Targeted profiling of polyphenols in trees may reveal valuable sources of natural compounds with major applications in pharmacology and disease control. The current study targeted the profiling of polyphenols using HPLC-DAD in Quercus robur, Q. macrocarpa and Q. acutissima bark extracts. Free radical scavenging of each extract was investigated using antioxidant assays. Antimicrobial activities against a wide spectrum of bacteria and fungi were explored, as well as anticancer activities against different cancer cell lines. The HPLC-DAD analyses revealed the availability of several polyphenols in high amounts, including ellagic acid (in Q. robur) and caffeic acid (in Q. macrocarpa) in all three species. The bioactivity assay revealed high antioxidant activity in Q. robur compared to that of the other species, as well as phenolic standards. The three oak bark extracts showed clear antibacterial activities against most bacteria tested, with the highest antibacterial activities in the extracts of Q. robur. In addition, the three extracts showed higher antibacterial activities against Pseudomonas aeruginosa, Micrococcus flavus, and Escherichia coli compared to that of other bacteria. There were strong antifungal activities against some fungi, such as Aspergillus flavus, Penicillium funiculosum, and Penicillium ochrochloron. There were also noticeable anticancer activities against MCF-7, HeLa, Jurkat, and HT-29 cell lines, with the highest anticancer activity in the extracts of Q. robur. This is the first study that reveals not only novel sources of important polyphenols (e.g., ellagic acid) in Q. robur, Q. macrocarpa and Q. acutissima bark but also their anticancer activities against diverse cancer cell lines.

The energy consumption pattern and greenhouse gas (GHG) emission of any rice production system is important to know the sustainability of varied cultivation and establishment technique. This study was conducted to determine the energy use pattern, GHG emission and efficiency of rice farms in puddled transplanted (PTR, rainfed) and direct-seeded rice (DSR, irrigated) production systems in Karnataka, India. The energy indices and GHG emission of different input and output in a rice production system were assessed by using energy and carbon equivalence. The efficiency of PTR and DSR farms were identified using data envelopment analysis (DEA) and energy optimization was ascertained. The key finding was excessive use of non-renewable energy inputs was observed for the PTR (92.4%) compare to DSR (60.3%) methods. The higher energy use efficiency (7.3), energy productivity (0.3 kg MJ⁻¹) and energy profitability (6.3) were mainly attributed to the large decrease in energy inputs under DSR. The DEA showed efficiency for 26 PTR farms in comparison for 87 DSR farms. The mean technical efficiency value highlighted the scope for saving energy by 6% and 2% in PTR and DSR, respectively and showed an economic reduction of $405.5/ha with PTR versus $163.3/ha with the DSR method if these inefficient farms perform efficiently. The GHG emissions revealed that the total emissions for PTR versus DSR production caused by on-farm emissions were 86% and 65%, respectively. The DSR method also had a higher carbon efficiency ratio and carbon sustainability index (10.1 and 9.1, respectively). Thus, adoption of DSR method is imperative for reduction of energy consumption and GHG emissions to achieve the carbon sustainability.

New sources of polyphenols with anticancer, antioxidant, and antimicrobial properties in arid environments are critical for the development of alternative medicines and natural remedies. This study explored the polyphenol profiles and biological activities of methanolic leaf extracts from natural Mentha × piperita and Mentha longifolia populations in northern Saudi Arabia. Chromatographic analyses identified several polyphenols in M. × piperita including phenolic acids: rosmarinic acid (1547.6 mg/100 g DW (dry weight)), cryptochlorogenic acid (91.7 mg/100 g DW), and chlorogenic acid (69.4 mg/100 g DW), as well as flavonoids: naringin (328.8 mg/100 g DW) and cynaroside (162.8 mg/100 g DW). The major polyphenols in M. longifolia were: rosmarinic acid (781.6 mg/100 g DW), cryptochlorogenic acid (191.1 mg/100 g DW), p-coumaric acid (113.0 mg/100 g DW), m-coumaric acid (112.2 mg/100 g DW), and chlorogenic acid (63.8 mg/100 g DW). M. × piperita and M. longifolia leaf extracts had high antioxidant activities due to the major polyphenols (cynaroside, rosmarinic and cryptochlorogenic acids). M. × piperita had higher activities against different cancer cells than M. longifolia. Naringin, cryptochlorogenic acid, and rosmarinic acid had the highest activities against cancer cells. The leaf extracts had antibacterial effects against most bacteria species (Pseudomonas aeruginosa was most sensitive), which was attributed to the polyphenols. Antifungal activities were similarly broad (Aspergillus flavus was most sensitive) and attributed to naringin, cryptochlorogenic acid, and caffeic acid. Populations of M. × piperita and M. longifolia in Northern Riyadh may be a valuable source of natural biologically active compounds.

Salt stress significantly impacts plant morphological structure and physiological processes, resulting in decreased plant growth. Salicylic acid (SA) is a key signal molecule that protects plants from the negative impacts of salinity. Under natural conditions, the pomegranate plant generally exhibits salt-tolerant characteristics. The objective of this study was to elucidate the salt-tolerance level of pomegranate (Punica granatum L. cv. Wonderful) and the effect of the regulating strategy of SA foliar spray on growth, morphological structure, and physiological processes. SA levels were 0, 0.25, 0.50, and 1 mM in the presence of salinity levels of 10, 35, and 70 mM NaCl, respectively. Vegetative growth indices, including stem cross-sectional area, leaf area, and total dry weight, were lowered by salinity treatments. However, SA applications greatly improved morphological characteristics and plant growth under salt stress. The effects of salinity were effectively reversed by SA treatment at 1 mM compared to control and other treatments. Interestingly, SA applications enhanced the chlorophyll, total phenolic, carbohydrate, and proline contents of leaves while decreasing electrolyte leakage (EL), Na, and Cl levels. Moreover, the foliar SA treatments enhanced the nutrient content in the leaves and increased the activities of peroxidase (POD) and catalase (CAT), with a decrease in malondialdehyde (MDA) content. This study suggests that the alleviation of the salinity stress by SA may be due to the activation of the antioxidant enzymatic mechanism and decrease in the lipid peroxidation of the pomegranate plant.

Natural biostimulants combine different elicitors that may influence economic properties of herbal crops, such as mint. Mint (Mentha longifolia L.) plants were subjected to three water levels based on container substrate capacity (CSC; 100% CSC, 70% CSC, and 50% CSC) and/or applications of four biostimulants (CRADLE™, Mobilizer™, Nanozim De’Lite™ [ND], and Nanozim NXT™ [NN]). ND and NN exhibited higher vegetative growth and root dry weight than the control (without biostimulants) and other treatments. NN produced the highest fresh and dry mint yields under all water levels. Irrigation water-use efficiency (IWUE) of NN was highest (2.78 kg m⁻³) with 70% CSC, whereas the control produced the lowest IWUE (1.85 kg m⁻³) with 100% CSC. Biostimulants boosted physiological and metabolic responses, including gas exchange, leaf water potential, relative water content, and proline accumulation of stressed plants. NN treatment with 70% CSC had the highest essential oil (EO) ratio (3.35%). Under 70% and 50% CSC with NN treatment, the proportion of 1,8-cineol increased and that of pulegone decreased in EOs. Increased antioxidant activities, reduced H₂O₂ levels, and increased catalase and superoxide dismutase activities were observed. Applications of ND and NN during water stress conditions increased economic and medicinal properties of mint EOs with applications in the agricultural and pharmaceutical industries.

The synergistic effect of dipping in 55 °C for 5 min of hot water (HW) and 1% chitosan coating during the storage of mango at 13 ± 0.5 °C and 85%–90% relative humidity for 28 days was investigated. The combined treatment significantly suppressed the fruit decay percentage compared with both the single treatment and the control. In addition, the specific activities of key plant defense-related enzymes, including peroxidase (POD) and catalase (CAT), markedly increased. The increase occurred in the pulp of the fruits treated with the combined treatment compared to those treated with HW or chitosan alone. While the control fruits showed the lowest values, the combination of pre-storage HW treatment and chitosan coating maintained higher values of flesh hue angle (h°), vitamin C content, membrane stability index (MSI) percentage, as well as lower weight loss compared with the untreated mango fruits. The combined treatment and chitosan treatment alone delayed fruit ripening by keeping fruit firmness, lessening the continuous increase of total soluble solids (TSS), and slowing the decrease in titratable acidity (TA). The results showed that the combined application of HW treatment and chitosan coating can be used as an effective strategy to suppress postharvest decay and improve the quality of mango fruits.