The timely knowledge of forage quality of grasslands is vital for matching the demands in animal feeding. Remote sensing (RS) is a promising tool for estimating field-scale forage quality compared with traditional methods, which usually do not provide equally detailed information. However, the applicability of RS prediction models depends on the variability of the underlying calibration data, which can be brought about by the inclusion of a multitude of grassland types and management practices in the model development. Major aims of this study were (i) to build forage quality estimation models for multiple grassland types based on an unmanned aerial vehicle (UAV)-borne imaging spectroscopy and (ii) to generate forage quality distribution maps using the best models obtained. The study examined data from eight grasslands in northern Hesse, Germany, which largely differed in terms of vegetation type and cutting regime. The UAV with a hyperspectral camera on board was utilised to acquire spectral images from the grasslands, and crude protein (CP) and acid detergent fibre (ADF) concentration of the forage was assessed at each cut. Five predictive modelling regression algorithms were applied to develop quality estimation models. Further, grassland forage quality distribution maps were created using the best models developed. The normalised spectral reflectance data showed the strongest relationship with both CP and ADF concentration. From all predictive algorithms, support vector regression provided the highest precision and accuracy for CP estimation (median normalised root mean square error prediction (nRMSE_p) = 10.6%), while cubist regression model proved best for ADF estimation (median nRMSE_p = 13.4%). The maps generated for both CP and ADF showed a distinct spatial variation in forage quality values for the different grasslands and cutting regimes. Overall, the results disclose that UAV-borne imaging spectroscopy, in combination with predictive modelling, provides a promising tool for accurate forage quality estimation of multiple grasslands.

Agroforestry systems (AFS) can provide positive ecosystem services while at the same time stabilizing yields under increasingly common drought conditions. The effect of distance to trees in alley cropping AFS on yield-related crop parameters has predominantly been studied using point data from transects. Unmanned aerial vehicles (UAVs) offer a novel possibility to map plant traits with high spatial resolution and coverage. In the present study, UAV-borne red, green, blue (RGB) and multispectral imagery was utilized for the prediction of whole crop dry biomass yield (DM) and leaf area index (LAI) of barley at three different conventionally managed silvoarable alley cropping agroforestry sites located in Germany. DM and LAI were modelled using random forest regression models with good accuracies (DM: R² 0.62, nRMSE_p 14.9%, LAI: R² 0.92, nRMSE_p 7.1%). Important variables for prediction included normalized reflectance, vegetation indices, texture and plant height. Maps were produced from model predictions for spatial analysis, showing significant effects of distance to trees on DM and LAI. Spatial patterns differed greatly between the sampled sites and suggested management and soil effects overriding tree effects across large portions of 96 m wide crop alleys, thus questioning alleged impacts of AFS tree rows on yield distribution in intensively managed barley populations. Models based on UAV-borne imagery proved to be a valuable novel tool for prediction of DM and LAI at high accuracies, revealing spatial variability in AFS with high spatial resolution and coverage.

Grassland ecosystems can be hotspots of biodiversity and act as carbon sinks while at the same time providing the basis of forage production for ruminants in dairy and meat production. Annual grassland dry matter yield (DMY) is one of the most important agronomic parameters reflecting differences in usage intensity such as number of harvests and fertilization. Current methods for grassland DMY estimation are labor-intensive and prone to error due to small sample size. With the advent of unmanned aerial vehicles (UAVs) and miniaturized hyperspectral sensors, a novel tool for remote sensing of grassland with high spatial, temporal and radiometric resolution and coverage is available. The present study aimed at developing a robust model capable of estimating grassland biomass across a gradient of usage intensity throughout one growing season. Therefore, UAV-borne hyperspectral data from eight grassland sites in North Hesse, Germany, originating from different harvests, were utilized for the modeling of fresh matter yield (FMY) and DMY. Four machine learning (ML) algorithms were compared for their modeling performance. Among them, the rule-based ML method Cubist regression (CBR) performed best, delivering high prediction accuracies for both FMY (nRMSE_p 7.6%, $R_p^2$ 0.87) and DMY (nRMSE_p 12.9%, $R_p^2$ 0.75). The model showed a high robustness across sites and harvest dates. The best models were employed to produce maps for FMY and DMY, enabling the detailed analysis of spatial patterns. Although the complexity of the approach still restricts its practical application in agricultural management, the current study proved that biomass of grassland sites being subject to different management intensities can be modeled from UAV-borne hyperspectral data at high spatial resolution with high prediction accuracies.

Accurate and timely forage yield prediction in alfalfa-grass mixtures (AGM) is essential for supporting precision agriculture management decisions. This study aimed to develop and evaluate UAV-borne remote sensing models to predict total dry matter yield (DMY) and legume dry matter yield (LY) across multiple harvests and field sites. UAV-borne high-resolution true-colour images were used to derive canopy height models via structure-from-motion. At the same time, multispectral imagery enabled the calculation of reflectance-based vegetation indices. Biomass was destructively sampled, and DMY and LY were determined through drying and botanical fractioning. A total of 276 biomass samples were collected over four harvests, including samples from three AGM fields. To predict DMY and LY, two machine learning regression models (random forest and extreme gradient boosting) were trained and validated using leave-spatial-temporal-group-out cross-validation to ensure robustness across locations and time. Random forest models using fused spectral and height data achieved the best performance, with median prediction errors of 0.51 t ha⁻¹ for DMY (median R² = 0.49) and 0.40 t ha⁻¹ for LY (median R² = 0.65), demonstrating good generalizability under varying agronomic conditions. The study highlights the potential of combining UAV-borne height and spectral data for high-resolution yield mapping in complex forage systems. Predictive maps of DMY and LY provide spatial insights that can inform management and support sustainable nitrogen cycling in crop rotations.