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Prof. Hideyuki Kanematsu
National Institute of Technology, Suzuka College

Basic Info


Research Keywords & Expertise

0 Biofilms
0 Electrochemistry
0 EPS
0 Gear
0 Learning

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biofilm
Biofilm formation
Biofilms
Learning
Second Life
Raman Spectroscopy
STEM education
biofouling
EPS
Metaverse
Nuclear Safety
Eye Blinking
Electrochemistry
corrosion science
External polymeric substances

Honors and Awards

Scientific Achievement Award

Research Activities for heat surface treatments

National Association for Surface Finishing Society (NASF, the USA)


Molten Salt Award

Application of molten salt technology to various materials processing and its development .

Molten Salt Committee, the Electrochemical Society of Japan


Outstanding Achievement Award

The presentation entitle Grime on materials’ surfaces and Biofilms was evaluated and awarded.

MRS-Japan




Career Timeline

National Insitute of Technology (KOSEN), Suzuka College

University Educator/Researcher

01 April 1992 - 31 August 2021


Osaka University

University Educator/Researcher

01 March 1990 - 01 March 1992


Nagoya University, Japan

University Educator/Researcher

01 April 1986 - 01 February 1990




Short Biography

Dr. Hideyuki Kanematsu, FIMF (a Fellow of IMF) is a full professor in the Department of Materials Science and Engineering, at National Institute of Technology (KOSEN), Suzuka College (NIT (KOSEN), Suzuka College), Japan and also the Unit Leader (Director) of KOSEN GEAR Project (KOSEN Research Center of Materials Science). After many administration positions in the college (Dean of Dept. MS& E, 2010-2014, Deputy President, 2014-2018, etc.), he is also still a researcher in Materials Surface Science & Engineering and the research advisor of the college. He holds a B.Eng. (1981), a M.Eng(1983) and a Ph.D in Materials Science and Engineering (1989) all from Nagoya University. He is a member of NASF, TMS, ASM International ECS, ACS (USA), JIM, ISIJ, SFSJ, ECSJ, MRS-J , JSHT, JTSS (Japan), etc. For all of them, he is still very active as member and also as board member.

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Project

Project Goal: The completion of anti-virus coating and anti-virus materials.

Starting Date:01 April 2020

Current Stage: The project had just begun.

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Project

Project Goal: The clarification of mechanism inside biofilms and the application to biofilm sensors.

Starting Date:07 January 2019

Current Stage: The signals applicable to sensors were detected.

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Project

Project Goal: The completion of graphene biofilm sensor

Starting Date:01 April 2017

Current Stage: My colleagues and I found the signal from graphene to control biofilms.

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Project

Project Goal: The completion of staining and evaluation technique with crystal biolet and the standardization of the method.

Starting Date:10 April 2015

Current Stage: The method is going to be applied to ISO standarization.

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Project

Project Goal: The establishment of mechanism and technology

Starting Date:01 April 2013

Current Stage: The technology is applied to many industries. I focuse on the clarification of mechanism.

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Journal article
Published: 30 May 2021 in ECS Meeting Abstracts
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ACS Style

Hideyuki Kanematsu; Ryoichi Nakagawa; Yuki Torisawa; Hidekazu Miura; Masatou Ishihara; Masahito Ban; Nobumitsu Hirai; Takeshi Kogo; Dana M. Barry. Impedance Characteristics of Biofilms Formed on Graphene Films and Their Substrates. ECS Meeting Abstracts 2021, MA2021-01, 657 -657.

AMA Style

Hideyuki Kanematsu, Ryoichi Nakagawa, Yuki Torisawa, Hidekazu Miura, Masatou Ishihara, Masahito Ban, Nobumitsu Hirai, Takeshi Kogo, Dana M. Barry. Impedance Characteristics of Biofilms Formed on Graphene Films and Their Substrates. ECS Meeting Abstracts. 2021; MA2021-01 (14):657-657.

Chicago/Turabian Style

Hideyuki Kanematsu; Ryoichi Nakagawa; Yuki Torisawa; Hidekazu Miura; Masatou Ishihara; Masahito Ban; Nobumitsu Hirai; Takeshi Kogo; Dana M. Barry. 2021. "Impedance Characteristics of Biofilms Formed on Graphene Films and Their Substrates." ECS Meeting Abstracts MA2021-01, no. 14: 657-657.

Journal article
Published: 30 May 2021 in ECS Meeting Abstracts
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ACS Style

Hideyuki Kanematsu; Sho Ogaki; Noe Sugino; Nobumitsu Hirai; Noriyuki Wada; Takeshi Kogo; Hirohisa Yamada; Katsuhiko Tsunashima; Dana M. Barry. Polarization Behaviors of Biofilms on Metallic Materials By E.coli and S.Epidermidis, and the Applicability of Results. ECS Meeting Abstracts 2021, MA2021-01, 1743 -1743.

AMA Style

Hideyuki Kanematsu, Sho Ogaki, Noe Sugino, Nobumitsu Hirai, Noriyuki Wada, Takeshi Kogo, Hirohisa Yamada, Katsuhiko Tsunashima, Dana M. Barry. Polarization Behaviors of Biofilms on Metallic Materials By E.coli and S.Epidermidis, and the Applicability of Results. ECS Meeting Abstracts. 2021; MA2021-01 (42):1743-1743.

Chicago/Turabian Style

Hideyuki Kanematsu; Sho Ogaki; Noe Sugino; Nobumitsu Hirai; Noriyuki Wada; Takeshi Kogo; Hirohisa Yamada; Katsuhiko Tsunashima; Dana M. Barry. 2021. "Polarization Behaviors of Biofilms on Metallic Materials By E.coli and S.Epidermidis, and the Applicability of Results." ECS Meeting Abstracts MA2021-01, no. 42: 1743-1743.

Editorial
Published: 24 July 2020 in Coatings
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Based on five Special Issues in Coatings, this e-book contains a series of fifteen articles demonstrating actual perspectives and new trends in advanced coatings in buildings. Innovative materials and multiperformance solutions provide a basis, contributing also to better protection of buildings’ surfaces during the service life, and users’ wellbeing.

ACS Style

Aníbal Maury-Ramírez; Inês Flores-Colen; Hideyuki Kanematsu. Advanced Coatings for Buildings. Coatings 2020, 10, 728 .

AMA Style

Aníbal Maury-Ramírez, Inês Flores-Colen, Hideyuki Kanematsu. Advanced Coatings for Buildings. Coatings. 2020; 10 (8):728.

Chicago/Turabian Style

Aníbal Maury-Ramírez; Inês Flores-Colen; Hideyuki Kanematsu. 2020. "Advanced Coatings for Buildings." Coatings 10, no. 8: 728.

Journal article
Published: 01 May 2020 in ECS Meeting Abstracts
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ACS Style

Hideyuki Kanematsu; Reo Itoh; Sho Ogaki; Nobumitsu Hirai; Takeshi Kogo; Akiko Ogawa; Noriyuki Wada; Katsuhiko Tsunashima; Dana M. Barry. Electrochemical Monitoring of Metallic Materials' Surfaces with Biofilm Formation. ECS Meeting Abstracts 2020, MA2020-01, 2525 -2525.

AMA Style

Hideyuki Kanematsu, Reo Itoh, Sho Ogaki, Nobumitsu Hirai, Takeshi Kogo, Akiko Ogawa, Noriyuki Wada, Katsuhiko Tsunashima, Dana M. Barry. Electrochemical Monitoring of Metallic Materials' Surfaces with Biofilm Formation. ECS Meeting Abstracts. 2020; MA2020-01 (44):2525-2525.

Chicago/Turabian Style

Hideyuki Kanematsu; Reo Itoh; Sho Ogaki; Nobumitsu Hirai; Takeshi Kogo; Akiko Ogawa; Noriyuki Wada; Katsuhiko Tsunashima; Dana M. Barry. 2020. "Electrochemical Monitoring of Metallic Materials' Surfaces with Biofilm Formation." ECS Meeting Abstracts MA2020-01, no. 44: 2525-2525.

Journal article
Published: 01 May 2020 in ECS Meeting Abstracts
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Biofilms formed on materials have brought about many industrial problems so far. Chronic diseases, the deterioration of hygiene conditions etc. could be mentioned for example. We have confirmed that graphene is easy to form biofilms on it in various ways. Therefore, we expect and predict it could be applied to a practical sensor for biofilm detections. In our precious studies, we produced mono layer graphene on glass specimens and confirmed that it was highly sensitive to biofilm formation in a flow type laboratory biofilm reactor. However, monolayer graphene is generally still lacking in uniformity and the non-coating area is often observed. Therefore, we investigated the sensitivity of two layer CVD graphene to biofilm formation and the possibility to biofilm sensor. In this study, we focused on the change of capacitance with biofilm formation on the graphene coating. The graphene coating specimens on copper and PET substrates were set to laboratory biofilm reactors and we formed biofilms on the specimens artificially. After the formation of biofilms, the specimens were checked by Raman spectroscopy and also observed by an optical microscopy. And the specimens' capacitances were measured, using a specially devised jig connecting to a LCR meter. The frequencies were changed from 700 to 2000Hz and the capacitances were measured. As a result, biofilm formed specimens showed small peaks at some characteristics frequencies, while specimens without biofilms showed no peaks at all. Therefore, we presumed that dielectric polarization-relaxation and the loss would occur due to the existence of External Polymeric Substances (EPS) derived from biofilms. We discussed the application possibility of this phenomenon to biofilm sensing.

ACS Style

Hideyuki Kanematsu; Ryoichi Nakagawa; Kai Saito; Hidekazu Miura; Masatou Ishihara; Masato Ban; Nobumitsu Hirai; Takeshi Kogo; Akiko Ogawa; Dana M. Barry. Biofilm Formation on Two Layer CVD Graphene and Its Change of Capacitance. ECS Meeting Abstracts 2020, MA2020-01, 2282 -2282.

AMA Style

Hideyuki Kanematsu, Ryoichi Nakagawa, Kai Saito, Hidekazu Miura, Masatou Ishihara, Masato Ban, Nobumitsu Hirai, Takeshi Kogo, Akiko Ogawa, Dana M. Barry. Biofilm Formation on Two Layer CVD Graphene and Its Change of Capacitance. ECS Meeting Abstracts. 2020; MA2020-01 (30):2282-2282.

Chicago/Turabian Style

Hideyuki Kanematsu; Ryoichi Nakagawa; Kai Saito; Hidekazu Miura; Masatou Ishihara; Masato Ban; Nobumitsu Hirai; Takeshi Kogo; Akiko Ogawa; Dana M. Barry. 2020. "Biofilm Formation on Two Layer CVD Graphene and Its Change of Capacitance." ECS Meeting Abstracts MA2020-01, no. 30: 2282-2282.

Journal article
Published: 01 May 2020 in ECS Meeting Abstracts
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ACS Style

Hideyuki Kanematsu; Atsuya Oizumi; Takaya Sato; Ryo Sato; Toshio Kamijo; Saika Honma; Nobumitsu Hirai; Takeshi Kogo; Akiko Ogawa; Katsuhiko Tsunashima; Dana M. Barry. Polymer Brush Made from Ionic Liquid and Its Anti-Biofilm Formation Behaviors By Environmental Biota in a Flow-Type Laboratory Biofilm Reactor. ECS Meeting Abstracts 2020, MA2020-01, 2519 -2519.

AMA Style

Hideyuki Kanematsu, Atsuya Oizumi, Takaya Sato, Ryo Sato, Toshio Kamijo, Saika Honma, Nobumitsu Hirai, Takeshi Kogo, Akiko Ogawa, Katsuhiko Tsunashima, Dana M. Barry. Polymer Brush Made from Ionic Liquid and Its Anti-Biofilm Formation Behaviors By Environmental Biota in a Flow-Type Laboratory Biofilm Reactor. ECS Meeting Abstracts. 2020; MA2020-01 (43):2519-2519.

Chicago/Turabian Style

Hideyuki Kanematsu; Atsuya Oizumi; Takaya Sato; Ryo Sato; Toshio Kamijo; Saika Honma; Nobumitsu Hirai; Takeshi Kogo; Akiko Ogawa; Katsuhiko Tsunashima; Dana M. Barry. 2020. "Polymer Brush Made from Ionic Liquid and Its Anti-Biofilm Formation Behaviors By Environmental Biota in a Flow-Type Laboratory Biofilm Reactor." ECS Meeting Abstracts MA2020-01, no. 43: 2519-2519.

Journal article
Published: 19 February 2020 in Materials
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In this study, we examined the relationship between the effect of a zinc coating on protecting carbon steel against biofilm formation in both air and water environments. SS400 carbon steel coupons were covered with a zinc thermal spray coating or copper thermal spray coating. Coated coupons were exposed to either air or water conditions. Following exposure, the surface conditions of each coupon were observed using optical microscopy, and quantitatively analyzed using an x-ray fluorescence analyzer. Debris on the surface of the coupons was used for biofilm analysis including crystal violet staining for quantification, Raman spectroscopic analysis for qualification, and microbiome analysis. The results showed that the zinc thermal spray coating significantly inhibited iron corrosion as well as biofilm formation in both air and water environments. The copper thermal spray coating, however, accelerated iron corrosion in both air and water environments, but accelerated biofilm formation only in a water environment. microbially-influenced-corrosion-related bacteria were barely detected on any coupons, whereas biofilms were detected on all coupons. To summarize these results, electrochemical corrosion is dominant in an air environment and microbially influenced corrosion is strongly involved in water corrosion. Additionally, biofilm formation plays a crucial rule in carbon steel corrosion in both air and water, even though microbially-influenced-corrosion-related bacteria are barely involved in this corrosion.

ACS Style

Akiko Ogawa; Keito Takakura; Nobumitsu Hirai; Hideyuki Kanematsu; Daisuke Kuroda; Takeshi Kougo; Katsuhiko Sano; Satoshi Terada. Biofilm Formation Plays a Crucial Rule in the Initial Step of Carbon Steel Corrosion in Air and Water Environments. Materials 2020, 13, 923 .

AMA Style

Akiko Ogawa, Keito Takakura, Nobumitsu Hirai, Hideyuki Kanematsu, Daisuke Kuroda, Takeshi Kougo, Katsuhiko Sano, Satoshi Terada. Biofilm Formation Plays a Crucial Rule in the Initial Step of Carbon Steel Corrosion in Air and Water Environments. Materials. 2020; 13 (4):923.

Chicago/Turabian Style

Akiko Ogawa; Keito Takakura; Nobumitsu Hirai; Hideyuki Kanematsu; Daisuke Kuroda; Takeshi Kougo; Katsuhiko Sano; Satoshi Terada. 2020. "Biofilm Formation Plays a Crucial Rule in the Initial Step of Carbon Steel Corrosion in Air and Water Environments." Materials 13, no. 4: 923.

Book chapter
Published: 08 June 2019 in Handbook of Nanofibers
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In this chapter biofilms are introduced. Also their relationship with nanofibers is described from the viewpoint of materials science. To start, the background information for this topic is presented and explained. Also we show how biofilm causes industrial problems. The relationships of biofilms to nanofibers are classified in two main ways. One of them is the bacterial nanofiber which they produce by themselves. The other refers to the role of the fibers. A fiber seems to control the shape of biofilms which the aggregation of bacteria could produce. On the other hand, another fiber could play an important role for the attachment of bacteria onto material surfaces. Therefore, all of the mentioned examples would lead to the surface phenomena occurring on material surfaces. Finally, we present and describe a polymer brush coating as a countermeasure against biofilm formation.

ACS Style

Hideyuki Kanematsu; Dana M. Barry; Hajime Ikegai; Yoshimitsu Mizunoe; Michiko Yoshitake. Nanofibers and Biofilm in Materials Science. Handbook of Nanofibers 2019, 389 -408.

AMA Style

Hideyuki Kanematsu, Dana M. Barry, Hajime Ikegai, Yoshimitsu Mizunoe, Michiko Yoshitake. Nanofibers and Biofilm in Materials Science. Handbook of Nanofibers. 2019; ():389-408.

Chicago/Turabian Style

Hideyuki Kanematsu; Dana M. Barry; Hajime Ikegai; Yoshimitsu Mizunoe; Michiko Yoshitake. 2019. "Nanofibers and Biofilm in Materials Science." Handbook of Nanofibers , no. : 389-408.

Original article
Published: 01 June 2019 in MEDICAL DEVICES & SENSORS
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Biofilm is a substance that contains organic polymers. It is derived from bacteria, produces slime and forms on material's surfaces. Therefore, it is important to properly evaluate biofilm formation using several types of sensors. Since biofilm forms easily on graphene, it is possible for graphene to serve as a sensor for biofilm formation. In the past, graphene's sensitivity to biofilm formation was confirmed by using CVD (chemical vapour deposition) graphene and by using the tape‐exfoliation method. However, the CVD method was expensive and required special equipment. On the other hand, the exfoliation method was not so expensive, but the precision and reproducibility for graphene formation were problematic. In this experiment, silane‐based resin, with dispersed graphene, was coated on a substrate. This coated substrate can easily be used as a sensor and is an inexpensive way to promote biofilm formation. Glass was used as the substrate, for which various samples were prepared using different concentrations of dispersed graphene. Biofilms formed on the specimens’ surfaces were analysed by using Raman spectroscopy and the crystal violet staining method. The results showed that biofilm formation capability increased with the concentration of dispersed graphene. We confirmed that the sensitivity was evaluated by the colour value for stained surfaces by crystal violet.

ACS Style

Hideyuki Kanematsu; Ryoichi Nakagawa; Katsuhiko Sano; Dana M. Barry; Akiko Ogawa; Nobumitsu Hirai; Takeshi Kogo; Daisuke Kuroda; Noriyuki Wada; Seung‐Hyo Lee; Yoshimitsu Mizunoe. Graphene‐dispersed silane compound used as a coating to sense immunity from biofilm formation. MEDICAL DEVICES & SENSORS 2019, 2, 1 .

AMA Style

Hideyuki Kanematsu, Ryoichi Nakagawa, Katsuhiko Sano, Dana M. Barry, Akiko Ogawa, Nobumitsu Hirai, Takeshi Kogo, Daisuke Kuroda, Noriyuki Wada, Seung‐Hyo Lee, Yoshimitsu Mizunoe. Graphene‐dispersed silane compound used as a coating to sense immunity from biofilm formation. MEDICAL DEVICES & SENSORS. 2019; 2 (3-4):1.

Chicago/Turabian Style

Hideyuki Kanematsu; Ryoichi Nakagawa; Katsuhiko Sano; Dana M. Barry; Akiko Ogawa; Nobumitsu Hirai; Takeshi Kogo; Daisuke Kuroda; Noriyuki Wada; Seung‐Hyo Lee; Yoshimitsu Mizunoe. 2019. "Graphene‐dispersed silane compound used as a coating to sense immunity from biofilm formation." MEDICAL DEVICES & SENSORS 2, no. 3-4: 1.

Journal article
Published: 30 May 2019 in Scientific Reports
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A biofilm has a unique structure composed of microorganisms, extracellular polymeric substances (EPSs), etc., and it is layered on a substrate in water. In material science, it is important to detect the biofilm formed on a surface to prevent biofouling. EPSs, the major component of the biofilm, mainly consist of polysaccharides, proteins, nucleic acids, and lipids. Because these biomolecules have a variety of hydrophilicities or hydrophobicities, the substrate covered with the biofilm shows different wettability from the initial state. To detect the biofilm formation, this study employed a liquid-squeezing-based wettability assessment method with a simple wettability index: the liquid-squeezed diameter of a smaller value indicates higher wettability. The method is based on the liquid-squeezing behaviour of a liquid that covers sample surfaces when an air-jet is applied. To form the biofilm, polystyrene surfaces were immersed and incubated in a water-circulated bioreactor that had collected microorganisms in ambient air. After the 14-d incubation, good formation of the biofilm on the surfaces was confirmed by staining with crystal violet. Although the contact angles of captive bubbles on the surfaces with the biofilm were unmeasurable, the liquid-squeezing method could distinguish between hydrophilic and hydrophobic initial surfaces with and without biofilm formation using the diameter of the liquid-squeezed area. The surface wettability is expected to be a promising property for in-situ detection of biofilm formation on a macroscopic scale.

ACS Style

Nobuyuki Tanaka; Takeshi Kogo; Nobumitsu Hirai; Akiko Ogawa; Hideyuki Kanematsu; Junko Takahara; Akane Awazu; Nobuko Fujita; Yoshihide Haruzono; Shunji Ichida; Yo Tanaka. In-situ detection based on the biofilm hydrophilicity for environmental biofilm formation. Scientific Reports 2019, 9, 1 -11.

AMA Style

Nobuyuki Tanaka, Takeshi Kogo, Nobumitsu Hirai, Akiko Ogawa, Hideyuki Kanematsu, Junko Takahara, Akane Awazu, Nobuko Fujita, Yoshihide Haruzono, Shunji Ichida, Yo Tanaka. In-situ detection based on the biofilm hydrophilicity for environmental biofilm formation. Scientific Reports. 2019; 9 (1):1-11.

Chicago/Turabian Style

Nobuyuki Tanaka; Takeshi Kogo; Nobumitsu Hirai; Akiko Ogawa; Hideyuki Kanematsu; Junko Takahara; Akane Awazu; Nobuko Fujita; Yoshihide Haruzono; Shunji Ichida; Yo Tanaka. 2019. "In-situ detection based on the biofilm hydrophilicity for environmental biofilm formation." Scientific Reports 9, no. 1: 1-11.

Journal article
Published: 13 November 2018 in Coatings
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N,N-diethyl-N-(2-methancryloylethy)-N-methylammonium bis(trifluoromethylsulfonyl) imide polymer (DEMM-TFSI) brush coated specimens (substrate: glasses) and a liquid ion type of polymer brush coating were investigated for their antifouling effect on biofilms. Biofilms were produced by two kinds of bacteria, E. coli and S. epidermidis. They were formed on specimens immersed into wells (of 12-well plates) that were filled with culture liquids and bacteria. The biofilm formation was observed. Also, brush coated specimens and glass substrates were investigated in the same way. DEMM polymer brush coated specimens formed more biofilm than PMMA (polymethyl methacrylate) polymer brush coated specimens and glass substrates. A greater amount of polarized components of biofilms was also observed for DEMM polymer brush coated specimens. The polar characteristics could be attributed to the attraction capability of bacteria and biofilms on DEMM polymer brush coated specimens. When considering the ease of removing biofilms by washing it with water, the ionic liquid type polymer brush (coated specimens) could be used for antifouling applications. If an initial antifouling application is needed, then the polar characteristics could be adjusted (design of the components and concentrations of ionic liquids, etc.) to solve the problem.

ACS Style

Hideyuki Kanematsu; Atsuya Oizumi; Takaya Sato; Toshio Kamijo; Saika Honma; Dana M. Barry; Nobumitsu Hirai; Akiko Ogawa; Takeshi Kogo; Daisuke Kuroda; Katsuhiko Sano; Katsuhiko Tsunashima; Seung-Hyo Lee; Myeong-Hoon Lee. Biofilm Formation of a Polymer Brush Coating with Ionic Liquids Compared to a Polymer Brush Coating with a Non-Ionic Liquid. Coatings 2018, 8, 398 .

AMA Style

Hideyuki Kanematsu, Atsuya Oizumi, Takaya Sato, Toshio Kamijo, Saika Honma, Dana M. Barry, Nobumitsu Hirai, Akiko Ogawa, Takeshi Kogo, Daisuke Kuroda, Katsuhiko Sano, Katsuhiko Tsunashima, Seung-Hyo Lee, Myeong-Hoon Lee. Biofilm Formation of a Polymer Brush Coating with Ionic Liquids Compared to a Polymer Brush Coating with a Non-Ionic Liquid. Coatings. 2018; 8 (11):398.

Chicago/Turabian Style

Hideyuki Kanematsu; Atsuya Oizumi; Takaya Sato; Toshio Kamijo; Saika Honma; Dana M. Barry; Nobumitsu Hirai; Akiko Ogawa; Takeshi Kogo; Daisuke Kuroda; Katsuhiko Sano; Katsuhiko Tsunashima; Seung-Hyo Lee; Myeong-Hoon Lee. 2018. "Biofilm Formation of a Polymer Brush Coating with Ionic Liquids Compared to a Polymer Brush Coating with a Non-Ionic Liquid." Coatings 8, no. 11: 398.

Journal article
Published: 22 October 2018 in Antibiotics
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Previously, we demonstrated that silver nanoparticle-dispersed silane-based coating could inhibit biofilm formation in conditions where seawater was used as a bacterial source and circulated in a closed laboratory biofilm reactor. However, it is still unclear whether the microbiome of a biofilm of silver nanoparticle-dispersed silane-based coating samples (Ag) differs from that of a biofilm of non-dispersed silane-based coating samples (Non-Ag). This study aimed to perform a microbiome analysis of the biofilms grown on the aforementioned coatings using a next-generation sequencing (NGS) technique. For this, a biofilm formation test was conducted by allowing seawater to flow through a closed laboratory biofilm reactor; subsequently, DNAs extracted from the biofilms of Ag and Non-Ag were used to prepare 16S rRNA amplicon libraries to analyze the microbiomes by NGS. Results of the operational taxonomy unit indicated that the biofilms of Non-Ag and Ag comprised one and no phyla of archaea, respectively, whereas Proteobacteria was the dominant phylum for both biofilms. Additionally, in both biofilms, Non-Ag and Ag, Marinomonas was the primary bacterial group involved in early stage biofilm formation, whereas Anaerospora was primarily involved in late-stage biofilm formation. These results indicate that silver nanoparticles will be unrelated to the bacterial composition of biofilms on the surface of silane-based coatings, while they control biofilm formation there.

ACS Style

Akiko Ogawa; Keito Takakura; Katsuhiko Sano; Hideyuki Kanematsu; Takehiko Yamano; Toshikazu Saishin; Satoshi Terada. Microbiome Analysis of Biofilms of Silver Nanoparticle-Dispersed Silane-Based Coated Carbon Steel Using a Next-Generation Sequencing Technique. Antibiotics 2018, 7, 91 .

AMA Style

Akiko Ogawa, Keito Takakura, Katsuhiko Sano, Hideyuki Kanematsu, Takehiko Yamano, Toshikazu Saishin, Satoshi Terada. Microbiome Analysis of Biofilms of Silver Nanoparticle-Dispersed Silane-Based Coated Carbon Steel Using a Next-Generation Sequencing Technique. Antibiotics. 2018; 7 (4):91.

Chicago/Turabian Style

Akiko Ogawa; Keito Takakura; Katsuhiko Sano; Hideyuki Kanematsu; Takehiko Yamano; Toshikazu Saishin; Satoshi Terada. 2018. "Microbiome Analysis of Biofilms of Silver Nanoparticle-Dispersed Silane-Based Coated Carbon Steel Using a Next-Generation Sequencing Technique." Antibiotics 7, no. 4: 91.

Book chapter
Published: 01 October 2018 in Processing of High Temperature Superconductors
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This chapter focuses on E‐coli and examines the biofilm formation behavior on surfaces of titanium alloys using the own unique laboratory biofilm reactors. The evaluation of biofilms was carried out by the combination of optical microscope, Raman spectroscopy, and staining by crystal violet in an experiment. Specimens were placed on the observation stage and photos were taken at many different places around the focal point. Then all of the photos were integrated into a 2D map. Typical surface profiles show bumpy surfaces in micrometer orders or more. Concave‐convex profiles of biofilms are shown in terms of color differences. The red color sections correspond to convex portions and blue colors to concave ones for the apparatus used in the experiment. The surface organic matters, corresponding to the organic constituents (EPS), were analyzed and representative peaks for polysaccharides, proteins, lipids, nucleic acids were obtained.

ACS Style

Hideyuki Kanematsu; Shun Kanesaki; Hikonaru Kudara; Dana M. Barry; Akiko Ogawa; Takeshi Kougo; Daisuke Kuroda; Nobumitsu Hirai; Hajime Ikegai; Yoshimitsu Mizunoe. Biofilm Formation on Titanium Alloy Surfaces in a Laboratory Biofilm Reactor. Processing of High Temperature Superconductors 2018, 219 -228.

AMA Style

Hideyuki Kanematsu, Shun Kanesaki, Hikonaru Kudara, Dana M. Barry, Akiko Ogawa, Takeshi Kougo, Daisuke Kuroda, Nobumitsu Hirai, Hajime Ikegai, Yoshimitsu Mizunoe. Biofilm Formation on Titanium Alloy Surfaces in a Laboratory Biofilm Reactor. Processing of High Temperature Superconductors. 2018; ():219-228.

Chicago/Turabian Style

Hideyuki Kanematsu; Shun Kanesaki; Hikonaru Kudara; Dana M. Barry; Akiko Ogawa; Takeshi Kougo; Daisuke Kuroda; Nobumitsu Hirai; Hajime Ikegai; Yoshimitsu Mizunoe. 2018. "Biofilm Formation on Titanium Alloy Surfaces in a Laboratory Biofilm Reactor." Processing of High Temperature Superconductors , no. : 219-228.

Book chapter
Published: 01 October 2018 in Processing of High Temperature Superconductors
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This chapter focuses on the correlation between applied elastic waves and biofilm formation in a circulating type of laboratory biofilm reactor (LBR). Several metallic specimens were inserted into the LBR and elastic waves were applied to them for a certain period of time in an experiment. The other method of measurement was the staining test using crystal violet agents. This chemical can stain bacteria themselves and also EPS. Specimens were immersed in 0.1% crystal violet solutions for 30 minutes. The intermediate colors refer to heights between the highest and lowest and they change according to the height. The slight sea‐island patterns were observed when the ultrasonic waves were applied. Since the optical microscopic observation was a local measurement, the results suggest that biofilm still formed at some places on the aluminum surface. Chromium plated specimens were not affected by sonic waves. The extent of violet colors did not change in the case with and without sonic waves. The same tendency was also observed for the glass specimens.

ACS Style

Hideyuki Kanematsu; Shogo Maeda; Dana M. Barry; Senshin Umeki; Kazuyuki Tohji; Nobumitsu Hirai; Akiko Ogawa; Takeshi Kogo; Hajime Ikegai; Yoshimitsu Mizunoe. Effects of Elastic Waves at Several Frequencies on Biofilm Formation in Circulating Types of Laboratory Biofilm Reactors. Processing of High Temperature Superconductors 2018, 43 -51.

AMA Style

Hideyuki Kanematsu, Shogo Maeda, Dana M. Barry, Senshin Umeki, Kazuyuki Tohji, Nobumitsu Hirai, Akiko Ogawa, Takeshi Kogo, Hajime Ikegai, Yoshimitsu Mizunoe. Effects of Elastic Waves at Several Frequencies on Biofilm Formation in Circulating Types of Laboratory Biofilm Reactors. Processing of High Temperature Superconductors. 2018; ():43-51.

Chicago/Turabian Style

Hideyuki Kanematsu; Shogo Maeda; Dana M. Barry; Senshin Umeki; Kazuyuki Tohji; Nobumitsu Hirai; Akiko Ogawa; Takeshi Kogo; Hajime Ikegai; Yoshimitsu Mizunoe. 2018. "Effects of Elastic Waves at Several Frequencies on Biofilm Formation in Circulating Types of Laboratory Biofilm Reactors." Processing of High Temperature Superconductors , no. : 43-51.

Journal article
Published: 28 August 2018 in Procedia Computer Science
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Students’ psychological responses were evaluated by a commercial electrooculography (EOG) sensor. As psychological responses, we picked up two factors – concentration power and the impression about the simplicity for problem solving. A glass-type EOG sensor detected the electrooculogram and calculated the blink rate (focus), the blink strength (calmness) and the head posture (posture), constituting the final parameter called concentration power. In this study, we investigated the correlation between the two psychological responses and EOG parameters. We could confirm the qualitative correlation between the EOG parameter corresponding to concentration power and the psychological response. At this point, we presume that it could be applied to e-learning in virtual classrooms soon. As for the impression about the simplicity to solve problems, we could not found the reasonable correlation. However, the detailed analyses for all of the parameters and their time-dependent behaviors might make it clearer and also other relations with more psychological response.

ACS Style

Hideyuki Kanematsu; Dana M. Barry; Nobuyuki Ogawa; Katsuko T. Nakahira; Michiko Yoshitake; Tatsuya Shirai; Masashi Kawaguchi; Toshiro Kobayashi; Kuniaki Yajima. Some Psychological Responses Measured by a Commercial Electrooculography Sensor and Its Applicability. Procedia Computer Science 2018, 126, 1014 -1022.

AMA Style

Hideyuki Kanematsu, Dana M. Barry, Nobuyuki Ogawa, Katsuko T. Nakahira, Michiko Yoshitake, Tatsuya Shirai, Masashi Kawaguchi, Toshiro Kobayashi, Kuniaki Yajima. Some Psychological Responses Measured by a Commercial Electrooculography Sensor and Its Applicability. Procedia Computer Science. 2018; 126 ():1014-1022.

Chicago/Turabian Style

Hideyuki Kanematsu; Dana M. Barry; Nobuyuki Ogawa; Katsuko T. Nakahira; Michiko Yoshitake; Tatsuya Shirai; Masashi Kawaguchi; Toshiro Kobayashi; Kuniaki Yajima. 2018. "Some Psychological Responses Measured by a Commercial Electrooculography Sensor and Its Applicability." Procedia Computer Science 126, no. : 1014-1022.