This page has only limited features, please log in for full access.
This study aims to determine the prevalence of STEC in she-camels suffering from mastitis in semi-arid regions by using traditional culture methods and then confirming it with Serological and molecular techniques in milk samples, camel feces, as well as human stool samples for human contacts. In addition, an antibiotic susceptibility profile for these isolates was investigation. Mastitic milk samples were taken after California Mastitis Test (CMT) procedure, and fecal samples were taken from she-camels and human stool samples, then cultured using traditional methods to isolate Escherichiacoli. These isolates were initially classified serologically, then an mPCR (Multiplex PCR) was used to determine virulence genes. Finally, both camel and human isolates were tested for antibiotic susceptibility. Out of a total of 180 she-camels, 34 (18.9%) were mastitic (8.3% clinical and 10.6% sub-clinical mastitis), where it was higher in camels bred with other animals. The total presence of E. coli was 21.9, 13.9, and 33.7% in milk, camel feces, and human stool, respectively, whereas the occurrence of STEC from the total E. coli isolates were 36, 16, and 31.4% for milk, camel feces, and stool, respectively. Among the camel isolates, stx1 was the most frequently detected virulence gene, while hlyA was not detected. The most detected virulence gene in human isolates was stx2 (45.5%), followed by stx1. Camel STEC showed resistance to Oxytetracycline only, while human STEC showed multiple drug resistance to Amoxicillin, Gentamycin, and Clindamycin with 81.8, 72.7, and 63.6%, respectively. Breeding camels in semi-arid areas separately from other animals may reduce the risk of infection with some bacteria, including E. coli; in contrast, mixed breeding with other animals contributes a significant risk factor for STEC emergence in camels.
Mohamed Said Diab; Reda Tarabees; Yasser F. Elnaker; Ghada A. Hadad; Marwa A. Saad; Salah A. Galbat; Sarah Albogami; Aziza M. Hassan; Mahmoud A. O. Dawood; Sabah Ibrahim Shaaban. Molecular Detection, Serotyping, and Antibiotic Resistance of Shiga Toxigenic Escherichia coli Isolated from She-Camels and In-Contact Humans in Egypt. Antibiotics 2021, 10, 1021 .
AMA StyleMohamed Said Diab, Reda Tarabees, Yasser F. Elnaker, Ghada A. Hadad, Marwa A. Saad, Salah A. Galbat, Sarah Albogami, Aziza M. Hassan, Mahmoud A. O. Dawood, Sabah Ibrahim Shaaban. Molecular Detection, Serotyping, and Antibiotic Resistance of Shiga Toxigenic Escherichia coli Isolated from She-Camels and In-Contact Humans in Egypt. Antibiotics. 2021; 10 (8):1021.
Chicago/Turabian StyleMohamed Said Diab; Reda Tarabees; Yasser F. Elnaker; Ghada A. Hadad; Marwa A. Saad; Salah A. Galbat; Sarah Albogami; Aziza M. Hassan; Mahmoud A. O. Dawood; Sabah Ibrahim Shaaban. 2021. "Molecular Detection, Serotyping, and Antibiotic Resistance of Shiga Toxigenic Escherichia coli Isolated from She-Camels and In-Contact Humans in Egypt." Antibiotics 10, no. 8: 1021.
Dietary cinnamon has several bioactive compounds with growth-promoting and immunomodulation potential and is suggested for finfish species. This study evaluated the inclusion of cinnamon at 0, 10, 15, and 20 g/kg in European sea bass (Dicentrarchus labrax) diets. After 90 days, the highest final weight, weight gain, specific growth rate, protein efficiency ratio, and the lowest feed conversion ratio were seen in fish treated with 10 g/kg (p< 0.05). Further, the measured growth hormone in the blood indicated that fish treated with 10 g/kg had a higher level than fish 0 and 20 g/kg. After the feeding trial, fish treated with cinnamon at varying levels had higher lipid content than fish before the feeding trial (p< 0.05). Lower Vibrio spp. and Faecal Coliform counts were observed in fish treated with cinnamon than fish fed a cinnamon-free diet (p< 0.05). The hematocrit level was markedly (p< 0.05) increased in fish fed cinnamon at 10 g/kg compared to the control without significant differences with fish fed 15 and 20 g/kg. Hemoglobin was significantly increased in fish treated with cinnamon at 10, 15, and 20 g/kg compared to fish fed a cinnamon-free diet (p< 0.05). Red and white blood cells (RBCs and WBCs) were meaningfully (p< 0.05) increased in fish treated with cinnamon compared with the control. Markedly, fish treated with cinnamon had higher serum total lipids than the control with the highest value in fish treated with 15 g/kg (p< 0.05). The lysozyme activity was markedly higher in fish treated with 15 g cinnamon/kg than fish fed 0, 10, and 20 g/kg (p< 0.05). Moreover, phagocytic activity was significantly higher in fish treated with cinnamon at 10, and 15 g/kg than fish fed 0 and 20 g/kg (p< 0.05). In conclusion, dietary cinnamon is suggested at 10–15 g/kg for achieving the high production and wellbeing of European sea bass.
Mahmoud Habiba; Ebtehal Hussein; Ahmed Ashry; Ahmed El-Zayat; Aziza Hassan; Ahmed El-Shehawi; Hani Sewilam; Hien Van Doan; Mahmoud Dawood. Dietary Cinnamon Successfully Enhanced the Growth Performance, Growth Hormone, Antibacterial Capacity, and Immunity of European Sea Bass (Dicentrarchus labrax). Animals 2021, 11, 2128 .
AMA StyleMahmoud Habiba, Ebtehal Hussein, Ahmed Ashry, Ahmed El-Zayat, Aziza Hassan, Ahmed El-Shehawi, Hani Sewilam, Hien Van Doan, Mahmoud Dawood. Dietary Cinnamon Successfully Enhanced the Growth Performance, Growth Hormone, Antibacterial Capacity, and Immunity of European Sea Bass (Dicentrarchus labrax). Animals. 2021; 11 (7):2128.
Chicago/Turabian StyleMahmoud Habiba; Ebtehal Hussein; Ahmed Ashry; Ahmed El-Zayat; Aziza Hassan; Ahmed El-Shehawi; Hani Sewilam; Hien Van Doan; Mahmoud Dawood. 2021. "Dietary Cinnamon Successfully Enhanced the Growth Performance, Growth Hormone, Antibacterial Capacity, and Immunity of European Sea Bass (Dicentrarchus labrax)." Animals 11, no. 7: 2128.
Cancer is one of the most serious public health issues worldwide, ranking second only to cardiovascular diseases as a cause of death. Numerous plant extracts have extraordinary health benefits and have been used for centuries to treat a variety of ailments with few side effects. Olive leaves have a long history of medicinal and therapeutic use. In this study, the anti-cancer properties of an olive leaf extract were investigated in vitro using colorectal and prostate cancer cell lines (HT29 and PC3, respectively). A high-performance liquid chromatography analysis showed that the olive leaf extract contained a high chlorogenic acid content. Accordingly, chlorogenic acid may be related to the observed effects of the aqueous extract on cancer cells, including increased inhibition of cancer cell growth, migration, DNA fragmentation, cell cycle arrest at the S phase, reactive oxygen species (ROS) production, and altered gene expression. The effects of the extracts were greater in HT29 than in PC3 cells. These results suggest that chlorogenic acid, the main constituent in the olive extract, is a promising new anti-cancer agent. Further analyses should focus on its in vivo effects on colorectal tumor models, both alone and in combination with established agents.
Sarah Albogami; Aziza Hassan. Assessment of the Efficacy of Olive Leaf (Olea europaea L.) Extracts in the Treatment of Colorectal Cancer and Prostate Cancer Using In Vitro Cell Models. Molecules 2021, 26, 4069 .
AMA StyleSarah Albogami, Aziza Hassan. Assessment of the Efficacy of Olive Leaf (Olea europaea L.) Extracts in the Treatment of Colorectal Cancer and Prostate Cancer Using In Vitro Cell Models. Molecules. 2021; 26 (13):4069.
Chicago/Turabian StyleSarah Albogami; Aziza Hassan. 2021. "Assessment of the Efficacy of Olive Leaf (Olea europaea L.) Extracts in the Treatment of Colorectal Cancer and Prostate Cancer Using In Vitro Cell Models." Molecules 26, no. 13: 4069.
The need to replace antibiotics in aquafeed is increasing, and alternative safe substances are now encouraged for sustainable aquaculture activity. Curcumin is regarded as a multifunctional feed additive with growth-promoting and immunostimulant potential. Thus, this study evaluated dietary inclusion of curcumin at rates of 0, 1.5, 2, 2.5, and 3% in the diets of Gilthead seabream for 150 days. The results showed an improved final body weight, weight gain, specific growth rate, and feed conversion ratio in fish treated with curcumin, in a dose-dependent manner. The highest growth performance was observed in fish fed a diet supplemented with 3% curcumin. The results also showed lowered activity of pathogenic bacteria (Vibrio spp. and Faecal coliform) in the intestines of Gilthead seabream fed a diet with curcumin inclusion, in a dose-dependent manner. The hematological indices were within the normal range for healthy fish, without meaningful effects except for hematocrit, hemoglobin, red blood cells (RBCs), and white blood cells (WBCs), which were markedly increased by dietary curcumin. Phagocytic activity was obviously enhanced by dietary curcumin, compared with the control. The biochemical blood metabolites related to liver function (alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT)), renal tissue (urea), and total cholesterol were within the normal values, without significant differences. Overall, the inclusion of curcumin at a rate of 2–3% improved the growth performance and well-being of Gilthead seabream.
Ahmed Ashry; Aziza Hassan; Mahmoud Habiba; Ahmed El-Zayat; Mohamed El-Sharnouby; Hani Sewilam; Mahmoud Dawood. The Impact of Dietary Curcumin on the Growth Performance, Intestinal Antibacterial Capacity, and Haemato-Biochemical Parameters of Gilthead Seabream (Sparus aurata). Animals 2021, 11, 1779 .
AMA StyleAhmed Ashry, Aziza Hassan, Mahmoud Habiba, Ahmed El-Zayat, Mohamed El-Sharnouby, Hani Sewilam, Mahmoud Dawood. The Impact of Dietary Curcumin on the Growth Performance, Intestinal Antibacterial Capacity, and Haemato-Biochemical Parameters of Gilthead Seabream (Sparus aurata). Animals. 2021; 11 (6):1779.
Chicago/Turabian StyleAhmed Ashry; Aziza Hassan; Mahmoud Habiba; Ahmed El-Zayat; Mohamed El-Sharnouby; Hani Sewilam; Mahmoud Dawood. 2021. "The Impact of Dietary Curcumin on the Growth Performance, Intestinal Antibacterial Capacity, and Haemato-Biochemical Parameters of Gilthead Seabream (Sparus aurata)." Animals 11, no. 6: 1779.
The current investigation evaluated the alleviating effects of vitamin C and vitamin E on oxidative stress, hematobiochemical, and histopathological changes in the kidney induced by copper sulfate (CuSO4) toxicity in chickens. Two hundred and fifty-one-day-old male broiler chicks were randomly allotted into five experimental groups (five replicates/group, ten chicks/replicate): 1st group—basal diet with no additives (control group), 2nd group—basal diet complemented with CuSO4 (300 mg/kg diet), 3rd group—basal diet with CuSO4 (300 mg/kg diet) + vitamin C (250 mg/kg diet), 4th group—basal diet with CuSO4 (300 mg/kg diet) + vitamin E (250 mg/kg diet), and 5th group—basal diet with CuSO4 (300 mg/kg diet) + vitamin C (250 mg/kg diet) + vitamin E (250 mg/kg diet) for a 42 day feeding period. The results showed a significant reduction in red blood cells (RBCs), hemoglobin (Hb) concentration, and hematocrit values as well as total leukocyte counts (WBCs), lymphocyte, heterophil, and monocyte counts in the CuSO4-intoxicated birds (2.42 × 106/µL, 9.54 g/dL, 26.02%, 15.80 × 103/µL, 7.86 × 103/µL, 5.26 × 103/µL, and 1.18 × 103/µL, respectively, at the 6th week) compared to (2.79 × 106/µL, 10.98 g/dL, 28.46%, 21.07 × 103/µL, 10.84 × 103/µL, 7.12 × 103/µL, and 1.60 × 103/µL, respectively) in the control group. Moreover, CuSO4-intoxicated birds showed hypoglycemia with a rise in serum uric acid and creatinine levels (122.68, 5.18, and 0.78 mg/dL at the 6th week) compared to (159.46, 4.41, and 0.61 mg/dL) in the control group. The CuSO4 toxicity in birds induced oxidative stress, indicated by a high serum malondialdehyde level (MDA) and diminished activity of the antioxidant enzymes (glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD)) (2.01 nmol/mL, 37.66 U/mL, and 2.91 U/mL, respectively, at the 6th week) compared to (1.34 nmol/mL, 57.00 U/mL, 4.99 U/mL, respectively) in the control group. High doses of Cu exposure caused severe microscopic alterations in kidney architecture. The addition of vitamins C and E, singularly or in combination, displayed a beneficial effect in alleviating these harmful effects of Cu toxicity. These findings showed the possible mitigating impacts of dietary antioxidants on the hematobiochemical alterations, oxidative stress, and kidney damage induced by CuSO4 toxicity.
Mohamed Hashem; Sahar Abd El Hamied; Eman Ahmed; Shimaa Amer; Aziza Hassan. Alleviating Effects of Vitamins C and E Supplementation on Oxidative Stress, Hematobiochemical, and Histopathological Alterations Caused by Copper Toxicity in Broiler Chickens. Animals 2021, 11, 1739 .
AMA StyleMohamed Hashem, Sahar Abd El Hamied, Eman Ahmed, Shimaa Amer, Aziza Hassan. Alleviating Effects of Vitamins C and E Supplementation on Oxidative Stress, Hematobiochemical, and Histopathological Alterations Caused by Copper Toxicity in Broiler Chickens. Animals. 2021; 11 (6):1739.
Chicago/Turabian StyleMohamed Hashem; Sahar Abd El Hamied; Eman Ahmed; Shimaa Amer; Aziza Hassan. 2021. "Alleviating Effects of Vitamins C and E Supplementation on Oxidative Stress, Hematobiochemical, and Histopathological Alterations Caused by Copper Toxicity in Broiler Chickens." Animals 11, no. 6: 1739.
As the world population increases, there will be an increase in demand for poultry meat. To satisfy this requirement, sustainably will be a significant challenge as different countries in the world face different health problems. Additionally, for decades, poultry nutritionists have worked on sustainability in broiler manufacturing plants. The usage of short- and medium-chain fatty acids (MCFAs), which have positive effects as growth promoters and immunostimulants, is a pioneering way to decrease the cost of diets and improve environmentally friendly and sustainable production in this critical era. This study aimed at evaluating the influences of alpha-monoglyceride (alpha-monolaurin) on the growth performance, biochemical parameters, lipid profiles, liver oxidative stress, and immune response in broilers. Alpha-monolaurin was included at the levels of 0 (control), 0.25, 0.5, and 1 g/kg in broiler diets. After 33 days, dietary supplementation of alpha-monolaurin showed no impact on the feed intake; however, broilers fed 0.5 g/kg feed displayed a significantly reduced food conversion ratio, as compared to the control (p ≤ 0.05). However, body weight and body weight gain tended to increase after 0.25 g/kg alpha-monolaurin supplementation, as compared to other groups. The highest European production efficiency factor (EPEF) was obtained with 0.25 g/kg alpha-monolaurin supplementation. The ether extract utilization and spleen weight were improved substantially (p ≤ 0.05) by alpha-monolaurin supplementation, but muscle and other organ weights were not affected. Plasma alanine transaminase (ALT) was decreased significantly by alpha-monolaurin supplementation, while plasma total protein and albumin were enhanced (p ≤ 0.05). Interestingly, broilers fed alpha-monolaurin displayed an increased antibody titer of the Newcastle disease (ND) virus and muscle unsaturated fatty acid content, and a decreased liver content of malondialdehyde (MDA). It could be assumed that dietary supplementation of alpha-monolaurin positively impacts birds’ feed efficiency and immune response and can be applied as a natural immune enhancer in the broiler industry.
Ahmed Saleh; Bahaa El-Gharabawy; Aziza Hassan; Nemat Badawi; Yahya Eid; Shaimaa Selim; Mustafa Shukry; Mahmoud Dawood. Effect of Dietary Inclusion of Alpha-Monolaurin on the Growth Performance, Lipid Peroxidation, and Immunity Response in Broilers. Sustainability 2021, 13, 5231 .
AMA StyleAhmed Saleh, Bahaa El-Gharabawy, Aziza Hassan, Nemat Badawi, Yahya Eid, Shaimaa Selim, Mustafa Shukry, Mahmoud Dawood. Effect of Dietary Inclusion of Alpha-Monolaurin on the Growth Performance, Lipid Peroxidation, and Immunity Response in Broilers. Sustainability. 2021; 13 (9):5231.
Chicago/Turabian StyleAhmed Saleh; Bahaa El-Gharabawy; Aziza Hassan; Nemat Badawi; Yahya Eid; Shaimaa Selim; Mustafa Shukry; Mahmoud Dawood. 2021. "Effect of Dietary Inclusion of Alpha-Monolaurin on the Growth Performance, Lipid Peroxidation, and Immunity Response in Broilers." Sustainability 13, no. 9: 5231.
Objectives: Due to the expanded bacterial genetic tolerance to antibiotics through different mechanisms, infectious diseases of MDR bacteria are difficult for treatment. Consequently, we synthesized drug conjugated nanoparticles to dissolve this problem. Moreover, the present study aims to display the cell death status treated with cefotaxime-CS-AgNPs and also, apoptosis pathways of human RPE-1 normal cells and human MCF-7 breast cancer cells. Methods: Here, we demonstrate the possibility to synthesize AgNPs and conjugate them with cefotaxime to survey the probability of cefotaxime-CS-AgNPs as an antimicrobial agent against cefotaxime-resistant strains E. coli and MRSA. Results: TEM showed the size of AgNPs, CS-AgNPs and cefotaxime-CS-AgNPs ranged from 7.42 to 18.3 nm, 8.05– 23.89 nm and 8.48– 25.3 nm, respectively, with a spherical shape. The cefotaxime-CS-AgNPs enhanced the high antimicrobial properties compared to AgNPs or pure antibiotic. The MIC of Cefotaxime-CS-AgNPs ranged from 3 μg/mL to 8 μg/mL against tested E. coli and MRSA bacteria. Consequently, the highest reduction in the MIC of cefotaxime-CS-AgNPs was noted against tested strains ranging from 22% to 96%. Comparing cefotaime-CS-AgNPs to AgNPs we showed that cefotaime-CS-AgNPs have no cytotoxic effect on normal cells at even 12 μg/mL for 24 hrs. The IC50 for the AgNPs and cefotaxime-CS-AgNPs was 12 μg/mL for human RPE-1 normal cells and human MCF-7 breast cancer cell lines. The pro-apoptotic genes p53, p21, and Bax of cancer cell lines significantly upregulated followed by downregulated by anti-apoptotic gene Bcl-2 after 48 hrs at 24 μg/mL, and this concentration represents the most effective dose. Conclusion: Results enhanced the conjugating utility in old unresponsive cefotaxime to AgNPs to restore its efficiency against previous strains and demonstrated potential therapeutic applications of cefotaxime-CS-AgNPs. Moreover, this research gives remarkable insights for designing nanoscale delivery and curative systems that have a pronounced cytotoxic activity on cancer cells and are safe to normal cells.
Eman M Halawani; Aziza M Hassan; Sanaa Mf Gad El-Rab. Nanoformulation of Biogenic Cefotaxime-Conjugated-Silver Nanoparticles for Enhanced Antibacterial Efficacy Against Multidrug-Resistant Bacteria and Anticancer Studies. International Journal of Nanomedicine 2020, ume 15, 1889 -1901.
AMA StyleEman M Halawani, Aziza M Hassan, Sanaa Mf Gad El-Rab. Nanoformulation of Biogenic Cefotaxime-Conjugated-Silver Nanoparticles for Enhanced Antibacterial Efficacy Against Multidrug-Resistant Bacteria and Anticancer Studies. International Journal of Nanomedicine. 2020; ume 15 ():1889-1901.
Chicago/Turabian StyleEman M Halawani; Aziza M Hassan; Sanaa Mf Gad El-Rab. 2020. "Nanoformulation of Biogenic Cefotaxime-Conjugated-Silver Nanoparticles for Enhanced Antibacterial Efficacy Against Multidrug-Resistant Bacteria and Anticancer Studies." International Journal of Nanomedicine ume 15, no. : 1889-1901.
The aims of this study were to determine the polyphone content of grape seed extract (GSE) and to assess their protective effects against zearalenone (ZEN)-induced maternal toxicity and in utero development defects in mice. Five groups of pregnant mice were treated orally during days 6–13 of gestation as follows: control group, corn oil as vehicle (0.1 ml/mice)-treated group, ZEN-treated group (25 mg/kg b.w), GSE-treated group (150 mg/kg b.w.), and ZEN plus GSE-treated group. All animals were sacrificed on the 19th day of gestation and samples of bone marrow were collected for the micronucleus assay. The maternal and developmental toxicity were carried out. The HPLC analyses revealed that GES is rich in gallic acid, syringic acid, vanillin, quercetin, and coumaric acid. ZEN administration resulted in severe maternal and developmental toxicity which included an increase of micronuclei formation in bone marrow, decreased maternal weight gain, and litter weight. It also induces fetal growth retardation, increased number of the aborted dams and resorbed fetuses, abnormality of fetal bone ossification, and number of fetuses with a hematoma. GSE showed positive effects on the pregnant mice and the developing fetuses. Moreover, it counteracted the detrimental effects of ZEN in dams and fetuses. It could be concluded that polyphenols in GSE are a promising candidate to protect against ZEN toxicity in highly endemic areas.
Nouf J. Althali; Aziza M. Hassan; Mosaad A. Abdel-Wahhab. Effect of grape seed extract on maternal toxicity and in utero development in mice treated with zearalenone. Environmental Science and Pollution Research 2019, 26, 5990 -5999.
AMA StyleNouf J. Althali, Aziza M. Hassan, Mosaad A. Abdel-Wahhab. Effect of grape seed extract on maternal toxicity and in utero development in mice treated with zearalenone. Environmental Science and Pollution Research. 2019; 26 (6):5990-5999.
Chicago/Turabian StyleNouf J. Althali; Aziza M. Hassan; Mosaad A. Abdel-Wahhab. 2019. "Effect of grape seed extract on maternal toxicity and in utero development in mice treated with zearalenone." Environmental Science and Pollution Research 26, no. 6: 5990-5999.
Gold nanoparticles (AuNP) and their conjugates have been gaining a great deal of recognition in the medical field. Meanwhile, extended-spectrum β-lactamases (ESBL)-producing bacteria are also demonstrating a challenging problem for health care. The aim of this study was the biosynthesis of AuNP using Rosa damascenes petal extract and conjugation of ceftriaxone antibiotic (Cef-AuNP) in inhibiting ESBL-producing bacteria and study of in vitro anticancer activity. Characterization of the synthesized AuNP and Cef-AuNP was studied. ESB-Lproducing strains, Acinetobacter baumannii ACI1 and Pseudomonas aeruginosa PSE4 were used for testing the efficacy of Cef-AuNP. The cells of MCF-7 breast cancer were treated with previous AuNP and Cef-AuNP at different time intervals. Cytotoxicity effects of apoptosis and its molecular mechanism were evaluated. Ultraviolet-visible spectroscopy and Fourier transform infrared spectroscopy established the formation of AuNP and Cef-AuNP. Transmission electron microscope demonstrated that the formed nanoparticles were of different shapes with sizes of 15~35 nm and conjugation was established by a slight increase in size. Minimum inhibitory concentration (MIC) values of Cef-AuNP against tested strains were obtained as 3.6 and 4 μg/ml, respectively. Cef-AuNP demonstrated a decrease in the MIC of ceftriaxone down to more than 27 folds on the studied strains. The biosynthesized AuNP displayed apoptotic and time-dependent cytotoxic effects in the cells of MCF-7 at a concentration of 0.1 μg/ml medium. The Cef-AuNP have low significant effects on MCF-7 cells. These results enhance the conjugating utility in old unresponsive ceftriaxone with AuNP to restore its efficiency against otherwise resistant bacterial pathogens. Additionally, AuNP may be used as an alternative chemotherapeutic treatment of MCF-7 cancer cells.
Sanaa M.F. Gad El-Rab; Eman M. Halawani; Aziza M. Hassan. Formulation of Ceftriaxone Conjugated Gold Nanoparticles and Their Medical Applications against Extended-Spectrum β -Lactamase Producing Bacteria and Breast Cancer. Journal of Microbiology and Biotechnology 2018, 28, 1563 -1572.
AMA StyleSanaa M.F. Gad El-Rab, Eman M. Halawani, Aziza M. Hassan. Formulation of Ceftriaxone Conjugated Gold Nanoparticles and Their Medical Applications against Extended-Spectrum β -Lactamase Producing Bacteria and Breast Cancer. Journal of Microbiology and Biotechnology. 2018; 28 (9):1563-1572.
Chicago/Turabian StyleSanaa M.F. Gad El-Rab; Eman M. Halawani; Aziza M. Hassan. 2018. "Formulation of Ceftriaxone Conjugated Gold Nanoparticles and Their Medical Applications against Extended-Spectrum β -Lactamase Producing Bacteria and Breast Cancer." Journal of Microbiology and Biotechnology 28, no. 9: 1563-1572.
This study aimed to assess the disturbances in intestinal genes expression and DNA fragmentation in mice treated orally with aflatoxin B1 (AFB1) or aflatoxin M1 (AFM1) and the protective activity of Lactobacillus plantarum (LP). Male Balb/c mice were divided into 6 groups including the control group, the group treated with 2 mg/kg b.w of LP (2 × 109 cfu/mL), the groups treated with AFB1 or AFM1 (100 μg/kg b.w), and the groups treated with AFB1 or AFM1 during, after or before LP. Small intestines were collected for the determination of DNA fragmentation, gene expression and target protein content. The results showed that AFB1 or AFM1 increased DNA fragmentation, down regulated the expressions of caspase-3, caspase-9, CYP3A13, Bax and p53 as well as up-regulated the expression of TNF-α and Bcl-2 and their target proteins. LP succeeded to alleviate the disturbances in DNA fragmentation and the expression of these genes. The improvement was more pronounced in the group co-administered with the toxins plus LP. It could be concluded that AFB1 and AFM1 induced disturbances in intestinal function via the disturbances in DNA fragmentation and genes expression. LP induced a potential protective effect and is considered a promising agent against the genotoxicity induced by these mycotoxins.
Rania Jebali; Jalila Ben Salah-Abbès; Samir Abbès; Aziza Hassan; Sekena H. Abdel-Aziem; Aziza A. El-Nekeety; Ridha Oueslati; Mosaad A. Abdel-Wahhab. Lactobacillus plantarum alleviate aflatoxins (B 1 and M 1 ) induced disturbances in the intestinal genes expression and DNA fragmentation in mice. Toxicon 2018, 146, 13 -23.
AMA StyleRania Jebali, Jalila Ben Salah-Abbès, Samir Abbès, Aziza Hassan, Sekena H. Abdel-Aziem, Aziza A. El-Nekeety, Ridha Oueslati, Mosaad A. Abdel-Wahhab. Lactobacillus plantarum alleviate aflatoxins (B 1 and M 1 ) induced disturbances in the intestinal genes expression and DNA fragmentation in mice. Toxicon. 2018; 146 ():13-23.
Chicago/Turabian StyleRania Jebali; Jalila Ben Salah-Abbès; Samir Abbès; Aziza Hassan; Sekena H. Abdel-Aziem; Aziza A. El-Nekeety; Ridha Oueslati; Mosaad A. Abdel-Wahhab. 2018. "Lactobacillus plantarum alleviate aflatoxins (B 1 and M 1 ) induced disturbances in the intestinal genes expression and DNA fragmentation in mice." Toxicon 146, no. : 13-23.
Cadmium (Cd) is an important inorganic toxicant widely distributed in the environment because of its various industrial uses. The aims of the current study were to investigate the efficacy of purified Egyptian and Tunisian montmorillonite clays (EMC and TMC) to inhibit genotoxicity and histological alterations induced by cadmium chloride (CdCl2) utilizing the Nile tilapia fish as an in vivo model. Chromosomal aberrations (CAs), micronucleus (MN) frequencies and DNA fingerprinting profile were genotoxic end points and histopathological changes that were used in this investigation. Six groups of fish were treated for 2 weeks and included control group, CdCl2-treated group and groups treated with EMC or TMC alone or in combination with CdCl2. The present results revealed that, treatment of fish with CdCl2 exhibited significant increased in the number of micronucleated erythrocytes (MnRBCs), frequency of CAs and instability of genomic DNA. Treatment of EMC and TMC in combination with CdCl2 significantly reduced the frequency of MnRBCs by the percentage of 53.28% and 60.77% and the frequency of CAs by 43.91% and 52.17% respectively. As well as, normalized DNA fingerprinting profile and significantly improved histopathological picture induced by Cadmium treatment. It is worth mention that both clays have the ability to tightly bind CdCl2 and decreased its cytotoxicity and genotoxicity; however, Tunisian clay was more efficient in binding with the CdCl2 than Egyptian clay.
Karima F. Mahrous; Aziza Hassan; Hasnaa Radwan; A M Mahmoud. Inhibition of cadmium- induced genotoxicity and histopathological changes in Nile tilapia fish by Egyptian and Tunisian montmorillonite clay. Ecotoxicology and Environmental Safety 2015, 119, 140 -147.
AMA StyleKarima F. Mahrous, Aziza Hassan, Hasnaa Radwan, A M Mahmoud. Inhibition of cadmium- induced genotoxicity and histopathological changes in Nile tilapia fish by Egyptian and Tunisian montmorillonite clay. Ecotoxicology and Environmental Safety. 2015; 119 ():140-147.
Chicago/Turabian StyleKarima F. Mahrous; Aziza Hassan; Hasnaa Radwan; A M Mahmoud. 2015. "Inhibition of cadmium- induced genotoxicity and histopathological changes in Nile tilapia fish by Egyptian and Tunisian montmorillonite clay." Ecotoxicology and Environmental Safety 119, no. : 140-147.
This study was conducted to prepare and characterize activated carbon (AC) and to evaluate its protective effect against deoxynivalenol (DON) toxicity in rats compared to Egyptian montmorillonite (EM). AC was prepared using a single-step chemical activation with phosphoric acid (H3PO4). The resulted AC has a high surface area and a high total pore volume. Male Sprague-Dawley rats were divided into 6 groups (n = 10) and treated for 3 weeks as follow: the control group, the groups fed AC or EM-supplemented diet (0.5% w/w), the group treated orally with DON (5 mg/kg b.w.) and the groups fed AC or EM-supplemented diet and treated with DON. Blood and liver samples were collected for different analyses. Treatment with DON increased liver function enzymes, lipid peroxidation, tumor necrosis factor α, DNA fragmentation, decreased hepatic glutathione content, up regulating mRNA Fas and TNF-α genes expression and increased micronucleated polychromatic erythrocytes and normochromatic erythrocytes in bone marrow. Co-treatment of DON plus AC or EM succeeded to normalize the levels of the biochemical parameters, reduced the cytotoxicity of bone marrow and ameliorated the hepatic genotoxicity. Moreover, AC was more effective than EM and has a high affinity to adsorb DON and to reduce its cytotoxicity and genotoxicity.
Dr. Mosaad Attia Abdel-Wahhab; Ahmed El-Kady; Aziza Hassan; Omaima M. Abd El-Moneim; Sekena Abdel Aziem. Effectiveness of activated carbon and Egyptian montmorillonite in the protection against deoxynivalenol-induced cytotoxicity and genotoxicity in rats. Food and Chemical Toxicology 2015, 83, 174 -182.
AMA StyleDr. Mosaad Attia Abdel-Wahhab, Ahmed El-Kady, Aziza Hassan, Omaima M. Abd El-Moneim, Sekena Abdel Aziem. Effectiveness of activated carbon and Egyptian montmorillonite in the protection against deoxynivalenol-induced cytotoxicity and genotoxicity in rats. Food and Chemical Toxicology. 2015; 83 ():174-182.
Chicago/Turabian StyleDr. Mosaad Attia Abdel-Wahhab; Ahmed El-Kady; Aziza Hassan; Omaima M. Abd El-Moneim; Sekena Abdel Aziem. 2015. "Effectiveness of activated carbon and Egyptian montmorillonite in the protection against deoxynivalenol-induced cytotoxicity and genotoxicity in rats." Food and Chemical Toxicology 83, no. : 174-182.
This study was conducted to evaluate the protective role of quercetin (Q) against the cytotoxicity, DNA damage and oxidative stress in rats fed aflatoxin (AFs)-contaminated diet. Female Sprague–Dawley rats were divided into six groups and treated for 21 days as follows: the control group; the group fed AFs-contaminated diet (1.4 mg/kg diet); the groups treated orally with Q at low or high dose (50 and 100 mg/kg b.w.) and the groups AFs-contaminated diet plus low or high dose of Q. At the end of experiment, blood and liver samples were collected for biochemical, histological, histochemical and genetic analyses. The results indicated that animal fed AFs-contaminated diet showed significant increase in serum biochemical parameters, oxidative stress markers and DNA fragmentation accompanied with significant decrease in total proteins, GPX, SOD, DNA and RNA content and fatty acid synthase (Fas) and TNFα gene expression in the liver tissue. Q at the two tested doses succeeded to normalize the biochemical parameters, improved the content of nucleic acids in hepatic tissues, the gene expression, the histopathological and histochemical picture of the liver. It could be concluded that Q has a potential antioxidant activity, a protective action and regulated the alteration of genes expression induced by AFs.
Aziza El-Nekeety; Sekena H. Abdel-Azeim; Aziza Hassan; Nabila S. Hassan; Nabila Aly; Dr. Mosaad Attia Abdel-Wahhab. Quercetin inhibits the cytotoxicity and oxidative stress in liver of rats fed aflatoxin-contaminated diet. Toxicology Reports 2014, 1, 319 -329.
AMA StyleAziza El-Nekeety, Sekena H. Abdel-Azeim, Aziza Hassan, Nabila S. Hassan, Nabila Aly, Dr. Mosaad Attia Abdel-Wahhab. Quercetin inhibits the cytotoxicity and oxidative stress in liver of rats fed aflatoxin-contaminated diet. Toxicology Reports. 2014; 1 ():319-329.
Chicago/Turabian StyleAziza El-Nekeety; Sekena H. Abdel-Azeim; Aziza Hassan; Nabila S. Hassan; Nabila Aly; Dr. Mosaad Attia Abdel-Wahhab. 2014. "Quercetin inhibits the cytotoxicity and oxidative stress in liver of rats fed aflatoxin-contaminated diet." Toxicology Reports 1, no. : 319-329.
Aflatoxins and fumonisins are important food-borne mycotoxins implicated in human health and have cytotoxic effects. The aims of the current study were to evaluate the protective role of Panax ginseng extract (PGE) against the synergistic effect of subchronic administration of aflatoxin B1 (AFB1) and fumonisin B1 (FB1) on DNA and gene expression in rat. Female Sprague–Dawley rats were divided into eight groups (ten rats/group) and treated for 12 weeks including the control group, the group having received AFB1 (80 µg/kg bw), the group having received FB1 (100 µg/kg bw), the group having received AFB1 plus FB1 and the groups having received PGE (20 mg/kg bw) alone or with AFB1 and/or FB1. At the end of experiment, liver and kidney were collected for the determination of DNA fragmentation, lipid peroxidation (LP), glutathione (GSH) contents and alterations in gene expression. The results indicated that these mycotoxins increased DNA fragmentation, LP and decreased GSH content in liver and kidney and down-regulated gene expression of antioxidants enzymes. The combined treatments with AFB1 and/or FB1 plus PGE suppressed DNA fragmentation only in the liver, normalized LP and increased GSH in the liver and kidney as well as up-regulated the expression of GPx, SOD1 and CAT mRNA. It could be concluded that AFB1 and FB1 have synergistic genotoxic effects. PGE induced protective effects against their oxidative stress and genotoxicity through its antioxidant properties.
Aziza Hassan; Sekena Abdel Aziem; Aziza El-Nekeety; Dr. Mosaad Attia Abdel-Wahhab. Panax ginseng extract modulates oxidative stress, DNA fragmentation and up-regulate gene expression in rats sub chronically treated with aflatoxin B1 and fumonisin B1. Cytotechnology 2014, 67, 861 -871.
AMA StyleAziza Hassan, Sekena Abdel Aziem, Aziza El-Nekeety, Dr. Mosaad Attia Abdel-Wahhab. Panax ginseng extract modulates oxidative stress, DNA fragmentation and up-regulate gene expression in rats sub chronically treated with aflatoxin B1 and fumonisin B1. Cytotechnology. 2014; 67 (5):861-871.
Chicago/Turabian StyleAziza Hassan; Sekena Abdel Aziem; Aziza El-Nekeety; Dr. Mosaad Attia Abdel-Wahhab. 2014. "Panax ginseng extract modulates oxidative stress, DNA fragmentation and up-regulate gene expression in rats sub chronically treated with aflatoxin B1 and fumonisin B1." Cytotechnology 67, no. 5: 861-871.
The aims of the current work were to evaluate the hepatoprotective effect of calendula flowers and/or thyme leave extracts on aflatoxins (AFs)-induced oxidative stress, genotoxicity and alteration of p53 bax and bcl2 gene expressions. Eighty male Sprague–Dawley rats were divided into eight equal groups including: the control group, the group fed AFs-contaminated diet (2.5 mg/kg diet) for 5 weeks, the groups treated orally with thyme and/or calendula extract (0.5 g/kg b.w) for 6 weeks and the groups pretreated orally with thyme and/or calendula extract 1 week before and during AFs treatment for further 5 weeks. Blood, liver and bone marrow samples were collected for biochemical analysis, gene expression, DNA fragmentation and micronucleus assay. The results showed that AFs induced significant alterations in oxidative stress markers, increased serum AFP and inflammatory cytokine, percentage of DNA fragmentation, the expression of pro-apoptotic gene p53 and bax accompanied with a decrease in the expression of bcl2. Animals treated with the extracts 1 week before AFs treatment showed a significant decrease in oxidative damage markers, micronucleated cells, DNA fragmentation and modulation of the expression of pro-apoptotic genes. These results suggested that both calendula and thyme extracts had anti-genotoxic effects due to their higher content of total phenolic compounds.
Sekena Abdel Aziem; Aziza Hassan; Ezzeldein S. El-Denshary; Mohamed Hamzawy; Fathia Mannaa; Dr. Mosaad Attia Abdel-Wahhab. Ameliorative effects of thyme and calendula extracts alone or in combination against aflatoxins-induced oxidative stress and genotoxicity in rat liver. Cytotechnology 2013, 66, 457 -470.
AMA StyleSekena Abdel Aziem, Aziza Hassan, Ezzeldein S. El-Denshary, Mohamed Hamzawy, Fathia Mannaa, Dr. Mosaad Attia Abdel-Wahhab. Ameliorative effects of thyme and calendula extracts alone or in combination against aflatoxins-induced oxidative stress and genotoxicity in rat liver. Cytotechnology. 2013; 66 (3):457-470.
Chicago/Turabian StyleSekena Abdel Aziem; Aziza Hassan; Ezzeldein S. El-Denshary; Mohamed Hamzawy; Fathia Mannaa; Dr. Mosaad Attia Abdel-Wahhab. 2013. "Ameliorative effects of thyme and calendula extracts alone or in combination against aflatoxins-induced oxidative stress and genotoxicity in rat liver." Cytotechnology 66, no. 3: 457-470.
Spirulina (SPN) and Whey protein (WPC) are being touted as functional foods with a number of health benefits. SPN is blue green algae while WPC is a protein complex derived from milk and both have strong antioxidant activity and provoke a free radical scavenging enzyme system. The aim of the present study was to evaluate the antioxidant potentials of SPN and WPC to regulate the alteration of genes' expression and counteract oxidative stress in rats during aflatoxecosis. Eighty male Sprague–Dawley rats were divided into eight groups, which included the control group, the group fed with aflatoxins (AFs)-contaminated diet (2.5 mg/kg diet) for 30 day, the group treated orally with WPC (300 mg/kg b.w.), the group treated orally with SPN (50 mg/kg b.w), the group treated orally with WPC plus SPN and the groups fed with AFs-contaminated diet and treated orally with WPC, SPN and/or WPC. Oxidative stress markers and gene expression were assayed in liver and testis and the damage of DNA was evaluated by DNA fragmentation and micronucleus tests. The results demonstrated that supplementation of SPN and/or WPC reduced the oxidative stress induced by AFs as indicated by decreased lipid peroxidation level, increased glutathione content and up-regulated PHGPx gene expression. Both agents succeed to inhibit DNA damage as indicated by the down-regulation of Fas gene expression, and decreased the percentage of DNA fragmentation and micronucleated erythrocytes. Moreover, WPC was found to be effective than SPN and the combined treatment was more effective than the single treatment. It could be concluded that both SPN and WPC induced a protective action and regulated the alteration of genes expression induced by AFs; however, the combined treatment may be useful than the single treatment.
Aziza Hassan; Sekena Abdel Aziem; Dr. Mosaad Attia Abdel-Wahhab. Modulation of DNA damage and alteration of gene expression during aflatoxicosis via dietary supplementation of Spirulina (Arthrospira) and whey protein concentrate. Ecotoxicology and Environmental Safety 2012, 79, 294 -300.
AMA StyleAziza Hassan, Sekena Abdel Aziem, Dr. Mosaad Attia Abdel-Wahhab. Modulation of DNA damage and alteration of gene expression during aflatoxicosis via dietary supplementation of Spirulina (Arthrospira) and whey protein concentrate. Ecotoxicology and Environmental Safety. 2012; 79 ():294-300.
Chicago/Turabian StyleAziza Hassan; Sekena Abdel Aziem; Dr. Mosaad Attia Abdel-Wahhab. 2012. "Modulation of DNA damage and alteration of gene expression during aflatoxicosis via dietary supplementation of Spirulina (Arthrospira) and whey protein concentrate." Ecotoxicology and Environmental Safety 79, no. : 294-300.
Exposure to chemicals like benzo(a)pyrene (BaP) can lead to structural changes in DNA and as a consequence to increased incidence of diseases with a genetic basis, as well as oxidative stress in the testis. However its ability to induce oxidative DNA damage in germ cells is not fully investigated. In the present study, BaP was used to induce 8-hydroxydeoxyguanosine (8-OHdG), a specific DNA adducts for oxidative DNA damage, in testis and epididymal sperm and the possible protection role of radish and/or cress was investigated. The results revealed that BaP induced a significant increase in DNA damage in both tissues, as indicated by increased DNA strand breaks in a fluorimetric analysis of DNA unwinding (FADU). Furthermore, it increased the oxidative damage in epididymal sperm, as indicated by the increase in sperm abnormalities, lipid peroxidation (LPO), accompanied with a decrease in glutathione content (GSH), sperm count and sperm motility as well as induction of filtration in the histology of the testis. Treatment with radish and/or cress oil prior to BaP injection succeeded in reducing the germ cell genotoxicity as indicated by the decrease in DNA damage, 8-OHdG levels, sperm abnormalities, LPO level and increased sperm counts, motility and GSH content. Moreover, cress was found to be effective than radish and the combined treatment was more effective than the single treatment. It could be concluded that, pretreatment with radish and/or cress improved the epididymal sperm quality and reduced the genotoxicity and DNA damage induced by BaP, thereby declaring the protective role of radish and cress.
Aziza Hassan; Sally S Alam; Sekena Abdel Aziem; Kawkab A. Ahmed. Benzo-a-pyrene induced genotoxicity and cytotoxicity in germ cells of mice: Intervention of radish and cress. Journal of Genetic Engineering and Biotechnology 2011, 9, 65 -72.
AMA StyleAziza Hassan, Sally S Alam, Sekena Abdel Aziem, Kawkab A. Ahmed. Benzo-a-pyrene induced genotoxicity and cytotoxicity in germ cells of mice: Intervention of radish and cress. Journal of Genetic Engineering and Biotechnology. 2011; 9 (1):65-72.
Chicago/Turabian StyleAziza Hassan; Sally S Alam; Sekena Abdel Aziem; Kawkab A. Ahmed. 2011. "Benzo-a-pyrene induced genotoxicity and cytotoxicity in germ cells of mice: Intervention of radish and cress." Journal of Genetic Engineering and Biotechnology 9, no. 1: 65-72.
Aflatoxins (AF) are among the most potent naturally occurring carcinogens and aflatoxin-B1 (AFB1) is classified as a group-1 carcinogen. Since the ingestion of aflatoxins-contaminated food is associated with several liver diseases, the aim of the present study was to evaluate whether AF-induced damage in rats can be counteracted by feeding with whey-protein concentrates (WPC) and Korean ginseng extract (KGE). Eighty male Sprague-Dawley rats were divided into eight equal groups and treated daily for 30 days as follows: a control group (fed an AF-free diet), a group fed ad libitum an AF-contaminated diet (2.5 mg/kg diet), a group treated orally with WPC (0.5 ml/rat/day), a group treated orally with KGE (20 mg/kg body weight), a group treated orally with WPC + KGE, and three groups that were fed the AF-contaminated diet and were treated orally with WPC, KGE or WPC + KGE, respectively. Throughout the experimental period, animals received WPC or KGE during the consumption of their respective diet. Bone-marrow micronucleus formation, DNA fragmentation, fatty-acid synthesis (FAS) and phospholipid-hydroperoxide-glutathione-peroxidase (PHGPx) mRNA expression, and oxidative stress were assayed in liver and testis. The results indicated that ingestion of aflatoxin resulted in a significant increase in micronucleated normochromatic erythrocytes (Mn-NCE) in bone marrow, DNA fragmentation, FAS mRNA expression and lipid peroxidation in both organs, and a significant decrease in micronucleated polychromatic erythrocytes/micronucleated normochromatic erythrocytes (PCE/NCE) ratio in bone marrow, PHGPx gene expression and GSH in liver and testis. Treatments with WPC and/or KGE had a significant effect on Mn-NCE or the PCE/NCE ratio in bone marrow. However, KGE or KGE + WPC increased PHGPx gene expression and GSH in testis accompanied with a significant decrease in lipid peroxidation in liver and testis and FAS-mRNA expression in liver. WPC, KGE or WPC + KGE treatments combined with exposure to an AF-contaminated diet restored all the test parameters towards control values, although they did not fully reverse the effects of the aflatoxins. It is suggested that the genotoxicity of aflatoxins can be in part prevented by dietary supplementation with WPC, KGE or their combination.
Sekena Abdel Aziem; Aziza Hassan; Dr. Mosaad Attia Abdel-Wahhab. Dietary supplementation with whey protein and ginseng extract counteracts oxidative stress and DNA damage in rats fed an aflatoxin-contaminated diet. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 2011, 723, 65 -71.
AMA StyleSekena Abdel Aziem, Aziza Hassan, Dr. Mosaad Attia Abdel-Wahhab. Dietary supplementation with whey protein and ginseng extract counteracts oxidative stress and DNA damage in rats fed an aflatoxin-contaminated diet. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2011; 723 (1):65-71.
Chicago/Turabian StyleSekena Abdel Aziem; Aziza Hassan; Dr. Mosaad Attia Abdel-Wahhab. 2011. "Dietary supplementation with whey protein and ginseng extract counteracts oxidative stress and DNA damage in rats fed an aflatoxin-contaminated diet." Mutation Research/Genetic Toxicology and Environmental Mutagenesis 723, no. 1: 65-71.
The current study was conducted to evaluate the ability of Egyptian bentonite (EB) and montmorillonite (EM) for the prevention of genotoxicity, histochemical and biochemical changes induced by aflatoxin B(1) (AFB(1)) using the micronucleus (MN) assay, chromosomal aberrations and DNA fragmentation analysis in Tilapia fish. Six groups of fish were treated for 3 weeks and included the control group, AFB(1)-treated group and the groups treated with EB or EM alone or in combination with AFB(1). At the end of experiment period, blood samples were collected for MN, testosterone and biochemical assays. Chromosomal aberrations were determined in kidney tissues, DNA fragmentation test was determined in liver and testis, whereas histochemical study was carried out on liver, testis and gills. The results indicated that a significant decrease in total protein, albumin, globulin, testosterone and DNA content in liver, gills and testis accompanied with a significant increase in number of micronucleated erythrocytes (MnRBCs), total chromosomal aberrations in kidney and DNA fragmentation in testis and liver of fish received AFB(1) alone. Fish treated with EB or EM alone were comparable to the control regarding the biochemical parameters except testosterone in EB-treated group which was significantly decreased. Both clays did not induce any significant differences in number of MnRBCs, chromosomal aberrations in the kidney, DNA fragmentation in testis, but not in liver of EB-treated group. The combined treatment with AFB(1) and EB or EM succeeded to improve all the tested parameters towards the control values although it did not normalize them. Moreover, the improvement was pronounced in the group received EM plus AFB(1). It could be concluded that EB and EM have the ability to tightly bind AFB(1) in the gastrointestinal tract of fish resulting in decreasing its bioavailability. Moreover, the two tested clays were safe and can be used as potential aflatoxin binders in animal feed.
Aziza M. Hassan; Amany M. Kenawy; Wafaa T. Abbas; Mossad A. Abdel-Wahhab; Dr. Mosaad Attia Abdel-Wahhab. Prevention of cytogenetic, histochemical and biochemical alterations in Oreochromis niloticus by dietary supplement of sorbent materials. Ecotoxicology and Environmental Safety 2010, 73, 1890 -1895.
AMA StyleAziza M. Hassan, Amany M. Kenawy, Wafaa T. Abbas, Mossad A. Abdel-Wahhab, Dr. Mosaad Attia Abdel-Wahhab. Prevention of cytogenetic, histochemical and biochemical alterations in Oreochromis niloticus by dietary supplement of sorbent materials. Ecotoxicology and Environmental Safety. 2010; 73 (8):1890-1895.
Chicago/Turabian StyleAziza M. Hassan; Amany M. Kenawy; Wafaa T. Abbas; Mossad A. Abdel-Wahhab; Dr. Mosaad Attia Abdel-Wahhab. 2010. "Prevention of cytogenetic, histochemical and biochemical alterations in Oreochromis niloticus by dietary supplement of sorbent materials." Ecotoxicology and Environmental Safety 73, no. 8: 1890-1895.
Exposure to fumonisins (FB) is known to have toxic and carcinogenic effects in different animal species, and to express toxicity in cells via the induction of oxidative stress. The aim of the current study was to evaluate the protective effects of the ethanol extract of Aquilegia vulgaris L. against the oxidative stress and the genotoxicity using micronucleus assay and random amplified polymorphism DNA (RAPD-PCR) in FB-treated rats. Sixty mature female Sprague-Dawley were divided into six treatment groups and treated for 4 weeks as follow: the control group, the group fed FB-contaminated diet (200 mg/kg diet), the groups treated orally with the extract (5 and 10 mg/kg bw) and the groups fed FB-contaminated diet and treated with the extract at the two doses. The results showed that treatment with FB alone disturbed lipid profile in serum, increases Sa/So ratio, induces micronucleated polychromatic erythrocytes (Mn-PCEs) in bone marrow, increases DNA and RNA in liver accompanied with significant changes in histological picture The extract alone at the two tested doses did not induce any significant changes in the biochemical or histological picture. The combined treatment showed significant improvements in all biochemical, cytogenetic parameters tested and histological pictures in the liver tissues. Moreover, this improvement was more pronounced in the group received the high dose of the extract. It could be concluded that the ethanol extract of A. vulgaris induced its protective effect via the increase in the antioxidant capacity, inhibition of lipid peroxidation and scavenging of free radicals.
Aziza M. Hassan; Sherif R. Mohamed; Aziza A. El-Nekeety; Nabila S. Hassan; Mosaad A. Abdel-Wahhab. Aquilegia vulgaris L. extract counteracts oxidative stress and cytotoxicity of fumonisin in rats. Toxicon 2010, 56, 8 -18.
AMA StyleAziza M. Hassan, Sherif R. Mohamed, Aziza A. El-Nekeety, Nabila S. Hassan, Mosaad A. Abdel-Wahhab. Aquilegia vulgaris L. extract counteracts oxidative stress and cytotoxicity of fumonisin in rats. Toxicon. 2010; 56 (1):8-18.
Chicago/Turabian StyleAziza M. Hassan; Sherif R. Mohamed; Aziza A. El-Nekeety; Nabila S. Hassan; Mosaad A. Abdel-Wahhab. 2010. "Aquilegia vulgaris L. extract counteracts oxidative stress and cytotoxicity of fumonisin in rats." Toxicon 56, no. 1: 8-18.