This study reports the mitigating strategy against salinity by exploring the potential effects of biochar (5%), Arbuscular mycorrhizal fungi (20 g/pot, AMF), and biochar + AMF on maize (Zea mays L.) plants grown under saline stress in a greenhouse. The maize was grown on alkaline soil and subjected to four different saline levels; 0, 50, 100, and 150 mM NaCl. After 90 d for 100 mM NaCl treatment, the plant’s height and fresh weight were reduced by 17.84% and 39.28%, respectively, compared to the control. When the saline-treated soil (100 mM NaCl) was amended with AMF, biochar, and biochar + AMF, the growth parameters were increased by 22.04%, 26.97%, 30.92% (height) and 24.79%, 62.36%, and 107.7% (fresh weight), respectively. Compared to the control and single AMF/biochar treatments, the combined application of biochar and AMF showed the most significant effect in improving maize growth under saline stress. The superior mitigating effect of biochar + AMF was attributed to its effective ability in (i) improving soil nutrient content, (ii) enhancing plant nutrient uptake, (iii) increasing the activities of antioxidant enzymes, and (iv improving the contents of palmitoleic acid (C16:1), oleic acid (C18:1), linoleic acid (C18:2), and linolenic acid (C18:3). Thus, our study shows that amending alkaline and saline soils with a combination of biochar-AMF can effectively mitigate abiotic stress and improve plant growth. Therefore, it can serve as a reference for managing salinity stress in agricultural soils.

Salt stress in soils impacts grain crop yield. Soil amendment with biochar and arbuscular mycorrhizal alone has been analyzed to improve the growth of several crops under salinity stress. However, the combined application of biochar and arbuscular mycorrhizal fungi for the remediation of salinity and improvement of crop productivity in wheat are rarely discussed and have remained unclear. Therefore, this experiment was performed to investigate the effect with biochar (150 g biochar per each treated pot containing 3 kg soil) and/or arbuscular mycorrhizal fungi (20 g AMF inoculum containing 80% mycorrhizal roots, 100–160 spores, and extraradical hyphae per each treated pot) on the productivity of wheat (Triticum aestivum L.) under four salt stress gradients; 0, 50, 100, and 150 mM NaCl. The results show salinity significantly reduced plant height (9.9% to 22.9%), shoot fresh weight (35.6% to 64.4%), enzymatic activities (34.1% to 39.3%), and photosynthetic pigments—i.e., total chlorophyll contents (75.0%) and carotenoids contents (56.2%) of plants—as compared with control. Under exclusive biochar application, the plants were moderately tolerant to salinity stress, which was evident in their growth, moderately reduced fatty acid content, partially impaired enzymatic activity, and photosynthetic pigments, while under the exclusive AMF application, the wheat plants were relatively sensitive to salinity stress, resulting in impaired growth rate, decreased unsaturated fatty acid composition, enzymatic activity, and photosynthetic pigments. Conversely, under the co-application of biochar and AMF, wheat plants partially increased plant height (14.1%), shoot fresh biomass (75.7%), root fresh biomass (24.9%), partially increased enzymatic activity (49.5%), and unimpaired photosynthetic pigments (30.2% to 54.8%) of wheat under salinity stress. Current findings concluded that exclusive incorporation of biochar, and the synergistic application of AMF and biochar, could be utilized as a promising way to reduce the deleterious effects of salinity stress in wheat production.