Within the framework of energy biomass production, Arundo donax L. is very promising for its capability to grow on marginal lands with high yields. This potential can be realized in unused polluted areas where the energy production can be coupled with phytoremediation, and harvested biomass represents a resource and a means to remove contaminants from the soil. Two main processes are considered to evaluate A. donax L. biomass as an energy crop, determined by the timing of harvest: anaerobic digestion with fresh biomass before winter and combustion (e.g., pyrolysis and gasification) of dry canes in late winter. The aim of this work was to evaluate the use of A. donax L. in an area polluted by heavy metals for phytoextraction and energy production at two different harvest times (October and February). For that purpose, we established in polluted area in northern Italy (Caffaro area, Brescia) an experimental field of A. donax, and included switchgrass (Panicum virgatum L.) and mixed meadow species as controls. The results obtained by ICP-MS analysis performed on harvested biomasses highlighted a differential uptake of heavy metals depending on harvest time. In particular, considering the yield in the third year, A. donax was able to remove from the soil 3.87 kg ha⁻¹ of Zn, 2.09 kg ha⁻¹ of Cu and 0.007 kg ha⁻¹ of Cd when harvested in October. Production of A. donax L. for anaerobic digestion or combustion in polluted areas represents a potential solution for both energy production and phytoextraction of heavy metals, in particular Cu, Zn and Cd.

In recent years, the interest in increasingly sustainable agriculture has also turned attention towards new cover crops suitable for use in marginal areas that could enter the food chain as new protein and oil sources or for biodiesel production. In this scenario, Camelina sativa is a perfect crop to study. Camelina is an annual herbaceous plant belonging to the Brassicaceae which is interesting in terms of its oil content, since the seeds contain about 40% oils, with a high level of polyunsaturated fatty acids (30–40% alpha linolenic acid, 15–25% linoleic acid, 15% oleic acid and about 15% eicosenoic acid). It is a hexaploid species (2n = 40, genome size ~782 Mb) characterized by rapid growth, a short life cycle (85–100 days for spring varieties, 190–210 for autumn varieties) and low input cultivation needs. However, its use in feed and food is limited by the presence of glucosinolates (GLS). GLS are sulfur molecules involved in plant defense. In recent years, they have been studied not only as antinutritionals but also for their anti-carcinogenic effects against chronic inflammatory and heart diseases and for their use as natural pesticides. Given the recent interest in camelina and its highly nutritious oil, eight pure lines and a synthetic population were compared in two different growing periods, spring and winter. In this work, the genetic materials were characterized for different phenotypic traits, yields and yield components, and bromatological and glucosinolate content. The results confirmed that in North Italy, camelina has higher yields if cultivated in the autumn–winter period (about 2 t/ha vs. 0.6 t/ha); furthermore, a negative correlation was found between spring and winter yields, indicating that varieties that produce more in winter cultivation produce less in spring cultivation. Moreover, to our knowledge, it is the first work in which a synthetic population of Camelina sativa has been tested and proved to be a valid solution for use in various environments both for its adaptability and for the low content of glucosinolates (about 17 mmol/kg).

The introduction of mechanized agricultural practices after the Second World War and the use of productive hybrids led to a gradual disappearance of local maize varieties. However, 13 landraces are still cultivated in North-Western Italy, in the Lombardy region; those that are cultivated in mountainous areas (roughly up to 1200 m in altitude) are often characterized by the pointed shape of their seeds (i.e., “Nero Spinoso”, “Rostrato Rosso di Rovetta”, “Spinato di Gandino” and “Scagliolo di Carenno”) and the presence of pigments (i.e., “Nero Spinoso”, “Rostrato Rosso di Rovetta”). The pointed shape of the seeds is an ancient characteristic of maize-ancestors, which negatively affects the yield by not allowing optimal “filling” of the ear. This study reports work on four different Italian varieties of pointed maize in order to assess the genetic bases of the “pointed character” and to try to explain the reasons for this adaptation to the mountain environment. The data obtained by genetic analysis, seed air-drying modeling and thermographic camera observations demonstrated that the “pointed trait” is controlled by the same genes across the different varieties studied and suggested that this peculiar shape has been selected in mountainous areas because it promotes faster drying of the seed, with the presence of pigments implementing this effect.

Phytic acid (PA) is a strong anti-nutritional factor with a key antioxidant role in countering reactive oxygen species. Despite the potential benefits of low phytic acid (lpa) mutants, the reduction of PA causes pleiotropic effects, e.g., reduced seed germination and viability loss related to seed ageing. The current study evaluated a historical series of naturally aged seeds and showed that lpa1-1 seeds aged faster as compared to wildtype. To mimic natural ageing, the present study set up accelerated ageing treatments at different temperatures. It was found that incubating the seeds at 57 °C for 24 h, the wildtype germinated at 82.4% and lpa1-1 at 40%. The current study also hypothesized two possible solutions to overcome these problems: (1) Classical breeding was used to constitute synthetic populations carrying the lpa1-1 mutation, with genes pushing anthocyanin accumulation in the embryo (R-navajo allele). The outcome showed that the presence of R-navajo in the lpa1-1 genotype was not able to improve the germinability (−20%), but this approach could be useful to improve the germinability in non-mutant genotypes (+17%). (2) In addition, hydropriming was tested on lpa1-1 and wildtype seeds, and germination was improved by 20% in lpa1-1, suggesting a positive role of seed priming in restoring germination. Moreover, the data highlighted metabolic differences in the metabolome before and after hydropriming treatment, suggesting that the differences in germination could also be mediated by differences in the metabolic composition induced by the mutation.

The optimization of artificial pollination is crucial in breeding programs for allogamous plants. In maize, achieving a balance between the labor-intensive nature of controlled pollinations and the need for large-scale production of hybrid seeds, along with considerations of germinability and pollen production, determines the success of genetic improvement programs. Breeding programs in maize have resulted in a reduction in the number of tassel branches to increase light interception and plant density in production fields. However, despite this genetic improvement, the decreased pollen production per plant has raised critical concerns regarding pollination and subsequent ear filling, especially under adverse environmental conditions. The aim of this work was the analysis of factors that can contribute to increasing the efficiency of controlled pollination in maize. The data obtained showed that pollen diameter, flavonoid, and phenolic acid content do not influence the percentage of germination and therefore the efficiency of pollination. The quantity of pollen is a central factor in ensuring the efficiency of controlled pollinations, and the data obtained by comparing traditional varieties with modern hybrids of maize showed that an increase in pollen production is determined by the increase in branching of the male inflorescence. Furthermore, we propose the use of a “smart” pollination method to make this step in maize breeding programs easier and faster.

The cuticle is the plant’s outermost layer that covers the surfaces of aerial parts. This structure is composed of a variety of aliphatic molecules and is well-known for its protective role against biotic and abiotic stresses in plants. Mutants with a permeable cuticle show developmental defects such as organ fusions and altered seed germination and viability. In this study, we identified a novel maize mutant, stocky1, with unique features: lethal at the seedling stage, and showing a severely dwarfed phenotype, due to a defective cuticle. For the first time, the mutant was tentatively mapped to chromosome 5, bin 5.04. The mutant phenotype investigated in this work has the potential to contribute to the elucidation of the role of the cuticle during plant development. The possibility of controlling this trait is of relevance in the context of climate change, as it may contribute to tolerance to abiotic stresses.