Sugarcane grown under a wide range of agro-climatic conditions accounts for ~80% of the sugar production worldwide. Since sugarcane productivity is severely affected by abiotic stresses and hence, an experiment was conducted for two consecutive years during 2020 and 2021 on popular sub-tropical sugarcane varieties. The experiment was laid out in two-factorial RBD consisting of nine sugarcane genotypes (Co 98014, Co 0118, Co 0238, Co 05011, Co 06034, Co 09022, Co 12029, Co 15023 and Co 15027) and salinity treatments (Control, EC_iw ~ 4, 8 and 12 dS m⁻¹) in 5 replications. Two budded setts were planted in pots and irrigated with saline water of respective levels till formative phase and observed the build-up in electrical conductivity of soil extract (ECse) from 0.48 (control) to 2.99, 4.81 and 7.08; while further saline irrigation increased the ECse values to 4.48, 6.24 and 9.33 dS m⁻¹ in treatments EC_iw ~ 4, 8 and 12 dS m⁻¹, respectively. Increase in soil EC decreased plant survival by 24.1, 47.0 and 79.6% under continued irrigation of EC_iw ~ 4, 8, 12 dS m⁻¹ with respect to control. Continued saline irrigation caused significant reduction in growth, which was associated with reduction in relative water content (RWC) and gas exchange traits. RWC decreased by 4.91 to 21.9%, chlorophyll content by 8.46 to 32.75%, photosynthetic rate (Pn) by 16.85 to 91.44%, stomatal conductance by 14.96 to 84.25%, transpiration rate by 14.13% to 89.8% and chlorophyll fluorescence by 5.33 to 42.67% from EC_iw ~ 4 to 12 dS m⁻¹, respectively. Significant variations in Na⁺ and K⁺ ion content was observed under elevated saline condition in roots, leaves and juice extract of genotypes. Na⁺/K⁺ ratio, an important trait for screening salinity tolerance, increased in all genotypes as compared to control, the increase was predominant in susceptible varieties. Single cane weight (SCW) was drastically affected by saline irrigation, with a reduction of 36.4, 68.5 and 83.5% at EC_iw ~ 4, 8 and 12 dS m⁻¹, respectively as compared to control, with similar declining trend in juice quality. Based on our results, Co 0238, Co 0118 and Co 98014 were tolerant to salinity stress by maintaining higher Pn, lower leaf Na⁺/K⁺ ratio, higher SCW and higher juice sucrose content.

Drought and salinity individually or in combination adversely affects growth, development, and yield of sugarcane. Apart from physiological traits, nutrients status was studied in six commercial hybrids subjected to drought, salinity, and salinity + drought. Drought was simulated by withholding irrigation in the field, while salinity and salinity + drought were imposed in microplots. Physiological traits, viz, chlorophyll fluorescence, chlorophyll (SPAD) index, biomass production, and leaf area index, reduced drastically in combined stress as against individual stress treatments. Among major nutrients, phosphorus (P) reduced significantly in all the stress treatments. Similarly, iron (Fe) and Zinc (Zn) reduced due to stress and showed differential response for growth stages. Reduction in cane yield and sucrose percentage in stress conditions warrants supplementation of P, Fe, and Zn.

Screening for elite sugarcane genotypes for canopy cover in a rapid and non-destructive way is important to accelerate varietal/clonal selection, and little information is available regarding canopy cover and leaf production, leaf area, biomass production, and cane yield in sugarcane crop. In the present investigation, the digital images of sugarcane crop by using Canopeo software was assessed for their correlation with the physiological and morphological parameters and cane yield production. The results revealed that among the studied parameters, canopy coverage has shown a significantly better correlation with the plant height (0.581 **), leaf length (0.853 **), leaf width (0.587 **), and leaf area (0.770 **) in commercial sugarcane clones. Two-way cluster analysis has led to the identification of Co 0238, Co 86249, Co 10026, Co 99004, Co 94008, and Co 95020 with better physiological traits for higher sugarcane yield under changing climate. Additionally, in another field experiment with pre-breeding, germplasm, and interspecific hybrid sugarcane clones, the canopy coverage showed a significantly better correlation with germination, shoot count, leaf weight, leaf area index, and plant height, and finally with biomass (r = 0.612 **) and cane yield (r = 0.458 **). It has been found that the plant height, total dry matter (TDM), and leaf area index (LAI) had significant correlation with the cane yield, and the canopy cover data from digital images act as a surrogate for these traits, and further it has been observed that CC had better correlation with cane yield compared to the other physiological traits viz., SPAD, total chlorophyll (TC), and canopy temperature (CT) under ambient conditions. Light interception determined using a line quantum sensor had a significant positive correlation (r = 0.764 **) with canopy coverage, signifying the importance of determining the latter in a non-destructive way in a rapid manner and low cost.