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Traditional herbal medicines have become a subject of global importance with both medical and economic implications. The regular consumption of herbal drugs has led to serious concerns regarding their quality, effectiveness, and safety. Thus, relevant scientific evidence has become an important criterion for the acceptance of traditional health claims. The genus Cotoneaster Medikus provides numerous species traditionally used in Asian medicine for the treatment of haemorrhoids, diabetes, and cardiovascular diseases. This review summarises the achievements of modern research on the Cotoneaster taxa, including ethnobotany, phytochemistry, pharmacology, and toxicology. To date, more than 90 compounds have been isolated or analytically identified in Cotoneaster leaves, fruits, flowers or twigs. These phytochemicals are categorised into flavonoids, procyanidins, phenolic acids, cotonefurans, cyanogenic glycosides, triterpenes, sterols, fatty acids, volatile compounds, and carbohydrates, and many of them are responsible for Cotoneaster pharmacological properties including antioxidant, anti-inflammatory, antimicrobial, antiparasitic, hepatoprotective, anti-diabetic or anti-dyslipidaemic activity. In order to ensure the safety of pharmaceutical applications, the potential toxicity of Cotoneaster extracts has also been investigated. In conclusion, this systematic review provides an important reference base for further study into the various medical applications of both the dry extracts and pure isolates of Cotoneaster species.
Agnieszka Kicel. An Overview of the Genus Cotoneaster (Rosaceae): Phytochemistry, Biological Activity, and Toxicology. Antioxidants 2020, 9, 1002 .
AMA StyleAgnieszka Kicel. An Overview of the Genus Cotoneaster (Rosaceae): Phytochemistry, Biological Activity, and Toxicology. Antioxidants. 2020; 9 (10):1002.
Chicago/Turabian StyleAgnieszka Kicel. 2020. "An Overview of the Genus Cotoneaster (Rosaceae): Phytochemistry, Biological Activity, and Toxicology." Antioxidants 9, no. 10: 1002.
Cotoneaster plants are sources of traditional medicines and dietary products, with health benefits resulting from their phenolic contents and antioxidant activity. In this work, active markers of the leaves of C. bullatus and C. zabelii were characterized and evaluated in an integrated phytochemical and biological activity study. Based on UHPLC-PDA-ESI-MS3 analysis, twelve analytes were preselected from the constituents of the hydromethanolic leaf extracts, and two of them—caffeoylmalic acid and quercetin 3--O-β-d-(2″--O-β-d-xylopyranosyl)galactopyranoside (QPH)—were isolated for full identification (NMR spectroscopy: 1H, 13C, COSY, HMBC, HMQC). All selected phenolics contributed to the antioxidant activity of the extracts, which was demonstrated in chemical in vitro tests (DPPH, FRAP, and TBARS) and in a biological model of human plasma exposed to oxidative/nitrative stress induced by peroxynitrite. This contribution was partly due to the synergy between individual polyphenols, evidenced by an isobolographic analysis of the interactions of (–)-epicatechin, chlorogenic acid, and QPH as representatives of three classes of Cotoneaster polyphenols. All twelve markers, including also neochlorogenic acid, cryptochlorogenic acid, procyanidin B2, procyanidin C1, rutin, hyperoside, isoquercitrin, and quercitrin, were thus applied as calibration standards, and a fast, accurate, reproducible, and fully validated RP-HPLC-PDA method for quality control and standardization of the target extracts was proposed.
Agnieszka Kicel; Aleksandra Owczarek; Paulina Kapusta; Joanna Kolodziejczyk-Czepas; Monika A. Olszewska. Contribution of Individual Polyphenols to Antioxidant Activity of Cotoneaster bullatus and Cotoneaster zabelii Leaves—Structural Relationships, Synergy Effects and Application for Quality Control. Antioxidants 2020, 9, 69 .
AMA StyleAgnieszka Kicel, Aleksandra Owczarek, Paulina Kapusta, Joanna Kolodziejczyk-Czepas, Monika A. Olszewska. Contribution of Individual Polyphenols to Antioxidant Activity of Cotoneaster bullatus and Cotoneaster zabelii Leaves—Structural Relationships, Synergy Effects and Application for Quality Control. Antioxidants. 2020; 9 (1):69.
Chicago/Turabian StyleAgnieszka Kicel; Aleksandra Owczarek; Paulina Kapusta; Joanna Kolodziejczyk-Czepas; Monika A. Olszewska. 2020. "Contribution of Individual Polyphenols to Antioxidant Activity of Cotoneaster bullatus and Cotoneaster zabelii Leaves—Structural Relationships, Synergy Effects and Application for Quality Control." Antioxidants 9, no. 1: 69.
Complementary UHPLC-PDA-ESI-MS3, HPLC-PDA-fingerprint, Folin-Ciocalteau, n-butanol/HCl and hide powder assays were employed to study the qualitative and quantitative differences in phenolic composition between different organs (leaves, flowers, bark and fruits) of Cotoneaster integerrimus. The bark and flowers were the richest source of polyphenols (107.0–113.6 mg GAE/g dw of plant material), with the dominating proanthocyanidins (96.3–97.7 mg/g dw), and low-molecular weight flavan-3-ols (21.5–22.0 mg/g). Among the leaf phenolics (93.6 mg/g), caffeoylquinic pseudodepsides (21.0 mg/g) and flavonol glycosides (21.7 mg/g) were prevailing, while the fruits contained relatively low amounts of polyphenols (49.5 mg/g) with high proportion of procyanidins (27.1 mg/g). In in vitro tests of antioxidant activity (DPPH, FRAP and TBARS), the effects of bark, flowers and leaves were significantly higher in comparison to these of the fruits. The bark sample had also the highest anti-inflammatory activity in the inhibition tests towards lipoxygenase and hyaluronidase. Despite the differences, the composition of the investigated organs seems consistent with the indications of traditional medicine and supports their therapeutic application in inflammation/infectious diseases and cardiovascular disorders.
Agnieszka Kicel; Aleksandra Owczarek; Paulina Gralak; Pawel Ciszewski; Monika A. Olszewska. Polyphenolic profile, antioxidant activity, and pro-inflammatory enzymes inhibition of leaves, flowers, bark and fruits of Cotoneaster integerrimus: A comparative study. Phytochemistry Letters 2019, 30, 349 -355.
AMA StyleAgnieszka Kicel, Aleksandra Owczarek, Paulina Gralak, Pawel Ciszewski, Monika A. Olszewska. Polyphenolic profile, antioxidant activity, and pro-inflammatory enzymes inhibition of leaves, flowers, bark and fruits of Cotoneaster integerrimus: A comparative study. Phytochemistry Letters. 2019; 30 ():349-355.
Chicago/Turabian StyleAgnieszka Kicel; Aleksandra Owczarek; Paulina Gralak; Pawel Ciszewski; Monika A. Olszewska. 2019. "Polyphenolic profile, antioxidant activity, and pro-inflammatory enzymes inhibition of leaves, flowers, bark and fruits of Cotoneaster integerrimus: A comparative study." Phytochemistry Letters 30, no. : 349-355.
Anna Magiera; Monika Sienkiewicz; Monika Olszewska; Agnieszka Kicel; Piotr Michel. Chemical profile and antibacterial activity of essential oils from leaves and fruits of Gaultheria procumbens L. cultivated in Poland. Acta Poloniae Pharmaceutica - Drug Research 2019, 76, 93 -102.
AMA StyleAnna Magiera, Monika Sienkiewicz, Monika Olszewska, Agnieszka Kicel, Piotr Michel. Chemical profile and antibacterial activity of essential oils from leaves and fruits of Gaultheria procumbens L. cultivated in Poland. Acta Poloniae Pharmaceutica - Drug Research. 2019; 76 (1):93-102.
Chicago/Turabian StyleAnna Magiera; Monika Sienkiewicz; Monika Olszewska; Agnieszka Kicel; Piotr Michel. 2019. "Chemical profile and antibacterial activity of essential oils from leaves and fruits of Gaultheria procumbens L. cultivated in Poland." Acta Poloniae Pharmaceutica - Drug Research 76, no. 1: 93-102.
Rhaponticum carthamoides (Willd.) Iljin (Asteraceae) is an endemic plant species whose roots and rhizomes have been used for many years in traditional Siberian medicine. In this study, the transformed roots of R. carthamoides obtained after Agrobacterium rhizogenes transformation were able to spontaneously regenerate green buds and shoots. Bud and shoot regeneration was observed in more than 90% of the cultures grown in Schenk and Hildebrandt (SH) or Gamborg (B5) liquid medium with full micro- and macronutrient concentrations, under light conditions. The regeneration of shoots from transformed roots may be improved by supplementation of Murashige and Skoog (MS) agar medium with indole-3-acetic acid (IAA) and benzyladenine (BA). The shoots were multiplied on MS medium supplemented with 0.1 mg/L IAA and 0.2 mg/L BA and then rooted successfully. The transformation of the plants derived from transformed roots was confirmed by PCR analysis and Southern hybridization. The three-month-old pRi-transformed plants (TR-plants) were able to biosynthesize caffeoylquinic acid derivatives such as chlorogenic acid, and 3,5- or 4,5-di-O-caffeoylquinic acids. Plants obtained directly from seeds (SD-plants) being in the same age and grown under the same conditions as the transformed plants were used as compared material. The transformed plants demonstrated higher secondary metabolite levels and greater biomass accumulation than SD-plants. It was found that extracts from TR-plants exhibited antioxidant properties in the model of human blood plasma and inhibited the growth of A2058 human melanoma cells depending on the tested concentration. The present study demonstrated that the pRi-transformed plants of R. carthamoides may be an alternative to conventional field crops and these plants can be used as the promising source of the valuable compounds, such as caffeoylquinic acid derivatives.
Ewa Skała; Laurent Picot; Michał Bijak; Joanna Saluk-Bijak; Janusz Szemraj; Agnieszka Kicel; Monika A. Olszewska; Przemysław Sitarek. An efficient plant regeneration from Rhaponticum carthamoides transformed roots, enhanced caffeoylquinic acid derivatives production in pRi-transformed plants and their biological activity. Industrial Crops and Products 2018, 129, 327 -338.
AMA StyleEwa Skała, Laurent Picot, Michał Bijak, Joanna Saluk-Bijak, Janusz Szemraj, Agnieszka Kicel, Monika A. Olszewska, Przemysław Sitarek. An efficient plant regeneration from Rhaponticum carthamoides transformed roots, enhanced caffeoylquinic acid derivatives production in pRi-transformed plants and their biological activity. Industrial Crops and Products. 2018; 129 ():327-338.
Chicago/Turabian StyleEwa Skała; Laurent Picot; Michał Bijak; Joanna Saluk-Bijak; Janusz Szemraj; Agnieszka Kicel; Monika A. Olszewska; Przemysław Sitarek. 2018. "An efficient plant regeneration from Rhaponticum carthamoides transformed roots, enhanced caffeoylquinic acid derivatives production in pRi-transformed plants and their biological activity." Industrial Crops and Products 129, no. : 327-338.
The work presents the results of an investigation into the molecular background of the activity of Cotoneaster fruits, providing a detailed description of their phytochemical composition and some of the mechanisms of their anti-inflammatory and antioxidant effects. GS-FID-MS and UHPLC-PDA-ESI-MS3 methods were applied to identify the potentially health-beneficial constituents of lipophilic and hydrophilic fractions, leading to the identification of fourteen unsaturated fatty acids (with dominant linoleic acid, 375.4–1690.2 mg/100 g dw), three phytosterols (with dominant β-sitosterol, 132.2–463.3 mg/100 g), two triterpenoid acids (10.9–54.5 mg/100 g), and twenty-six polyphenols (26.0–43.5 mg GAE/g dw). The most promising polyphenolic fractions exhibited dose-dependent anti-inflammatory activity in in vitro tests of lipoxygenase (IC50 in the range of 7.7–24.9 μg/U) and hyaluronidase (IC50 in the range of 16.4–29.3 μg/U) inhibition. They were also demonstrated to be a source of effective antioxidants, both in in vitro chemical tests (DPPH, FRAP, and TBARS) and in a biological model, in which at in vivo-relevant levels (1–5 μg/mL) they normalized/enhanced the nonenzymatic antioxidant capacity of human plasma and efficiently protected protein and lipid components of plasma against peroxynitrite-induced oxidative/nitrative damage. Moreover, the investigated extracts did not exhibit cytotoxicity towards human PMBCs. Among the nine Cotoneaster species tested, C. hjelmqvistii, C. zabelii, C. splendens, and C. bullatus possess the highest bioactive potential and might be recommended as dietary and functional food products.
Agnieszka Kicel; Joanna Kolodziejczyk-Czepas; Aleksandra Owczarek; Magdalena Rutkowska; Anna Wajs-Bonikowska; Sebastian Granica; Pawel Nowak; Monika A. Olszewska. Multifunctional Phytocompounds in Cotoneaster Fruits: Phytochemical Profiling, Cellular Safety, Anti-Inflammatory and Antioxidant Effects in Chemical and Human Plasma Models In Vitro. Oxidative Medicine and Cellular Longevity 2018, 2018, 1 -16.
AMA StyleAgnieszka Kicel, Joanna Kolodziejczyk-Czepas, Aleksandra Owczarek, Magdalena Rutkowska, Anna Wajs-Bonikowska, Sebastian Granica, Pawel Nowak, Monika A. Olszewska. Multifunctional Phytocompounds in Cotoneaster Fruits: Phytochemical Profiling, Cellular Safety, Anti-Inflammatory and Antioxidant Effects in Chemical and Human Plasma Models In Vitro. Oxidative Medicine and Cellular Longevity. 2018; 2018 ():1-16.
Chicago/Turabian StyleAgnieszka Kicel; Joanna Kolodziejczyk-Czepas; Aleksandra Owczarek; Magdalena Rutkowska; Anna Wajs-Bonikowska; Sebastian Granica; Pawel Nowak; Monika A. Olszewska. 2018. "Multifunctional Phytocompounds in Cotoneaster Fruits: Phytochemical Profiling, Cellular Safety, Anti-Inflammatory and Antioxidant Effects in Chemical and Human Plasma Models In Vitro." Oxidative Medicine and Cellular Longevity 2018, no. : 1-16.
The present study investigated the phenolic profile and biological activity of dry extracts from leaves of C. bullatus, C. zabelii and C. integerrimus—traditional medicinal and dietary plants—and evaluated their potential in adjunctive therapy of cardiovascular diseases. Complementary UHPLC-PDA-ESI-MS3, HPLC-PDA-fingerprint, Folin-Ciocalteu, and n-butanol/HCl assays of the extracts derived by fractionated extraction confirmed that they are rich in structurally diverse polyphenols (47 analytes, content up to 650.8 mg GAE/g dw) with proanthocyanidins (83.3–358.2 mg CYE/g) dominating in C. bullatus and C. zabelii, and flavonoids (53.4–147.8 mg/g) in C. integerrimus. In chemical in vitro tests of pro-inflammatory enzymes (lipoxygenase, hyaluronidase) inhibition and antioxidant activity (DPPH, FRAP), the extracts effects were dose-, phenolic- and extraction solvent-dependent. The most promising polyphenolic extracts were demonstrated to be effective antioxidants in a biological model of human blood plasma—at in vivo-relevant levels (1–5 µg/mL) they normalized/enhanced the non-enzymatic antioxidant capacity of plasma and effectively prevented peroxynitrite-induced oxidative/nitrative damage of plasma proteins and lipids. As demonstrated in cytotoxicity tests, the extracts were safe—they did not affect viability of human peripheral blood mononuclear cells. In conclusion, Cotoneaster leaves may be useful in development of natural-based products, supporting the treatment of oxidative stress/inflammation-related chronic diseases, including cardiovascular disorders.
Agnieszka Kicel; Joanna Kolodziejczyk-Czepas; Aleksandra Owczarek; Anna Marchelak; Malgorzata Sopinska; Pawel Ciszewski; Pawel Nowak; Monika A. Olszewska. Polyphenol-Rich Extracts from Cotoneaster Leaves Inhibit Pro-Inflammatory Enzymes and Protect Human Plasma Components against Oxidative Stress In Vitro. Molecules 2018, 23, 2472 .
AMA StyleAgnieszka Kicel, Joanna Kolodziejczyk-Czepas, Aleksandra Owczarek, Anna Marchelak, Malgorzata Sopinska, Pawel Ciszewski, Pawel Nowak, Monika A. Olszewska. Polyphenol-Rich Extracts from Cotoneaster Leaves Inhibit Pro-Inflammatory Enzymes and Protect Human Plasma Components against Oxidative Stress In Vitro. Molecules. 2018; 23 (10):2472.
Chicago/Turabian StyleAgnieszka Kicel; Joanna Kolodziejczyk-Czepas; Aleksandra Owczarek; Anna Marchelak; Malgorzata Sopinska; Pawel Ciszewski; Pawel Nowak; Monika A. Olszewska. 2018. "Polyphenol-Rich Extracts from Cotoneaster Leaves Inhibit Pro-Inflammatory Enzymes and Protect Human Plasma Components against Oxidative Stress In Vitro." Molecules 23, no. 10: 2472.
Aim of the study: Taking into account that overactivated leukocytes are an important factor in the development of many chronic diseases, we investigated the activity of phytochemically characterized (HPLC-DAD-MSn) extracts from forsythia leaves and flowers on the pro- and anti-inflammatory functions of leukocytes (effects on IL-1β, IL-8, TNF-α, and TGFβ release) and their adherence to endothelial cells. Using bio-guided fractionation, we isolated the active compounds and determined their biological activity, and we included the positive control quercetin. Methods: The effect on IL-1β, TNF-α, IL-8, and TGF-α production by leukocytes was measured by enzyme-linked immunosorbent assay (ELISA). The surface expression of adhesion molecules was analyzed with flow cytometry, and the neutrophil attachment to the endothelial cells was assessed fluorimetrically. The effects on p38MAPK, ERK1/2 and JNK phosphorylation were determined using western blots. Results: Leaf extracts had the effect of decreasing TNF-α production in neutrophils and monocyte/macrophage cells. The bio-guided fractionation led to the isolation of the following lignan aglycones: (+)-pinoresinol, (+)-epipinoresinol, (−)-matairesinol, (+)-phillygenin, and (−)-arctigenin. Only phillygenin was able to stimulate the anti-inflammatory function of macrophages by inducing TGF-β release and IL-10 receptor surface expression. Arctigenin, phillygenin, and a metabolite produced by the gut microbiota, enterolactone, decreased TNF-α and IL-1β production and neutrophil adhesion to endothelial cells, probably by attenuating the p38 and ERK kinase pathways. Conclusion: Forsythia x intermedia is a valuable source of active lignans, which may be potential candidates for treating inflammatory diseases that are associated with the excessive production of cytokines such as TNF-α and IL-1β.
Barbara Michalak; Agnieszka Filipek; Piotr Chomicki; Małgorzata Pyza; Marta Woźniak; Barbara Żyżyńska-Granica; Jakub Piwowarski; Agnieszka Kicel; Monika Olszewska; Anna K. Kiss. Lignans From Forsythia x Intermedia Leaves and Flowers Attenuate the Pro-inflammatory Function of Leukocytes and Their Interaction With Endothelial Cells. Frontiers in Pharmacology 2018, 9, 1 .
AMA StyleBarbara Michalak, Agnieszka Filipek, Piotr Chomicki, Małgorzata Pyza, Marta Woźniak, Barbara Żyżyńska-Granica, Jakub Piwowarski, Agnieszka Kicel, Monika Olszewska, Anna K. Kiss. Lignans From Forsythia x Intermedia Leaves and Flowers Attenuate the Pro-inflammatory Function of Leukocytes and Their Interaction With Endothelial Cells. Frontiers in Pharmacology. 2018; 9 ():1.
Chicago/Turabian StyleBarbara Michalak; Agnieszka Filipek; Piotr Chomicki; Małgorzata Pyza; Marta Woźniak; Barbara Żyżyńska-Granica; Jakub Piwowarski; Agnieszka Kicel; Monika Olszewska; Anna K. Kiss. 2018. "Lignans From Forsythia x Intermedia Leaves and Flowers Attenuate the Pro-inflammatory Function of Leukocytes and Their Interaction With Endothelial Cells." Frontiers in Pharmacology 9, no. : 1.
Essential oils from flowers and leaves of Grindelia integrifolia DC. were investigated for the first time in terms of chemical composition and antimicrobial activity. The GC-FID/MS analysis allowed for the identification of 58 and 72 volatiles, comprising 92.4 and 90.1% of the oils, respectively. The major components of the flower oil were α-pinene (34.9%) and limonene (13.1%), while myrcene (16.9%), spathulenol (12.3%), β-eudesmol (11.9%) and limonene (10.1%) dominated among the leaf volatiles. The antimicrobial activity, evaluated against 12 selected bacteria and fungus, was found moderate, with the strongest effect of both oils observed against C. albicans (MIC = MBC: 0.63 and 0.31 mg/mL for flower and leaf oil, respectively).
Sławomira Nowak; Paweł Lisiecki; Anna Tomaszczak-Nowak; Ewa Grudzińska; Monika Olszewska; Agnieszka Kicel. Chemical composition and antimicrobial activity of the essential oils from flowers and leaves of Grindelia integrifolia DC. Natural Product Research 2018, 33, 1535 -1540.
AMA StyleSławomira Nowak, Paweł Lisiecki, Anna Tomaszczak-Nowak, Ewa Grudzińska, Monika Olszewska, Agnieszka Kicel. Chemical composition and antimicrobial activity of the essential oils from flowers and leaves of Grindelia integrifolia DC. Natural Product Research. 2018; 33 (10):1535-1540.
Chicago/Turabian StyleSławomira Nowak; Paweł Lisiecki; Anna Tomaszczak-Nowak; Ewa Grudzińska; Monika Olszewska; Agnieszka Kicel. 2018. "Chemical composition and antimicrobial activity of the essential oils from flowers and leaves of Grindelia integrifolia DC." Natural Product Research 33, no. 10: 1535-1540.
Izabela Weremczuk-Jeżyna; Ewa Skała; Monika Olszewska; Anna Kiss; Ewa Balcerczak; Halina Wysokińska; Agnieszka Kicel. The identification and quantitative determination of rosmarinic acid and salvianolic acid B in hairy root cultures of Dracocephalum forrestii W.W. Smith. Industrial Crops and Products 2016, 91, 125 -131.
AMA StyleIzabela Weremczuk-Jeżyna, Ewa Skała, Monika Olszewska, Anna Kiss, Ewa Balcerczak, Halina Wysokińska, Agnieszka Kicel. The identification and quantitative determination of rosmarinic acid and salvianolic acid B in hairy root cultures of Dracocephalum forrestii W.W. Smith. Industrial Crops and Products. 2016; 91 ():125-131.
Chicago/Turabian StyleIzabela Weremczuk-Jeżyna; Ewa Skała; Monika Olszewska; Anna Kiss; Ewa Balcerczak; Halina Wysokińska; Agnieszka Kicel. 2016. "The identification and quantitative determination of rosmarinic acid and salvianolic acid B in hairy root cultures of Dracocephalum forrestii W.W. Smith." Industrial Crops and Products 91, no. : 125-131.
The antioxidant efficiency of 70% aqueous methanolic extracts from the leaves of twelve selected Cotoneaster Medik. species was evaluated using four complementary in vitro tests based on SET- (single electron transfer) and HAT-type (hydrogen atom transfer) mechanisms (DPPH, FRAP, O2•− and H2O2 scavenging assays). The samples exhibited the dose-dependent responses in all assays with activity parameters of EC50 = 18.5–34.5 µg/mL for DPPH; 0.9–3.8 mmol Fe2+/g for FRAP; SC50 = 27.7–74.8 µg/mL for O2•−; and SC50 = 29.0–91.3 µg/mL for H2O2. Significant linear correlations (|r| = 0.76–0.97, p < 0.01) between activity parameters and total contents of phenolics (5.2%–15.4% GAE) and proanthocyanidins (2.1%–15.0% CYE), with weak or no effects for chlorogenic acid isomers (0.69%–2.93%) and total flavonoids (0.28%–1.40%) suggested that among the listed polyphenols, proanthocyanidins are the most important determinants of the tested activity. UHPLC-PDA-ESI-QTOF-MS analyses led to detection of 34 polyphenols, of which 10 B-type procyanidins, 5 caffeoylquinic acids and 14 flavonoids were identified. After cluster analysis of the data matrix, the leaves of Cotoneaster zabelii, C. splendens, C. bullatus, C. divaricatus, C. hjelmqvistii and C. lucidus were selected as the most promising sources of natural antioxidants, exhibiting the highest phenolic levels and antioxidant capacities, and therefore the greatest potential for pharmaceutical applications.
Agnieszka Kicel; Piotr Michel; Aleksandra Owczarek; Anna Marchelak; Dorota Żyżelewicz; Grażyna Budryn; Joanna Oracz; Monika Anna Olszewska. Phenolic Profile and Antioxidant Potential of Leaves from Selected Cotoneaster Medik. Species. Molecules 2016, 21, 688 .
AMA StyleAgnieszka Kicel, Piotr Michel, Aleksandra Owczarek, Anna Marchelak, Dorota Żyżelewicz, Grażyna Budryn, Joanna Oracz, Monika Anna Olszewska. Phenolic Profile and Antioxidant Potential of Leaves from Selected Cotoneaster Medik. Species. Molecules. 2016; 21 (6):688.
Chicago/Turabian StyleAgnieszka Kicel; Piotr Michel; Aleksandra Owczarek; Anna Marchelak; Dorota Żyżelewicz; Grażyna Budryn; Joanna Oracz; Monika Anna Olszewska. 2016. "Phenolic Profile and Antioxidant Potential of Leaves from Selected Cotoneaster Medik. Species." Molecules 21, no. 6: 688.
Agnieszka Kicel; Aleksandra Owczarek; Piotr Michel; Krystyna Skalicka-Woźniak; Anna Kiss; Monika Olszewska. Application of HPCCC, UHPLC-PDA-ESI-MS 3 and HPLC-PDA methods for rapid, one-step preparative separation and quantification of rutin in Forsythia flowers. Industrial Crops and Products 2015, 76, 86 -94.
AMA StyleAgnieszka Kicel, Aleksandra Owczarek, Piotr Michel, Krystyna Skalicka-Woźniak, Anna Kiss, Monika Olszewska. Application of HPCCC, UHPLC-PDA-ESI-MS 3 and HPLC-PDA methods for rapid, one-step preparative separation and quantification of rutin in Forsythia flowers. Industrial Crops and Products. 2015; 76 ():86-94.
Chicago/Turabian StyleAgnieszka Kicel; Aleksandra Owczarek; Piotr Michel; Krystyna Skalicka-Woźniak; Anna Kiss; Monika Olszewska. 2015. "Application of HPCCC, UHPLC-PDA-ESI-MS 3 and HPLC-PDA methods for rapid, one-step preparative separation and quantification of rutin in Forsythia flowers." Industrial Crops and Products 76, no. : 86-94.
This study was designed to evaluate the flavonoid, saponin (TSC) and phenolic (TPC) contents and in vitro antioxidant activity of the crude (CME) and dry extracts and fractions of Medicago lupulina L. aerial parts. A validated RP-HPLC method led to quantitation of flavonols (kaempferol, quercetin and myricetin) and flavones (apigenin and luteolin) in the hydrolyzed extract. TSC and TPC were assayed spectrophotometrically at 560 and 760 nm, respectively. The antioxidant activity of the CME and the dry fractions were followed in vitro by DPPH free radical and ferric reducing antioxidant power (FRAP) methods. The flavonoid content of CME was 1.27 mg/g dw. The prevailing flavonoids were luteolin and myricetin, at concentrations of 0.37 and 0.36 mg/g dw, respectively. TSC and TPC were detected in CME at the level of 90.4 mg ESE/g dw, and 12.9 mg GAE/g dw, respectively. In the DPPH and FRAP tests, the CME exhibited antioxidant capacity with TEAA and FRAP values of 45.4 μmol Trolox®/g dw and 0.2 mmol Fe2+/g dw, respectively. The diethyl ether dry fraction was the most valuable one, showing the highest antioxidant activity (TEAA = 726.1 μmol Trolox®/g dw, FRAP = 2349.4 μmol Fe2+/g dw) that was in accordance with its high TPC (162.4 mg/g dw).
Agnieszka Kicel; Monika Olszewska. Evaluation of Antioxidant Activity, and Quantitative Estimation of Flavonoids, Saponins and Phenols in Crude Extract and Dry Fractions of Medicago lupulina Aerial Parts. Natural Product Communications 2015, 10, 483 -6.
AMA StyleAgnieszka Kicel, Monika Olszewska. Evaluation of Antioxidant Activity, and Quantitative Estimation of Flavonoids, Saponins and Phenols in Crude Extract and Dry Fractions of Medicago lupulina Aerial Parts. Natural Product Communications. 2015; 10 (3):483-6.
Chicago/Turabian StyleAgnieszka Kicel; Monika Olszewska. 2015. "Evaluation of Antioxidant Activity, and Quantitative Estimation of Flavonoids, Saponins and Phenols in Crude Extract and Dry Fractions of Medicago lupulina Aerial Parts." Natural Product Communications 10, no. 3: 483-6.
The aim of the study was to obtain transformed roots ofRhaponticum carthamoidesand evaluate their phytochemical profile. Hairy roots were induced from leaf explants by the transformation ofAgrobacterium rhizogenesstrains A4 and ATCC 15834. The best response (43%) was achieved by infection with A4 strain. The effects of different liquid media (WPM, B5, SH) with full and half-strength concentrations of macro- and micronutrients on biomass accumulation of the best grown hairy root line (RC3) at two different lighting conditions (light or dark) were investigated. The highest biomass (93 g L−1of the fresh weight after 35 days) was obtained in WPM medium under periodic light. UPLC-PDA-ESI-MS3and HPLC-PDA analyses of 80% aqueous methanol extracts from the obtained hairy roots revealed the presence of eleven caffeoylquinic acids and their derivatives and five flavonoid glycosides. The production of caffeoylquinic acids and their derivatives was elevated in hairy roots grown in the light. Only light-grown hairy roots demonstrated the capability for the biosynthesis of such flavonoid glycosides as quercetagetin, quercetin, luteolin, and patuletin hexosides. Chlorogenic acid, 3,5-di-O-caffeoylquinic acid and a tentatively identified tricaffeoylquinic acid derivative were detected as the major compounds present in the transformed roots.
Ewa Skała; Agnieszka Kicel; Monika Olszewska; Anna Kiss; Halina Wysokińska. Establishment of Hairy Root Cultures ofRhaponticum carthamoides(Willd.) Iljin for the Production of Biomass and Caffeic Acid Derivatives. BioMed Research International 2015, 2015, 1 -11.
AMA StyleEwa Skała, Agnieszka Kicel, Monika Olszewska, Anna Kiss, Halina Wysokińska. Establishment of Hairy Root Cultures ofRhaponticum carthamoides(Willd.) Iljin for the Production of Biomass and Caffeic Acid Derivatives. BioMed Research International. 2015; 2015 ():1-11.
Chicago/Turabian StyleEwa Skała; Agnieszka Kicel; Monika Olszewska; Anna Kiss; Halina Wysokińska. 2015. "Establishment of Hairy Root Cultures ofRhaponticum carthamoides(Willd.) Iljin for the Production of Biomass and Caffeic Acid Derivatives." BioMed Research International 2015, no. : 1-11.
Dry leaf extracts of eastern teaberry (Gaultheria procumbens L.) were evaluated as a source of bioactive phytocompounds through systematic activity testing and phytochemical profiling. The antioxidant efficiency was tested using five complementary in vitro models (DPPH; FRAP; linoleic acid (LA) peroxidation assay; O2•− and H2O2 scavenging tests) in parallel with standard antioxidants. The 75% methanol extract and its diethyl ether, ethyl acetate (EAF), n-butanol and water fractions exhibited the dose-dependent responses in all assays, with the highest capacities found for EAF (DPPH EC50 = 2.9 μg/mL; FRAP = 12.8 mmol Fe2+/g; IC50 for LA-peroxidation = 123.9 μg/mL; O2•− SC50 = 3.9 μg/mL; H2O2 SC50 = 7.2 μg/mL). The EAF had also the highest anti-inflammatory activity in the inhibition tests of lipoxygenase and hyaluronidase (60.14% and 21.83% effects, respectively, at the concentration of 100 μg/mL). Activity parameters of the extracts correlated strongly with the levels of total phenolics (72.4–270.7 mg GAE/g), procyanidins, and phenolic acids, whereas for flavonoids only moderate effects were observed. Comprehensive UHPLC-PDA-ESI-MS3 and HPLC-PDA studies led to the identification of 35 polyphenols with a procyanidin A-type trimer, quercetin 3-O-glucuronide, isomers of caffeoylquinic acids, and (‒)-epicatechin being the dominant components. Significant activity levels, high phenolic contents and high extraction yields (39.4%–42.5% DW for defatted and crude methanol extracts, respectively) indicate the value of eastern teaberry leaves as bioactive products.
Piotr Michel; Anna Dobrowolska; Agnieszka Kicel; Aleksandra Owczarek; Agnieszka Bazylko; Sebastian Granica; Jakub P. Piwowarski; Monika A. Olszewska. Polyphenolic Profile, Antioxidant and Anti-Inflammatory Activity of Eastern Teaberry (Gaultheria procumbens L.) Leaf Extracts. Molecules 2014, 19, 20498 -20520.
AMA StylePiotr Michel, Anna Dobrowolska, Agnieszka Kicel, Aleksandra Owczarek, Agnieszka Bazylko, Sebastian Granica, Jakub P. Piwowarski, Monika A. Olszewska. Polyphenolic Profile, Antioxidant and Anti-Inflammatory Activity of Eastern Teaberry (Gaultheria procumbens L.) Leaf Extracts. Molecules. 2014; 19 (12):20498-20520.
Chicago/Turabian StylePiotr Michel; Anna Dobrowolska; Agnieszka Kicel; Aleksandra Owczarek; Agnieszka Bazylko; Sebastian Granica; Jakub P. Piwowarski; Monika A. Olszewska. 2014. "Polyphenolic Profile, Antioxidant and Anti-Inflammatory Activity of Eastern Teaberry (Gaultheria procumbens L.) Leaf Extracts." Molecules 19, no. 12: 20498-20520.
A study of the composition of essential oils from aerial and underground parts of Geum rivale L. and Geum urbanum L. growing in Poland led to the identification of 130 compounds. The main compound of the essential oil from underground parts of G. urbanum was eugenol (69.2%), whereas cis-myrtanal (53.3%) was the major constituent of the essential oil from roots of G. rivale. The essential oils from aerial parts of the plants contained large amounts of aliphatic compounds with ( Z)-3-hexenol (38.4%) being the dominant constituent of the essential oil from aerial parts of G. urbanum and 1-octen-3-ol (33.9%) from G. rivale.
Aleksandra Owczarek; Jan Gudej; Agnieszka Kicel. Composition of Essential Oil from Aerial and Underground Parts of Geum rivale and G. urbanum Growing in Poland. Natural Product Communications 2013, 8, 505 -8.
AMA StyleAleksandra Owczarek, Jan Gudej, Agnieszka Kicel. Composition of Essential Oil from Aerial and Underground Parts of Geum rivale and G. urbanum Growing in Poland. Natural Product Communications. 2013; 8 (4):505-8.
Chicago/Turabian StyleAleksandra Owczarek; Jan Gudej; Agnieszka Kicel. 2013. "Composition of Essential Oil from Aerial and Underground Parts of Geum rivale and G. urbanum Growing in Poland." Natural Product Communications 8, no. 4: 505-8.
In order to facilitate the quality control of Trifolium repens flowers and leaves, a RP-HPLC method with UV detection was developed for the simultaneous quantitative determination of flavonols and isoflavones. The total flavonoid and phenolic (TPC) contents were determined spectrophotometrically in a visible part of the light spectrum at 425 and 760 nm, respectively. Additionally, evaluation of the antioxidant properties of the plant materials was performed using the DPPH in vitro test. The results showed that the flowers are the richest source of phenolics ranging from 28.7 to 38.8 mg GAE/g, and flavonoids, calculated for hyperoside, up to 20 mg HP/g, which hydrolyzed mainly to flavonols (the quercetin level greater than 6 mg/g). T. repens is poor in isoflavones; similar quantities of ca. 0.2 mg/g were detected in the flowers and leaves. The flower and leaf extracts showed antioxidant activity towards DPPH with EC50 values ranging from 72.3 to 179.3 μg/mL. Significant linear correlations were found between antioxidant potentials of the studied plant materials and total phenolic and flavonoid contents determined by HPLC and spectrophotometric methods ( R2 in the range of 0.97 - 0.99).
Agnieszka Kicel; Maria Wolbiś. Phenolic Content and DPPH Radical Scavenging Activity of the Flowers and Leaves of Trifolium repens. Natural Product Communications 2013, 8, 99 -102.
AMA StyleAgnieszka Kicel, Maria Wolbiś. Phenolic Content and DPPH Radical Scavenging Activity of the Flowers and Leaves of Trifolium repens. Natural Product Communications. 2013; 8 (1):99-102.
Chicago/Turabian StyleAgnieszka Kicel; Maria Wolbiś. 2013. "Phenolic Content and DPPH Radical Scavenging Activity of the Flowers and Leaves of Trifolium repens." Natural Product Communications 8, no. 1: 99-102.
Agnieszka Kicel; Maria Wolbis. Coumarins from the flowers of Trifolium repens. Chemistry of Natural Compounds 2012, 48, 130 -132.
AMA StyleAgnieszka Kicel, Maria Wolbis. Coumarins from the flowers of Trifolium repens. Chemistry of Natural Compounds. 2012; 48 (1):130-132.
Chicago/Turabian StyleAgnieszka Kicel; Maria Wolbis. 2012. "Coumarins from the flowers of Trifolium repens." Chemistry of Natural Compounds 48, no. 1: 130-132.
The flowers and leaves of Trifolium repens L. (Fabaceae) were subjected to phytochemical investigation in order to identify their major chemical constituents and to evaluate in vitro antioxidant activity of the isolated compounds against DPPH˙. A total of 12 flavonoids, pterocarpan and methyl caffeate were isolated, then characterised by UV, MS, NMR spectroscopy and identified as quercetin and kaempferol 3-O-(6″-α-rhamnopyranosyl-2″-β-xylopyranosyl)-β-galactopyranosides (1, 2), kaempferol 3-O-(2″,6″-α-dirhamnopyranosyl)-β-galactopyranoside, mauritianin (3), quercetin and kaempferol 3-O-(2″-β-xylopyranosyl)-β-galactopyranosides (4, 5), kaempferol and quercetin 3-O-β-(6″-O-acetyl)-galactopyranosides (6, 7), trifolin (8), hyperoside (9), myricetin 3-O-β-galactopyranoside (10), quercetin (11), ononin (12), medicarpin 3-O-β-glucopyranoside (13) and methyl caffeate (14). Mauritianin, ononin, pterocarpan and methyl caffeate have been reported in this plant for the first time. The compounds 4, 7, 9, 10, and 11 were tested for their antioxidant effect against DPPH˙. All studied compounds were found to have potent activity, but the most effective in the test were compounds 9, 10 and 11 (EC50 values in the range 7.51–9.52 µM).
Agnieszka Kicel; Maria Wolbiś. Study on the phenolic constituents of the flowers and leaves of Trifolium repens L. Natural Product Research 2011, 26, 2050 -2054.
AMA StyleAgnieszka Kicel, Maria Wolbiś. Study on the phenolic constituents of the flowers and leaves of Trifolium repens L. Natural Product Research. 2011; 26 (21):2050-2054.
Chicago/Turabian StyleAgnieszka Kicel; Maria Wolbiś. 2011. "Study on the phenolic constituents of the flowers and leaves of Trifolium repens L." Natural Product Research 26, no. 21: 2050-2054.
In order to find new sources of natural antioxidants, the antioxidant potential of 70% methanolic extracts from the inflorescences and leaves of 16 species from the genus Sorbus s.s. was evaluated using two complementary in vitro test systems: the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical-scavenging assay and the AAPH [2,2¢-azobis-(2-amidinopropane)dihydrochloride]-induced linoleic acid (LA) peroxidation test. The radical-scavenging capacities of the extracts towards the DPPH radical were in the range of 0.25-0.86 millimolar Trolox® equivalents/g dry weight. They were significantly correlated (r = -0.8089, p Sorbus extracts to be universal antioxidants. Significant linear correlations were also found between the different antioxidant potentials and total phenolic contents as estimated by the Folin-Ciocalteu method and further verified by serial determinations of proanthocyanidins, chlorogenic acid isomers and flavonoids (çrêin the range of 0.71-0.95, p S. aucuparia, S. pohuashanensis, S. decora, S. koehneana, S. commixta, S. gracilis, and S. sitchensis, and the leaves of S. wilfordii, S. pogonopetala, and S. gracilis) exhibiting the highest antioxidant activity and total phenolic levels and therefore the greatest potential as effective sources for natural health products.
Monika A. Olszewska; Sławomira Nowak; Piotr Michel; Piotr Banaszczak; Agnieszka Kicel. Assessment of the Content of Phenolics and Antioxidant Action of Inflorescences and Leaves of Selected Species from the Genus Sorbus Sensu Stricto. Molecules 2010, 15, 8769 -8783.
AMA StyleMonika A. Olszewska, Sławomira Nowak, Piotr Michel, Piotr Banaszczak, Agnieszka Kicel. Assessment of the Content of Phenolics and Antioxidant Action of Inflorescences and Leaves of Selected Species from the Genus Sorbus Sensu Stricto. Molecules. 2010; 15 (12):8769-8783.
Chicago/Turabian StyleMonika A. Olszewska; Sławomira Nowak; Piotr Michel; Piotr Banaszczak; Agnieszka Kicel. 2010. "Assessment of the Content of Phenolics and Antioxidant Action of Inflorescences and Leaves of Selected Species from the Genus Sorbus Sensu Stricto." Molecules 15, no. 12: 8769-8783.