Tick-borne diseases pose a serious threat to human health in South-Eastern Europe, including Kosovo. While Crimean–Congo hemorrhagic fever (CCHF) is a well-known emerging infection in this area, there are no accurate data on Lyme borreliosis and tick-borne encephalitis (TBE). Therefore, we sampled and tested 795 ticks. Ixodes ricinus (n = 218), Dermacentor marginatus (n = 98), and Haemaphysalis spp. (n = 24) were collected from the environment by flagging (all from Kosovo), while Hyalomma marginatum (n = 199 from Kosovo, all from Kosovo) and Rhipicephalus bursa (n = 130, 126 from Albania) could be collected only by removal from animal pasture and domestic ruminants. Ticks were collected in the years 2014/2015 and tested for viral RNA of CCHF and TBE viruses, as well as for DNA of Borrelia burgdorferi sensu lato by real-time PCR. In Kosovo, nine ticks were positive for RNA of Crimean–Congo hemorrhagic fever virus and seven for DNA of B. burgdorferi s. l. None of the ticks tested positive for TBEV. CCHF virus was detected in one H. marginatum male specimen collected while feeding on grazing cattle from the Prizren region and in eight R. bursa specimens (five females and three males collected while feeding on grazing sheep and cattle) from the Prishtina region (Kosovo). B. burgdorferi s. l. was detected in seven questing ticks (four male and one female D. marginatus, two I. ricinus one female and one male) from the Mitrovica region (Kosovo). Our study confirmed that CCHF virus is circulating in Kosovo mainly in H. marginatum and R. bursa in the central areas of the country. B. burgdorferi s. l. was found in its major European host tick, I. ricinus, but also in D. marginatus, in the north of the Kosovo. In order to prevent the spread of these diseases and better control of the tick-borne infections, an improved vector surveillance and testing of ticks for the presence of pathogens needs to be established.

Implementing biosecurity measures in the pig production is crucial to optimize animal health and reduce antimicrobial usage. A legal framework may help to ensure all stakeholders understand the need of biosecurity measures and to implement them correctly. Limited knowledge is available about how implementation of biosecurity measures is regulated in European countries. Therefore, the aim of this study was to identify which biosecurity measures were mandatory by national legislation and/or by pig sector organizations of European countries for the intensive pig production under normal circumstances (i.e. no (threat of) disease outbreaks). A questionnaire including 51 biosecurity measures, 32 on external and 19 on internal, was developed and sent to national country representatives. Twenty-four European countries, of which 18 belonging to the European Union, indicated which biosecurity measures were mandatory by law. Four countries, France, Spain, Italy and Romania had a single national pig-specific biosecurity legislation, while in the other countries multiple legislations had to be consulted to complete the questionnaire. In general, there was a high heterogeneity in the number of measures that were mandatory by law per country. Most biosecurity measures addressed in the national legislation focused on external biosecurity and were measures that can be easily audited when conducting a farm visit such as the presence of a hygiene lock, fencing around the farm and the existence of documentation. A significant (p < 0.001) positive correlation was observed between the presence of external and internal biosecurity measures in the legislation. Yet, no significant association was observed between the number of biosecurity measures present in the national legislation and the size of the pig production. Fourteen countries indicated which biosecurity measures were required by pig sector organizations for intensive pig farms. The median number of biosecurity measures mandatory by pig sector organizations but not by law was 13 with a minimum of one measure in Germany and a maximum of 24 in Croatia. Different countries apply different strategies to increase the level of biosecurity. Further research is needed to investigate the impact of the different strategies on the implementation of biosecurity on intensive pig farms in the countries.

Biosecurity is fundamental to animal health, public health, and the economic resilience of livestock systems; however, farm-level adoption remains uneven across regions. Knowledge gaps, language and financial constraints, and limited communication competence among veterinary advisers impede implementation, especially on small- and medium-scale farms. Behavior change-oriented interventions, such as Motivational Interviewing (which deploys multiple specific behavior change techniques as defined in BCTTv1), offer promise but are seldom embedded in veterinary curricula. This study proposes a concept and key elements for biosecurity training. It highlights a modular, evidence-based training framework developed under the COST Action CA20103 “BETTER” (2021–2025), aimed at improving biosecurity understanding and implementation by veterinarians and farmers. The initiative convened European experts to co-design a flexible curriculum that addresses both technical and behavioral challenges using participatory methods and interdisciplinary expertise. The resulting framework consists of five progressive modules: (1) Introduction, (2) Behavior Change and Communication, (3) Disease Transmission & Risk Assessment, (4) Emergency Response & Clinical Biosecurity, and (5) On-Farm Practices. These modules are designed to be combined in a “pick-and-choose” format to match local needs, target audiences and resources. Delivery blends online micro-lessons, participatory workshops, peer networks, and low-cost on-farm demonstrations, while materials are culturally and linguistically adapted and framed in terms of clear economic benefits. Continuous feedback loops encourage iterative refinement and habit formation during the learning process. The proposed training framework seeks to transform biosecurity from a prescriptive doctrine into a farmer-owned daily routine by integrating technical content with behavioral science and context-specific delivery.

This study was aimed for the evaluation of somatic cell count (SCC), physicochemical, and microbiological parameters during the end of lactation in the raw milk of Alpine and native Red goat breed. In the experiment, 102 milk samples from Alpine and native Red goats were included. Two different groups within the same breed were analyzed: a group consisting of animals in their first lactation and the second group consisting of animals from the fifth lactation. The milk samples were individually and daily collected during late lactation for three consecutive weeks, and milk fat, protein, lactose, SCC, and total bacteria with enterobacteria were assessed. Fresh milk of goats from late lactation period had a number of somatic cells (SC) within the expected value with log10 of 5.8–6.18 cells/ml for the compared groups. In both breeds, the total mesophilic bacteria were fewer in numbers, however, in the native Red goat, a larger population of such bacteria was enumerated. The number of coliforms and enterobacteria was below 100 cfu/ml. In the current study, we were able to show a significant difference among the studied breeds depending on lactation and season for fat (p = 0.002), but not for lactose and protein content. A positive correlation for total protein (TP), lactose, and fat as well as for lactose and SCC was found in the native Red goat breed. In the Alpine goat breed, a strong positive correlation (0.821^**) was found for lactose and enterobacteria count (EC). In conclusion, these findings evaluate different goat milk parameters during late lactation period and provide an indirect measure to monitor goat mammary gland health for both breeds.

Livestock farm biosecurity is crucial for animal health and economic sustainability, however uneven adoption/implementation across diverse livestock species and production systems persists. To improve uptake of biosecurity, it is necessary to identify critical economic behavioural, and systematic barriers, and to outline practical drivers. Perceived high costs, labour/time burdens, and uncertain benefits can suppress private investment, while poorly designed indemnities can create moral hazard. Conversely, targeted subsidies, risk-based insurance, and market standards (e.g., certification and procurement) can incentivise implementation. Knowledge and trust gaps, especially in smallholder and backyard settings, further limit compliance. Participatory, and context-specific training led by field veterinarians consistently outperforms top–down messaging, with effective element including: simple, low-cost “easy wins”, tiered checklists, and decision-support tools to help embed routines and demonstrate the value of biosecurity. Integrating clear cost–benefit evidence, incentive-based tools, and co-designed training can transform biosecurity from a perceived practical and cost burden into a resilient, profitable practice that delivers public-good benefits for animal health, trade, and One Health across Europe and beyond.

The health of dairy cows is an important factor affecting the profitability of dairy farms worldwide, and lameness is regarded as one of the most costly dairy cattle diseases. The aim of this study was to estimate the economic cost of cow lameness among Kosovo dairy farms. Data collected from 56 dairy farms were analysed with a farm-level stochastic (Monte Carlo) simulation model to estimate the cost of lameness. Lameness-associated sources of economic loss examined within the model included: reduced milk production, treatment cost, discarded milk, reduced cow body weight, and premature culling. Results showed that prevalence of lameness among cows on Kosovo dairy farms ranged from 17% to 39%. The average annual cost of lameness was estimated at €338.57 per farm (or €46.25 per cow). Reduced milk production was the largest financial contribution to the cost of lameness (45% of total economic loss) followed by premature culling (31% of total economic loss). Discarded milk, reduced cow body weight, and cost of treatment each contributed approximately 8% to the total economic loss. These findings indicate that dairy farmers need to be more cognizant of the financial losses associated with lameness and should be encouraged to implement management strategies to reduce lameness as a means of enhancing farm profitability.