Background: Probiotics have been used in livestock production for many years, but information on their benefits during the early life of calves is inconsistent. This study aimed to assess the effects of probiotics on the performance of pre-weaning dairy calves and identify the factors influencing their effect sizes. Results: Forty-nine studies were selected for meta-analysis based on the inclusion and exclusion criteria. The study qualities were evaluated using a predefined risk assessment tool following GRADE guidelines. Meta-analysis results showed that probiotics increased the growth performance (body weight by 1.988 kg and average daily gain by 40.689 g/d), decreased digestibility and feed efficiency (feed conversion rate by 0.073), altered rumen parameter (decreased acetate by 2.815 mmol/L and increased butyrate by 0.788 mmol/L), altered blood parameter (decreased AST by 4.188 U/L, increased BHBA by 0.029 mmol/L and IgG by 0.698 g/L), increased faecal parameter (faecal bacteria counts by 0.680 log₁₀ CFU/g), based on the strict criteria (P_SMD < 0.05, I² < 50%). Additionally, probiotics increased digestibility and feed efficiency (starter dry matter intake by 0.034 kg/d and total dry matter intake by 0.020 kg/d), altered blood parameter (increased IgA by 0.313 g/L, IgM by 0.262 g/L, and total antioxidant capacity by 0.441 U/mL, decreased MDA by 0.404 nmol/mL), decreased faecal parameter (faecal score by 0.052), based on the loose criteria (P_SMD < 0.05, I² > 50%). Regression and sub-group analyses showed that probiotic strains, supplementation dosage, and methods significantly affected the performance of calves. The probiotics supplied with more than 9.5 log₁₀ CFU/d significantly increased IgA and IgM contents (P_SMD < 0.05). Additionally, the compound probiotics significantly increased TDMI, IgA, and IgM (P_SMD ≤ 0.001). Furthermore, probiotics supplemented in liquid (whole milk or milk replacer) significantly increased TDMI and decreased faecal score (P_SMD < 0.05), while in whole milk, they significantly increased body weight, IgA, and IgM (P_SMD < 0.001). Conclusions: Probiotics could improve the growth performance, feed intake and efficiency, rumen fermentation, immune and antioxidant capacity, and health of pre-weaning calves. However, the effect sizes were related to the dosage, composition, and supplementation methods of probiotics.

Perilipin 1 (PLIN1) is a protein encoded by the PLIN1 gene in eukaryotes. PLIN1 is a member of the PAT protein family, a family of proteins related to lipid droplet (LD) surface proteins. PLIN1 phosphorylation plays a vital role during fat metabolism of adipose tissue lipolysis and fat storage in adipocytes. However, to further explore the regulation of the PLIN1 gene on the proliferation, differentiation and lipid metabolism of bovine adipocytes. In this study, the mRNA expression of PLIN1, at day six, was the highest during bovine adipocyte differentiation. Moreover, PLIN1 can promote the proliferation and differentiation of preadipocytes in cattle. On the sixth day, after transfection with, and overexpression of, the PLIN1 gene in bovine preadipocytes via adenovirus, cell samples were collected, and transcriptome sequencing was performed. A total of 1923 differentially expressed genes were detected. Through GO and KEGG pathway analysis, the differentially expressed genes were established to be mainly enriched in the AMPK, Wnt, and PPAR signaling pathways related to fat proliferation and differentiation. In conclusion, at the transcriptional level, PLIN1 plays an important role in regulating fat proliferation and metabolism. Additionally, the sequencing results screened new differentially expressed genes related to fat metabolism, providing theoretical support for molecular breeding of Qinchuan beef cattle.

Although numerous studies investigated the effect of UASMS2 polymorphism in leptin gene on cattle production, a consensus has not yet been reached. Therefore, we reviewed and meta-analyzed the effects of UASMS2 on cattle. We searched potentially relevant studies from seven databases (to December 25, 2019). Standard mean difference along with 95% confidence intervals was calculated to assess the strength of association through the random-effects model. Six published articles containing 1378 cattle samples were included in our meta-analysis. We found UASMS2 was not related to carcass weight, dressing percentage and loin muscle area in the recessive genetic model, but there was a significant association between UASMS2 and average daily weight gain, dry matter intake, body weight, marbling score, and backfat thickness. This meta-analysis indicated that UASMS2 was associated with growth and meat quality traits of cattle, implying that this SNP can be used reliably in beef cattle breeding. This study may provide valuable information on improving beef yield and quality in cattle production.

FATP1 plays an important role in the regulation of fatty acid metabolism and lipid accumulation. In this study, we investigated the patterns of FATP1 expression in various tissues obtained from calf and adult Qinchuan cattle, and in differentiating adipocytes. Next, we investigated the effect of FATP1 expression on preadipocyte differentiation in Qinchuan cattle using overexpression and interference assays. We also identified the differentially expressed genes (DEGs) and pathways associated with FATP1 overexpression/interference. Our results reveal that FATP1 was broadly expressed in heart, kidney, muscle, small intestine, large intestine, and perirenal fat tissues. While FATP1 overexpression promoted preadipocyte differentiation, fat deposition, and the expression of several genes involved in fat metabolism, FATP1 interference had the opposite effects on adipocyte differentiation. Following FATP1 overexpression and FATP1 interference in adipocytes, RNA-seq analysis was performed to identify DEGs related to fat metabolism. The DEGs identified include SLPI, STC1, SEMA6A, TNFRSF19, SLN, PTGS2, ADCYP1, FADS2, and SCD. Pathway analysis revealed that the DEGs were enriched in the PPAR signaling pathway, AMPK signal pathway, and Insulin signaling pathway. Our results provide an in-depth understanding of the function and regulation mechanism of FAPT1 in fat metabolism.

Background: Probiotics have been used in livestock production for many years, but information on their benefits during the early life of calves is inconsistent. This study aimed to assess the effects of probiotics on the performance of pre-weaning dairy calves and identify the factors influencing their effect size. Results: Forty-nine studies were selected for meta-analysis based on the inclusion and exclusion criteria. The study qualities were evaluated using a predefined risk assessment tool following GRADE guidelines. Meta-analysis results showed that probiotics increased the body weight by 1.988 kg, average daily gain by 40.689 g/d, butyrate by 0.788 mmol/L, BHBA by 0.029 mmol/L, and IgG by 0.698 g/L, based on the strict criteria (P_SMD < 0.05, I² < 50%). In contrast, probiotics decreased feed conversion rate by 0.073, acetate by 2.815 mmol/L, AST by 4.188 U/L (P_SMD < 0.05, I² < 50%). Additionally, probiotics increased starter dry matter intake by 0.034 kg/d, total dry matter intake (TDMI) by 0.020 kg/d, IgA by 0.313 g/L, IgM by 0.262 g/L, total antioxidant capacity by 0.441 U/mL, and faecal bacteria counts by 0.680 log₁₀ CFU/g, based on the loose criteria (P_SMD < 0.05, I² > 50%). However, probiotics decreased LDH by 78.796 U/L, MDA by 0.404 nmol/mL, and faecal score by 0.052 (P_SMD < 0.05, I² > 50%). Regression and sub-group analyses showed that probiotic strains, supplementary dosage, and method significantly affected the performance of calves. The probiotics supplied with more than 9.5 log₁₀ CFU/d significantly increased IgA and IgG contents (P_SMD < 0.05). Additionally, the compound probiotics significantly increased TDMI, IgA, and IgM (P_SMD < 0.001). Furthermore, probiotics supplemented in liquid (whole milk or milk replacer) significantly increased TDMI and decreased faecal score (P_SMD < 0.05), while in whole milk, they significantly increased body weight, IgA, and IgM (P_SMD < 0.001). Conclusions: Probiotics improve the growth performance, feed intake and efficiency, rumen fermentation, immune and antioxidant capacity, and health of pre-weaning calves. However, the effect sizes were related to the dosage, composition, and supplementary method of probiotics.