Timber structures in marine applications are often exposed to severe degradation conditions caused by mechanical loads and wood-degrading organisms. This paper presents the use of timber in marine environments in Europe from a wood protection perspective. It discusses the use of wood in coastline protection and archeological marine wood, reviews the marine borer taxa in European waters, and gives an overview of potential solutions for protection of timber in marine environments. Information was compiled from the most relevant literature sources with an emphasis on new wood protection methods; the need for research and potential solutions are discussed. Traditionally, timber has been extensively utilized in a variety of marine applications. Although there is a strong need for developing new protection systems for timber in marine applications, the research in this field has been scarce for many years. New attempts to protect timber used in marine environments in Europe have mainly focused on wood modification and the use of mechanical barriers to prevent colonization of marine wood borers. The importance of understanding the mechanisms of settlement, migration, boring, and digestion of the degrading organisms is key for developing effective systems for protecting timber in marine environments.

To evaluate the performance of new wood-based products, reference wood species with known performances are included in laboratory and field trials. However, different wood species vary in their durability performance, and there will also be a within-species variation. The primary aim of this paper was to compare the material resistance against decay fungi and moisture performance of three European reference wood species, i.e., Scots pine sapwood (Pinus sylvestris), Norway spruce (Picea abies), and European beech (Fagus sylvatica). Wood material was collected from 43 locations all over Europe and exposed to brown rot (Rhodonia placenta), white rot (Trametes versicolor) or soft rot fungi. In addition, five different moisture performance characteristics were analyzed. The main results were the two factors accounting for the wetting ability (k_wa) and the inherent protective properties of wood (k_inh), factors for conversion between Norway spruce vs. Scots pine sapwood or European beech for the three decay types and four moisture tests, and material resistance dose (D_Rd) per wood species. The data illustrate that the differences between the three European reference wood species were minor, both with regard to decay and moisture performance. The results also highlight the importance of defined boundaries for density and annual ring width when comparing materials within and between experiments. It was concluded that with the factors obtained, existing, and future test data, where only one or two of the mentioned reference species were used, can be transferred to models and prediction tools that use another of the reference species.

Scots pine exhibits variations in ray anatomy, which are poorly understood. Some ray parenchyma cells develop thick and lignified cell walls before heartwood formation. We hypothesized that some stands and trees show high numbers of lignified and thick-walled parenchyma cells early in the sapwood. Therefore, a microscopic analysis of Scots pine sapwood from four different stands in Northern Europe was performed on Safranin — Astra blue-stained tangential micro sections from outer and inner sapwood areas. Significant differences in lignification and cell wall thickening of ray parenchyma cells were observed in the outer sapwood between all of the stands for the trees analyzed. On a single tree level, the relative lignification and cell wall thickening of ray parenchyma cells ranged from 4.3% to 74.3% in the outer sapwood. In the inner sapwood, lignification and cell wall thickening of ray parenchyma cells were more frequent. In some trees, however, the difference in lignification and cell wall thickening between inner and outer sapwood was small since early lignification, and cell wall thickening was already more common in the outer sapwood. Ray composition and number of rays per area were not significantly different within the studied material. However, only one Scottish tree had a significantly higher number of ray parenchyma cells per ray. The differences discovered in lignification and cell wall thickening in ray parenchyma cells early in the sapwood of Scots pine are relevant for wood utilization in general and impregnation treatments with protection agents in particular.

Extractives from silver birch (Betula pendula) can play an important role in the future bioeconomy by delivering the feedstock, for instance, for antioxidative applications. It is, therefore, inevitable to gain knowledge of the distribution of extractive content and composition in the different tissues of the tree for estimating the potential volumes of valuable extractable compounds. This study examines the extractable compound distribution of different tree tissues such as outer and inner bark and wood, respectively, considering the original height of the stem and comparing the yields after Soxhlet and accelerated solvent extraction (ASE). Eleven parts of the model tree (seven stem discs and four branches) were separated into primary tissues and extracted with a ternary solvent system. The investigated extraction methods resulted in a comparable performance regarding yields and the composition of the extractives. The extractives were divided into single compounds such as betulin, lupeol, γ-sitosterol, and lupeone and substance groups such as carbohydrates, terpenes, aromatics, and other groups. The distribution of single substances and substance groups depends on the location and function of the examined tissues. Furthermore, the evidence for the correlation of a single substance’s location and original tree height is stronger for lupeol than for betulin. Primary betulin sources of the calculated betulin output are the outer bark of the stem and the branches. By using small branches, further potential for the extraction of betulin can be utilized. A model calculation of the betulin content in the current birch tree revealed a significant potential of 23 kg of betulin available as a valuable chemical resource after by-product utilization.

The extractive content of inner and outer heartwood of nine Scots pine trees from three different stands in Norway was determined by automated solvent extraction and biological screening tests were performed using basidiomycetes. The evaluation of mass spectra by means of a NIST library search shows that in the petroleum ether extracts α-pinene and carene as well as terpinene and cadinene derivatives are the main extractives found in both inner and outer heartwood. In the inner heartwood, however, these substance groups were found in lower quantities. These substances mainly have a hydrophobic effect. The screening tests indicate that also extractive-rich heartwood is extremely degraded by Poria placenta which corresponds to the analytical results of the petroleum ether extracts.

This paper aimed to investigate the genetic structure (GS) of Scots pine in the northern area of its distribution range by means of seven neutral nuclear microsatellite markers. In particular, the postglacial recolonization of these areas and possible different adaptation patterns in distinct refugia were studied. The GS and diversity were assessed with seven pairs of neutral nuclear microsatellite primers. A high genetic diversity was found in the Scots pine material tested, along with a shallow GS. This pattern is typical for recolonized areas and species with large population sizes, which are connected by pollen-mediated gene flow. A STRUCTURE analysis found two genetic groups to be the most likely, one south-eastern and one north-western group that meet in Fennoscandia. This indicates that Scots pine recolonization of Fennoscandia might have taken place from two different directions (south-west and north-east). Scots pine that recolonized the area originated in at least two different refugia during the last glacial maximum. The glacial survival in distinct refugia can have led to different adaptation patterns and growth optima in the different groups as reflected in the formation of latewood content, where lineage was a significant influencing factor.