In order to provide a theoretical basis for the thermal design of the neutron production target, flow and heat transfer characteristics are studied by using numerical simulations and experiments. A rectangular mini-channel experimental model consistent with the geometric shape of the heat dissipation structure of neutron production target was established, in which the aspect ratio and gap thickness of the test channel were 53.8:1 and 1.3 mm, respectively. The experimental results indicate that the critical Re of the mini-channel is between 3500 and 4000, and when Re reaches 21,000, Nu can reach 160. The simulation results are in good agreement with the experimental data, and the numerical simulation method can be used for the variable structure optimization design of the target in the later stage. The relationship between the flow pressure drop of the target mini-channel and the aspect ratio and Re is obtained by numerical simulation. The maximum deviation between the correlation and the experimental value is 6%.