Moringa oleifera is nowadays raising as the most preferred medicinal plant, as every part of the moringa plant has potential bioactive compounds which can be used as herbal medicines. Some bioactive compounds of M. oleifera possess potential anti-cancer properties which interact with the apoptosis protein p53 in cancer cell lines of oral squamous cell carcinoma. This research work focuses on the interaction among the selected bioactive compounds derived from M. oleifera with targeted apoptosis protein p53 from the apoptosis pathway to check whether the bioactive compound will induce apoptosis after the mutation in p53. To check the toxicity and drug-likeness of the selected bioactive compound derived from M. oleifera based on Lipinski’s Rule of Five. Detailed analysis of the 3D structure of apoptosis protein p53. To analyze protein’s active site by CASTp 3.0 server. Molecular docking and binding affinity were analyzed between protein p53 with selected bioactive compounds in order to find the most potential inhibitor against the target. This study shows the docking between the potential bioactive compounds with targeted apoptosis protein p53. Quercetin was the most potential bioactive compound whereas kaempferol shows poor affinity towards the targeted p53 protein in the apoptosis pathway. Thus, the objective of this research can provide an insight prediction towards M. oleifera derived bioactive compounds and target apoptosis protein p53 in the structural analysis for compound isolation and in-vivo experiments on the cancer cell line.

Marine algae have gained substantial attention from various scientific and commercial fields. The reason behind its importance is due to the rich source of bioactive compounds like proteins, carbohydrates, lipids, and other pigments. Marine algae-derived carrageenans polysaccharides are used in food, medicine, cosmetic items, gelling, emulsifying, and stabilizing agents. The carrageenan has also photoprotective activity as it protects against the UVB-induced apoptosis in HaCaT cells and inhibits skin aging and cancers. Marine algae-derived macromolecules including polysaccharides and proteins have anticoagulant and photoprotective activities. The marine algae used in health sector is not limited to a food supplement only, rather, the derivatives from it are increasingly used in the biomedical application too. Different biological activities of marine algae as immunomodulatory, anti-tumor, anti-viral, anti-cancer, anti-aging, anti-oxidant, and anti-diabetic are discussed in this review. This mini-review is comprehensively based on the bioactivities materials from brown, red, green, and blue-green algae used in various biomedical fields.

Background: Despite recent advances in the diagnosis and treatment of oral cancer, relative survival rates have not changed significantly. Moringa oleifera (M. oleifera) Lam. is one such plant with its anticancer properties being proved in its leaves, stem, flowers but no studies are yet reported proving the anticancer property of its seed oil on oral cancer. This study aimed to evaluate the anti-proliferative and cytotoxic effect of M. oleifera seed oil against two Oral squamous cell carcinoma cell lines CAL27 and SCC15 using MTT assay. Results: 2D GC-TOF Mass spectrometry revealed a total of 199 compounds, among which the majority were alkanes (68.2016%) and fatty acid esters (11.1399%). The MTT assay report showed good dose-dependent activity. A significant reduction in cell viability within 24 h with IC50 value of 17.78 µg/mL and 24.28 µg/mL for all treatment groups was observed for both the cell lines CAL27 and SCC15. Conclusion: MTT assay showed a significant decrease in cell viability with an increase in the oil dose, thereby revealing the cytotoxic and anti-proliferative activity of M. oleifera seed oil on oral cancer cell lines namely CAL27and SCC15. The results of this study indicate that M. oleifera seed oil can be used as a potent anti-cancer agent in the treatment of Oral cancer. Graphical

Moringa oleifera is a considerable ethnomedical herb with various bioactive compounds. This study aimed to analyze the efficacy of M. oleifera in the prevention and management of various oral conditions. A thorough search was conducted on the Web of Science, Scopus, PubMed, and PubMed Central databases. After screening the data on the basis of inclusion and exclusion criteria, 9 studies were considered for further meta-analysis. The analysis was performed on R programming software (version R-4.0.2) and the results were represented by a forest plot. The estimate obtained via common and random effects model for in vitro studies was statistically insignificant (I2 test P > 0.05) with risk ratios of 8.25 (95% CI: 3.76-18.08) and 7.98 (95% CI: 3.64-17.50) and for in vivo studies were statistically significant (I2 test P )0.05 < with risk ratios of 1.12 (95% CI: 0.90-1.40) and 0.97 (95% CI: 0.71-1.32), indicating the efficacy of M. oleifera in oral diseases on animal and clinical trials, whereas it failed to report the efficacy on in vitro level. Future research has to be done to come up with new and more phytoactive compounds from all parts of the plant with proper extraction procedures. The effectiveness of the compounds has to be validated first on in vitro scale followed by clinical trials so that M. oleifera can be used as therapy in preventing and managing oral ailments.