Phomopsis juglandina (Sacc.) Höhn., which is the conidial state of Diaporthe juglandina (Fuckel) Nitschke, and the main pathogen causing the dieback of branches and twigs of walnut was recently detected in many orchards from Romania. The symptomatological, morphological, ultrastructural, and cultural characteristics, as well as the pathogenicity of an isolate of this lignicolous fungus, were described and illustrated. The optimum periods for infection, under the conditions prevailing in Southern Romania, mainly occur in the spring (April) and autumn months (late September-beginning of October). Strong inverse correlations (p < 0.001) were found between potential evapotranspiration and lesion lengths on walnut branches in 2019. The pathogen forms two types of phialospores: alpha and beta; the role of beta phialospores is not well known in pathogenesis. In Vitro, the optimal growth temperature of mycelial hyphae was in the range of 22–26 °C, and the optimal pH is 4.4–7. This pathogen should be monitored continuously due to its potential for damaging infestations of intensive plantations.
Wet deposition is influencing air quality because air pollutants are washed away from the surrounding air. Consequently, particulate matter and associated compounds are transported in the rainwater and enter into soil, surface waters, and groundwater. Nonpoint sources of heavy metals from stormwater runoff have increased in urban areas due to industrialization and the increasing impervious surfaces. In this work, we present an assessment of the rainwater composition regarding the nutrients and other physicochemical characteristics measured in three locations selected in Targoviste city, Romania, a city that had a specialized steel factory and important metallurgical facilities. The rainwater was collected using three PALMEX rain samplers and then was transferred to high-density polyethylene bottles and analyzed using ICP-MS. PM2.5 concentrations were also monitored continuously using optical monitors calibrated using a gravimetric sampler. A detailed analysis of the heavy metals content in rainwater and PM was presented for the pollution episodes occurring in October and November 2019. Backward trajectories were computed using the HYSPLIT model for these periods. The results showed that the PM2.5 ranged from 11.1 to 24.1 μg/m3 in 2019, while the heavy metals in collected rainwater were (µg L−1): 0.25 (Cd) − CV = 26.5%, 0.10 (Co) − CV = 58.1%, 1.77 (Cr) − CV = 24.3%, 377.37 (Ni) − CV = 27.9%, 0.67 (Pb) − CV = 74.3%, and 846.5 (Zn) − CV = 20.6%. Overall, Ni, Pb, Cr, and V had significant correlations between the concentrations from rainwater and PM. Negative associations were found between precipitation events and heavy metals both from rainwater and PM, but only a few showed statistical significance. However, this could explain the “washing” effect of the rain on the heavy metals from PM2.5. The potential sources of nitrogen in the rainwater collected in Targoviste could be from burning fossil fuels and the soils, including both biological processes and fertilization resulting from the intensive agriculture in the piedmont plain in which the city is located. Based on the results, rainwater monitoring can constitute a reliable method for air quality characterization. Additional research is required to better understand seasonality and sources of heterogeneity regarding the associations between PM and rainwater composition.
In recent years, the accelerated development of the remote sensing domain and the improvement of the resolution and frequency of satellite images allowed the increase in the accuracy of the evaluation of morphometric characteristics and the spatiotemporal distribution of pit lakes, including the small ones. Our study quantitatively analyzes small-scale pit lakes in the piedmont and subsidence plains from contact with the Getic and Curvature Subcarpathians from Romania using the normalized difference water index (NDWI) and data series, with different resolutions, from Landsat 8, Google Earth, and Sentinel 2A. The problems encountered in extracting the contours of the gravel pit lakes were determined by the different resolution of the images, the uneven quality of the images exported from Google Earth, and an additional challenge was given by the diversity of the analyzed land surfaces, the land use, and the optical properties of the lakes. A comparison of the obtained NDWI values using data series from Sentinel 2A and Landsat 8 highlighted the importance of resolution and also showed a larger spectral difference between the identified water bodies and the surrounding land in favor of Sentinel 2A. Regarding the vegetation-derived indices, superior leaf area index (1.8–3) was recorded in low-lying plains and mixed areas (tall shrubs, wetlands, etc.) because the river banks have increased moisture that supports taller species with denser foliage and the sparsely vegetated areas are located in agricultural crops and in/near villages. Changes in vegetation richness and abundance can be spatiotemporally monitored using indices derived from the spectral bands of satellite imagery.