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A diet rich in nutrients should be implemented in order to boost the immune system and prevent infections. To investigate which nutrients are commonly consumed, an anonymous survey was given to 120 individuals and their responses were collected. The respondents answered questions relating to their health status, and their consumption of nutrients and supplements that produce immunomodulating effects. The participants were also asked about any prior viral, bacterial or fungal infections experienced, and in particular, infection frequency, course, and duration. The data collected were subjected to a statistical analyses to assess the relationship between the reported frequency of infections and nutrients consumed including vitamins D3, A, C, E, selenium, zinc, iron, β-carotene, omega-3 fatty acids as well as live active probiotic bacteria. The findings show that vitamin and mineral supplementation did not positively affect the duration, frequency, or course of infections in the surveyed sample. An exception was vitamin D3 supplementation that was correlated to sporadic incidence of viral infections. Conversely, immunity was positively affected by consumption of natural nutrients contained in whole food (vitamin C, iron, selenium, omega-3 fatty acids), evidenced by lower incidences and milder courses of infection.
Ewelina Polak; Agnieszka Stępień; Olga Gol; Jacek Tabarkiewicz. Potential Immunomodulatory Effects from Consumption of Nutrients in Whole Foods and Supplements on the Frequency and Course of Infection: Preliminary Results. Nutrients 2021, 13, 1157 .
AMA StyleEwelina Polak, Agnieszka Stępień, Olga Gol, Jacek Tabarkiewicz. Potential Immunomodulatory Effects from Consumption of Nutrients in Whole Foods and Supplements on the Frequency and Course of Infection: Preliminary Results. Nutrients. 2021; 13 (4):1157.
Chicago/Turabian StyleEwelina Polak; Agnieszka Stępień; Olga Gol; Jacek Tabarkiewicz. 2021. "Potential Immunomodulatory Effects from Consumption of Nutrients in Whole Foods and Supplements on the Frequency and Course of Infection: Preliminary Results." Nutrients 13, no. 4: 1157.
The purpose of the conducted study was to determine the effect of the applied organic and mineral fertilizer on the yield and the selected quality parameters of the obtained dried mass of Origanum vulgare L. A pot experiment was carried out involving two varieties of oregano (Aureum and Hot & Spicy), which were fertilized using organic and mineral fertilizer. The produced raw material underwent a drying process using the CPD-VMFD combination method, which involved convective pre-drying (40 °C and 0.8 m s−1) and vacuum-microwave finish drying at 240 W magnetron power. The resulting dry products were assessed in terms of color in CIE L * a * b * (Colour Ques spectrophotometer) and spectrophotometric analyses in order to determine the total content of polyphenols and antioxidant properties (ABTS and DPPH). Headspace solid phase micro-extraction (HS-SPME) from the obtained products was carried out, and their composition was determined by gas chromatography (GC-MS). The organic fertilizers used have resulted in the production of dry oregano herbs with a much higher bioactive potential than that of the herbs treated with material fertilizer. This phenomenon could be recognized as an elicitation effect associated with the use of common nettle extract. The organically fertilized oregano also contained an increased carvacrol content in the headspace, which resulted in a more intense odor of the dry herbs. The use of organic fertilizers in the cultivation of Origanum vulgare L. contributes to the production of raw material for direct consumption and drying, which has a higher bioactive potential and better organoleptic properties.
Natalia Matłok; Agnieszka Ewa Stępień; Józef Gorzelany; Renata Wojnarowska-Nowak; Maciej Balawejder. Effects of Organic and Mineral Fertilization on Yield and Selected Quality Parameters for Dried Herbs of Two Varieties of Oregano (Origanum vulgare L.). Applied Sciences 2020, 10, 5503 .
AMA StyleNatalia Matłok, Agnieszka Ewa Stępień, Józef Gorzelany, Renata Wojnarowska-Nowak, Maciej Balawejder. Effects of Organic and Mineral Fertilization on Yield and Selected Quality Parameters for Dried Herbs of Two Varieties of Oregano (Origanum vulgare L.). Applied Sciences. 2020; 10 (16):5503.
Chicago/Turabian StyleNatalia Matłok; Agnieszka Ewa Stępień; Józef Gorzelany; Renata Wojnarowska-Nowak; Maciej Balawejder. 2020. "Effects of Organic and Mineral Fertilization on Yield and Selected Quality Parameters for Dried Herbs of Two Varieties of Oregano (Origanum vulgare L.)." Applied Sciences 10, no. 16: 5503.
Introduction. Given its very strong antioxidant properties, Moringa oleifera is particularly noteworthy among medicinal plants. The high contents antioxidants in the M. oleifera determining her antioxidant activities deciding for very important anticancer properties. Aim. The aim of the paper is to provide an overview of the cytotoxic and anticancer activity of Moringa oleifera. Material and methods. This review was performed based systematic analysis of literature. Analysis of the literature. The results of scientific research conducted in vitro indicate that extracts from Moringa oleifera significantly affect the development of human cancer cells such as myeloma, leukemia, cervix, breast, colon, lung, liver, neuroblastoma, pancreas, colorectal, epidermoid, oral, ovarian, muscular, prostate, skin. Conclusion. This indicates Moringa oleifera as that they may be used as a therapeutic agent to support oncological therapies.
Kinga Szlachetka; Paulina Kut; Agnieszka Stępień. Cytotoxic and anticancer activity of Moringa oleifera. European Journal of Clinical and Experimental Medicine 2020, 18, 214 -220.
AMA StyleKinga Szlachetka, Paulina Kut, Agnieszka Stępień. Cytotoxic and anticancer activity of Moringa oleifera. European Journal of Clinical and Experimental Medicine. 2020; 18 (3):214-220.
Chicago/Turabian StyleKinga Szlachetka; Paulina Kut; Agnieszka Stępień. 2020. "Cytotoxic and anticancer activity of Moringa oleifera." European Journal of Clinical and Experimental Medicine 18, no. 3: 214-220.
This study investigated the effects of sustainable, organic and standard mineral fertilization in selected phytometric features and contents of bioactive compounds in dry matter of two varieties of Ocimum basilicum L. The herbal material was first examined for its phytometric characterisation and then subjected to the combined convective pre-drying and vacuum-microwave finish drying method (CPD-VMFD). The energy consumption for the drying process of plant material in the case of CPD-VMFD is lower in comparison to the convection method (CD). The obtained dry material was assessed for determination of the colour parameters. Next, the analysis to identify the total content of polyphenols and the antioxidant properties (ABTS) was done. The dried material was subjected to head space–solid phase microextraction (HS-SPME) to determine volatile compound content. The herbal material obtained from the basil cultivated with an addition of sustainable, organic fertilizer was found to have a significantly higher content of bioactive compounds than the control, especially of polyphenols and volatile compounds like eucalyptol. Presumably, this is an effect of the elicitation process resulting from the fact that extract from common nettle was applied as an organic fertilizer component.
Natalia Matłok; Józef Gorzelany; Agnieszka Ewa Stępień; Adam Figiel; Maciej Balawejder. Effect of Fertilization in Selected Phytometric Features and Contents of Bioactive Compounds in Dry Matter of Two Varieties of Basil (Ocimum basilicum L.). Sustainability 2019, 11, 6590 .
AMA StyleNatalia Matłok, Józef Gorzelany, Agnieszka Ewa Stępień, Adam Figiel, Maciej Balawejder. Effect of Fertilization in Selected Phytometric Features and Contents of Bioactive Compounds in Dry Matter of Two Varieties of Basil (Ocimum basilicum L.). Sustainability. 2019; 11 (23):6590.
Chicago/Turabian StyleNatalia Matłok; Józef Gorzelany; Agnieszka Ewa Stępień; Adam Figiel; Maciej Balawejder. 2019. "Effect of Fertilization in Selected Phytometric Features and Contents of Bioactive Compounds in Dry Matter of Two Varieties of Basil (Ocimum basilicum L.)." Sustainability 11, no. 23: 6590.
This study aimed to investigate the changes in the total polyphenolic content and antioxidant properties after subjecting Pink Rock Rose (Cistus creticus) leaves to three different drying procedures, including convection drying (CD) at 40, 50 and 60 °C; vacuum-microwave drying (VMD) at 240 W microwave power; and combined drying consisting of convective pre-drying at 50 °C followed by vacuum-microwave finish drying at 240 W microwave power (CPD-VMFD). The total polyphenolic content and antioxidant properties (DPPH, ABTS) of leaves subjected to these three drying methods were spectrophotometrically determined. The results show that convection drying at 40 °C and vacuum-microwave drying yielded dried leaves with the highest bioactive potential in terms of the total polyphenol content and antioxidant activity, with the highest and lowest values of final specific energy consumption, respectively. The lowest bioactive potential was found in a product dried at 60 °C, which can be attributed to the possible degradation or changes in polyphenol structures under high temperatures. During the combined treatment (CPD-VMFD), most of the moisture was efficiently removed from the raw material by CPD, whereas the time of drying was significantly reduced by the application of VMFD. Combined drying CPD-VMFD is most suitable for industrial applications as it produces dried leaves with a bioactive potential that is only slightly lower than that achieved with VMD while providing a high-throughput capacity relative to operating costs.
Agnieszka Ewa Stępień; Józef Gorzelany; Natalia Matłok; Krzysztof Lech; Adam Figiel. The effect of drying methods on the energy consumption, bioactive potential and colour of dried leaves of Pink Rock Rose (Cistus creticus). Journal of Food Science and Technology 2019, 56, 2386 -2394.
AMA StyleAgnieszka Ewa Stępień, Józef Gorzelany, Natalia Matłok, Krzysztof Lech, Adam Figiel. The effect of drying methods on the energy consumption, bioactive potential and colour of dried leaves of Pink Rock Rose (Cistus creticus). Journal of Food Science and Technology. 2019; 56 (5):2386-2394.
Chicago/Turabian StyleAgnieszka Ewa Stępień; Józef Gorzelany; Natalia Matłok; Krzysztof Lech; Adam Figiel. 2019. "The effect of drying methods on the energy consumption, bioactive potential and colour of dried leaves of Pink Rock Rose (Cistus creticus)." Journal of Food Science and Technology 56, no. 5: 2386-2394.
Agnieszka Ewa Stępień; David Aebisher; Dorota Bartusik Aebisher. Biological properties of Cistus species. European Journal of Clinical and Experimental Medicine 2018, 16, 127 -132.
AMA StyleAgnieszka Ewa Stępień, David Aebisher, Dorota Bartusik Aebisher. Biological properties of Cistus species. European Journal of Clinical and Experimental Medicine. 2018; 16 (2):127-132.
Chicago/Turabian StyleAgnieszka Ewa Stępień; David Aebisher; Dorota Bartusik Aebisher. 2018. "Biological properties of Cistus species." European Journal of Clinical and Experimental Medicine 16, no. 2: 127-132.
Zuzanna Bober; Agnieszka Ewa Stępień; David Aebisher; Łukasz Ożóg; Dorota Bartusik Aebisher. Fundamentals of the use of Berberis as a medicinal plant. European Journal of Clinical and Experimental Medicine 2018, 16, 41 -46.
AMA StyleZuzanna Bober, Agnieszka Ewa Stępień, David Aebisher, Łukasz Ożóg, Dorota Bartusik Aebisher. Fundamentals of the use of Berberis as a medicinal plant. European Journal of Clinical and Experimental Medicine. 2018; 16 (1):41-46.
Chicago/Turabian StyleZuzanna Bober; Agnieszka Ewa Stępień; David Aebisher; Łukasz Ożóg; Dorota Bartusik Aebisher. 2018. "Fundamentals of the use of Berberis as a medicinal plant." European Journal of Clinical and Experimental Medicine 16, no. 1: 41-46.
Zuzanna Bober; Agnieszka Ewa Stępień; David Aebisher; Łukasz Ożog; Dorota Bartusik Aebisher. Medicinal benefits from the use of Black pepper, Curcuma and Ginger. European Journal of Clinical and Experimental Medicine 2018, 16, 133 -145.
AMA StyleZuzanna Bober, Agnieszka Ewa Stępień, David Aebisher, Łukasz Ożog, Dorota Bartusik Aebisher. Medicinal benefits from the use of Black pepper, Curcuma and Ginger. European Journal of Clinical and Experimental Medicine. 2018; 16 (2):133-145.
Chicago/Turabian StyleZuzanna Bober; Agnieszka Ewa Stępień; David Aebisher; Łukasz Ożog; Dorota Bartusik Aebisher. 2018. "Medicinal benefits from the use of Black pepper, Curcuma and Ginger." European Journal of Clinical and Experimental Medicine 16, no. 2: 133-145.
Agnieszka Ewa Stępień; David Aebisher; Dorota Bartusik Aebisher. Anticancer properties of Viburnum. European Journal of Clinical and Experimental Medicine 2018, 16, 47 -52.
AMA StyleAgnieszka Ewa Stępień, David Aebisher, Dorota Bartusik Aebisher. Anticancer properties of Viburnum. European Journal of Clinical and Experimental Medicine. 2018; 16 (1):47-52.
Chicago/Turabian StyleAgnieszka Ewa Stępień; David Aebisher; Dorota Bartusik Aebisher. 2018. "Anticancer properties of Viburnum." European Journal of Clinical and Experimental Medicine 16, no. 1: 47-52.
Zuzanna Bober; Agnieszka Ewa Stępień; David Aebisher; Łukasz Ożóg; Dorota Bartusik Aebisher. Plants – a source of therapeutic material? European Journal of Clinical and Experimental Medicine 2018, 16, 34 -36.
AMA StyleZuzanna Bober, Agnieszka Ewa Stępień, David Aebisher, Łukasz Ożóg, Dorota Bartusik Aebisher. Plants – a source of therapeutic material? European Journal of Clinical and Experimental Medicine. 2018; 16 (1):34-36.
Chicago/Turabian StyleZuzanna Bober; Agnieszka Ewa Stępień; David Aebisher; Łukasz Ożóg; Dorota Bartusik Aebisher. 2018. "Plants – a source of therapeutic material?" European Journal of Clinical and Experimental Medicine 16, no. 1: 34-36.
Agnieszka E. Stepien; Jacek Zebrowski; Łukasz Piszczyk; Valentina V. Boyko; Sergii V. Riabov; Tatiana Dmitrieva; Vladimir I. Bortnitskiy; Mykhailo Gonchar; Renata Wojnarowska-Nowak; Joanna Ryszkowska. Assessment of the impact of bacteria Pseudomonas denitrificans, Pseudomonas fluorescens, Bacillus subtilis and yeast Yarrowia lipolytica on commercial poly(ether urethanes). Polymer Testing 2017, 63, 484 -493.
AMA StyleAgnieszka E. Stepien, Jacek Zebrowski, Łukasz Piszczyk, Valentina V. Boyko, Sergii V. Riabov, Tatiana Dmitrieva, Vladimir I. Bortnitskiy, Mykhailo Gonchar, Renata Wojnarowska-Nowak, Joanna Ryszkowska. Assessment of the impact of bacteria Pseudomonas denitrificans, Pseudomonas fluorescens, Bacillus subtilis and yeast Yarrowia lipolytica on commercial poly(ether urethanes). Polymer Testing. 2017; 63 ():484-493.
Chicago/Turabian StyleAgnieszka E. Stepien; Jacek Zebrowski; Łukasz Piszczyk; Valentina V. Boyko; Sergii V. Riabov; Tatiana Dmitrieva; Vladimir I. Bortnitskiy; Mykhailo Gonchar; Renata Wojnarowska-Nowak; Joanna Ryszkowska. 2017. "Assessment of the impact of bacteria Pseudomonas denitrificans, Pseudomonas fluorescens, Bacillus subtilis and yeast Yarrowia lipolytica on commercial poly(ether urethanes)." Polymer Testing 63, no. : 484-493.
Agnieszka Ewa Stępień. Cytotoxic and anti-cancer activity of the Cistus species of herbal plants. European Journal of Clinical and Experimental Medicine 2017, 15, 165 -168.
AMA StyleAgnieszka Ewa Stępień. Cytotoxic and anti-cancer activity of the Cistus species of herbal plants. European Journal of Clinical and Experimental Medicine. 2017; 15 (2):165-168.
Chicago/Turabian StyleAgnieszka Ewa Stępień. 2017. "Cytotoxic and anti-cancer activity of the Cistus species of herbal plants." European Journal of Clinical and Experimental Medicine 15, no. 2: 165-168.
The enzymes of l-arginine (further - Arg) metabolism are promising tools for elaboration of selective methods for quantitative Arg analysis. In our study we propose an enzymatic method for Arg assay based on fluorometric monitoring of ammonia, a final product of Arg splitting by human liver arginase I (further - arginase), isolated from the recombinant yeast strain, and commercial urease. The selective analysis of ammonia (at 415nm under excitation at 360nm) is based on reaction with o-phthalaldehyde (OPA) in the presence of sulfite in alkali medium: these conditions permit to avoid the reaction of OPA with any amino acid. A linearity range of the fluorometric arginase-urease-OPA method is from 100nM to 6μМ with a limit of detection of 34nM Arg. The method was used for the quantitative determination of Arg in the pooled sample of blood serum. The obtained results proved to be in a good correlation with the reference enzymatic method and literature data. The proposed arginase-urease-OPA method being sensitive, economical, selective and suitable for both routine and micro-volume formats, can be used in clinical diagnostics for the simultaneous determination of Arg as well as urea and ammonia in serum samples.
Nataliya Stasyuk; Galina Gayda; Hasmik Yepremyan; Agnieszka Ewa Stępień; Mykhailo Gonchar. Fluorometric enzymatic assay of l-arginine. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2017, 170, 184 -190.
AMA StyleNataliya Stasyuk, Galina Gayda, Hasmik Yepremyan, Agnieszka Ewa Stępień, Mykhailo Gonchar. Fluorometric enzymatic assay of l-arginine. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2017; 170 ():184-190.
Chicago/Turabian StyleNataliya Stasyuk; Galina Gayda; Hasmik Yepremyan; Agnieszka Ewa Stępień; Mykhailo Gonchar. 2017. "Fluorometric enzymatic assay of l-arginine." Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 170, no. : 184-190.
Agnieszka Ewa Stępień; Kinga Stawarczyk; Maciej Bilek; Katarzyna Kedziora. Analysis of selected chemical parameters in Piemontese wines. Roczniki Państwowego Zakładu Higieny 2015, 66, 1 .
AMA StyleAgnieszka Ewa Stępień, Kinga Stawarczyk, Maciej Bilek, Katarzyna Kedziora. Analysis of selected chemical parameters in Piemontese wines. Roczniki Państwowego Zakładu Higieny. 2015; 66 (3):1.
Chicago/Turabian StyleAgnieszka Ewa Stępień; Kinga Stawarczyk; Maciej Bilek; Katarzyna Kedziora. 2015. "Analysis of selected chemical parameters in Piemontese wines." Roczniki Państwowego Zakładu Higieny 66, no. 3: 1.
The proposed method determines the activity of cholesterol esterase (CEH) and takes advantage of its ability to catalyze the hydrolysis of cholesterol esters naturally present in human serum. The assay is based on Allain's method of spectrophotometric determination of cholesterol by means of cholesterol oxidase, peroxidase, but using 3,5-dichloro-dihydroxybenzenesulfonic acid (DHBS) as phenolic chromogen and human serum as a source of substrate for the CEH as a novelty. Furthermore, it is characterized by low costs and high precision. It can be employed to control the activity of CE preparations used for the preparation of enzymatic kits for the determination of cholesterol or for screening of potential bacterial enzyme producers.
Agnieszka Ewa Stępień; Mykhailo Gonchar. A simple method for the determination of the cholesterol esterase activity. Acta Biochimica Polonica 2013, 60, 1 .
AMA StyleAgnieszka Ewa Stępień, Mykhailo Gonchar. A simple method for the determination of the cholesterol esterase activity. Acta Biochimica Polonica. 2013; 60 (3):1.
Chicago/Turabian StyleAgnieszka Ewa Stępień; Mykhailo Gonchar. 2013. "A simple method for the determination of the cholesterol esterase activity." Acta Biochimica Polonica 60, no. 3: 1.
Agnieszka Ewa Stepien. Assessment of the effect of biodegradation on the chemical structure of poly(ether urethanes). Polimery 2012, 57, 5454 -550.
AMA StyleAgnieszka Ewa Stepien. Assessment of the effect of biodegradation on the chemical structure of poly(ether urethanes). Polimery. 2012; 57 (7):5454-550.
Chicago/Turabian StyleAgnieszka Ewa Stepien. 2012. "Assessment of the effect of biodegradation on the chemical structure of poly(ether urethanes)." Polimery 57, no. 7: 5454-550.
Agnieszka Ewa Stepien. Microbiological degradation of polyurethanes. Polimery 2011, 56, 716 -720.
AMA StyleAgnieszka Ewa Stepien. Microbiological degradation of polyurethanes. Polimery. 2011; 56 (10):716-720.
Chicago/Turabian StyleAgnieszka Ewa Stepien. 2011. "Microbiological degradation of polyurethanes." Polimery 56, no. 10: 716-720.
Agnieszka Ewa Stepien. Biodegradation of poly(ether urethane). Polimery 2010, 55, 431 -434.
AMA StyleAgnieszka Ewa Stepien. Biodegradation of poly(ether urethane). Polimery. 2010; 55 (6):431-434.
Chicago/Turabian StyleAgnieszka Ewa Stepien. 2010. "Biodegradation of poly(ether urethane)." Polimery 55, no. 6: 431-434.