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Monika A. Olszewska
Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Lodz, 90-151 Lodz, Poland

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Journal article
Published: 22 June 2021 in Antioxidants
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The bark of Aesculus hippocastanum is an herbal remedy used in conditions connected with vascular insufficiency; however, there is a lack of data concerning its mechanisms of action. The present work is a preliminary investigation into some of the potential directions of the bark activity. The phytochemically (qualitative UHPLC-PDA-MS/MS and quantitative UHPLC-PDA assays) characterized extract and its four main constituents (esculin, fraxin, (‒)-epicatechin and procyanidin A2) were first evaluated in terms of their antioxidant capacity. All analytes demonstrated dose-dependent scavenging potential towards the most common in vivo oxidants, with particularly advantageous capacity of the extract and its flavan-3-ol constituents against peroxynitrite (3.37–13.26 mmol AA/g), hydroxyl radical (5.03–8.91 mmol AA/g) and superoxide radical (3.50–5.50 mmol AA/g). Moreover, even at low concentrations (1–5 µg/mL), they protected components of human plasma against oxidative damage inflicted by peroxynitrite, preventing oxidation of plasma protein thiols and diminishing the tyrosine nitration and lipid peroxidation. High efficiency of the analytes was also demonstrated in preventing the peroxynitrite-induced nitrative changes of fibrinogen (up to 80% inhibition for (‒)-epicatechin at 50 µg/mL), an important protein of coagulation cascade. Additionally, the extract and its constituents had, at most, moderate inhibitory activity towards platelet aggregation induced by ADP and only negligible influence on clotting times. The results show that, among the investigated properties, the antioxidant activity might, to the highest extent, be responsible for the bark efficacy in vascular disorders, thus supporting its application in those conditions; they also indicate the directions for future research that would allow for better understanding of the bark activity.

ACS Style

Aleksandra Owczarek; Joanna Kolodziejczyk-Czepas; Joanna Woźniak-Serwata; Anna Magiera; Natalia Kobiela; Katarzyna Wąsowicz; Monika Olszewska. Potential Activity Mechanisms of Aesculus hippocastanum Bark: Antioxidant Effects in Chemical and Biological In Vitro Models. Antioxidants 2021, 10, 995 .

AMA Style

Aleksandra Owczarek, Joanna Kolodziejczyk-Czepas, Joanna Woźniak-Serwata, Anna Magiera, Natalia Kobiela, Katarzyna Wąsowicz, Monika Olszewska. Potential Activity Mechanisms of Aesculus hippocastanum Bark: Antioxidant Effects in Chemical and Biological In Vitro Models. Antioxidants. 2021; 10 (7):995.

Chicago/Turabian Style

Aleksandra Owczarek; Joanna Kolodziejczyk-Czepas; Joanna Woźniak-Serwata; Anna Magiera; Natalia Kobiela; Katarzyna Wąsowicz; Monika Olszewska. 2021. "Potential Activity Mechanisms of Aesculus hippocastanum Bark: Antioxidant Effects in Chemical and Biological In Vitro Models." Antioxidants 10, no. 7: 995.

Journal article
Published: 09 April 2021 in Antioxidants
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Oxidative post-translational modifications of fibrinogen (a multifunctional blood plasma protein essential for hemostasis) are associated with the pathogenesis of cardiovascular disorders (CVDs). Prunus spinosa flower is a herbal medicine used in an adjuvant treatment of CVDs and rich in polyphenolic antioxidants. In the present study, phytochemically standardized P. spinosa flower extracts, their primary native polyphenols and potential phenolic metabolites were evaluated in vitro for their protective effects on fibrinogen (isolated and in the human plasma matrix) using a panel of complementary methods (SDS-PAGE, western blot, C-ELISA, fluorometry, FRAP, TBARS). The results revealed that the tested analytes at in vivo relevant levels (1–5 µg/mL) considerably reduced the structural changes in the fibrinogen molecule under the oxidative stress conditions induced by peroxynitrite. In particular, they diminished the oxidation and/or nitration of amino acid residues, including tyrosine and tryptophan, as well as the formation of high molecular weight aggregates. The decrease in the levels of 3-nitrotyrosine was about 13.5–33.0% and 58.3–97.1% at 1 µg/mL and 50 µg/mL, respectively. The study indicated that low molecular weight polyphenols were crucial for the protective activity of the extracts toward fibrinogen and other human plasma components. The investigated model compounds effectively protected total plasma proteins and lipids against oxidative damage (by reducing the levels of 3-nitrotyrosine and thiobarbituric acid-reactive substances and normalizing/enhancing the non-enzymatic antioxidant capacity of plasma). The work provides insight into the role of native and metabolized polyphenols as contributory factors to the systemic activity of blackthorn flower extracts within the circulatory system.

ACS Style

Anna Marchelak; Joanna Kolodziejczyk-Czepas; Paulina Wasielewska; Pawel Nowak; Monika Olszewska. The Effects of Prunus spinosa L. Flower Extracts, Model Polyphenols and Phenolic Metabolites on Oxidative/Nitrative Modifications of Human Plasma Components with Particular Emphasis on Fibrinogen In Vitro. Antioxidants 2021, 10, 581 .

AMA Style

Anna Marchelak, Joanna Kolodziejczyk-Czepas, Paulina Wasielewska, Pawel Nowak, Monika Olszewska. The Effects of Prunus spinosa L. Flower Extracts, Model Polyphenols and Phenolic Metabolites on Oxidative/Nitrative Modifications of Human Plasma Components with Particular Emphasis on Fibrinogen In Vitro. Antioxidants. 2021; 10 (4):581.

Chicago/Turabian Style

Anna Marchelak; Joanna Kolodziejczyk-Czepas; Paulina Wasielewska; Pawel Nowak; Monika Olszewska. 2021. "The Effects of Prunus spinosa L. Flower Extracts, Model Polyphenols and Phenolic Metabolites on Oxidative/Nitrative Modifications of Human Plasma Components with Particular Emphasis on Fibrinogen In Vitro." Antioxidants 10, no. 4: 581.

Journal article
Published: 03 December 2020 in Metabolites
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Plants have been used for medical purposes since ancient times. However, a detailed analysis of their biological properties and their associated active compounds is needed to justify their therapeutic use in modern medicine. The aim of the study was to identify and quantify the phenolics present in hydromethanolic extracts of the roots and shoots of the Chinese Salvia species, Salvia bulleyana. The qualitative and quantitative analyses were carried out by ultrahigh-performance liquid chromatography with electrospray ionization mass spectrometry detection (UHPLC-PDA-ESI-MS), and high-performance liquid chromatography with photodiode array (HPLC-PDA) detection. The extracts of S. bulleyana were also screened for their antioxidant activity using ferric ion (Fe3+) reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) cation (ABTS), superoxide radical anion (O2•–), and inhibition of lipid peroxidation assays. The S. bulleyana extracts were found to contain 38 substances, of which 36 were phenols, with a total level of 14.4 mg/g DW (dry weight) in shoots, and 23.1 mg/g DW in roots. Twenty-eight phenols were polyphenolic acids or their derivatives, the most abundant in shoots being rosmarinic acid, and in roots, salvianolic acid K followed by rosmarinic acid. The other major phenolic acids were caffeic acid, caffeoyl-threonic acids, isomers of lithospermic acid, salvianolic acid F, salvianolic acid B, and yunnaneic acid E. In addition to polyphenolic acids, nine flavonoids were detected in the shoot extract. While both extracts showed significant antioxidant activity, the shoot extract, containing both polyphenolic acids and flavonoids, demonstrated a slightly greater antioxidant potential in some of the anti-radical tests than the roots. However, the root extract proved to be slightly more effective in the lipid peroxidation inhibition test. Thus, S. bulleyana was demonstrated as a promising source of antioxidants, and worthy of further more detailed studies.

ACS Style

Izabela Grzegorczyk-Karolak; Marta Krzemińska; Anna K. Kiss; Monika A. Olszewska; Aleksandra Owczarek. Phytochemical Profile and Antioxidant Activity of Aerial and Underground Parts of Salvia bulleyana Diels. Plants. Metabolites 2020, 10, 497 .

AMA Style

Izabela Grzegorczyk-Karolak, Marta Krzemińska, Anna K. Kiss, Monika A. Olszewska, Aleksandra Owczarek. Phytochemical Profile and Antioxidant Activity of Aerial and Underground Parts of Salvia bulleyana Diels. Plants. Metabolites. 2020; 10 (12):497.

Chicago/Turabian Style

Izabela Grzegorczyk-Karolak; Marta Krzemińska; Anna K. Kiss; Monika A. Olszewska; Aleksandra Owczarek. 2020. "Phytochemical Profile and Antioxidant Activity of Aerial and Underground Parts of Salvia bulleyana Diels. Plants." Metabolites 10, no. 12: 497.

Paper
Published: 19 August 2020 in Food & Function
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The fruits of Gaultheria procumbens are the richest known dietary source of salicylates with confirmed anti-inflammatory potential.

ACS Style

Piotr Michel; Sebastian Granica; Karolina Rosińska; Jarosław Rojek; Łukasz Poraj; Monika Anna Olszewska. Biological and chemical insight into Gaultheria procumbens fruits: a rich source of anti-inflammatory and antioxidant salicylate glycosides and procyanidins for food and functional application. Food & Function 2020, 11, 7532 -7544.

AMA Style

Piotr Michel, Sebastian Granica, Karolina Rosińska, Jarosław Rojek, Łukasz Poraj, Monika Anna Olszewska. Biological and chemical insight into Gaultheria procumbens fruits: a rich source of anti-inflammatory and antioxidant salicylate glycosides and procyanidins for food and functional application. Food & Function. 2020; 11 (9):7532-7544.

Chicago/Turabian Style

Piotr Michel; Sebastian Granica; Karolina Rosińska; Jarosław Rojek; Łukasz Poraj; Monika Anna Olszewska. 2020. "Biological and chemical insight into Gaultheria procumbens fruits: a rich source of anti-inflammatory and antioxidant salicylate glycosides and procyanidins for food and functional application." Food & Function 11, no. 9: 7532-7544.

Journal article
Published: 19 August 2020 in Industrial Crops and Products
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Sorbus domestica L. leaves are a phenylpropanoid-rich herbal medicine with antioxidant and anti-inflammatory activity. Here, to optimise harvesting time of the leaves for medicinal application, seasonal variability in the expression of genes encoding enzymes involved in phenylpropanoid biosynthesis (qRT-PCR, quantitative real time polymerase chain reaction) and its relationship with changes in the phytochemical profile (UHPLC-PDA-ESI-MS3, HPLC-PDA-fingerprint, spectrophotometric methods) and antioxidant properties (2,2-diphenyl-1-picrylhydrazyl scavenging and ferric reducing antioxidant power) was evaluated during foliar development. Significant quantitative differences in polyphenolic composition were revealed between spring leaves (predominance of phenylpropanoid acids and flavonoids) and those harvested during the rest of the growing season (predominance of proanthocyanidins, major active components of S. domestica), while their qualitative composition was stable. A set of 40 compounds were detected in the samples by LC–MS/MS including 38 phenylpropanoids. The total phenylpropanoid level (HPLC) in dried leaves dropped from 25.7 mg/g in May to 20.7 mg/g in October but proanthocyanidins and the antioxidant capacity increased simultaneously up to 12.7 mg/g and 49.7 mmol Trolox equivalents/100 g, respectively. Based on the correlation between gene expression and total phenolics and proanthocyanidins (r > 0.825, p < 0.05), phenylalanine ammonia lyase (PAL) and 4-coumaroyl:CoA ligase (4CL) appeared to be core regulators of the phenolic profile among analysed genes (PAL; 4CL; C4H, cinnamate 4-hydroxylase; CHS, chalcone synthase; F3H, flavanone 3-hydroxylase; FLS, flavonols synthase). Due to the synergy of individual components, variability in activity parameters was relatively moderate. Considering the phenylpropanoid levels and antioxidant capacity, the late summer and autumn months (August-October) might be the most advantageous time for harvesting leaf material of optimal quality.

ACS Style

Magdalena Rutkowska; Ewa Balcerczak; Rafał Świechowski; Monika Dubicka; Monika A. Olszewska. Seasonal variation in phenylpropanoid biosynthesis and in vitro antioxidant activity of Sorbus domestica leaves: Harvesting time optimisation for medicinal application. Industrial Crops and Products 2020, 156, 112858 .

AMA Style

Magdalena Rutkowska, Ewa Balcerczak, Rafał Świechowski, Monika Dubicka, Monika A. Olszewska. Seasonal variation in phenylpropanoid biosynthesis and in vitro antioxidant activity of Sorbus domestica leaves: Harvesting time optimisation for medicinal application. Industrial Crops and Products. 2020; 156 ():112858.

Chicago/Turabian Style

Magdalena Rutkowska; Ewa Balcerczak; Rafał Świechowski; Monika Dubicka; Monika A. Olszewska. 2020. "Seasonal variation in phenylpropanoid biosynthesis and in vitro antioxidant activity of Sorbus domestica leaves: Harvesting time optimisation for medicinal application." Industrial Crops and Products 156, no. : 112858.

Journals
Published: 07 July 2020 in Food & Function
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Broccoli sprout extract rich in sinapic acid derivatives prevents oxidative changes in human plasma and modulates inflammatory response in PBMCs.

ACS Style

Monika Anna Olszewska; Sebastian Granica; Joanna Kolodziejczyk-Czepas; Anna Magiera; Monika Ewa Czerwińska; Pawel Nowak; Magdalena Rutkowska; Piotr Wasiński; Aleksandra Owczarek. Variability of sinapic acid derivatives during germination and their contribution to antioxidant and anti-inflammatory effects of broccoli sprouts on human plasma and human peripheral blood mononuclear cells. Food & Function 2020, 11, 7231 -7244.

AMA Style

Monika Anna Olszewska, Sebastian Granica, Joanna Kolodziejczyk-Czepas, Anna Magiera, Monika Ewa Czerwińska, Pawel Nowak, Magdalena Rutkowska, Piotr Wasiński, Aleksandra Owczarek. Variability of sinapic acid derivatives during germination and their contribution to antioxidant and anti-inflammatory effects of broccoli sprouts on human plasma and human peripheral blood mononuclear cells. Food & Function. 2020; 11 (8):7231-7244.

Chicago/Turabian Style

Monika Anna Olszewska; Sebastian Granica; Joanna Kolodziejczyk-Czepas; Anna Magiera; Monika Ewa Czerwińska; Pawel Nowak; Magdalena Rutkowska; Piotr Wasiński; Aleksandra Owczarek. 2020. "Variability of sinapic acid derivatives during germination and their contribution to antioxidant and anti-inflammatory effects of broccoli sprouts on human plasma and human peripheral blood mononuclear cells." Food & Function 11, no. 8: 7231-7244.

Journal article
Published: 06 May 2020 in Journal of Biotechnology
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This study was to obtain stable transformed roots of Salvia bulleyana using A. rhizogenes strain A4 and then evaluate their phytochemical profile and selected the most productive clone. Our results indicated that the type of explant and medium used for bacterium and explant incubation had an influence on the frequency of hairy root formation. The best response was obtained on leaves infected with bacteria cultivated on YMB medium supplemented with acetosyringone. Of the four selected transformed root clones, after five-week cultivation in Woody Plant (WP) medium, the highest growth indexes were demonstrated for line C1: i.e. 13 for fresh and 15 for dry weight (81.4 and 8.2 g/l fresh and dry weight, respectively). The qualitative analysis of hydromethanolic extracts of hairy roots of S. bulleyana using UPLC-PDA-ESI-MS/MS method showed the presence of 10 polyphenolic compounds including predominant rosmarinic acid (RA), its derivatives (hexoside and methyl rosmarinate), caffeic acid, its derivatives and several salvianolic acids: K, E and F. Their production varied among the four root clones studied; the highest RA (39.6 mg/g dry weight) and total polyphenol (48.9 mg/g dry weight) level were found in the roots of C4 clone. These values were significantly higher than those of the roots of plants grown for several years under field conditions. The transformation of the obtained root cultures was confirmed by polymerase chain reaction using aux1, aux2, rolB, rolC and rolD primers.

ACS Style

Marta Wojciechowska; Aleksandra Owczarek; Anna K. Kiss; Renata Grąbkowska; Monika A. Olszewska; Izabela Grzegorczyk-Karolak. Establishment of hairy root cultures of Salvia bulleyana Diels for production of polyphenolic compounds. Journal of Biotechnology 2020, 318, 10 -19.

AMA Style

Marta Wojciechowska, Aleksandra Owczarek, Anna K. Kiss, Renata Grąbkowska, Monika A. Olszewska, Izabela Grzegorczyk-Karolak. Establishment of hairy root cultures of Salvia bulleyana Diels for production of polyphenolic compounds. Journal of Biotechnology. 2020; 318 ():10-19.

Chicago/Turabian Style

Marta Wojciechowska; Aleksandra Owczarek; Anna K. Kiss; Renata Grąbkowska; Monika A. Olszewska; Izabela Grzegorczyk-Karolak. 2020. "Establishment of hairy root cultures of Salvia bulleyana Diels for production of polyphenolic compounds." Journal of Biotechnology 318, no. : 10-19.

Data article
Published: 22 February 2020 in Data in Brief
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This paper presents data on the optimization and validation of an RP-HPLC-PDA method for quantification of 30 phenolic constituents of the blackthorn (Prunus spinosa L.) flower. The method development data cover detailed descriptions of the optimization process in terms of elution solvents, gradient profile, temperature, and flow rate. The validation data cover accuracy and precision (intra- and inter-day variability) for retention times and peak areas. Moreover, the quantification data for the commercial samples of blackthorn flower (different manufactures and years of collection), as well as for the extracts (of different polarity) prepared thereof, are included. The data presented here were related to the article: “Simultaneous quantification of thirty polyphenols in blackthorn flowers and dry extracts prepared thereof: HPLC-PDA method development and validation for quality control” [1].

ACS Style

Anna Marchelak; Monika Olszewska; Aleksandra Owczarek. Data on the optimization and validation of HPLC-PDA method for quantification of thirty polyphenols in blackthorn flowers and dry extracts prepared thereof. Data in Brief 2020, 29, 105319 .

AMA Style

Anna Marchelak, Monika Olszewska, Aleksandra Owczarek. Data on the optimization and validation of HPLC-PDA method for quantification of thirty polyphenols in blackthorn flowers and dry extracts prepared thereof. Data in Brief. 2020; 29 ():105319.

Chicago/Turabian Style

Anna Marchelak; Monika Olszewska; Aleksandra Owczarek. 2020. "Data on the optimization and validation of HPLC-PDA method for quantification of thirty polyphenols in blackthorn flowers and dry extracts prepared thereof." Data in Brief 29, no. : 105319.

Journal article
Published: 21 January 2020 in Journal of Pharmaceutical and Biomedical Analysis
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The paper presents development and validation of a RP-HPLC-PDA method for quantification of 30 phenolic constituents of the blackthorn (Prunus spinosa L.) flower, a traditional European herbal medicine with a unique and complex composition. The target analytes were selected from over 50 active compounds present in the investigated plant material, and their separation was optimized on a C18 Ascentis Express fused-core column (2.7 μm, 150 mm × 4.6 mm), in a step-by-step process, in terms of elution solvents, gradient profile, temperature, and flow rate. The final procedure was carried out with an acetonitrile-tetrahydrofuran gradient at a flow rate of 1.09 mL/min and column temperature of 28°C. Under those conditions, the matrix peaks were satisfactorily separated within 35 min. The validation showed good precision (RSD < 5 %), accuracy (93.5–102.1 %), linearity (r > 0.9998), and sensitivity (LODs 0.51–2.05 ng) of the method. The real sample analysis demonstrated its applicability for quantification of the phenolics both in commercial samples of P. spinosa flowers (different manufacturers and years of collection), as well as in the extracts (of different polarity) prepared thereof. Thus, the developed procedure proved to be a useful tool in quality control, and the optimization approach might serve as a practical guideline for LC-method development in complex matrices.

ACS Style

Anna Marchelak; Monika Olszewska; Aleksandra Owczarek. Simultaneous quantification of thirty polyphenols in blackthorn flowers and dry extracts prepared thereof: HPLC-PDA method development and validation for quality control. Journal of Pharmaceutical and Biomedical Analysis 2020, 184, 113121 .

AMA Style

Anna Marchelak, Monika Olszewska, Aleksandra Owczarek. Simultaneous quantification of thirty polyphenols in blackthorn flowers and dry extracts prepared thereof: HPLC-PDA method development and validation for quality control. Journal of Pharmaceutical and Biomedical Analysis. 2020; 184 ():113121.

Chicago/Turabian Style

Anna Marchelak; Monika Olszewska; Aleksandra Owczarek. 2020. "Simultaneous quantification of thirty polyphenols in blackthorn flowers and dry extracts prepared thereof: HPLC-PDA method development and validation for quality control." Journal of Pharmaceutical and Biomedical Analysis 184, no. : 113121.

Journal article
Published: 12 January 2020 in Antioxidants
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Cotoneaster plants are sources of traditional medicines and dietary products, with health benefits resulting from their phenolic contents and antioxidant activity. In this work, active markers of the leaves of C. bullatus and C. zabelii were characterized and evaluated in an integrated phytochemical and biological activity study. Based on UHPLC-PDA-ESI-MS3 analysis, twelve analytes were preselected from the constituents of the hydromethanolic leaf extracts, and two of them—caffeoylmalic acid and quercetin 3--O-β-d-(2″--O-β-d-xylopyranosyl)galactopyranoside (QPH)—were isolated for full identification (NMR spectroscopy: 1H, 13C, COSY, HMBC, HMQC). All selected phenolics contributed to the antioxidant activity of the extracts, which was demonstrated in chemical in vitro tests (DPPH, FRAP, and TBARS) and in a biological model of human plasma exposed to oxidative/nitrative stress induced by peroxynitrite. This contribution was partly due to the synergy between individual polyphenols, evidenced by an isobolographic analysis of the interactions of (–)-epicatechin, chlorogenic acid, and QPH as representatives of three classes of Cotoneaster polyphenols. All twelve markers, including also neochlorogenic acid, cryptochlorogenic acid, procyanidin B2, procyanidin C1, rutin, hyperoside, isoquercitrin, and quercitrin, were thus applied as calibration standards, and a fast, accurate, reproducible, and fully validated RP-HPLC-PDA method for quality control and standardization of the target extracts was proposed.

ACS Style

Agnieszka Kicel; Aleksandra Owczarek; Paulina Kapusta; Joanna Kolodziejczyk-Czepas; Monika A. Olszewska. Contribution of Individual Polyphenols to Antioxidant Activity of Cotoneaster bullatus and Cotoneaster zabelii Leaves—Structural Relationships, Synergy Effects and Application for Quality Control. Antioxidants 2020, 9, 69 .

AMA Style

Agnieszka Kicel, Aleksandra Owczarek, Paulina Kapusta, Joanna Kolodziejczyk-Czepas, Monika A. Olszewska. Contribution of Individual Polyphenols to Antioxidant Activity of Cotoneaster bullatus and Cotoneaster zabelii Leaves—Structural Relationships, Synergy Effects and Application for Quality Control. Antioxidants. 2020; 9 (1):69.

Chicago/Turabian Style

Agnieszka Kicel; Aleksandra Owczarek; Paulina Kapusta; Joanna Kolodziejczyk-Czepas; Monika A. Olszewska. 2020. "Contribution of Individual Polyphenols to Antioxidant Activity of Cotoneaster bullatus and Cotoneaster zabelii Leaves—Structural Relationships, Synergy Effects and Application for Quality Control." Antioxidants 9, no. 1: 69.

Short communication
Published: 19 September 2019 in Natural Product Research
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Chloroform extracts from leaves, inflorescences and fruits of Prunus padus were analysed for anti-inflammatory activity and accumulation of corosolic (CA), ursolic (UA) and oleanolic (OA) acids. The analytes were identified and quantified by GC-MS and UHPLC-PDA. Their total levels depend on plant material type and harvesting time, and varied from 0.25 mg/g DW in fruits, through 0.76–1.09 mg/g DW in flowers, to 1.41–4.54 mg/g DW in leaves. Significant variation in the leaf analytes contents was observed during vegetation with the peak amounts in autumn, which indicated the optimal harvesting season. The plant extracts inhibited pro-inflammatory enzymes (lipoxygenase and hyaluronidase) in a concentration-dependent manner, and their activity parameters correlated with the levels and activity of pure triterpene acids, especially CA and UA. The results of the comparison with the positive controls (heparin, indomethacin, dexamethasone) might partly support the application of P. padus in anti-inflammatory therapies, reported by traditional medicine.

ACS Style

Anna Magiera; Anna Marchelak; Piotr Michel; Aleksandra Owczarek; Monika A. Olszewska. Lipophilic extracts from leaves, inflorescences and fruits of Prunus padus L. as potential sources of corosolic, ursolic and oleanolic acids with anti-inflammatory activity. Natural Product Research 2019, 35, 2263 -2268.

AMA Style

Anna Magiera, Anna Marchelak, Piotr Michel, Aleksandra Owczarek, Monika A. Olszewska. Lipophilic extracts from leaves, inflorescences and fruits of Prunus padus L. as potential sources of corosolic, ursolic and oleanolic acids with anti-inflammatory activity. Natural Product Research. 2019; 35 (13):2263-2268.

Chicago/Turabian Style

Anna Magiera; Anna Marchelak; Piotr Michel; Aleksandra Owczarek; Monika A. Olszewska. 2019. "Lipophilic extracts from leaves, inflorescences and fruits of Prunus padus L. as potential sources of corosolic, ursolic and oleanolic acids with anti-inflammatory activity." Natural Product Research 35, no. 13: 2263-2268.

Journal article
Published: 20 June 2019 in Molecules
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Sorbus domestica leaves are a traditionally used herbal medicine recommended for the treatment of oxidative stress-related diseases. Dry leaf extracts (standardized by LC-MS/MS and LC-PDA) and nine model activity markers (polyphenols), were tested in scavenging assays towards six in vivo-relevant oxidants (O2•−, OH•, NO•, H2O2, ONOO−, HClO). Ascorbic acid (AA) and Trolox (TX) were used as positive standards. The most active extracts were the diethyl ether and ethyl acetate fractions with activities in the range of 3.61–20.03 µmol AA equivalents/mg, depending on the assay. Among the model compounds, flavonoids were especially effective in OH• scavenging, while flavan-3-ols were superior in O2•− quenching. The most active constituents were quercetin, (−)-epicatechin, procyanidins B2 and C1 (3.94–24.16 µmol AA/mg), but considering their content in the extracts, isoquercitrin, (−)-epicatechin and chlorogenic acid were indicated as having the greatest influence on extract activity. The analysis of the synergistic effects between those three compounds in an O2•− scavenging assay demonstrated that the combination of chlorogenic acid and isoquercitrin exerts the greatest influence. The results indicate that the extracts possess a strong and broad spectrum of antioxidant capacity and that their complex composition plays a key role, with various constituents acting complementarily and synergistically.

ACS Style

Magdalena Rutkowska; Monika Anna Olszewska; Joanna Kolodziejczyk-Czepas; Pawel Nowak; Aleksandra Owczarek. Sorbus domestica Leaf Extracts and Their Activity Markers: Antioxidant Potential and Synergy Effects in Scavenging Assays of Multiple Oxidants. Molecules 2019, 24, 2289 .

AMA Style

Magdalena Rutkowska, Monika Anna Olszewska, Joanna Kolodziejczyk-Czepas, Pawel Nowak, Aleksandra Owczarek. Sorbus domestica Leaf Extracts and Their Activity Markers: Antioxidant Potential and Synergy Effects in Scavenging Assays of Multiple Oxidants. Molecules. 2019; 24 (12):2289.

Chicago/Turabian Style

Magdalena Rutkowska; Monika Anna Olszewska; Joanna Kolodziejczyk-Czepas; Pawel Nowak; Aleksandra Owczarek. 2019. "Sorbus domestica Leaf Extracts and Their Activity Markers: Antioxidant Potential and Synergy Effects in Scavenging Assays of Multiple Oxidants." Molecules 24, no. 12: 2289.

Journal article
Published: 09 April 2019 in International Journal of Molecular Sciences
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Salicylate-rich plants are an attractive alternative to synthetic anti-inflammatory drugs due to a better safety profile and the advantage of complementary anti-inflammatory and antioxidant effects of the co-occurring non-salicylate phytochemicals. Here, the phytochemical value and biological effects in vitro and ex vivo of the stems of one of such plants, Gaultheria procumbens L., were evaluated. The best extrahent for effective recovery of the active stem molecules was established in comparative studies of five extracts. The UHPLC-PDA-ESI-MS3, HPLC-PDA, and UV-photometric assays revealed that the selected acetone extract (AE) accumulates a rich polyphenolic fraction (35 identified constituents; total content 427.2 mg/g dw), mainly flavanols (catechins and proanthocyanidins; 201.3 mg/g dw) and methyl salicylate glycosides (199.9 mg/g dw). The extract and its model components were effective cyclooxygenase-2, lipoxygenase, and hyaluronidase inhibitors; exhibited strong antioxidant capacity in six non-cellular in vitro models (AE and procyanidins); and also significantly and dose-dependently reduced the levels of reactive oxygen species (ROS), and the release of cytokines (IL-1β, IL-8, TNF-α) and proteinases (elastase-2, metalloproteinase-9) in human neutrophils stimulated ex vivo by lipopolysaccharide (LPS) and N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP). The cellular safety of AE was demonstrated by flow cytometry. The results support the application of the plant in traditional medicine and encourage the use of AE for development of new therapeutic agents.

ACS Style

Piotr Michel; Sebastian Granica; Anna Magiera; Karolina Rosińska; Małgorzata Jurek; Łukasz Poraj; Monika Anna Olszewska. Salicylate and Procyanidin-Rich Stem Extracts of Gaultheria procumbens L. Inhibit Pro-Inflammatory Enzymes and Suppress Pro-Inflammatory and Pro-Oxidant Functions of Human Neutrophils Ex Vivo. International Journal of Molecular Sciences 2019, 20, 1753 .

AMA Style

Piotr Michel, Sebastian Granica, Anna Magiera, Karolina Rosińska, Małgorzata Jurek, Łukasz Poraj, Monika Anna Olszewska. Salicylate and Procyanidin-Rich Stem Extracts of Gaultheria procumbens L. Inhibit Pro-Inflammatory Enzymes and Suppress Pro-Inflammatory and Pro-Oxidant Functions of Human Neutrophils Ex Vivo. International Journal of Molecular Sciences. 2019; 20 (7):1753.

Chicago/Turabian Style

Piotr Michel; Sebastian Granica; Anna Magiera; Karolina Rosińska; Małgorzata Jurek; Łukasz Poraj; Monika Anna Olszewska. 2019. "Salicylate and Procyanidin-Rich Stem Extracts of Gaultheria procumbens L. Inhibit Pro-Inflammatory Enzymes and Suppress Pro-Inflammatory and Pro-Oxidant Functions of Human Neutrophils Ex Vivo." International Journal of Molecular Sciences 20, no. 7: 1753.

Journal article
Published: 01 April 2019 in Phytochemistry Letters
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Anna Marchelak; Aleksandra Owczarek; Magdalena Rutkowska; Piotr Michel; Joanna Kolodziejczyk-Czepas; Pawel Nowak; Monika Anna Olszewska. New insights into antioxidant activity of Prunus spinosa flowers: Extracts, model polyphenols and their phenolic metabolites in plasma towards multiple in vivo-relevant oxidants. Phytochemistry Letters 2019, 30, 288 -295.

AMA Style

Anna Marchelak, Aleksandra Owczarek, Magdalena Rutkowska, Piotr Michel, Joanna Kolodziejczyk-Czepas, Pawel Nowak, Monika Anna Olszewska. New insights into antioxidant activity of Prunus spinosa flowers: Extracts, model polyphenols and their phenolic metabolites in plasma towards multiple in vivo-relevant oxidants. Phytochemistry Letters. 2019; 30 ():288-295.

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Anna Marchelak; Aleksandra Owczarek; Magdalena Rutkowska; Piotr Michel; Joanna Kolodziejczyk-Czepas; Pawel Nowak; Monika Anna Olszewska. 2019. "New insights into antioxidant activity of Prunus spinosa flowers: Extracts, model polyphenols and their phenolic metabolites in plasma towards multiple in vivo-relevant oxidants." Phytochemistry Letters 30, no. : 288-295.

Short communication
Published: 05 March 2019 in Phytochemistry Letters
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Complementary UHPLC-PDA-ESI-MS3, HPLC-PDA-fingerprint, Folin-Ciocalteau, n-butanol/HCl and hide powder assays were employed to study the qualitative and quantitative differences in phenolic composition between different organs (leaves, flowers, bark and fruits) of Cotoneaster integerrimus. The bark and flowers were the richest source of polyphenols (107.0–113.6 mg GAE/g dw of plant material), with the dominating proanthocyanidins (96.3–97.7 mg/g dw), and low-molecular weight flavan-3-ols (21.5–22.0 mg/g). Among the leaf phenolics (93.6 mg/g), caffeoylquinic pseudodepsides (21.0 mg/g) and flavonol glycosides (21.7 mg/g) were prevailing, while the fruits contained relatively low amounts of polyphenols (49.5 mg/g) with high proportion of procyanidins (27.1 mg/g). In in vitro tests of antioxidant activity (DPPH, FRAP and TBARS), the effects of bark, flowers and leaves were significantly higher in comparison to these of the fruits. The bark sample had also the highest anti-inflammatory activity in the inhibition tests towards lipoxygenase and hyaluronidase. Despite the differences, the composition of the investigated organs seems consistent with the indications of traditional medicine and supports their therapeutic application in inflammation/infectious diseases and cardiovascular disorders.

ACS Style

Agnieszka Kicel; Aleksandra Owczarek; Paulina Gralak; Pawel Ciszewski; Monika A. Olszewska. Polyphenolic profile, antioxidant activity, and pro-inflammatory enzymes inhibition of leaves, flowers, bark and fruits of Cotoneaster integerrimus: A comparative study. Phytochemistry Letters 2019, 30, 349 -355.

AMA Style

Agnieszka Kicel, Aleksandra Owczarek, Paulina Gralak, Pawel Ciszewski, Monika A. Olszewska. Polyphenolic profile, antioxidant activity, and pro-inflammatory enzymes inhibition of leaves, flowers, bark and fruits of Cotoneaster integerrimus: A comparative study. Phytochemistry Letters. 2019; 30 ():349-355.

Chicago/Turabian Style

Agnieszka Kicel; Aleksandra Owczarek; Paulina Gralak; Pawel Ciszewski; Monika A. Olszewska. 2019. "Polyphenolic profile, antioxidant activity, and pro-inflammatory enzymes inhibition of leaves, flowers, bark and fruits of Cotoneaster integerrimus: A comparative study." Phytochemistry Letters 30, no. : 349-355.

Journal article
Published: 28 February 2019 in Acta Poloniae Pharmaceutica - Drug Research
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Anna Magiera; Monika Sienkiewicz; Monika Olszewska; Agnieszka Kicel; Piotr Michel. Chemical profile and antibacterial activity of essential oils from leaves and fruits of Gaultheria procumbens L. cultivated in Poland. Acta Poloniae Pharmaceutica - Drug Research 2019, 76, 93 -102.

AMA Style

Anna Magiera, Monika Sienkiewicz, Monika Olszewska, Agnieszka Kicel, Piotr Michel. Chemical profile and antibacterial activity of essential oils from leaves and fruits of Gaultheria procumbens L. cultivated in Poland. Acta Poloniae Pharmaceutica - Drug Research. 2019; 76 (1):93-102.

Chicago/Turabian Style

Anna Magiera; Monika Sienkiewicz; Monika Olszewska; Agnieszka Kicel; Piotr Michel. 2019. "Chemical profile and antibacterial activity of essential oils from leaves and fruits of Gaultheria procumbens L. cultivated in Poland." Acta Poloniae Pharmaceutica - Drug Research 76, no. 1: 93-102.

Short communication
Published: 11 February 2019 in Phytochemistry Letters
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This work is aimed at establishing analytical and bioactivity markers for the quality control of polyphenol-rich extracts of Sorbus domestica leaves – an antioxidant and anti-inflammatory agent. The potential marker analytes, representative of the main groups of S. domestica polyphenols, were pre-selected by LC–MS/MS analysis of the methanol-water (7:3, v/v) leaf extract (MED). For structural identification, two of them (rare flavonoids) were isolated from MED by preparative HPLC and analysed by 1D and 2D NMR (COSY, HMBC, HMQC) spectroscopy. The in vitro tests revealed that all analytes increase the non-enzymatic antioxidant capacity of plasma, effectively protect human plasma components against oxidative/nitrative damage induced by peroxynitrite (reduce the levels of thiobarbituric acid-reactive substances and 3-nitrotyrosine), and inhibit lipoxygenase and hyaluronidase. Considering the activity parameters and concentration levels, the highest contribution (70–80%) to the activity of the total phenolic fraction of MED was attributed to flavanols represented by (–)-epicatechin, procyanidin B2 and procyanidin C1. However, taking into account the observed synergistic effects, flavonoid and phenolic acid fractions (represented by chlorogenic acid, quercitrin, rutin, quercetin and its 3-O-(2′'-O-β-d-glucopyranosyl)-α-l-rhamnopyranoside and 3-O-(2′'-O-β-d-xylopyranosyl)-α-l-rhamnopyranoside) had also a substantial impact. All nine markers were thus applied as calibration standards, and a simple, accurate, reproducible and fully validated RP-HPLC-PDA method for the standardisation of the target extracts was proposed.

ACS Style

Magdalena Rutkowska; Aleksandra Owczarek; Joanna Kolodziejczyk-Czepas; Piotr Michel; Dorota Gabriela Piotrowska; Paulina Kapusta; Pawel Nowak; Monika Olszewska. Identification of bioactivity markers of Sorbus domestica leaves in chromatographic, spectroscopic and biological capacity tests: Application for the quality control. Phytochemistry Letters 2019, 30, 278 -287.

AMA Style

Magdalena Rutkowska, Aleksandra Owczarek, Joanna Kolodziejczyk-Czepas, Piotr Michel, Dorota Gabriela Piotrowska, Paulina Kapusta, Pawel Nowak, Monika Olszewska. Identification of bioactivity markers of Sorbus domestica leaves in chromatographic, spectroscopic and biological capacity tests: Application for the quality control. Phytochemistry Letters. 2019; 30 ():278-287.

Chicago/Turabian Style

Magdalena Rutkowska; Aleksandra Owczarek; Joanna Kolodziejczyk-Czepas; Piotr Michel; Dorota Gabriela Piotrowska; Paulina Kapusta; Pawel Nowak; Monika Olszewska. 2019. "Identification of bioactivity markers of Sorbus domestica leaves in chromatographic, spectroscopic and biological capacity tests: Application for the quality control." Phytochemistry Letters 30, no. : 278-287.

Research article
Published: 04 February 2019 in Oxidative Medicine and Cellular Longevity
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Polyphenol-rich plant extracts might alleviate the negative impact of oxidative stress and inflammation, but careful phytochemical standardisation and evaluation of various mechanisms are required to fully understand their effects. In this context, flower extracts of Sorbus aucuparia L.—a traditional medicinal plant—were investigated in the present work. The LC-MS/MS profiling of the extracts, obtained by fractionated extraction, led to the identification of 66 constituents, mostly flavonols (quercetin and sexangularetin glycosides with dominating isoquercitrin), pseudodepsides of quinic and shikimic acids (prevailing isomers of chlorogenic acid and cynarin), and flavanols (catechins and proanthocyanidins). Minor extract components of possible chemotaxonomic value were flavalignans (cinchonain I isomers) and phenylamides (spermidine derivatives). As assessed by HPLC-PDA and UV-spectrophotometric studies, the extracts were polyphenol-abundant, with the contents up to 597.6 mg/g dry weight (dw), 333.9 mg/g dw, 382.0 mg/g dw, and 169.0 mg/g dw of total phenolics, flavonoids, proanthocyanidins, and caffeoylquinic acids, respectively. Their biological in vitro effects were phenolic-dependent and the strongest for diethyl ether, ethyl acetate, and n-butanol fractions of the methanol-water (7 : 3, v/v) extract. The extracts showed significant, concentration-dependent ability to scavenge in vivo-relevant radical/oxidant agents (O2∙−, OH∙, H2O2, ONOO–, NO∙, and HClO) with the strongest effects towards OH∙, ONOO–, HClO, and O2∙− (compared to ascorbic acid). Moreover, the extracts efficiently inhibited lipoxygenase and hyaluronidase (compared to indomethacin) but were inactive towards xanthine oxidase. At in vivo-relevant levels (1-5 μg/mL), they also effectively protected human plasma components (proteins and lipids) against ONOO–-induced oxidative damage (reduced the levels of 3-nitrotyrosine, lipid hydroperoxides, and thiobarbituric acid-reactive substances) and normalised/enhanced the total nonenzymatic antioxidant capacity of plasma. In cytotoxicity tests, the extracts did not affect the viability of human PBMCs and might be regarded as safe. The results support the application of the extracts in the treatment of oxidative stress-related pathologies cross-linked with inflammatory changes.

ACS Style

Monika A. Olszewska; Joanna Kolodziejczyk-Czepas; Magdalena Rutkowska; Anna Magiera; Piotr Michel; Marcin W. Rejman; Pawel Nowak; Aleksandra Owczarek. The Effect of Standardised Flower Extracts of Sorbus aucuparia L. on Proinflammatory Enzymes, Multiple Oxidants, and Oxidative/Nitrative Damage of Human Plasma Components In Vitro. Oxidative Medicine and Cellular Longevity 2019, 2019, 1 -18.

AMA Style

Monika A. Olszewska, Joanna Kolodziejczyk-Czepas, Magdalena Rutkowska, Anna Magiera, Piotr Michel, Marcin W. Rejman, Pawel Nowak, Aleksandra Owczarek. The Effect of Standardised Flower Extracts of Sorbus aucuparia L. on Proinflammatory Enzymes, Multiple Oxidants, and Oxidative/Nitrative Damage of Human Plasma Components In Vitro. Oxidative Medicine and Cellular Longevity. 2019; 2019 ():1-18.

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Monika A. Olszewska; Joanna Kolodziejczyk-Czepas; Magdalena Rutkowska; Anna Magiera; Piotr Michel; Marcin W. Rejman; Pawel Nowak; Aleksandra Owczarek. 2019. "The Effect of Standardised Flower Extracts of Sorbus aucuparia L. on Proinflammatory Enzymes, Multiple Oxidants, and Oxidative/Nitrative Damage of Human Plasma Components In Vitro." Oxidative Medicine and Cellular Longevity 2019, no. : 1-18.

Journal article
Published: 13 December 2018 in Industrial Crops and Products
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Rhaponticum carthamoides (Willd.) Iljin (Asteraceae) is an endemic plant species whose roots and rhizomes have been used for many years in traditional Siberian medicine. In this study, the transformed roots of R. carthamoides obtained after Agrobacterium rhizogenes transformation were able to spontaneously regenerate green buds and shoots. Bud and shoot regeneration was observed in more than 90% of the cultures grown in Schenk and Hildebrandt (SH) or Gamborg (B5) liquid medium with full micro- and macronutrient concentrations, under light conditions. The regeneration of shoots from transformed roots may be improved by supplementation of Murashige and Skoog (MS) agar medium with indole-3-acetic acid (IAA) and benzyladenine (BA). The shoots were multiplied on MS medium supplemented with 0.1 mg/L IAA and 0.2 mg/L BA and then rooted successfully. The transformation of the plants derived from transformed roots was confirmed by PCR analysis and Southern hybridization. The three-month-old pRi-transformed plants (TR-plants) were able to biosynthesize caffeoylquinic acid derivatives such as chlorogenic acid, and 3,5- or 4,5-di-O-caffeoylquinic acids. Plants obtained directly from seeds (SD-plants) being in the same age and grown under the same conditions as the transformed plants were used as compared material. The transformed plants demonstrated higher secondary metabolite levels and greater biomass accumulation than SD-plants. It was found that extracts from TR-plants exhibited antioxidant properties in the model of human blood plasma and inhibited the growth of A2058 human melanoma cells depending on the tested concentration. The present study demonstrated that the pRi-transformed plants of R. carthamoides may be an alternative to conventional field crops and these plants can be used as the promising source of the valuable compounds, such as caffeoylquinic acid derivatives.

ACS Style

Ewa Skała; Laurent Picot; Michał Bijak; Joanna Saluk-Bijak; Janusz Szemraj; Agnieszka Kicel; Monika A. Olszewska; Przemysław Sitarek. An efficient plant regeneration from Rhaponticum carthamoides transformed roots, enhanced caffeoylquinic acid derivatives production in pRi-transformed plants and their biological activity. Industrial Crops and Products 2018, 129, 327 -338.

AMA Style

Ewa Skała, Laurent Picot, Michał Bijak, Joanna Saluk-Bijak, Janusz Szemraj, Agnieszka Kicel, Monika A. Olszewska, Przemysław Sitarek. An efficient plant regeneration from Rhaponticum carthamoides transformed roots, enhanced caffeoylquinic acid derivatives production in pRi-transformed plants and their biological activity. Industrial Crops and Products. 2018; 129 ():327-338.

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Ewa Skała; Laurent Picot; Michał Bijak; Joanna Saluk-Bijak; Janusz Szemraj; Agnieszka Kicel; Monika A. Olszewska; Przemysław Sitarek. 2018. "An efficient plant regeneration from Rhaponticum carthamoides transformed roots, enhanced caffeoylquinic acid derivatives production in pRi-transformed plants and their biological activity." Industrial Crops and Products 129, no. : 327-338.

Research article
Published: 24 October 2018 in Oxidative Medicine and Cellular Longevity
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The work presents the results of an investigation into the molecular background of the activity of Cotoneaster fruits, providing a detailed description of their phytochemical composition and some of the mechanisms of their anti-inflammatory and antioxidant effects. GS-FID-MS and UHPLC-PDA-ESI-MS3 methods were applied to identify the potentially health-beneficial constituents of lipophilic and hydrophilic fractions, leading to the identification of fourteen unsaturated fatty acids (with dominant linoleic acid, 375.4–1690.2 mg/100 g dw), three phytosterols (with dominant β-sitosterol, 132.2–463.3 mg/100 g), two triterpenoid acids (10.9–54.5 mg/100 g), and twenty-six polyphenols (26.0–43.5 mg GAE/g dw). The most promising polyphenolic fractions exhibited dose-dependent anti-inflammatory activity in in vitro tests of lipoxygenase (IC50 in the range of 7.7–24.9 μg/U) and hyaluronidase (IC50 in the range of 16.4–29.3 μg/U) inhibition. They were also demonstrated to be a source of effective antioxidants, both in in vitro chemical tests (DPPH, FRAP, and TBARS) and in a biological model, in which at in vivo-relevant levels (1–5 μg/mL) they normalized/enhanced the nonenzymatic antioxidant capacity of human plasma and efficiently protected protein and lipid components of plasma against peroxynitrite-induced oxidative/nitrative damage. Moreover, the investigated extracts did not exhibit cytotoxicity towards human PMBCs. Among the nine Cotoneaster species tested, C. hjelmqvistii, C. zabelii, C. splendens, and C. bullatus possess the highest bioactive potential and might be recommended as dietary and functional food products.

ACS Style

Agnieszka Kicel; Joanna Kolodziejczyk-Czepas; Aleksandra Owczarek; Magdalena Rutkowska; Anna Wajs-Bonikowska; Sebastian Granica; Pawel Nowak; Monika A. Olszewska. Multifunctional Phytocompounds in Cotoneaster Fruits: Phytochemical Profiling, Cellular Safety, Anti-Inflammatory and Antioxidant Effects in Chemical and Human Plasma Models In Vitro. Oxidative Medicine and Cellular Longevity 2018, 2018, 1 -16.

AMA Style

Agnieszka Kicel, Joanna Kolodziejczyk-Czepas, Aleksandra Owczarek, Magdalena Rutkowska, Anna Wajs-Bonikowska, Sebastian Granica, Pawel Nowak, Monika A. Olszewska. Multifunctional Phytocompounds in Cotoneaster Fruits: Phytochemical Profiling, Cellular Safety, Anti-Inflammatory and Antioxidant Effects in Chemical and Human Plasma Models In Vitro. Oxidative Medicine and Cellular Longevity. 2018; 2018 ():1-16.

Chicago/Turabian Style

Agnieszka Kicel; Joanna Kolodziejczyk-Czepas; Aleksandra Owczarek; Magdalena Rutkowska; Anna Wajs-Bonikowska; Sebastian Granica; Pawel Nowak; Monika A. Olszewska. 2018. "Multifunctional Phytocompounds in Cotoneaster Fruits: Phytochemical Profiling, Cellular Safety, Anti-Inflammatory and Antioxidant Effects in Chemical and Human Plasma Models In Vitro." Oxidative Medicine and Cellular Longevity 2018, no. : 1-16.