Rolle. Investigation on Phenolic and Aroma Compounds of Table Grapes from Romania. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2016, 44, 1 .

AMA Style

Mihai Lucian Lung, Doru Pamfil, Nastasia Pop, Corina Catana, Simona Laura Lazar, Simone Giacosa, Fabrizio Torchio, Alessandra Ferrandino, Susana Rio Segade, Luca Rolle. Investigation on Phenolic and Aroma Compounds of Table Grapes from Romania. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2016; 44 (1):1.

Chicago/Turabian Style

Mihai Lucian Lung; Doru Pamfil; Nastasia Pop; Corina Catana; Simona Laura Lazar; Simone Giacosa; Fabrizio Torchio; Alessandra Ferrandino; Susana Rio Segade; Luca Rolle. 2016. "Investigation on Phenolic and Aroma Compounds of Table Grapes from Romania." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 44, no. 1: 1.

Journal article
Published: 01 April 2016 in Plant Physiology and Biochemistry
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Drought stress affects anthocyanin accumulation and modification in vegetative and reproductive plant tissues. Anthocyanins are the most abundant flavonoids in grape (Vitis vinifera L.) coloured berry genotypes and are essential markers of grape winemaking quality. They are mostly mono- and di-methylated, such modifications increase their stability and improve berry quality for winemaking. Anthocyanin methylation in grape berries is induced by drought stress. A few caffeoyl-CoA O-methyltransferases (CCoAOMTs) active on anthocyanins have been described in grape. However, no drought-activated O-methyltransferases have been described in grape berries yet. In this study, we characterized VvCCoAOMT, a grapevine gene known to induce methylation of CoA esters in cultured grape cells. Transcript accumulation of VvCCoAOMT was detected in berry skins, and increased during berry ripening on the plant, and in cultured berries treated with ABA, concomitantly with accumulation of methylated anthocyanins, suggesting that anthocyanins may be substrates of this enzyme. Contrary as previously observed in cell cultures, biotic stress (Botrytis cinerea inoculation) did not affect VvCCoAOMT gene expression in leaves or berries, while drought stress increased VvCCoAOMT transcript in berries. The recombinant VvCCoAOMT protein showed in vitro methylating activity on cyanidin 3-O-glucoside. We conclude that VvCCoAOMT is a multifunctional O-methyltransferase that may contribute to anthocyanin methylation activity in grape berries, in particular under drought stress conditions.

ACS Style

Debora Giordano; Sofia Provenzano; Alessandra Ferrandino; Marco Vitali; Chiara Pagliarani; Federica Roman; Francesca Cardinale; Simone D. Castellarin; Andrea Schubert. Characterization of a multifunctional caffeoyl-CoA O -methyltransferase activated in grape berries upon drought stress. Plant Physiology and Biochemistry 2016, 101, 23 -32.

AMA Style

Debora Giordano, Sofia Provenzano, Alessandra Ferrandino, Marco Vitali, Chiara Pagliarani, Federica Roman, Francesca Cardinale, Simone D. Castellarin, Andrea Schubert. Characterization of a multifunctional caffeoyl-CoA O -methyltransferase activated in grape berries upon drought stress. Plant Physiology and Biochemistry. 2016; 101 ():23-32.

Chicago/Turabian Style

Debora Giordano; Sofia Provenzano; Alessandra Ferrandino; Marco Vitali; Chiara Pagliarani; Federica Roman; Francesca Cardinale; Simone D. Castellarin; Andrea Schubert. 2016. "Characterization of a multifunctional caffeoyl-CoA O -methyltransferase activated in grape berries upon drought stress." Plant Physiology and Biochemistry 101, no. : 23-32.

Journal article
Published: 22 February 2016 in Theoretical and Experimental Plant Physiology
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Grapevine adaptations to water-stress are described, by focusing on soil/root interactions and root-to-shoot signaling to control both plant water relations and fruit ripening process. Root response to drought, tolerance of available rootstock germoplasm, mechanisms of embolism formation and repair in root, aquaporin control of plant water relations, and abscisic acid biosynthesis and delivery are highlighted, by reviewing recent insights coming from either (eco)physiological literature or viticultural assays addressing vineyard-soil relationships.

ACS Style

Claudio Lovisolo; Anouk Lavoie-Lamoureux; Sara Tramontini; Alessandra Ferrandino. Grapevine adaptations to water stress: new perspectives about soil/plant interactions. Theoretical and Experimental Plant Physiology 2016, 28, 53 -66.

AMA Style

Claudio Lovisolo, Anouk Lavoie-Lamoureux, Sara Tramontini, Alessandra Ferrandino. Grapevine adaptations to water stress: new perspectives about soil/plant interactions. Theoretical and Experimental Plant Physiology. 2016; 28 (1):53-66.

Chicago/Turabian Style

Claudio Lovisolo; Anouk Lavoie-Lamoureux; Sara Tramontini; Alessandra Ferrandino. 2016. "Grapevine adaptations to water stress: new perspectives about soil/plant interactions." Theoretical and Experimental Plant Physiology 28, no. 1: 53-66.

Journal article
Published: 14 April 2015 in International Journal of Food Properties
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In this work, the chemical compositions and texture characteristics of three native Romanian wine grape varieties (Fetească regală, Fetească alba, and Fetească neagră) were studied. We assessed the distinct characteristics directly linked to their phenolic compositions, volatile profiles, and mechanical properties and compared these characteristics with those of Pinot noir grapes. The effect of the growing zone was also evaluated. Various spectrophotometric indices directly related to the phenolic compositions of berry skins and seeds were determined. The detailed phenolic compositions (anthocyanins, hydroxycinnamoyl tartaric acids, and stilbenes) of the skins were determined using high-performance liquid chromatography methods. Free and bound volatile compounds in the berries were quantified by gas chromatography/mass spectrometry. The textural properties of the skins and seeds were measured by instrumental texture analysis. The results showed high diversity among the varieties and zones that affected the enological potential. Among the white varieties, Fetească albă grapes could be less susceptible to browning as a consequence of their lower trans-caffeoyltartaric acid concentration, whereas Fetească regală grapes from Cluj had the highest concentrations of total free and bound volatile compounds, particularly terpenes and norisoprenoids. Among the red varieties, Fetească neagră was identified as a promising variety to be exploited in the future for its particular phenolic characteristics, particularly those grapes grown in Mica. Nevertheless, Fetească neagră grapes grown in Cluj had the highest total glycosidically bound terpene concentrations. Finally, differences in the mechanical and/or acoustic properties of the skins and seeds could strongly influence the kinetics and completeness of phenolic compound extractions.

ACS Style

Delia Elena Urcan; Mihai-Lucian Lung; Simone Giacosa; Fabrizio Torchio; Alessandra Ferrandino; Simone Vincenzi; Susana Río Segade; Nastasia Pop; Luca Rolle. Phenolic Substances, Flavor Compounds, and Textural Properties of Three Native Romanian Wine Grape Varieties. International Journal of Food Properties 2015, 19, 76 -98.

AMA Style

Delia Elena Urcan, Mihai-Lucian Lung, Simone Giacosa, Fabrizio Torchio, Alessandra Ferrandino, Simone Vincenzi, Susana Río Segade, Nastasia Pop, Luca Rolle. Phenolic Substances, Flavor Compounds, and Textural Properties of Three Native Romanian Wine Grape Varieties. International Journal of Food Properties. 2015; 19 (1):76-98.

Chicago/Turabian Style

Delia Elena Urcan; Mihai-Lucian Lung; Simone Giacosa; Fabrizio Torchio; Alessandra Ferrandino; Simone Vincenzi; Susana Río Segade; Nastasia Pop; Luca Rolle. 2015. "Phenolic Substances, Flavor Compounds, and Textural Properties of Three Native Romanian Wine Grape Varieties." International Journal of Food Properties 19, no. 1: 76-98.

Journal article
Published: 01 July 2014 in Environmental and Experimental Botany
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Alessandra Ferrandino; Claudio Lovisolo. Abiotic stress effects on grapevine (Vitis vinifera L.): Focus on abscisic acid-mediated consequences on secondary metabolism and berry quality. Environmental and Experimental Botany 2014, 103, 138 -147.

AMA Style

Alessandra Ferrandino, Claudio Lovisolo. Abiotic stress effects on grapevine (Vitis vinifera L.): Focus on abscisic acid-mediated consequences on secondary metabolism and berry quality. Environmental and Experimental Botany. 2014; 103 ():138-147.

Chicago/Turabian Style

Alessandra Ferrandino; Claudio Lovisolo. 2014. "Abiotic stress effects on grapevine (Vitis vinifera L.): Focus on abscisic acid-mediated consequences on secondary metabolism and berry quality." Environmental and Experimental Botany 103, no. : 138-147.

Journal article
Published: 15 May 2014 in Plant, Cell & Environment
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Flavescence dorée phytoplasma (FDp) infections seriously affect production and survival of grapevine. We analysed the changes in the flavonoid pathway occurring in two red cultivars, the highly susceptible Barbera and the less susceptible Nebbiolo, following FDp infection. A combination of metabolic and transcript analyses was used to quantify flavonoid compounds and expression of a set of genes involved in their biosynthesis. Quantification of anthocyanins, flavonols, proanthocyanidins and related biosynthetic enzymes was performed over the vegetative season, at four time points, on healthy, infected and recovered plants. A strong activation of anthocyanin accumulation was observed in infected Barbera leaves, while the response was less marked in Nebbiolo. Proanthocyanidins also accumulated mainly in infected Barbera leaves, even if basal proanthocyanidin concentration was higher in healthy and recovered Nebbiolo. Biochemical data were supported by transcript analysis: genes of the stem flavonoid pathway and of the anthocyanin and proanthocyanidin branches were expressed at a higher level in infected than in healthy plants, with a different magnitude between the two cultivars. Based on our results, we hypothesize that flavonoid accumulation is a physiological consequence of FD infection without affecting phytoplasma multiplication, although proanthocyanidin accumulation could help repel further infection by the insect vector.

ACS Style

Paolo Margaria; Alessandra Ferrandino; Piero Caciagli; Olga Kedrina; Andrea Schubert; Sabrina Palmano. Metabolic and transcript analysis of the flavonoid pathway in diseased and recovered Nebbiolo and Barbera grapevines (Vitis vinifera L.) following infection by Flavescence dorée phytoplasma. Plant, Cell & Environment 2014, 37, 2183 -2200.

AMA Style

Paolo Margaria, Alessandra Ferrandino, Piero Caciagli, Olga Kedrina, Andrea Schubert, Sabrina Palmano. Metabolic and transcript analysis of the flavonoid pathway in diseased and recovered Nebbiolo and Barbera grapevines (Vitis vinifera L.) following infection by Flavescence dorée phytoplasma. Plant, Cell & Environment. 2014; 37 (9):2183-2200.

Chicago/Turabian Style

Paolo Margaria; Alessandra Ferrandino; Piero Caciagli; Olga Kedrina; Andrea Schubert; Sabrina Palmano. 2014. "Metabolic and transcript analysis of the flavonoid pathway in diseased and recovered Nebbiolo and Barbera grapevines (Vitis vinifera L.) following infection by Flavescence dorée phytoplasma." Plant, Cell & Environment 37, no. 9: 2183-2200.

Research article
Published: 01 January 2014 in Functional Plant Biology
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Grapevine (Vitis vinifera L.) expresses different responses to water stress, depending not only on genotype, but also on the influence of vineyard growing conditions or seasonality. Our aim was to analyse the effects on drought response of two grapevine cultivars growing on two soils, one water draining (WD) containing sand 80% volume and the other water retaining (WR), with no sand. Under these two different water-holding capacities Syrah, displaying a near-anisohydric response to water stress, and Cabernet Sauvignon (on the contrary, near-isohydric) were submitted to water stress in a pot trial. Xylem embolism contributed to plant adaptation to soil water deprivation: in both cultivars during late phases of water stress, however, in Syrah, already at moderate early stress levels. By contrast, Syrah showed a less effective stomatal control of drought than Cabernet Sauvignon. The abscisic acid (ABA) influenced tightly the stomatal conductance of Cabernet Sauvignon on both pot soils. In the near-anisohydric variety Syrah an ABA-related stomatal closure was induced in WR soil to maintain high levels of water potential, showing that a soil-related hormonal root-to-shoot signal causing stomatal closure superimposes on the putatively variety-induced anisohydric response to water stress.

ACS Style

Sara Tramontini; Johanna Döring; Marco Vitali; Alessandra Ferrandino; Manfred Stoll; Claudio Lovisolo. Soil water-holding capacity mediates hydraulic and hormonal signals of near-isohydric and near-anisohydric Vitis cultivars in potted grapevines. Functional Plant Biology 2014, 41, 1119 -1128.

AMA Style

Sara Tramontini, Johanna Döring, Marco Vitali, Alessandra Ferrandino, Manfred Stoll, Claudio Lovisolo. Soil water-holding capacity mediates hydraulic and hormonal signals of near-isohydric and near-anisohydric Vitis cultivars in potted grapevines. Functional Plant Biology. 2014; 41 (11):1119-1128.

Chicago/Turabian Style

Sara Tramontini; Johanna Döring; Marco Vitali; Alessandra Ferrandino; Manfred Stoll; Claudio Lovisolo. 2014. "Soil water-holding capacity mediates hydraulic and hormonal signals of near-isohydric and near-anisohydric Vitis cultivars in potted grapevines." Functional Plant Biology 41, no. 11: 1119-1128.

Journal article
Published: 06 April 2013 in Journal of Experimental Botany
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Strigolactones (SLs) are newly identified hormones that regulate multiple aspects of plant development, infection by parasitic weeds, and mutualistic symbiosis in the roots. In this study, the role of SLs was studied for the first time in the model plant Lotus japonicus using transgenic lines silenced for CAROTENOID CLEAVAGE DIOXYGENASE 7 (LjCCD7), the orthologue of Arabidopsis More Axillary Growth 3. Transgenic LjCCD7-silenced plants displayed reduced height due to shorter internodes, and more branched shoots and roots than the controls, and an increase in total plant biomass, while their root:shoot ratio remained unchanged. Moreover, these lines had longer primary roots, delayed senescence, and reduced flower/pod numbers from the third round of flower and pod setting onwards. Only a mild reduction in determinate nodule numbers and hardly any impact on the colonization by arbuscular mycorrhizal fungi were observed. The results show that the impairment of CCD7 activity in L. japonicus leads to a phenotype linked to SL functions, but with specific features possibly due to the peculiar developmental pattern of this plant species. It is believed that the data also link determinate nodulation, plant reproduction, and senescence to CCD7 function for the first time.

ACS Style

Junwei Liu; Mara Novero; Tatsiana Charnikhova; Alessandra Ferrandino; Andrea Schubert; Carolien Ruyter-Spira; Paola Bonfante; Claudio Lovisolo; Harro J. Bouwmeester; Francesca Cardinale. CAROTENOID CLEAVAGE DIOXYGENASE 7 modulates plant growth, reproduction, senescence, and determinate nodulation in the model legume Lotus japonicus. Journal of Experimental Botany 2013, 64, 1967 -1981.

AMA Style

Junwei Liu, Mara Novero, Tatsiana Charnikhova, Alessandra Ferrandino, Andrea Schubert, Carolien Ruyter-Spira, Paola Bonfante, Claudio Lovisolo, Harro J. Bouwmeester, Francesca Cardinale. CAROTENOID CLEAVAGE DIOXYGENASE 7 modulates plant growth, reproduction, senescence, and determinate nodulation in the model legume Lotus japonicus. Journal of Experimental Botany. 2013; 64 (7):1967-1981.

Chicago/Turabian Style

Junwei Liu; Mara Novero; Tatsiana Charnikhova; Alessandra Ferrandino; Andrea Schubert; Carolien Ruyter-Spira; Paola Bonfante; Claudio Lovisolo; Harro J. Bouwmeester; Francesca Cardinale. 2013. "CAROTENOID CLEAVAGE DIOXYGENASE 7 modulates plant growth, reproduction, senescence, and determinate nodulation in the model legume Lotus japonicus." Journal of Experimental Botany 64, no. 7: 1967-1981.