The generation of energy from renewable sources is a fundamental aspect for the sustainable development of society, and several energy sources such as solar, biomass, biogas, and wind must be used to the maximum to meet existing needs. In Chile, there are villages that are off-grid. A real case study is presented in this research. To meet the needs of this village we have proposed a mathematical optimization model using a CPLEX optimizer to generate the necessary energy power while minimizing the cost of energy (COE). In this study, different scenarios have been evaluated with respect to the existing energy availabilities, for example, in different periods of the year, demonstrated in terms of economic costs, the viability of resources such as biomass and biogas, and the viability of the energy production of wind power given the associated high costs. Finally, the effect of the use of renewable energy in consideration of CO₂ emissions is studied in our research.

The enzymatic hydrolysis of fish by-product proteins is traditionally carried out by mixing ground by-products with water. In addition, pH control is used to avoid pH drops. Higher costs are involved due to the use of pH control systems and the consequent energy cost in the drying stage. This work aimed to evaluate the effect of these conditions on the hydrolysis of salmon frame (SF) proteins, including the SF hydrolysis without added water. SF hydrolysis by subtilisin at 50, 75, and 100% SF under different pH regimes were evaluated by released α-amino (α-NH) groups, total nitrogen, degree of hydrolysis, and estimated peptide chain length (PCL) at 55 °C. The concentration of released α-NH groups was higher in the conditions with less added water. However, the nitrogen recovery decreased from 50 to 24% at 50 and 100% SF, respectively. Changing the SF/water ratio had a more significant effect than changing the pH regime. Estimated PCL changed from 5–7 to 7–9 at 50 and 100% SF, respectively. The operating conditions affected the hydrolysis performance and the molecular characteristics of the hydrolysate.