Reverse osmosis (RO) desalination is considered a viable alternative to reduce water scarcity; however, its energy consumption is high. Photovoltaic (PV) energy in desalination processes has gained popularity in recent years. The temperature is identified as a variable that directly affects the behavior of different parameters of the RO process and energy production in PV panels. The objective of this study was to evaluate the effect of temperature on energy consumption and polarization factor in desalination processes at 20, 23, 26 and 30 °C. Tests were conducted on a RO desalination plant driven by a fixed 24-module PV system that received spray cooling in the winter, spring and summer seasons. The specific energy consumption was lower with increasing process feed temperature, being 4.4, 4.3, 3.9 and 3.5 kWh m⁻³ for temperatures of 20, 23, 26 and 30 °C, respectively. The water temperature affected the polarization factor, being lower as the temperature increased. The values obtained were within the limits established as optimal to prevent the formation of scaling on the membrane surface. The spray cooling system was able to decrease the temperature of the solar cells by about 6.2, 13.3 and 11.5 °C for the winter, spring and summer seasons, respectively. The increase in energy production efficiency was 7.96–14.25%, demonstrating that solar cell temperature control is a viable alternative to improve power generation in solar panel systems.