The presented work involves obtaining and characterising a two-enzymatic one-pot bioreactor, including encapsulated (co-immobilised) glucose oxidase and catalase. The enzymatic capsules were applied to produce unsweet, lactose-free milk during low-temperature catalysis. Furthermore, operational conditions, like pH and aeration, were selected in the paper, which sorts out discrepancies in literature reports. All experiments were carried out at 12 °C, corresponding to milk storage and transportation temperature. Preliminary studies (for reasons of analytical accuracy) were carried out in a buffer (pH, concentration of sugars mimicking conditions in the lactose-free milk, the initial glucose concentration 27.5 g/L) verified by processes carried out in milk in the final stage of the study. The presented results showed the need for regulating pH and the aeration of the reaction mixture in the continuous mode during the process. The procedure of co-immobilisation was performed in an alginate matrix with the cross-linking of glutaraldehyde or carbodiimide while carbodiimide showed better enzymes retention inside alginate capsules. Co-encapsulated enzymes could be used for nine cycles, preserving finally about 40% of the initial activity.

Increasingly intense changes in climatic conditions and the use of modified materials are causing fires, the consequences of which are increasingly serious for the environment. On one hand, there is the issue of access to water resources. On the other hand, there is the problem of post-fire wastewater, which often contains a mixture of simple inorganic compounds and complex organic molecules, making the removal of pollutants a difficult task requiring innovative approaches. Among these solutions, hydrogels stand out as a promising class of sorption materials. Depending on their synthesis or functionalization, hydrogels can effectively capture contaminants and facilitate the reduction or removal of specific pollutants. This study explores the functionalization of polymeric materials, specifically hydrogels, using microorganisms or bioactive substances to create materials capable of treating water contaminated with hazardous substances generated during firefighting incidents. The possibility of wastewater capture was also taken into account to retain pretreated water at the place of pollutant generation. The analysis covered the potential, conditions, and limitations of using hydrogels in post-fire operations for the effective management of contaminated waters. It was shown that hydrogels, depending on the modification, have the potential to capture wastewater and purify it from both organic and inorganic substances specific to post-fire wastewater. However, it is not possible for a given hydrogel to meet all desired expectations at the same time. Furthermore, modifications that facilitate the optimal performance of certain functionalities may render the others ineffective.

The current requirements of industrial biocatalysis are related to economically beneficial and environmentally friendly processes. Such a strategy engages low-temperature reactions. The presented approach is essential, especially in food processes, where temperature affects the quality and nutritional value foodstuffs. The subject of the study is the hydrolysis of lactose with the commercial lactase NOLA™ Fit 5500 (NOLA). The complete decomposition of lactose into two monosaccharides gives a sweeter product, recommended for lactose intolerant people and those controlling a product’s caloric content. The hydrolysis reaction was performed at 15 °C, which is related to milk transportation and storage temperature. The enzyme showed activity over the entire range of substrate concentrations (up to 55 g/L lactose). For reusability and easy isolation, the enzyme was encapsulated in a sodium alginate network. Its stability allows carrying out six cycles of the complete hydrolysis of lactose to monosaccharides, lasting from two to four hours. During the study, the kinetic description of native and encapsulated NOLA was conducted. As a result, the model of competitive galactose inhibition and glucose mixed influence (competitive inhibition and activation) was proposed. The capsule size does not influence the reaction rate; thus, the substrate diffusion into capsules can be omitted from the process description. The prepared 4 mm capsules are easy to separate between cycles, e.g., using sieves.

Environmental contamination with heavy metals is inherent in the development of industries. They can be present in municipal and industrial wastewaters, reach biological wastewater treatment plants (WWTPs), and affect the performance of the activated sludge process. Moreover, heavy metals almost never occur in isolation and are components of toxic mixtures. Therefore in this study, the influence of binary metal mixtures Cd–Cu, Cd–Zn, and Cu–Zn on activated sludge dehydrogenase activity was evaluated. Two different models were used to identify possible interactions between mixture components: the concentration addition (CA) model and the independent action (IA) model. It seems that the mode of toxic interaction between mixture components strongly depends on the chosen prediction model and measured endpoint. The joint toxicity of studied mixtures was better described by the IA model compared to the CA model.

The growing need in the current market for innovative solutions to obtain lactose-free (L-F) milk is caused by the annual increase in the prevalence of lactose intolerance inside as well as the newborn, children, and adults. Various configurations of enzymes can yield two distinct L-F products: sweet (β-galactosidase) and unsweet (β-galactosidase and glucose oxidase) L-F milk. In addition, the reduction of sweetness through glucose decomposition should be performed in a one-pot mode with catalase to eliminate product inhibition caused by H₂O₂. Both L-F products enjoy popularity among a rapidly expanding group of consumers. Although enzyme immobilization techniques are well known in industrial processes, new carriers and economic strategies are still being searched. Polymeric carriers, due to the variety of functional groups and non-toxicity, are attractive propositions for individual and co-immobilization of food enzymes. In the presented work, two strategies (with free and immobilized enzymes; β-galactosidase NOLA, glucose oxidase from Aspergillus niger, and catalase from Serratia sp.) for obtaining sweet and unsweet L-F milk under low-temperature conditions were proposed. For free enzymes, achieving the critical assumption, lactose hydrolysis and glucose decomposition occurred after 1 and 4.3 h, respectively. The tested catalytic membranes were created on regenerated cellulose and polyamide. In both cases, the time required for lactose and glucose bioconversion was extended compared to free enzymes. However, these preparations could be reused for up to five (β-galactosidase) and ten cycles (glucose oxidase with catalase). Graphical abstract