The timely knowledge of forage quality of grasslands is vital for matching the demands in animal feeding. Remote sensing (RS) is a promising tool for estimating field-scale forage quality compared with traditional methods, which usually do not provide equally detailed information. However, the applicability of RS prediction models depends on the variability of the underlying calibration data, which can be brought about by the inclusion of a multitude of grassland types and management practices in the model development. Major aims of this study were (i) to build forage quality estimation models for multiple grassland types based on an unmanned aerial vehicle (UAV)-borne imaging spectroscopy and (ii) to generate forage quality distribution maps using the best models obtained. The study examined data from eight grasslands in northern Hesse, Germany, which largely differed in terms of vegetation type and cutting regime. The UAV with a hyperspectral camera on board was utilised to acquire spectral images from the grasslands, and crude protein (CP) and acid detergent fibre (ADF) concentration of the forage was assessed at each cut. Five predictive modelling regression algorithms were applied to develop quality estimation models. Further, grassland forage quality distribution maps were created using the best models developed. The normalised spectral reflectance data showed the strongest relationship with both CP and ADF concentration. From all predictive algorithms, support vector regression provided the highest precision and accuracy for CP estimation (median normalised root mean square error prediction (nRMSE_p) = 10.6%), while cubist regression model proved best for ADF estimation (median nRMSE_p = 13.4%). The maps generated for both CP and ADF showed a distinct spatial variation in forage quality values for the different grasslands and cutting regimes. Overall, the results disclose that UAV-borne imaging spectroscopy, in combination with predictive modelling, provides a promising tool for accurate forage quality estimation of multiple grasslands.

Non‐forest ecosystems, dominated by shrubs, grasses and herbaceous plants, provide ecosystem services including carbon sequestration and forage for grazing, and are highly sensitive to climatic changes. Yet these ecosystems are poorly represented in remotely sensed biomass products and are undersampled by in situ monitoring. Current global change threats emphasize the need for new tools to capture biomass change in non‐forest ecosystems at appropriate scales. Here we developed and deployed a new protocol for photogrammetric height using unoccupied aerial vehicle (UAV) images to test its capability for delivering standardized measurements of biomass across a globally distributed field experiment. We assessed whether canopy height inferred from UAV photogrammetry allows the prediction of aboveground biomass (AGB) across low‐stature plant species by conducting 38 photogrammetric surveys over 741 harvested plots to sample 50 species. We found mean canopy height was strongly predictive of AGB across species, with a median adjusted R² of 0.87 (ranging from 0.46 to 0.99) and median prediction error from leave‐one‐out cross‐validation of 3.9%. Biomass per‐unit‐of‐height was similar within but different among, plant functional types. We found that photogrammetric reconstructions of canopy height were sensitive to wind speed but not sun elevation during surveys. We demonstrated that our photogrammetric approach produced generalizable measurements across growth forms and environmental settings and yielded accuracies as good as those obtained from in situ approaches. We demonstrate that using a standardized approach for UAV photogrammetry can deliver accurate AGB estimates across a wide range of dynamic and heterogeneous ecosystems. Many academic and land management institutions have the technical capacity to deploy these approaches over extents of 1–10 ha⁻¹. Photogrammetric approaches could provide much‐needed information required to calibrate and validate the vegetation models and satellite‐derived biomass products that are essential to understand vulnerable and understudied non‐forested ecosystems around the globe.

Knowledge on the spatio-temporal distribution of invasive plant species is vital to maintain biodiversity in grasslands which are threatened by the invasion of such plants and to evaluate the effect of control activities conducted. Manual digitising of aerial images with field verification is the standard method to create maps of the invasiveLupinus polyphyllusLindl. (Lupine) in semi-natural grasslands of the UNESCO biosphere reserve “Rhön”. As the standard method is labour-intensive, a workflow was developed to map lupine coverage using an unmanned aerial vehicle (UAV)-borne remote sensing (RS) along with object-based image analysis (OBIA). UAV-borne red, green, blue and thermal imaging, as well as photogrammetric canopy height modelling (CHM) were applied. Images were segmented by unsupervised parameter optimisation into image objects representing lupine plants and grass vegetation. Image objects obtained were classified using random forest classification modelling based on objects’ attributes. The classification model was employed to create lupine distribution maps of test areas, and predicted data were compared with manually digitised lupine coverage maps. The classification models yielded a mean prediction accuracy of 89%. The maximum difference in lupine area between classified and digitised lupine maps was 5%. Moreover, the pixel-wise map comparison showed that 88% of all pixels matched between classified and digitised maps. Our results indicated that lupine coverage mapping using UAV-borne RS data and OBIA provides similar results as the standard manual digitising method and, thus, offers a valuable tool to map invasive lupine on grasslands.

Remote sensing data fusion is a powerful tool to gain information of quantitative and qualitative vegetation properties on field level. The aim of this study was to develop prediction models from sensor data fusion for fresh and dry matter yield (FMY/DMY) in extensively managed grasslands with variable degree of invasion by Lupinus polyphyllus. Therefore, a terrestrial 3d laser scanner (TLS) and a drone‐based hyperspectral camera was used to collect high resolution 3d point clouds and hyperspectral aerial orthomosaics of four extremely heterogenous grasslands. From 3d point clouds multiple features (vegetation height, sum of voxel, point density and surface structure) were extracted and combined with hyperspectral data to develop an optimized biomass model from random forest regression algorithm to predict FMY and DMY (n_train = 130, n_test = 33). Models from hyperspectral data solitarily had the lowest prediction performance (FMY: R² = 0.61, nRMSE_r = 17.14; DMY: R² = 0.59, nRMSE_r = 19.37). Higher performance was gained by models derived from 3d laser data (FMY: R² = 0. 76, nRMSE_r = 13.3; DMY: R² = 0. 74, nRMSE_r = 15.1). A fusion of both sensor systems increased the FMY prediction performance up to R² = 0.8; nRMSE_r = 12.02 and the DMY prediction performance to R² = 0.81 and nRMSE_r = 12.06. The fusion of complementary sensor systems can increase the power to predict biomass yields of heterogenous and extensively managed grasslands. It is a novel alternative to labour‐intensive, traditional biomass prediction methods and to remote sensing methods using only single sensor data.

Grassland ecosystems can be hotspots of biodiversity and act as carbon sinks while at the same time providing the basis of forage production for ruminants in dairy and meat production. Annual grassland dry matter yield (DMY) is one of the most important agronomic parameters reflecting differences in usage intensity such as number of harvests and fertilization. Current methods for grassland DMY estimation are labor-intensive and prone to error due to small sample size. With the advent of unmanned aerial vehicles (UAVs) and miniaturized hyperspectral sensors, a novel tool for remote sensing of grassland with high spatial, temporal and radiometric resolution and coverage is available. The present study aimed at developing a robust model capable of estimating grassland biomass across a gradient of usage intensity throughout one growing season. Therefore, UAV-borne hyperspectral data from eight grassland sites in North Hesse, Germany, originating from different harvests, were utilized for the modeling of fresh matter yield (FMY) and DMY. Four machine learning (ML) algorithms were compared for their modeling performance. Among them, the rule-based ML method Cubist regression (CBR) performed best, delivering high prediction accuracies for both FMY (nRMSE_p 7.6%, $R_p^2$ 0.87) and DMY (nRMSE_p 12.9%, $R_p^2$ 0.75). The model showed a high robustness across sites and harvest dates. The best models were employed to produce maps for FMY and DMY, enabling the detailed analysis of spatial patterns. Although the complexity of the approach still restricts its practical application in agricultural management, the current study proved that biomass of grassland sites being subject to different management intensities can be modeled from UAV-borne hyperspectral data at high spatial resolution with high prediction accuracies.